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P32851

- STX1A_RAT

UniProt

P32851 - STX1A_RAT

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Protein

Syntaxin-1A

Gene

Stx1a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca2+-regulation of exocytosis acrosomal reaction in sperm.

GO - Molecular functioni

  1. ATP-dependent protein binding Source: RGD
  2. calcium channel inhibitor activity Source: Ensembl
  3. calcium-dependent protein binding Source: RGD
  4. glycoprotein binding Source: MGI
  5. myosin binding Source: RGD
  6. myosin head/neck binding Source: RGD
  7. protein binding, bridging Source: RGD
  8. protein domain specific binding Source: RGD
  9. protein heterodimerization activity Source: RGD
  10. protein N-terminus binding Source: RGD
  11. SNARE binding Source: MGI

GO - Biological processi

  1. calcium ion-dependent exocytosis Source: Ensembl
  2. intracellular protein transport Source: InterPro
  3. neurotransmitter transport Source: RGD
  4. positive regulation of exocytosis Source: RGD
  5. positive regulation of neurotransmitter secretion Source: RGD
  6. regulation of exocytosis Source: RGD
  7. response to gravity Source: RGD
  8. synaptic vesicle docking involved in exocytosis Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Exocytosis, Neurotransmitter transport, Transport

Enzyme and pathway databases

ReactomeiREACT_227883. Glutamate Neurotransmitter Release Cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Syntaxin-1A
Alternative name(s):
Neuron-specific antigen HPC-1
Synaptotagmin-associated 35 kDa protein
Short name:
P35A
Gene namesi
Name:Stx1a
Synonyms:Sap
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 12

Organism-specific databases

RGDi69430. Stx1a.

Subcellular locationi

GO - Cellular componenti

  1. actomyosin Source: RGD
  2. cell junction Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB
  4. intracellular organelle Source: RGD
  5. neuron projection Source: UniProtKB-KW
  6. plasma membrane Source: RGD
  7. protein complex Source: RGD
  8. secretory granule Source: UniProtKB
  9. SNARE complex Source: UniProtKB
  10. synaptic vesicle membrane Source: Ensembl
  11. synaptobrevin 2-SNAP-25-syntaxin-1a complex Source: MGI
  12. synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex Source: MGI
  13. synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 288288Syntaxin-1APRO_0000210189Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei14 – 141PhosphoserineBy similarity
Modified residuei188 – 1881Phosphoserine; by DAPK11 Publication

Post-translational modificationi

Phosphorylated by CK2. Phosphorylation at Ser-188 by DAPK1 significantly decreases its interaction with STXBP1.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP32851.
PRIDEiP32851.

PTM databases

PhosphoSiteiP32851.

Miscellaneous databases

PMAP-CutDBP32851.

Expressioni

Tissue specificityi

Expressed predominantly in cerebral cortex, hippocampus, cerebellum, adrenal medulla and retina with weak expression detected in non-neuronal tissues.

Gene expression databases

GenevestigatoriP32851.

Interactioni

Subunit structurei

Interacts with OTOF, SYTL4, VAPA, SYBU and LGI3. Found in a complex with VAMP8 and SNAP23. Interacts with SYT6 and SYT8; the interaction is Ca2+-dependent (By similarity). Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds to CPLX1. Binds STXBP6. Found in a ternary complex with STX1A and SNAP25. Interacts with SLC6A4. Interacts with STXBP1 and DAPK1.By similarity10 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Apba1O354303EBI-539720,EBI-704760
Snap25P6088110EBI-539720,EBI-1027214
Stxbp1P6176515EBI-539720,EBI-1029097
Vamp2P630455EBI-539720,EBI-520880

Protein-protein interaction databases

BioGridi250448. 11 interactions.
DIPiDIP-108N.
IntActiP32851. 13 interactions.
MINTiMINT-96097.
STRINGi10116.ENSRNOP00000040699.

