P32839 (BCS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial chaperone BCS1

Gene names

Name:	BCS1
Ordered Locus Names:	YDR375C
ORF Names:	D9481.17

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	456 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for the expression of the Rieske iron-sulfur protein.
Subcellular location	Mitochondrion inner membrane; Single-pass membrane protein.
Miscellaneous	Present with 1990 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the AAA ATPase family. BCS1 subfamily.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aerobic respiration Inferred from mutant phenotype Ref.1. Source: SGD chaperone-mediated protein complex assembly Inferred from mutant phenotype. Source: SGD mitochondrion organization Inferred from mutant phenotype. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from direct assay Ref.4. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 456	456	Mitochondrial chaperone BCS1		PRO_0000084777
Regions
	Topological domain	1 – 44	44	Mitochondrial intermembrane Probable
	Transmembrane	45 – 68	24	Helical; Probable
	Topological domain	69 – 456	388	Mitochondrial matrix Probable
	Nucleotide binding	267 – 274	8	ATP Potential
	Motif	69 – 83	15	Mitochondrial-targeting signal
Amino acid modifications
	Modified residue	227	1	Phosphoserine Ref.6
Experimental info
	Sequence conflict	119	1	N → F in AAC09007. Ref.1
	Sequence conflict	267	1	G → S in AAC09007. Ref.1
	Sequence conflict	349	1	L → Q in AAC09007. Ref.1
	Sequence conflict	351	1	G → S in AAC09007. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P32839 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 17750D26B664ED04	FASTA	456	51,107
	10 20 30 40 50 60 MSDKPIDIQY DKQATPNLSG VITPPTNETG NDSVREKLSK LVGDAMSNNP YFAAGGGLMI 70 80 90 100 110 120 LGTGLAVARS GIIKASRVLY RQMIVDLEIQ SKDKSYAWFL TWMAKHPQRV SRHLSVRTNY 130 140 150 160 170 180 IQHDNGSVST KFSLVPGPGN HWIRYKGAFI LIKRERSAKM IDIANGSPFE TVTLTTLYRD 190 200 210 220 230 240 KHLFDDILNE AKDIALKTTE GKTVIYTSFG PEWRKFGQPK AKRMLPSVIL DSGIKEGILD 250 260 270 280 290 300 DVYDFMKNGK WYSDRGIPYR RGYLLYGPPG SGKTSFIQAL AGELDYNICI LNLSENNLTD 310 320 330 340 350 360 DRLNHLMNNM PERSILLLED IDAAFNKRSQ TGEQGFHSSV TFSGLLNALD GVTSSEETIT 370 380 390 400 410 420 FMTTNHPEKL DAAIMRPGRI DYKVFVGNAT PYQVEKMFMK FYPGETDICK KFVNSVKELD 430 440 450 ITVSTAQLQG LFVMNKDAPH DALKMVSSLR NANHIF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"BCS1, a novel gene required for the expression of functional Rieske iron-sulfur protein in Saccharomyces cerevisiae." Nobrega F.G., Nobrega M.P., Tzagoloff A. EMBO J. 11:3821-3829(1992) [PubMed: 1327750] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Internal targeting signal of the BCS1 protein: a novel mechanism of import into mitochondria." Foelsch H., Guiard B., Neupert W., Stuart R.A. EMBO J. 15:479-487(1996) [PubMed: 8599931] [Abstract] Cited for: TOPOLOGY.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-227, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S47190 Genomic DNA. Translation: AAC09007.1. U28373 Genomic DNA. Translation: AAB64811.1. BK006938 Genomic DNA. Translation: DAA12217.1.
PIR	S61170.
RefSeq	NP_010663.1. NM_001180683.1.
3D structure databases
ProteinModelPortal	P32839.
SMR	P32839. Positions 221-433.
ModBase	Search...
Protein-protein interaction databases
IntAct	P32839. 1 interaction.
STRING	P32839.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR375C; YDR375C; YDR375C.
GeneID	851981.
KEGG	sce:YDR375C.
NMPDR	fig|4932.3.peg.1433.
Organism-specific databases
CYGD	YDR375c.
SGD	S000002783. BCS1.
Phylogenomic databases
eggNOG	fuNOG06383.
GeneTree	EFGT00050000002024.
HOGENOM	HBG521794.
OMA	PSKDKSY.
OrthoDB	EOG4XWK6J.
Gene expression databases
ArrayExpress	P32839.
Genevestigator	P32839.
GermOnline	YDR375C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. IPR014851. BCS1_N. [Graphical view]
KO	K08900.
Pfam	PF00004. AAA. 1 hit. PF08740. BCS1_N. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. SM01024. BCS1_N. 1 hit. [Graphical view]
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	970127.

Entry information

Entry name

BCS1_YEAST

Accession

Primary (citable) accession number: P32839
Secondary accession number(s): D6VT07, Q06404

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program