P32854 (PEP12_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Syntaxin PEP12

Alternative name(s):
Carboxypeptidase Y-deficient protein 12
Vacuolar protein sorting-associated protein 6
Vacuolar protein-targeting protein 13

Gene names

Name:	PEP12
Synonyms:	VPS6, VPT13
Ordered Locus Names:	YOR036W
ORF Names:	OR26.29

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in the sorting and targeting of vacuolar proteases.
Subcellular location	Membrane; Single-pass type IV membrane protein Potential.
Post-translational modification	Ubiquitinated. Ref.4
Sequence similarities	Belongs to the syntaxin family. Contains 1 t-SNARE coiled-coil homology domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Coiled coil Transmembrane Transmembrane helix
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Golgi to vacuole transport Inferred from mutant phenotype Ref.1. Source: SGD intracellular protein transport Inferred from electronic annotation. Source: InterPro vacuole inheritance Inferred from mutant phenotype. Source: SGD
Cellular component	Golgi apparatus Inferred from mutant phenotype Ref.1. Source: SGD endosome Inferred from mutant phenotype Ref.1. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	SNAP receptor activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
YKT6	P36015	2	EBI-13098,EBI-26982

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	Syntaxin PEP12		PRO_0000210271
Regions
	Topological domain	1 – 268	268	Cytoplasmic Potential
	Transmembrane	269 – 288	20	Helical; Anchor for type IV membrane protein; Potential
	Domain	195 – 257	63	t-SNARE coiled-coil homology
Amino acid modifications
	Modified residue	2	1	Phosphoserine Ref.5 Ref.6
	Modified residue	23	1	Phosphoserine Ref.6
Experimental info
	Mutagenesis	271	1	L → D: Increases ubiquitination by TUL1. Ref.4
	Sequence conflict	13	1	A → G in AAB38370. Ref.1
	Sequence conflict	89	1	V → I in AAB38370. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P32854 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: D9F5711339DA1CDA	FASTA	288	33,006
	10 20 30 40 50 60 MSEDEFFGGD NEAVWNGSRF SDSPEFQTLK EEVAAELFEI NGQISTLQQF TATLKSFIDR 70 80 90 100 110 120 GDVSAKVVER INKRSVAKIE EIGGLIKKVN TSVKKMDAIE EASLDKTQII AREKLVRDVS 130 140 150 160 170 180 YSFQEFQGIQ RQFTQVMKQV NERAKESLEA SEMANDAALL DEEQRQNSSK STRIPGSQIV 190 200 210 220 230 240 IERDPINNEE FAYQQNLIEQ RDQEISNIER GITELNEVFK DLGSVVQQQG VLVDNIEANI 250 260 270 280 YTTSDNTQLA SDELRKAMRY QKRTSRWRVY LLIVLLVMLL FIFLIMKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Novel syntaxin homologue, Pep12p, required for the sorting of lumenal hydrolases to the lysosome-like vacuole in yeast." Becherer K.A., Rieder S.E., Emr S.D., Jones E.W. Mol. Biol. Cell 7:579-594(1996) [PubMed: 8730101] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"A transmembrane ubiquitin ligase required to sort membrane proteins into multivesicular bodies." Reggiori F., Pelham H.R.B. Nat. Cell Biol. 4:117-123(2002) [PubMed: 11788821] [Abstract] Cited for: UBIQUITINATION BY TUL1, MUTAGENESIS OF LEU-271.
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2, MASS SPECTROMETRY. Strain: ADR376.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2 AND SER-23, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M90395 Genomic DNA. Translation: AAB38370.1. X87331 Genomic DNA. Translation: CAA60755.1. Z74944 Genomic DNA. Translation: CAA99226.1. BK006948 Genomic DNA. Translation: DAA10819.1.
PIR	S62175.
RefSeq	NP_014679.1. NM_001183455.1.
3D structure databases
ProteinModelPortal	P32854.
SMR	P32854. Positions 200-265.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2021N.
IntAct	P32854. 13 interactions.
MINT	MINT-389382.
STRING	P32854.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR036W; YOR036W; YOR036W.
GeneID	854201.
KEGG	sce:YOR036W.
NMPDR	fig|4932.3.peg.5779.
Organism-specific databases
CYGD	YOR036w.
SGD	S000005562. PEP12.
Phylogenomic databases
eggNOG	fuNOG12527.
GeneTree	EFGT00050000002424.
HOGENOM	HBG202813.
OMA	ASEMAND.
OrthoDB	EOG4ZKNWR.
Gene expression databases
ArrayExpress	P32854.
Genevestigator	P32854.
GermOnline	YOR036W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006012. Syntaxin/epimorphin_CS. IPR006011. Syntaxin_N. IPR010989. t-SNARE. IPR000727. T_SNARE_dom. [Graphical view]
KO	K08488.
Pfam	PF05739. SNARE. 1 hit. [Graphical view]
SMART	SM00503. SynN. 1 hit. SM00397. t_SNARE. 1 hit. [Graphical view]
SUPFAM	SSF47661. t-snare. 1 hit.
PROSITE	PS00914. SYNTAXIN. 1 hit. PS50192. T_SNARE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	976038.

Entry information

Entry name

PEP12_YEAST

Accession

Primary (citable) accession number: P32854
Secondary accession number(s): D6W2A3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program