Structurei

Secondary structure

1
288
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni6 – 83
Helixi28 – 6336
Beta strandi64 – 663
Helixi69 – 10436
Turni105 – 1073
Helixi111 – 14636
Helixi162 – 1709
Helixi176 – 1805
Beta strandi183 – 1853
Helixi192 – 25463
Helixi261 – 28424

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BR0NMR-A27-144[»]
1EZ3X-ray1.90A/B/C24-150[»]
1HVVX-ray2.40A/B/C/D190-264[»]
1JTHX-ray2.00B/D191-267[»]
1KILX-ray2.30B191-250[»]
1N7SX-ray1.45B191-256[»]
1SFCX-ray2.40B/F/J180-262[»]
1URQX-ray2.00B188-259[»]
2BU0model-B191-258[»]
2M8RNMR-A183-288[»]
3C98X-ray2.60B1-267[»]
3HD7X-ray3.40B/F183-288[»]
3IPDX-ray4.80B/F183-288[»]
3RK2X-ray2.20B/F191-253[»]
3RK3X-ray3.50B191-253[»]
3RL0X-ray3.80B/F/J/N/R/V/Z/d191-253[»]
4JEHX-ray2.50B25-266[»]
4JEUX-ray3.20B2-243[»]
DisProtiDP00155.
ProteinModelPortaliP32851.
SMRiP32851. Positions 2-259.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32851.

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 265265CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei266 – 28823Helical; Anchor for type IV membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini192 – 25463t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili68 – 10942Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi13 – 197Asp-rich (acidic)

Sequence similaritiesi

Belongs to the syntaxin family.Curated
Contains 1 t-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5074.
GeneTreeiENSGT00760000119200.
HOGENOMiHOG000286023.
HOVERGENiHBG000497.
InParanoidiP32851.
KOiK04560.
OMAiHEETKAV.
OrthoDBiEOG7X9G7R.
PhylomeDBiP32851.
TreeFamiTF313763.

Family and domain databases

InterProiIPR028669. STX1A/1B.
IPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PANTHERiPTHR19957:SF84. PTHR19957:SF84. 1 hit.
PfamiPF05739. SNARE. 1 hit.
PF00804. Syntaxin. 1 hit.
[Graphical view]
SMARTiSM00503. SynN. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
PROSITEiPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32851 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKDRTQELRT AKDSDDDDDV TVTVDRDRFM DEFFEQVEEI RGFIDKIAEN
60 70 80 90 100
VEEVKRKHSA ILASPNPDEK TKEELEELMS DIKKTANKVR SKLKSIEQSI
110 120 130 140 150
EQEEGLNRSS ADLRIRKTQH STLSRKFVEV MSEYNATQSD YRERCKGRIQ
160 170 180 190 200
RQLEITGRTT TSEELEDMLE SGNPAIFASG IIMDSSISKQ ALSEIETRHS
210 220 230 240 250
EIIKLENSIR ELHDMFMDMA MLVESQGEMI DRIEYNVEHA VDYVERAVSD
260 270 280
TKKAVKYQSK ARRKKIMIII CCVILGIIIA STIGGIFG
Length:288
Mass (Da):33,067
Last modified:October 1, 1993 - v1
Checksum:i8F929F489E323CAA
GO

Sequence cautioni

The sequence BAA01231.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10392 mRNA. Translation: BAA01231.1. Different initiation.
D12519 mRNA. Translation: BAA02089.1.
M95734 mRNA. Translation: AAA42195.1.
PIRiA38141.
RefSeqiNP_446240.2. NM_053788.2.
UniGeneiRn.9943.

