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P32841

- RE114_YEAST

UniProt

P32841 - RE114_YEAST

Protein

Meiotic recombination protein REC114

Gene

REC114

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 3 (25 Oct 2005)
      Previous versions | rss
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    Functioni

    Required for the production of double-strand breaks (DSBs) in meiotic recombination initiation; not required for mitosis. Plays a role in activation of the DSB nuclease SPO11 at recombination hotspots. Prevents accumulation of meiotic double strand break intermediates and is needed for the proper timing of the first meiotic division. Component of the MER2-MEI4-REC114 complex which seems to be required for meiotic double-strand break (DSB) formation.4 Publications

    GO - Biological processi

    1. meiotic DNA double-strand break formation Source: SGD
    2. reciprocal meiotic recombination Source: SGD

    Keywords - Biological processi

    DNA recombination, Meiosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32826-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Meiotic recombination protein REC114
    Gene namesi
    Name:REC114
    Ordered Locus Names:YMR133W
    ORF Names:YM9375.02
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR133w.
    SGDiS000004740. REC114.

    Subcellular locationi

    Nucleus 1 Publication. Chromosome 1 Publication
    Note: Localizes to chromosomes.

    GO - Cellular componenti

    1. condensed nuclear chromosome Source: SGD

    Keywords - Cellular componenti

    Chromosome, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 428428Meiotic recombination protein REC114PRO_0000097219Add
    BLAST

    Expressioni

    Developmental stagei

    Meiosis-specific.

    Gene expression databases

    GenevestigatoriP32841.

    Interactioni

    Subunit structurei

    Interacts with MEI4, MER2 and REC104. Component of the MER2-MEI4-REC114 complex.2 Publications

    Protein-protein interaction databases

    BioGridi35310. 15 interactions.
    DIPiDIP-4006N.
    IntActiP32841. 3 interactions.
    MINTiMINT-554399.
    STRINGi4932.YMR133W.

    Structurei

    3D structure databases

    ProteinModelPortaliP32841.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the REC114 family.Curated

    Phylogenomic databases

    eggNOGiNOG86017.
    OMAiIPKERES.
    OrthoDBiEOG7V4BB2.

    Family and domain databases

    InterProiIPR004354. Meiotic_Rec114.
    [Graphical view]
    PfamiPF03525. Meiotic_rec114. 1 hit.
    [Graphical view]
    PRINTSiPR01548. MEIOTICR114.

    Sequencei

    Sequence statusi: Complete.

    P32841-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYEYCSVVIK KYSKYTIPSF APNGFQSMLE PPQIDKWQHL SANCTLQFRV    50
    LLMDSRQILI NVVLNNSTLL ENIRLPLGDN QDLIQFSCKS PIISCKYISE 100
    EFGPRMLRRF QMNLPNDVEF NRTVVSLKNL NFVLRTARTS IAQSTITSQV 150
    QGNNNGTKVC FTEGPKVSSY TNPNTQFQTQ NMIMDFSQRY QEESERESNN 200
    RSNITLPHDS IQIAQQIWPN TDLNVVQSSQ DLNTPMATQT VLGRPESLIV 250
    QPLEVSQSPP NTTNCLPNAE NKKKKVDTTS DFTSRKEIAL CKTGLLETIH 300
    IPKERESQMQ SVTGLDATPT IIWSPGKDNT AKKNTSNKKN IDDKLTNPQK 350
    SGNTHTPDRN KEVLPNGTLN ETRKEASPSE GLTIRVKNVN RNASRKISKR 400
    LIKEKLKDEE FMKWVNKVET VLNKMFEK 428
    Length:428
    Mass (Da):48,706
    Last modified:October 25, 2005 - v3
    Checksum:i57CB835DA71FBA9B
    GO

    Sequence cautioni

    The sequence CAA87347.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti96 – 972KY → N(PubMed:8385581)Curated
    Sequence conflicti100 – 1034EEFG → RRIR(PubMed:8385581)Curated
    Sequence conflicti377 – 3771S → C(PubMed:8385581)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z14315 Genomic DNA. No translation available.
    Z47071 Genomic DNA. Translation: CAA87347.1. Sequence problems.
    BK006946 Genomic DNA. Translation: DAA10030.1.
    PIRiS27453.
    RefSeqiNP_013852.1. NM_001182634.1.

