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Protein

Band 3 anion exchange protein

Gene

slc4a1

Organism
Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Anion exchange, Ion transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Band 3 anion exchange protein
Alternative name(s):
Anion exchange protein 1
Short name:
AE 1
Short name:
Anion exchanger 1
Solute carrier family 4 member 1
Gene namesi
Name:slc4a1
Synonyms:ae1
OrganismiOncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Taxonomic identifieri8022 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiProtacanthopterygiiSalmoniformesSalmonidaeSalmoninaeOncorhynchus

Subcellular locationi

  • Cell membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 392392CytoplasmicBy similarityAdd
BLAST
Transmembranei393 – 41624Helical; Name=1By similarityAdd
BLAST
Topological domaini417 – 4248ExtracellularBy similarity
Transmembranei425 – 44521Helical; Name=2By similarityAdd
BLAST
Topological domaini446 – 4483CytoplasmicBy similarity
Transmembranei449 – 46517Discontinuously helical; Name=3By similarityAdd
BLAST
Topological domaini466 – 4749ExtracellularBy similarity
Transmembranei475 – 49521Helical; Name=4By similarityAdd
BLAST
Topological domaini496 – 50712CytoplasmicBy similarityAdd
BLAST
Transmembranei508 – 53023Helical; Name=5By similarityAdd
BLAST
Topological domaini531 – 58353ExtracellularBy similarityAdd
BLAST
Transmembranei584 – 60421Helical; Name=6By similarityAdd
BLAST
Topological domaini605 – 61511CytoplasmicBy similarityAdd
BLAST
Transmembranei616 – 63621Helical; Name=7By similarityAdd
BLAST
Topological domaini637 – 67640ExtracellularBy similarityAdd
BLAST
Transmembranei677 – 69721Helical; Name=8By similarityAdd
BLAST
Topological domaini698 – 71316CytoplasmicBy similarityAdd
BLAST
Transmembranei714 – 73219Helical; Name=9By similarityAdd
BLAST
Transmembranei733 – 75018Discontinuously helical; Name=10By similarityAdd
BLAST
Topological domaini751 – 76919CytoplasmicBy similarityAdd
BLAST
Transmembranei770 – 79021Helical; Name=11By similarityAdd
BLAST
Transmembranei791 – 80919Helical; Name=12By similarityAdd
BLAST
Topological domaini810 – 84738CytoplasmicBy similarityAdd
BLAST
Intramembranei848 – 87831Discontinuously helicalBy similarityAdd
BLAST
Topological domaini879 – 91840CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 918918Band 3 anion exchange proteinPRO_0000079213Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi546 – 5461N-linked (GlcNAc...)Sequence analysis
Glycosylationi568 – 5681N-linked (GlcNAc...)Sequence analysis
Lipidationi852 – 8521S-palmitoyl cysteineBy similarity

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate

Interactioni

Subunit structurei

A dimer in solution, it spans the membrane asymmetrically and appears to be tetrameric.By similarity

Structurei

3D structure databases

ProteinModelPortaliP32847.
SMRiP32847. Positions 378-419, 812-844.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG004326.

