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P32847 (B3AT_ONCMY) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Band 3 anion exchange protein
Alternative name(s):
Anion exchange protein 1
Short name=AE 1
Short name=Anion exchanger 1
Solute carrier family 4 member 1
Gene names
Name:slc4a1
Synonyms:ae1
OrganismOncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Taxonomic identifier8022 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiProtacanthopterygiiSalmoniformesSalmonidaeSalmoninaeOncorhynchus

Protein attributes

Sequence length918 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane protein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the cell membrane. Mediates chloride-bicarbonate exchange across the cell membrane By similarity.

Subunit structure

A dimer in solution, it spans the membrane asymmetrically and appears to be tetrameric By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity. Basolateral cell membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the anion exchanger (TC 2.A.31) family. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 918918Band 3 anion exchange protein
PRO_0000079213

Regions

Topological domain1 – 392392Cytoplasmic Potential
Transmembrane393 – 41321Helical; Potential
Transmembrane426 – 44520Helical; Potential
Transmembrane449 – 46820Helical; Potential
Transmembrane480 – 49920Helical; Potential
Transmembrane512 – 53019Helical; Potential
Topological domain531 – 58151Extracellular Potential
Transmembrane582 – 60120Helical; Potential
Topological domain602 – 61615Cytoplasmic Potential
Transmembrane617 – 63721Helical; Potential
Topological domain638 – 67336Extracellular Potential
Transmembrane674 – 69320Helical; Potential
Transmembrane712 – 73221Helical; Potential
Transmembrane772 – 78918Helical; Potential
Transmembrane794 – 81522Helical; Potential
Transmembrane853 – 87422Helical; Potential
Region393 – 918526Membrane (anion exchange)

Amino acid modifications

Lipidation8521S-palmitoyl cysteine By similarity
Glycosylation5461N-linked (GlcNAc...) Potential
Glycosylation5681N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P32847 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: 37E163141FBDC16A

FASTA918101,894
        10         20         30         40         50         60 
MENDLSFGED VMSYEEESDS AFPSPIRPTP PGHSGNYDLE QSRQEEDSNQ AIQSIVVHTD 

        70         80         90        100        110        120 
PEAYLNLNTN ANTRGDAQAY VELNELMGNS WQETGRWVGY EENFNPGTGK WGPSHVSYLT 

       130        140        150        160        170        180 
FKSLIQLRKI MSTGAIILDL QASSLSAVAE KVVDELRTKG EIRAADRDGL LRALLQRRSQ 

       190        200        210        220        230        240 
SEGAVAQPLG GDIEMQTFSV TKQRDTTDSV EASIVLSGVM DSLEKPAVAF VRLGDSVVIE 

       250        260        270        280        290        300 
GALEAPVPVR FVFVLVGPSQ GGVDYHESGR AMAALMADWV FSLEAYLAPT NKELTNAIAD 

       310        320        330        340        350        360 
FMDCGIVIPP TEIQDEGMLQ PIIDFQKKML KDRLRPSDTR IIFGGGAKAD EADEEPREDP 

       370        380        390        400        410        420 
LARTGIPFGG MIKDMKRRYR HYISDFTDAL DPQVLAAVIF IYFAALSPAI TFGGLLADKT 

       430        440        450        460        470        480 
EHMMGVSELM ISTCVQGIIF AFIAAQPTLV IGFSGPLLVF EEAFFAFCKS QEIEYIVGRI 

       490        500        510        520        530        540 
WVGLWLVIIV VVIVAVEGSF LVKFISRFTQ EIFSILISLI FIYETFSKLG KIFKAHPLVL 

       550        560        570        580        590        600 
NYEHLNDSLD NPFHPVVKEH IEYHEDGNKT VHEVIHERAY PNTALLSMCL MFGCFFIAYF 

       610        620        630        640        650        660 
LRQFKNGHFL PGPIRRMIGD FGVPIAIFFM IAVDITIEDA YTQKLVVPKG LMVSNPNARG 

       670        680        690        700        710        720 
WFINPLGEKK PFPAWMMGAC CVPALLVFIL IFLESQITTL IVSKPERKMV KGSGFHLDLL 

       730        740        750        760        770        780 
ILVTMGGIAS LFGVPWLSAA TVRSVTHANA LTVMSKGPKP EIEKVLEQRI SGMLVAAMVG 

       790        800        810        820        830        840 
VSILLEPILK MIPMTALFGI FLYMGITSLS GIQMWDRMLL LIVPRKYYPA DAYAQRVTTM 

       850        860        870        880        890        900 
KMHLFTLIQM VCLGALWMVK MSAFSLALPF VLILTIPLRM AITGTLFTDK EMKCLDASDG 

       910 
KVKFEEEPGE DMYESPLP 

« Hide

References

[1]"Amino acid sequence of band-3 protein from rainbow trout erythrocytes derived from cDNA."
Hubner S., Michel F., Rudloff V., Appelhans H.
Biochem. J. 285:17-23(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X61699 mRNA. Translation: CAA43868.1.
PIRS24318.
RefSeqNP_001118189.1. NM_001124717.1.
UniGeneOmy.38980.

3D structure databases

ProteinModelPortalP32847.
SMRP32847. Positions 378-419, 812-844.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100136769.

Phylogenomic databases

HOVERGENHBG004326.

Family and domain databases

Gene3D3.40.1100.10. 2 hits.
InterProIPR001717. Anion_exchange.
IPR002977. Anion_exchange_1.
IPR018241. Anion_exchange_CS.
IPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR016152. PTrfase/Anion_transptr.
[Graphical view]
PANTHERPTHR11453. PTHR11453. 1 hit.
PfamPF07565. Band_3_cyto. 2 hits.
PF00955. HCO3_cotransp. 1 hit.
[Graphical view]
PRINTSPR00165. ANIONEXCHNGR.
PR01187. ANIONEXHNGR1.
PR01231. HCO3TRNSPORT.
SUPFAMSSF55804. SSF55804. 1 hit.
TIGRFAMsTIGR00834. ae. 1 hit.
PROSITEPS00219. ANION_EXCHANGER_1. 1 hit.
PS00220. ANION_EXCHANGER_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB3AT_ONCMY
AccessionPrimary (citable) accession number: P32847
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1996
Last modified: July 9, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families