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Protein

DNA repair protein RAD5

Gene

RAD5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable helicase, member of the UBC2/RAD6 epistasis group. Functions with the DNA repair protein RAD18 in error-free postreplication DNA repair. Involved in the maintenance of wild-type rates of instability of simple repetitive sequences such as poly(GT) repeats. Seems to be involved in maintaining a balance which acts in favor of error-prone non-homologous joining during DNA double-strand breaks repairs. Recruits the UBC13-MMS2 dimer to chromatin for DNA repair.8 Publications

Cofactori

Kineticsi

  1. KM=525 µM for ATP

    pH dependencei

    Optimum pH is 7.0. for ATPase activity.

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi532 – 539ATPPROSITE-ProRule annotation8
    Zinc fingeri914 – 961RING-typePROSITE-ProRule annotationAdd BLAST48

    GO - Molecular functioni

    • ATP binding Source: UniProtKB-KW
    • DNA-dependent ATPase activity Source: SGD
    • four-way junction DNA binding Source: SGD
    • four-way junction helicase activity Source: SGD
    • Y-form DNA binding Source: SGD
    • zinc ion binding Source: InterPro

    GO - Biological processi

    • double-strand break repair Source: SGD
    • error-prone translesion synthesis Source: SGD
    • free ubiquitin chain polymerization Source: SGD
    • postreplication repair Source: SGD
    • protein polyubiquitination Source: SGD
    Complete GO annotation...

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    ATP-binding, DNA-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32191-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA repair protein RAD5 (EC:3.6.4.-)
    Alternative name(s):
    Radiation sensitivity protein 5
    Revertibility protein 2
    Gene namesi
    Name:RAD5
    Synonyms:REV2, SNM2
    Ordered Locus Names:YLR032W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XII

    Organism-specific databases

    EuPathDBiFungiDB:YLR032W.
    SGDiS000004022. RAD5.

    Subcellular locationi

    GO - Cellular componenti

    • chromosome, telomeric region Source: SGD
    • cytoplasm Source: UniProtKB-SubCell
    • nuclear chromatin Source: SGD
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi538 – 539KT → AA: Increased sensitivity toward ionizing radiation. 1 Publication2
    Mutagenesisi914C → S: Abolishes interaction with UBC13. 2 Publications1
    Mutagenesisi916I → A: Abolishes interaction with UBC13. 1 Publication1
    Mutagenesisi944Y → A: Abolishes interaction with UBC13. 1 Publication1
    Mutagenesisi959N → A: Abolishes interaction with UBC13. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemovedCombined sources
    ChainiPRO_00000561302 – 1169DNA repair protein RAD5Add BLAST1168

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei2N-acetylserineCombined sources1
    Modified residuei20PhosphoserineCombined sources1
    Modified residuei23PhosphoserineCombined sources1
    Modified residuei129PhosphoserineCombined sources1
    Modified residuei130PhosphoserineCombined sources1

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP32849.
    PRIDEiP32849.

    PTM databases

    iPTMnetiP32849.

    Interactioni

    Subunit structurei

    Homodimer. Interacts with POL30, RAD18, UBC9 and UBC13.4 Publications

    Protein-protein interaction databases

    BioGridi31306. 164 interactors.
    DIPiDIP-5830N.
    IntActiP32849. 3 interactors.
    MINTiMINT-2784640.

    Structurei

    3D structure databases

    ProteinModelPortaliP32849.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini519 – 730Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST212
    Domaini995 – 1165Helicase C-terminalPROSITE-ProRule annotationAdd BLAST171

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Motifi681 – 684DEGH box4

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi42 – 60Asp/Glu-rich (acidic)Add BLAST19
    Compositional biasi303 – 315Arg/Lys-rich (basic)Add BLAST13

    Sequence similaritiesi

    Belongs to the SNF2/RAD54 helicase family.Curated
    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
    Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Zinc fingeri914 – 961RING-typePROSITE-ProRule annotationAdd BLAST48

