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P32851

- STX1A_RAT

UniProt

P32851 - STX1A_RAT

Protein

Syntaxin-1A

Gene

Stx1a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 146 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca2+-regulation of exocytosis acrosomal reaction in sperm.

    GO - Molecular functioni

    1. ATP-dependent protein binding Source: RGD
    2. calcium channel inhibitor activity Source: Ensembl
    3. calcium-dependent protein binding Source: RGD
    4. glycoprotein binding Source: MGI
    5. myosin binding Source: RGD
    6. myosin head/neck binding Source: RGD
    7. protein binding Source: UniProtKB
    8. protein binding, bridging Source: RGD
    9. protein domain specific binding Source: RGD
    10. protein heterodimerization activity Source: RGD
    11. protein N-terminus binding Source: RGD
    12. SNARE binding Source: MGI

    GO - Biological processi

    1. calcium ion-dependent exocytosis Source: Ensembl
    2. intracellular protein transport Source: InterPro
    3. neurotransmitter transport Source: RGD
    4. positive regulation of exocytosis Source: RGD
    5. positive regulation of neurotransmitter secretion Source: RGD
    6. regulation of exocytosis Source: RGD
    7. response to gravity Source: RGD
    8. synaptic vesicle docking involved in exocytosis Source: UniProtKB

    Keywords - Biological processi

    Exocytosis, Neurotransmitter transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_227883. Glutamate Neurotransmitter Release Cycle.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Syntaxin-1A
    Alternative name(s):
    Neuron-specific antigen HPC-1
    Synaptotagmin-associated 35 kDa protein
    Short name:
    P35A
    Gene namesi
    Name:Stx1a
    Synonyms:Sap
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 12

    Organism-specific databases

    RGDi69430. Stx1a.

    Subcellular locationi

    GO - Cellular componenti

    1. actomyosin Source: RGD
    2. cell junction Source: UniProtKB-KW
    3. integral component of membrane Source: UniProtKB
    4. intracellular organelle Source: RGD
    5. neuron projection Source: UniProtKB-SubCell
    6. plasma membrane Source: RGD
    7. protein complex Source: RGD
    8. secretory granule Source: UniProtKB
    9. SNARE complex Source: UniProtKB
    10. synaptic vesicle membrane Source: UniProtKB-SubCell
    11. synaptobrevin 2-SNAP-25-syntaxin-1a complex Source: MGI
    12. synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex Source: MGI
    13. synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex Source: RGD

    Keywords - Cellular componenti

    Cell junction, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 288288Syntaxin-1APRO_0000210189Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei14 – 141PhosphoserineBy similarity
    Modified residuei188 – 1881Phosphoserine; by DAPK11 Publication

    Post-translational modificationi

    Phosphorylated by CK2. Phosphorylation at Ser-188 by DAPK1 significantly decreases its interaction with STXBP1.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP32851.
    PRIDEiP32851.

    PTM databases

    PhosphoSiteiP32851.

    Miscellaneous databases

    PMAP-CutDBP32851.

    Expressioni

    Tissue specificityi

    Expressed predominantly in cerebral cortex, hippocampus, cerebellum, adrenal medulla and retina with weak expression detected in non-neuronal tissues.

    Gene expression databases

    GenevestigatoriP32851.

    Interactioni

    Subunit structurei

    Interacts with OTOF, SYTL4, VAPA, SYBU and LGI3. Found in a complex with VAMP8 and SNAP23. Interacts with SYT6 and SYT8; the interaction is Ca2+-dependent By similarity. Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds to CPLX1. Binds STXBP6. Found in a ternary complex with STX1A and SNAP25. Interacts with SLC6A4. Interacts with STXBP1 and DAPK1.By similarity10 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Apba1O354303EBI-539720,EBI-704760
    Snap25P6088110EBI-539720,EBI-1027214
    Stxbp1P6176515EBI-539720,EBI-1029097
    Vamp2P630455EBI-539720,EBI-520880

    Protein-protein interaction databases

    BioGridi250448. 11 interactions.
    DIPiDIP-108N.
    IntActiP32851. 13 interactions.
    MINTiMINT-96097.
    STRINGi10116.ENSRNOP00000040699.