Genome annotation databases

EnsembliENSRNOT00000049601; ENSRNOP00000040699; ENSRNOG00000029165.
GeneIDi116470.
KEGGirno:116470.
UCSCiRGD:69430. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10392 mRNA. Translation: BAA01231.1 . Different initiation.
D12519 mRNA. Translation: BAA02089.1 .
M95734 mRNA. Translation: AAA42195.1 .
PIRi A38141.
RefSeqi NP_446240.2. NM_053788.2.
UniGenei Rn.9943.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1BR0 NMR - A 27-144 [» ]
1EZ3 X-ray 1.90 A/B/C 24-150 [» ]
1HVV X-ray 2.40 A/B/C/D 190-264 [» ]
1JTH X-ray 2.00 B/D 191-267 [» ]
1KIL X-ray 2.30 B 191-250 [» ]
1N7S X-ray 1.45 B 191-256 [» ]
1SFC X-ray 2.40 B/F/J 180-262 [» ]
1URQ X-ray 2.00 B 188-259 [» ]
2BU0 model - B 191-258 [» ]
2M8R NMR - A 183-288 [» ]
3C98 X-ray 2.60 B 1-267 [» ]
3HD7 X-ray 3.40 B/F 183-288 [» ]
3IPD X-ray 4.80 B/F 183-288 [» ]
3RK2 X-ray 2.20 B/F 191-253 [» ]
3RK3 X-ray 3.50 B 191-253 [» ]
3RL0 X-ray 3.80 B/F/J/N/R/V/Z/d 191-253 [» ]
4JEH X-ray 2.50 B 25-266 [» ]
4JEU X-ray 3.20 B 2-243 [» ]
DisProti DP00155.
ProteinModelPortali P32851.
SMRi P32851. Positions 2-259.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 250448. 11 interactions.
DIPi DIP-108N.
IntActi P32851. 13 interactions.
MINTi MINT-96097.
STRINGi 10116.ENSRNOP00000040699.

PTM databases

PhosphoSitei P32851.

Proteomic databases

PaxDbi P32851.
PRIDEi P32851.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000049601 ; ENSRNOP00000040699 ; ENSRNOG00000029165 .
GeneIDi 116470.
KEGGi rno:116470.
UCSCi RGD:69430. rat.

Organism-specific databases

CTDi 6804.
RGDi 69430. Stx1a.

Phylogenomic databases

eggNOGi COG5074.
GeneTreei ENSGT00760000119200.
HOGENOMi HOG000286023.
HOVERGENi HBG000497.
InParanoidi P32851.
KOi K04560.
OMAi HEETKAV.
OrthoDBi EOG7X9G7R.
PhylomeDBi P32851.
TreeFami TF313763.

Enzyme and pathway databases

Reactomei REACT_227883. Glutamate Neurotransmitter Release Cycle.

Miscellaneous databases

EvolutionaryTracei P32851.
NextBioi 619018.
PMAP-CutDB P32851.
PROi P32851.

Gene expression databases

Genevestigatori P32851.

