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Protein

Meiotic recombination protein REC114

Gene

REC114

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for the production of double-strand breaks (DSBs) in meiotic recombination initiation; not required for mitosis. Plays a role in activation of the DSB nuclease SPO11 at recombination hotspots. Prevents accumulation of meiotic double strand break intermediates and is needed for the proper timing of the first meiotic division. Component of the MER2-MEI4-REC114 complex which seems to be required for meiotic double-strand break (DSB) formation.4 Publications

GO - Biological processi

  • meiotic DNA double-strand break formation Source: SGD
  • reciprocal meiotic recombination Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA recombination, Meiosis

Enzyme and pathway databases

BioCyciYEAST:G3O-32826-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Meiotic recombination protein REC114
Gene namesi
Name:REC114
Ordered Locus Names:YMR133W
ORF Names:YM9375.02
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XIII

Organism-specific databases

CYGDiYMR133w.
EuPathDBiFungiDB:YMR133W.
SGDiS000004740. REC114.

Subcellular locationi

  • Nucleus 1 Publication
  • Chromosome 1 Publication

  • Note: Localizes to chromosomes.

GO - Cellular componenti

  • condensed nuclear chromosome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 428428Meiotic recombination protein REC114PRO_0000097219Add
BLAST

Expressioni

Developmental stagei

Meiosis-specific.

Interactioni

Subunit structurei

Interacts with MEI4, MER2 and REC104. Component of the MER2-MEI4-REC114 complex.2 Publications

Protein-protein interaction databases

BioGridi35310. 15 interactions.
DIPiDIP-4006N.
IntActiP32841. 3 interactions.
MINTiMINT-554399.

Structurei

3D structure databases

ProteinModelPortaliP32841.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the REC114 family.Curated

Phylogenomic databases

eggNOGiNOG86017.
InParanoidiP32841.
OrthoDBiEOG7V4BB2.

Family and domain databases

InterProiIPR004354. Meiotic_Rec114.
[Graphical view]
PfamiPF03525. Meiotic_rec114. 1 hit.
[Graphical view]
PRINTSiPR01548. MEIOTICR114.

Sequencei

Sequence statusi: Complete.

P32841-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYEYCSVVIK KYSKYTIPSF APNGFQSMLE PPQIDKWQHL SANCTLQFRV
60 70 80 90 100
LLMDSRQILI NVVLNNSTLL ENIRLPLGDN QDLIQFSCKS PIISCKYISE
110 120 130 140 150
EFGPRMLRRF QMNLPNDVEF NRTVVSLKNL NFVLRTARTS IAQSTITSQV
160 170 180 190 200
QGNNNGTKVC FTEGPKVSSY TNPNTQFQTQ NMIMDFSQRY QEESERESNN
210 220 230 240 250
RSNITLPHDS IQIAQQIWPN TDLNVVQSSQ DLNTPMATQT VLGRPESLIV
260 270 280 290 300
QPLEVSQSPP NTTNCLPNAE NKKKKVDTTS DFTSRKEIAL CKTGLLETIH
310 320 330 340 350
IPKERESQMQ SVTGLDATPT IIWSPGKDNT AKKNTSNKKN IDDKLTNPQK
360 370 380 390 400
SGNTHTPDRN KEVLPNGTLN ETRKEASPSE GLTIRVKNVN RNASRKISKR
410 420
LIKEKLKDEE FMKWVNKVET VLNKMFEK
Length:428
Mass (Da):48,706
Last modified:October 25, 2005 - v3
Checksum:i57CB835DA71FBA9B
GO

Sequence cautioni

The sequence CAA87347.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti96 – 972KY → N (PubMed:8385581).Curated
Sequence conflicti100 – 1034EEFG → RRIR (PubMed:8385581).Curated
Sequence conflicti377 – 3771S → C (PubMed:8385581).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14315 Genomic DNA. No translation available.
Z47071 Genomic DNA. Translation: CAA87347.1. Sequence problems.
BK006946 Genomic DNA. Translation: DAA10030.1.
PIRiS27453.
RefSeqiNP_013852.1. NM_001182634.1.

Genome annotation databases

EnsemblFungiiYMR133W; YMR133W; YMR133W.
GeneIDi855163.
KEGGisce:YMR133W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14315 Genomic DNA. No translation available.
Z47071 Genomic DNA. Translation: CAA87347.1. Sequence problems.
BK006946 Genomic DNA. Translation: DAA10030.1.
PIRiS27453.
RefSeqiNP_013852.1. NM_001182634.1.

3D structure databases

ProteinModelPortaliP32841.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35310. 15 interactions.
DIPiDIP-4006N.
IntActiP32841. 3 interactions.
MINTiMINT-554399.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR133W; YMR133W; YMR133W.
GeneIDi855163.
KEGGisce:YMR133W.

Organism-specific databases

CYGDiYMR133w.
EuPathDBiFungiDB:YMR133W.
SGDiS000004740. REC114.

Phylogenomic databases

eggNOGiNOG86017.
InParanoidiP32841.
OrthoDBiEOG7V4BB2.

Enzyme and pathway databases

BioCyciYEAST:G3O-32826-MONOMER.

Miscellaneous databases

NextBioi978590.
PROiP32841.

Family and domain databases

InterProiIPR004354. Meiotic_Rec114.
[Graphical view]
PfamiPF03525. Meiotic_rec114. 1 hit.
[Graphical view]
PRINTSiPR01548. MEIOTICR114.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genetic and molecular analysis of REC114, an early meiotic recombination gene in yeast."
    Pittman D.L., Lu W., Malone R.E.
    Curr. Genet. 23:295-304(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Examination of the intron in the meiosis-specific recombination gene REC114 in Saccharomyces."
    Malone R.E., Pittman D.L., Nau J.J.
    Mol. Gen. Genet. 255:410-419(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVISION OF GENE MODEL.
  5. "Recombination and the progression of meiosis in Saccharomyces cerevisiae."
    Galbraith A.M., Bullard S.A., Jiao K., Nau J.J., Malone R.E.
    Genetics 146:481-489(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "High copy number suppression of the meiotic arrest caused by a dmc1 mutation: REC114 imposes an early recombination block and RAD54 promotes a DMC1-independent DSB repair pathway."
    Bishop D.K., Nikolski Y., Oshiro J., Chon J., Shinohara M., Chen X.
    Genes Cells 4:425-444(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Antiviral protein Ski8 is a direct partner of Spo11 in meiotic DNA break formation, independent of its cytoplasmic role in RNA metabolism."
    Arora C., Kee K., Maleki S., Keeney S.
    Mol. Cell 13:549-559(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MEI4; MER2 AND REC104.
  8. "The control of Spo11's interaction with meiotic recombination hotspots."
    Prieler S., Penkner A., Borde V., Klein F.
    Genes Dev. 19:255-269(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Saccharomyces cerevisiae Mer2, Mei4 and Rec114 form a complex required for meiotic double-strand break formation."
    Li J., Hooker G.W., Roeder G.S.
    Genetics 173:1969-1981(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE MER2-MEI4-REC114 COMPLEX, FUNCTION OF THE MER2-MEI4-REC114 COMPLEX, INTERACTION WITH MEI4 AND MER2, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiRE114_YEAST
AccessioniPrimary (citable) accession number: P32841
Secondary accession number(s): D6VZV6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 25, 2005
Last modified: July 22, 2015
This is version 105 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.