Family and domain databases

Gene3Di3.40.1100.10. 2 hits.
InterProiIPR001717. Anion_exchange.
IPR002977. Anion_exchange_1.
IPR018241. Anion_exchange_CS.
IPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR016152. PTrfase/Anion_transptr.
[Graphical view]
PANTHERiPTHR11453. PTHR11453. 1 hit.
PfamiPF07565. Band_3_cyto. 2 hits.
PF00955. HCO3_cotransp. 1 hit.
[Graphical view]
PRINTSiPR00165. ANIONEXCHNGR.
PR01187. ANIONEXHNGR1.
PR01231. HCO3TRNSPORT.
SUPFAMiSSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR00834. ae. 1 hit.
PROSITEiPS00219. ANION_EXCHANGER_1. 1 hit.
PS00220. ANION_EXCHANGER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32847-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENDLSFGED VMSYEEESDS AFPSPIRPTP PGHSGNYDLE QSRQEEDSNQ
60 70 80 90 100
AIQSIVVHTD PEAYLNLNTN ANTRGDAQAY VELNELMGNS WQETGRWVGY
110 120 130 140 150
EENFNPGTGK WGPSHVSYLT FKSLIQLRKI MSTGAIILDL QASSLSAVAE
160 170 180 190 200
KVVDELRTKG EIRAADRDGL LRALLQRRSQ SEGAVAQPLG GDIEMQTFSV
210 220 230 240 250
TKQRDTTDSV EASIVLSGVM DSLEKPAVAF VRLGDSVVIE GALEAPVPVR
260 270 280 290 300
FVFVLVGPSQ GGVDYHESGR AMAALMADWV FSLEAYLAPT NKELTNAIAD
310 320 330 340 350
FMDCGIVIPP TEIQDEGMLQ PIIDFQKKML KDRLRPSDTR IIFGGGAKAD
360 370 380 390 400
EADEEPREDP LARTGIPFGG MIKDMKRRYR HYISDFTDAL DPQVLAAVIF
410 420 430 440 450
IYFAALSPAI TFGGLLADKT EHMMGVSELM ISTCVQGIIF AFIAAQPTLV
460 470 480 490 500
IGFSGPLLVF EEAFFAFCKS QEIEYIVGRI WVGLWLVIIV VVIVAVEGSF
510 520 530 540 550
LVKFISRFTQ EIFSILISLI FIYETFSKLG KIFKAHPLVL NYEHLNDSLD
560 570 580 590 600
NPFHPVVKEH IEYHEDGNKT VHEVIHERAY PNTALLSMCL MFGCFFIAYF
610 620 630 640 650
LRQFKNGHFL PGPIRRMIGD FGVPIAIFFM IAVDITIEDA YTQKLVVPKG
660 670 680 690 700
LMVSNPNARG WFINPLGEKK PFPAWMMGAC CVPALLVFIL IFLESQITTL
710 720 730 740 750
IVSKPERKMV KGSGFHLDLL ILVTMGGIAS LFGVPWLSAA TVRSVTHANA
760 770 780 790 800
LTVMSKGPKP EIEKVLEQRI SGMLVAAMVG VSILLEPILK MIPMTALFGI
810 820 830 840 850
FLYMGITSLS GIQMWDRMLL LIVPRKYYPA DAYAQRVTTM KMHLFTLIQM
860 870 880 890 900
VCLGALWMVK MSAFSLALPF VLILTIPLRM AITGTLFTDK EMKCLDASDG
910
KVKFEEEPGE DMYESPLP
Length:918
Mass (Da):101,894
Last modified:February 1, 1996 - v2
Checksum:i37E163141FBDC16A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61699 mRNA. Translation: CAA43868.1.
PIRiS24318.
RefSeqiNP_001118189.1. NM_001124717.1.
UniGeneiOmy.38980.

Genome annotation databases

GeneIDi100136769.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61699 mRNA. Translation: CAA43868.1.
PIRiS24318.
RefSeqiNP_001118189.1. NM_001124717.1.
UniGeneiOmy.38980.

3D structure databases

ProteinModelPortaliP32847.
SMRiP32847. Positions 378-419, 812-844.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100136769.

Phylogenomic databases

HOVERGENiHBG004326.

Family and domain databases

Gene3Di3.40.1100.10. 2 hits.
InterProiIPR001717. Anion_exchange.
IPR002977. Anion_exchange_1.
IPR018241. Anion_exchange_CS.
IPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR016152. PTrfase/Anion_transptr.
[Graphical view]
PANTHERiPTHR11453. PTHR11453. 1 hit.
PfamiPF07565. Band_3_cyto. 2 hits.
PF00955. HCO3_cotransp. 1 hit.
[Graphical view]
PRINTSiPR00165. ANIONEXCHNGR.
PR01187. ANIONEXHNGR1.
PR01231. HCO3TRNSPORT.
SUPFAMiSSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR00834. ae. 1 hit.
PROSITEiPS00219. ANION_EXCHANGER_1. 1 hit.
PS00220. ANION_EXCHANGER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Amino acid sequence of band-3 protein from rainbow trout erythrocytes derived from cDNA."
    Hubner S., Michel F., Rudloff V., Appelhans H.
    Biochem. J. 285:17-23(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiB3AT_ONCMY
AccessioniPrimary (citable) accession number: P32847
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1996
Last modified: July 6, 2016
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.