    Structurei

    Secondary structure

    1
    288
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni6 – 83
    Helixi28 – 6336
    Beta strandi64 – 663
    Helixi69 – 10436
    Turni105 – 1073
    Helixi111 – 14636
    Helixi162 – 1709
    Helixi176 – 1805
    Beta strandi183 – 1853
    Helixi192 – 25463
    Helixi261 – 28424

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1BR0NMR-A27-144[»]
    1EZ3X-ray1.90A/B/C24-150[»]
    1HVVX-ray2.40A/B/C/D190-264[»]
    1JTHX-ray2.00B/D191-267[»]
    1KILX-ray2.30B191-250[»]
    1N7SX-ray1.45B191-256[»]
    1SFCX-ray2.40B/F/J180-262[»]
    1URQX-ray2.00B188-259[»]
    2BU0model-B191-258[»]
    2M8RNMR-A183-288[»]
    3C98X-ray2.60B1-267[»]
    3HD7X-ray3.40B/F183-288[»]
    3IPDX-ray4.80B/F183-288[»]
    3RK2X-ray2.20B/F191-253[»]
    3RK3X-ray3.50B191-253[»]
    3RL0X-ray3.80B/F/J/N/R/V/Z/d191-253[»]
    4JEHX-ray2.50B25-266[»]
    4JEUX-ray3.20B2-243[»]
    DisProtiDP00155.
    ProteinModelPortaliP32851.
    SMRiP32851. Positions 2-259.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP32851.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 265265CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei266 – 28823Helical; Anchor for type IV membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini192 – 25463t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili68 – 10942Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi13 – 197Asp-rich (acidic)

    Sequence similaritiesi

    Belongs to the syntaxin family.Curated
    Contains 1 t-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5074.
    GeneTreeiENSGT00730000110552.
    HOGENOMiHOG000286023.
    HOVERGENiHBG000497.
    InParanoidiP32851.
    KOiK04560.
    OMAiHEETKAV.
    OrthoDBiEOG7X9G7R.
    PhylomeDBiP32851.
    TreeFamiTF313763.

    Family and domain databases

    InterProiIPR028669. STX1A/1B.
    IPR006012. Syntaxin/epimorphin_CS.
    IPR006011. Syntaxin_N.
    IPR010989. t-SNARE.
    IPR000727. T_SNARE_dom.
    [Graphical view]
    PANTHERiPTHR19957:SF84. PTHR19957:SF84. 1 hit.
    PfamiPF05739. SNARE. 1 hit.
    PF00804. Syntaxin. 1 hit.
    [Graphical view]
    SMARTiSM00503. SynN. 1 hit.
    SM00397. t_SNARE. 1 hit.
    [Graphical view]
    SUPFAMiSSF47661. SSF47661. 1 hit.
    PROSITEiPS00914. SYNTAXIN. 1 hit.
    PS50192. T_SNARE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32851-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKDRTQELRT AKDSDDDDDV TVTVDRDRFM DEFFEQVEEI RGFIDKIAEN    50
    VEEVKRKHSA ILASPNPDEK TKEELEELMS DIKKTANKVR SKLKSIEQSI 100
    EQEEGLNRSS ADLRIRKTQH STLSRKFVEV MSEYNATQSD YRERCKGRIQ 150
    RQLEITGRTT TSEELEDMLE SGNPAIFASG IIMDSSISKQ ALSEIETRHS 200
    EIIKLENSIR ELHDMFMDMA MLVESQGEMI DRIEYNVEHA VDYVERAVSD 250
    TKKAVKYQSK ARRKKIMIII CCVILGIIIA STIGGIFG 288
    Length:288
    Mass (Da):33,067
    Last modified:October 1, 1993 - v1
    Checksum:i8F929F489E323CAA
    GO

    Sequence cautioni

    The sequence BAA01231.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10392 mRNA. Translation: BAA01231.1. Different initiation.
    D12519 mRNA. Translation: BAA02089.1.
    M95734 mRNA. Translation: AAA42195.1.
    PIRiA38141.
    RefSeqiNP_446240.2. NM_053788.2.
    UniGeneiRn.9943.