    Genome annotation databases

    EnsemblFungiiYMR133W; YMR133W; YMR133W.
    GeneIDi855163.
    KEGGisce:YMR133W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z14315 Genomic DNA. No translation available.
    Z47071 Genomic DNA. Translation: CAA87347.1 . Sequence problems.
    BK006946 Genomic DNA. Translation: DAA10030.1 .
    PIRi S27453.
    RefSeqi NP_013852.1. NM_001182634.1.

    3D structure databases

    ProteinModelPortali P32841.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35310. 15 interactions.
    DIPi DIP-4006N.
    IntActi P32841. 3 interactions.
    MINTi MINT-554399.
    STRINGi 4932.YMR133W.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR133W ; YMR133W ; YMR133W .
    GeneIDi 855163.
    KEGGi sce:YMR133W.

    Organism-specific databases

    CYGDi YMR133w.
    SGDi S000004740. REC114.

    Phylogenomic databases

    eggNOGi NOG86017.
    OMAi IPKERES.
    OrthoDBi EOG7V4BB2.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32826-MONOMER.

    Miscellaneous databases

    NextBioi 978590.

    Gene expression databases

    Genevestigatori P32841.

    Family and domain databases

    InterProi IPR004354. Meiotic_Rec114.
    [Graphical view ]
    Pfami PF03525. Meiotic_rec114. 1 hit.
    [Graphical view ]
    PRINTSi PR01548. MEIOTICR114.
    ProtoNeti Search...

    Publicationsi

    1. "Genetic and molecular analysis of REC114, an early meiotic recombination gene in yeast."
      Pittman D.L., Lu W., Malone R.E.
      Curr. Genet. 23:295-304(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Examination of the intron in the meiosis-specific recombination gene REC114 in Saccharomyces."
      Malone R.E., Pittman D.L., Nau J.J.
      Mol. Gen. Genet. 255:410-419(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVISION OF GENE MODEL.
    5. "Recombination and the progression of meiosis in Saccharomyces cerevisiae."
      Galbraith A.M., Bullard S.A., Jiao K., Nau J.J., Malone R.E.
      Genetics 146:481-489(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "High copy number suppression of the meiotic arrest caused by a dmc1 mutation: REC114 imposes an early recombination block and RAD54 promotes a DMC1-independent DSB repair pathway."
      Bishop D.K., Nikolski Y., Oshiro J., Chon J., Shinohara M., Chen X.
      Genes Cells 4:425-444(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Antiviral protein Ski8 is a direct partner of Spo11 in meiotic DNA break formation, independent of its cytoplasmic role in RNA metabolism."
      Arora C., Kee K., Maleki S., Keeney S.
      Mol. Cell 13:549-559(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MEI4; MER2 AND REC104.
    8. "The control of Spo11's interaction with meiotic recombination hotspots."
      Prieler S., Penkner A., Borde V., Klein F.
      Genes Dev. 19:255-269(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Saccharomyces cerevisiae Mer2, Mei4 and Rec114 form a complex required for meiotic double-strand break formation."
      Li J., Hooker G.W., Roeder G.S.
      Genetics 173:1969-1981(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE MER2-MEI4-REC114 COMPLEX, FUNCTION OF THE MER2-MEI4-REC114 COMPLEX, INTERACTION WITH MEI4 AND MER2, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiRE114_YEAST
    AccessioniPrimary (citable) accession number: P32841
    Secondary accession number(s): D6VZV6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 25, 2005
    Last modified: October 1, 2014
    This is version 100 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3