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P32841 (RE114_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Meiotic recombination protein REC114
Gene names
Name:REC114
Ordered Locus Names:YMR133W
ORF Names:YM9375.02
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length428 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for the production of double-strand breaks (DSBs) in meiotic recombination initiation; not required for mitosis. Plays a role in activation of the DSB nuclease SPO11 at recombination hotspots. Prevents accumulation of meiotic double strand break intermediates and is needed for the proper timing of the first meiotic division. Component of the MER2-MEI4-REC114 complex which seems to be required for meiotic double-strand break (DSB) formation. Ref.5 Ref.6 Ref.8 Ref.9

Subunit structure

Interacts with MEI4, MER2 and REC104. Component of the MER2-MEI4-REC114 complex. Ref.7 Ref.9

Subcellular location

Nucleus. Chromosome. Note: Localizes to chromosomes. Ref.9

Developmental stage

Meiosis-specific.

Sequence similarities

Belongs to the REC114 family.

Sequence caution

The sequence CAA87347.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 428428Meiotic recombination protein REC114
PRO_0000097219

Experimental info

Sequence conflict96 – 972KY → N Ref.1
Sequence conflict100 – 1034EEFG → RRIR Ref.1
Sequence conflict3771S → C Ref.1

Sequences

Sequence LengthMass (Da)Tools
P32841 [UniParc].

Last modified October 25, 2005. Version 3.
Checksum: 57CB835DA71FBA9B

FASTA42848,706
        10         20         30         40         50         60 
MYEYCSVVIK KYSKYTIPSF APNGFQSMLE PPQIDKWQHL SANCTLQFRV LLMDSRQILI 

        70         80         90        100        110        120 
NVVLNNSTLL ENIRLPLGDN QDLIQFSCKS PIISCKYISE EFGPRMLRRF QMNLPNDVEF 

       130        140        150        160        170        180 
NRTVVSLKNL NFVLRTARTS IAQSTITSQV QGNNNGTKVC FTEGPKVSSY TNPNTQFQTQ 

       190        200        210        220        230        240 
NMIMDFSQRY QEESERESNN RSNITLPHDS IQIAQQIWPN TDLNVVQSSQ DLNTPMATQT 

       250        260        270        280        290        300 
VLGRPESLIV QPLEVSQSPP NTTNCLPNAE NKKKKVDTTS DFTSRKEIAL CKTGLLETIH 

       310        320        330        340        350        360 
IPKERESQMQ SVTGLDATPT IIWSPGKDNT AKKNTSNKKN IDDKLTNPQK SGNTHTPDRN 

       370        380        390        400        410        420 
KEVLPNGTLN ETRKEASPSE GLTIRVKNVN RNASRKISKR LIKEKLKDEE FMKWVNKVET 


VLNKMFEK 

« Hide

References

« Hide 'large scale' references
[1]"Genetic and molecular analysis of REC114, an early meiotic recombination gene in yeast."
Pittman D.L., Lu W., Malone R.E.
Curr. Genet. 23:295-304(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Examination of the intron in the meiosis-specific recombination gene REC114 in Saccharomyces."
Malone R.E., Pittman D.L., Nau J.J.
Mol. Gen. Genet. 255:410-419(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: REVISION OF GENE MODEL.
[5]"Recombination and the progression of meiosis in Saccharomyces cerevisiae."
Galbraith A.M., Bullard S.A., Jiao K., Nau J.J., Malone R.E.
Genetics 146:481-489(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"High copy number suppression of the meiotic arrest caused by a dmc1 mutation: REC114 imposes an early recombination block and RAD54 promotes a DMC1-independent DSB repair pathway."
Bishop D.K., Nikolski Y., Oshiro J., Chon J., Shinohara M., Chen X.
Genes Cells 4:425-444(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Antiviral protein Ski8 is a direct partner of Spo11 in meiotic DNA break formation, independent of its cytoplasmic role in RNA metabolism."
Arora C., Kee K., Maleki S., Keeney S.
Mol. Cell 13:549-559(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MEI4; MER2 AND REC104.
[8]"The control of Spo11's interaction with meiotic recombination hotspots."
Prieler S., Penkner A., Borde V., Klein F.
Genes Dev. 19:255-269(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"Saccharomyces cerevisiae Mer2, Mei4 and Rec114 form a complex required for meiotic double-strand break formation."
Li J., Hooker G.W., Roeder G.S.
Genetics 173:1969-1981(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE MER2-MEI4-REC114 COMPLEX, FUNCTION OF THE MER2-MEI4-REC114 COMPLEX, INTERACTION WITH MEI4 AND MER2, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z14315 Genomic DNA. No translation available.
Z47071 Genomic DNA. Translation: CAA87347.1. Sequence problems.
BK006946 Genomic DNA. Translation: DAA10030.1.
PIRS27453.
RefSeqNP_013852.1. NM_001182634.1.

3D structure databases

ProteinModelPortalP32841.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35310. 15 interactions.
DIPDIP-4006N.
IntActP32841. 3 interactions.
MINTMINT-554399.
STRING4932.YMR133W.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR133W; YMR133W; YMR133W.
GeneID855163.
KEGGsce:YMR133W.

Organism-specific databases

CYGDYMR133w.
SGDS000004740. REC114.

Phylogenomic databases

eggNOGNOG86017.
OMAIPKERES.
OrthoDBEOG7V4BB2.

Enzyme and pathway databases

BioCycYEAST:G3O-32826-MONOMER.

Gene expression databases

GenevestigatorP32841.

Family and domain databases

InterProIPR004354. Meiotic_Rec114.
[Graphical view]
PfamPF03525. Meiotic_rec114. 1 hit.
[Graphical view]
PRINTSPR01548. MEIOTICR114.
ProtoNetSearch...

Other

NextBio978590.

Entry information

Entry nameRE114_YEAST
AccessionPrimary (citable) accession number: P32841
Secondary accession number(s): D6VZV6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 25, 2005
Last modified: July 9, 2014
This is version 99 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families