    Genome annotation databases

    EnsembliENSRNOT00000049601; ENSRNOP00000040699; ENSRNOG00000029165.
    GeneIDi116470.
    KEGGirno:116470.
    UCSCiRGD:69430. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10392 mRNA. Translation: BAA01231.1 . Different initiation.
    D12519 mRNA. Translation: BAA02089.1 .
    M95734 mRNA. Translation: AAA42195.1 .
    PIRi A38141.
    RefSeqi NP_446240.2. NM_053788.2.
    UniGenei Rn.9943.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1BR0 NMR - A 27-144 [» ]
    1EZ3 X-ray 1.90 A/B/C 24-150 [» ]
    1HVV X-ray 2.40 A/B/C/D 190-264 [» ]
    1JTH X-ray 2.00 B/D 191-267 [» ]
    1KIL X-ray 2.30 B 191-250 [» ]
    1N7S X-ray 1.45 B 191-256 [» ]
    1SFC X-ray 2.40 B/F/J 180-262 [» ]
    1URQ X-ray 2.00 B 188-259 [» ]
    2BU0 model - B 191-258 [» ]
    2M8R NMR - A 183-288 [» ]
    3C98 X-ray 2.60 B 1-267 [» ]
    3HD7 X-ray 3.40 B/F 183-288 [» ]
    3IPD X-ray 4.80 B/F 183-288 [» ]
    3RK2 X-ray 2.20 B/F 191-253 [» ]
    3RK3 X-ray 3.50 B 191-253 [» ]
    3RL0 X-ray 3.80 B/F/J/N/R/V/Z/d 191-253 [» ]
    4JEH X-ray 2.50 B 25-266 [» ]
    4JEU X-ray 3.20 B 2-243 [» ]
    DisProti DP00155.
    ProteinModelPortali P32851.
    SMRi P32851. Positions 2-259.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 250448. 11 interactions.
    DIPi DIP-108N.
    IntActi P32851. 13 interactions.
    MINTi MINT-96097.
    STRINGi 10116.ENSRNOP00000040699.

    PTM databases

    PhosphoSitei P32851.

    Proteomic databases

    PaxDbi P32851.
    PRIDEi P32851.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000049601 ; ENSRNOP00000040699 ; ENSRNOG00000029165 .
    GeneIDi 116470.
    KEGGi rno:116470.
    UCSCi RGD:69430. rat.

    Organism-specific databases

    CTDi 6804.
    RGDi 69430. Stx1a.

    Phylogenomic databases

    eggNOGi COG5074.
    GeneTreei ENSGT00730000110552.
    HOGENOMi HOG000286023.
    HOVERGENi HBG000497.
    InParanoidi P32851.
    KOi K04560.
    OMAi HEETKAV.
    OrthoDBi EOG7X9G7R.
    PhylomeDBi P32851.
    TreeFami TF313763.

    Enzyme and pathway databases

    Reactomei REACT_227883. Glutamate Neurotransmitter Release Cycle.

    Miscellaneous databases

    EvolutionaryTracei P32851.
    NextBioi 619018.
    PMAP-CutDB P32851.
    PROi P32851.

    Gene expression databases

    Genevestigatori P32851.