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    GeneTreeiENSGT00860000133848.
    HOGENOMiHOG000040492.
    InParanoidiP32849.
    KOiK15505.
    OMAiMDPWWSP.
    OrthoDBiEOG092C0L0F.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    3.40.50.300. 4 hits.
    InterProiIPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR014905. HIRAN.
    IPR027417. P-loop_NTPase.
    IPR000330. SNF2_N.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR017907. Znf_RING_CS.
    [Graphical view]
    PfamiPF00271. Helicase_C. 1 hit.
    PF08797. HIRAN. 1 hit.
    PF00176. SNF2_N. 1 hit.
    PF13639. zf-RING_2. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    SM00910. HIRAN. 1 hit.
    SM00184. RING. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 4 hits.
    PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS00518. ZF_RING_1. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P32849-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSHIEQEERK RFFNDDLDTS ETSLNFKSEN KESFLFANSH NDDDDDVVVS
    60 70 80 90 100
    VSDTTEGEGD RSIVPVRREI EEEGQNQFIT ELLRIIPEMP KDLVMELNEK
    110 120 130 140 150
    FGSQEEGLSL ALSHYFDHNS GTSISKIPSS PNQLNTLSDT SNSTLSPSSF
    160 170 180 190 200
    HPKRRRIYGF RNQTRLEDKV TWKRFIGALQ VTGMATRPTV RPLKYGSQMK
    210 220 230 240 250
    LKRSSEEISA TKVYDSRGRK KASMASLVRI FDIQYDREIG RVSEDIAQIL
    260 270 280 290 300
    YPLLSSHEIS FEVTLIFCDN KRLSIGDSFI LQLDCFLTSL IFEERNDGES
    310 320 330 340 350
    LMKRRRTEGG NKREKDNGNF GRTLTETDEE LESRSKRLAL LKLFDKLRLK
    360 370 380 390 400
    PILDEQKALE KHKIELNSDP EIIDLDNDEI CSNQVTEVHN NLRDTQHEEE
    410 420 430 440 450
    TMNLNQLKTF YKAAQSSESL KSLPETEPSR DVFKLELRNY QKQGLTWMLR
    460 470 480 490 500
    REQEFAKAAS DGEASETGAN MINPLWKQFK WPNDMSWAAQ NLQQDHVNVE
    510 520 530 540 550
    DGIFFYANLH SGEFSLAKPI LKTMIKGGIL SDEMGLGKTV AAYSLVLSCP
    560 570 580 590 600
    HDSDVVDKKL FDIENTAVSD NLPSTWQDNK KPYASKTTLI VVPMSLLTQW
    610 620 630 640 650
    SNEFTKANNS PDMYHEVYYG GNVSSLKTLL TKTKTPPTVV LTTYGIVQNE
    660 670 680 690 700
    WTKHSKGRMT DEDVNISSGL FSVNFYRIII DEGHNIRNRT TVTSKAVMAL
    710 720 730 740 750
    QGKCKWVLTG TPIINRLDDL YSLVKFLELD PWRQINYWKT FVSTPFESKN
    760 770 780 790 800
    YKQAFDVVNA ILEPVLLRRT KQMKDKDGKP LVELPPKEVV IKRLPFSKSQ
    810 820 830 840 850
    DLLYKFLLDK AEVSVKSGIA RGDLLKKYST ILVHILRLRQ VCCHPGLIGS
    860 870 880 890 900
    QDENDEDLSK NNKLVTEQTV ELDSLMRVVS ERFDNSFSKE ELDAMIQRLK
    910 920 930 940 950
    VKYPDNKSFQ SLECSICTTE PMDLDKALFT ECGHSFCEKC LFEYIEFQNS
    960 970 980 990 1000
    KNLGLKCPNC RNQIDACRLL ALVQTNSNSK NLEFKPYSPA SKSSKITALL
    1010 1020 1030 1040 1050
    KELQLLQDSS AGEQVVIFSQ FSTYLDILEK ELTHTFSKDV AKIYKFDGRL
    1060 1070 1080 1090 1100
    SLKERTSVLA DFAVKDYSRQ KILLLSLKAG GVGLNLTCAS HAYMMDPWWS
    1110 1120 1130 1140 1150
    PSMEDQAIDR LHRIGQTNSV KVMRFIIQDS IEEKMLRIQE KKRTIGEAMD
    1160
    TDEDERRKRR IEEIQMLFE
    Length:1,169
    Mass (Da):134,002
    Last modified:October 1, 1993 - v1
    Checksum:i226B720097433EE2
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti478Q → R in AAB23590 (PubMed:1394508).Curated1
    Sequence conflicti635T → N in AAB23590 (PubMed:1394508).Curated1
    Sequence conflicti846G → S in AAB23590 (PubMed:1394508).Curated1
    Sequence conflicti898R → S in AAB23590 (PubMed:1394508).Curated1
    Sequence conflicti973V → A in AAB23590 (PubMed:1394508).Curated1
    Sequence conflicti1063A → R in AAB23590 (PubMed:1394508).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M96644 Genomic DNA. Translation: AAA34951.1.
    Z73204 Genomic DNA. Translation: CAA97556.1.
    S46103 Genomic DNA. Translation: AAB23590.1.
    BK006945 Genomic DNA. Translation: DAA09350.1.
    PIRiS64859.
    RefSeqiNP_013132.1. NM_001181919.1.

    Genome annotation databases

    EnsemblFungiiYLR032W; YLR032W; YLR032W.
    GeneIDi850719.
    KEGGisce:YLR032W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M96644 Genomic DNA. Translation: AAA34951.1.
    Z73204 Genomic DNA. Translation: CAA97556.1.
    S46103 Genomic DNA. Translation: AAB23590.1.
    BK006945 Genomic DNA. Translation: DAA09350.1.
    PIRiS64859.
    RefSeqiNP_013132.1. NM_001181919.1.

    3D structure databases

    ProteinModelPortaliP32849.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi31306. 164 interactors.
    DIPiDIP-5830N.
    IntActiP32849. 3 interactors.
    MINTiMINT-2784640.

    PTM databases

    iPTMnetiP32849.

    Proteomic databases

    MaxQBiP32849.
    PRIDEiP32849.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYLR032W; YLR032W; YLR032W.
    GeneIDi850719.
    KEGGisce:YLR032W.

    Organism-specific databases

    EuPathDBiFungiDB:YLR032W.
    SGDiS000004022. RAD5.

    Phylogenomic databases

    GeneTreeiENSGT00860000133848.
    HOGENOMiHOG000040492.
    InParanoidiP32849.
    KOiK15505.
    OMAiMDPWWSP.
    OrthoDBiEOG092C0L0F.

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32191-MONOMER.

    Miscellaneous databases

    PROiP32849.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    3.40.50.300. 4 hits.
    InterProiIPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR014905. HIRAN.
    IPR027417. P-loop_NTPase.
    IPR000330. SNF2_N.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR017907. Znf_RING_CS.
    [Graphical view]
    PfamiPF00271. Helicase_C. 1 hit.
    PF08797. HIRAN. 1 hit.
    PF00176. SNF2_N. 1 hit.
    PF13639. zf-RING_2. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    SM00910. HIRAN. 1 hit.
    SM00184. RING. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 4 hits.
    PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS00518. ZF_RING_1. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiRAD5_YEAST
    AccessioniPrimary (citable) accession number: P32849
    Secondary accession number(s): D6VY34
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: November 30, 2016
    This is version 155 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1520 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.