Family and domain databases

InterProi IPR028669. STX1A/1B.
IPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view ]
PANTHERi PTHR19957:SF84. PTHR19957:SF84. 1 hit.
Pfami PF05739. SNARE. 1 hit.
PF00804. Syntaxin. 1 hit.
[Graphical view ]
SMARTi SM00503. SynN. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view ]
SUPFAMi SSF47661. SSF47661. 1 hit.
PROSITEi PS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequence analysis of cDNA for a neuronal cell membrane antigen, HPC-1."
    Inoue A., Obata K., Akagawa K.
    J. Biol. Chem. 267:10613-10619(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 8-37 AND 44-102.
    Tissue: Brain.
  2. "HPC-1 is associated with synaptotagmin and omega-conotoxin receptor."
    Yoshida A., Oho C., Omori A., Kuwahara R., Ito T., Takahashi M.
    J. Biol. Chem. 267:24925-24928(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-288, PROTEIN SEQUENCE OF 1-15 AND 95-113.
    Tissue: Brain.
  3. "Syntaxin: a synaptic protein implicated in docking of synaptic vesicles at presynaptic active zones."
    Bennett M.K., Calakos N., Scheller R.H.
    Science 257:255-259(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-288, PROTEIN SEQUENCE OF 4-16 AND 31-56.
    Tissue: Brain.
  4. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  5. "Syntaxin 12, a member of the syntaxin family localized to the endosome."
    Tang B.L., Tan A.E., Lim L.K., Lee S.S., Low D.Y., Hong W.
    J. Biol. Chem. 273:6944-6950(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SNAP23.
  6. "Mixed and non-cognate SNARE complexes. Characterization of assembly and biophysical properties."
    Fasshauer D., Antonin W., Margittai M., Pabst S., Jahn R.
    J. Biol. Chem. 274:15440-15446(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A TERNARY COMPLEX WITH VAMP8 AND SNAP25.
  7. "Regulation of the serotonin transporter by interacting proteins."
    Haase J., Killian A.M., Magnani F., Williams C.
    Biochem. Soc. Trans. 29:722-728(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SLC6A4.
  8. "Amisyn, a novel syntaxin-binding protein that may regulate SNARE complex assembly."
    Scales S.J., Hesser B.A., Masuda E.S., Scheller R.H.
    J. Biol. Chem. 277:28271-28279(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH STXBP6.
  9. "Ca2+-dependent phosphorylation of syntaxin-1A by the death-associated protein (DAP) kinase regulates its interaction with Munc18."
    Tian J.H., Das S., Sheng Z.H.
    J. Biol. Chem. 278:26265-26274(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-188, INTERACTION WITH DAPK1 AND STKB1.
  10. "Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 A resolution."
    Sutton R.B., Fasshauer D., Jahn R., Brunger A.T.
    Nature 395:347-353(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 180-262 IN COMPLEX WITH SNAP25 AND VAMP2.
  11. "Structural analysis of the neuronal SNARE protein syntaxin-1A."
    Lerman J.C., Robblee J., Fairman R., Hughson F.M.
    Biochemistry 39:8470-8479(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 24-150.
  12. "Three-dimensional structure of the neuronal-Sec1-syntaxin 1a complex."
    Misura K.M.S., Scheller R.H., Weis W.I.
    Nature 404:355-362(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 27-248 IN COMPLEX WITH STXBP1.
  13. "Self-association of the H3 region of syntaxin 1A. Implications for intermediates in SNARE complex assembly."
    Misura K.M.S., Scheller R.H., Weis W.I.
    J. Biol. Chem. 276:13273-13282(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 191-267.
  14. "Crystal structure and biophysical properties of a complex between the N-terminal SNARE region of SNAP25 and syntaxin 1a."
    Misura K.M.S., Gonzalez L.C. Jr., May A.P., Scheller R.H., Weis W.I.
    J. Biol. Chem. 276:41301-41309(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 191-267 IN COMPLEX WITH SNAP25.
  15. "Three-dimensional structure of the complexin/SNARE complex."
    Chen X., Tomchick D.R., Kovrigin E., Arac D., Machius M., Suedhof T.C., Rizo J.
    Neuron 33:397-409(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 191-253 IN COMPLEX WITH CPLX1; SNAP25 AND VAMP2, STRUCTURE BY NMR.
  16. "High resolution structure, stability, and synaptotagmin binding of a truncated neuronal SNARE complex."
    Ernst J.A., Brunger A.T.
    J. Biol. Chem. 278:8630-8636(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS) OF 191-256 IN COMPLEX WITH SNAP25 AND VAMP2.
  17. "Three-dimensional structure of an evolutionarily conserved N-terminal domain of syntaxin 1A."
    Fernandez I., Ubach J., Dulubova I., Zhang X., Suedhof T.C., Rizo J.
    Cell 94:841-849(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 27-146.

Entry informationi

Entry nameiSTX1A_RAT
AccessioniPrimary (citable) accession number: P32851
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: October 29, 2014
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3