    Family and domain databases

    InterProi IPR028669. STX1A/1B.
    IPR006012. Syntaxin/epimorphin_CS.
    IPR006011. Syntaxin_N.
    IPR010989. t-SNARE.
    IPR000727. T_SNARE_dom.
    [Graphical view ]
    PANTHERi PTHR19957:SF84. PTHR19957:SF84. 1 hit.
    Pfami PF05739. SNARE. 1 hit.
    PF00804. Syntaxin. 1 hit.
    [Graphical view ]
    SMARTi SM00503. SynN. 1 hit.
    SM00397. t_SNARE. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47661. SSF47661. 1 hit.
    PROSITEi PS00914. SYNTAXIN. 1 hit.
    PS50192. T_SNARE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequence analysis of cDNA for a neuronal cell membrane antigen, HPC-1."
      Inoue A., Obata K., Akagawa K.
      J. Biol. Chem. 267:10613-10619(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 8-37 AND 44-102.
      Tissue: Brain.
    2. "HPC-1 is associated with synaptotagmin and omega-conotoxin receptor."
      Yoshida A., Oho C., Omori A., Kuwahara R., Ito T., Takahashi M.
      J. Biol. Chem. 267:24925-24928(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-288, PROTEIN SEQUENCE OF 1-15 AND 95-113.
      Tissue: Brain.
    3. "Syntaxin: a synaptic protein implicated in docking of synaptic vesicles at presynaptic active zones."
      Bennett M.K., Calakos N., Scheller R.H.
      Science 257:255-259(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-288, PROTEIN SEQUENCE OF 4-16 AND 31-56.
      Tissue: Brain.
    4. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    5. "Syntaxin 12, a member of the syntaxin family localized to the endosome."
      Tang B.L., Tan A.E., Lim L.K., Lee S.S., Low D.Y., Hong W.
      J. Biol. Chem. 273:6944-6950(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SNAP23.
    6. "Mixed and non-cognate SNARE complexes. Characterization of assembly and biophysical properties."
      Fasshauer D., Antonin W., Margittai M., Pabst S., Jahn R.
      J. Biol. Chem. 274:15440-15446(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A TERNARY COMPLEX WITH VAMP8 AND SNAP25.
    7. "Regulation of the serotonin transporter by interacting proteins."
      Haase J., Killian A.M., Magnani F., Williams C.
      Biochem. Soc. Trans. 29:722-728(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SLC6A4.
    8. "Amisyn, a novel syntaxin-binding protein that may regulate SNARE complex assembly."
      Scales S.J., Hesser B.A., Masuda E.S., Scheller R.H.
      J. Biol. Chem. 277:28271-28279(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH STXBP6.
    9. "Ca2+-dependent phosphorylation of syntaxin-1A by the death-associated protein (DAP) kinase regulates its interaction with Munc18."
      Tian J.H., Das S., Sheng Z.H.
      J. Biol. Chem. 278:26265-26274(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-188, INTERACTION WITH DAPK1 AND STKB1.
    10. "Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 A resolution."
      Sutton R.B., Fasshauer D., Jahn R., Brunger A.T.
      Nature 395:347-353(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 180-262 IN COMPLEX WITH SNAP25 AND VAMP2.
    11. "Structural analysis of the neuronal SNARE protein syntaxin-1A."
      Lerman J.C., Robblee J., Fairman R., Hughson F.M.
      Biochemistry 39:8470-8479(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 24-150.
    12. "Three-dimensional structure of the neuronal-Sec1-syntaxin 1a complex."
      Misura K.M.S., Scheller R.H., Weis W.I.
      Nature 404:355-362(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 27-248 IN COMPLEX WITH STXBP1.
    13. "Self-association of the H3 region of syntaxin 1A. Implications for intermediates in SNARE complex assembly."
      Misura K.M.S., Scheller R.H., Weis W.I.
      J. Biol. Chem. 276:13273-13282(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 191-267.
    14. "Crystal structure and biophysical properties of a complex between the N-terminal SNARE region of SNAP25 and syntaxin 1a."
      Misura K.M.S., Gonzalez L.C. Jr., May A.P., Scheller R.H., Weis W.I.
      J. Biol. Chem. 276:41301-41309(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 191-267 IN COMPLEX WITH SNAP25.
    15. "Three-dimensional structure of the complexin/SNARE complex."
      Chen X., Tomchick D.R., Kovrigin E., Arac D., Machius M., Suedhof T.C., Rizo J.
      Neuron 33:397-409(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 191-253 IN COMPLEX WITH CPLX1; SNAP25 AND VAMP2, STRUCTURE BY NMR.
    16. "High resolution structure, stability, and synaptotagmin binding of a truncated neuronal SNARE complex."
      Ernst J.A., Brunger A.T.
      J. Biol. Chem. 278:8630-8636(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS) OF 191-256 IN COMPLEX WITH SNAP25 AND VAMP2.
    17. "Three-dimensional structure of an evolutionarily conserved N-terminal domain of syntaxin 1A."
      Fernandez I., Ubach J., Dulubova I., Zhang X., Suedhof T.C., Rizo J.
      Cell 94:841-849(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 27-146.

    Entry informationi

    Entry nameiSTX1A_RAT
    AccessioniPrimary (citable) accession number: P32851
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 146 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3