Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P32838

- PP2A4_YEAST

UniProt

P32838 - PP2A4_YEAST

Protein

Serine/threonine-protein phosphatase PP2A-like PPG1

Gene

PPG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in glycogen accumulation.

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi50 – 501Manganese 1By similarity
    Metal bindingi52 – 521Manganese 1By similarity
    Metal bindingi78 – 781Manganese 1By similarity
    Metal bindingi78 – 781Manganese 2By similarity
    Metal bindingi110 – 1101Manganese 2By similarity
    Active sitei111 – 1111Proton donorBy similarity
    Metal bindingi161 – 1611Manganese 2By similarity
    Metal bindingi247 – 2471Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein serine/threonine phosphatase activity Source: SGD

    GO - Biological processi

    1. glycogen metabolic process Source: SGD
    2. protein dephosphorylation Source: SGD

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33343-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase PP2A-like PPG1 (EC:3.1.3.16)
    Gene namesi
    Name:PPG1
    Synonyms:PPG
    Ordered Locus Names:YNR032W
    ORF Names:N3281
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNR032w.
    SGDiS000005315. PPG1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 368368Serine/threonine-protein phosphatase PP2A-like PPG1PRO_0000058876Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei368 – 3681Leucine methyl esterBy similarity

    Post-translational modificationi

    Reversibly methyl esterified on Leu-368 by leucine carboxyl methyltransferase 1 (PPM1) and protein phosphatase methylesterase 1 (PPE1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization.

    Keywords - PTMi

    Methylation

    Proteomic databases

    MaxQBiP32838.
    PaxDbiP32838.

    Expressioni

    Gene expression databases

    GenevestigatoriP32838.

    Interactioni

    Subunit structurei

    Inactivated in a complex with phosphatase methylesterase PPE1 (PP2Ai). Interacts with phosphatase 2A activator RRD1, which can reactivate PP2Ai by dissociating the catalytic subunit from the complex. Interacts with TAP42.2 Publications

    Protein-protein interaction databases

    BioGridi35856. 98 interactions.
    DIPiDIP-1523N.
    IntActiP32838. 10 interactions.
    MINTiMINT-393078.
    STRINGi4932.YNR032W.

    Structurei

    3D structure databases

    ProteinModelPortaliP32838.
    SMRiP32838. Positions 2-307.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-2A subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0639.
    GeneTreeiENSGT00710000107291.
    HOGENOMiHOG000172696.
    KOiK17615.
    OMAiFYTECVR.
    OrthoDBiEOG7FFN29.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32838-1 [UniParc]FASTAAdd to Basket

    « Hide

    MELDECLERL YKAQLLPEVT VRALCFKLKE MLVKESNVIH IQTPVTVVGD    50
    MHGQFHDMLE IFQIGGPVPD TNYLFLGDYV DRGLYSVETI MLLIVLKLRY 100
    PSRIHLLRGN HESRQITQSY GFYTECLNKY GGNSRVWQYL TDIFDYLVLC 150
    CIIDDEIFCV HGGLSPNVQT IDQIKIIDRF REIPHDGAMA DLVWSDPEEN 200
    NNPTLDHPDN SGQHFQVSPR GAGYTFGRSV VEKFLRMNDM NRIYRAHQLC 250
    NEGYQIYFDG LVTTVWSAPN YCYRCGNKAS ILELYSKDQF YFNVFEEAPE 300
    NKLLKENSMN DNALEDSISN PVANRKLIAD YFEDDSASAD GSTDPEMYIF 350
    SDVYQARSAS NRHVDYFL 368
    Length:368
    Mass (Da):42,433
    Last modified:October 1, 1996 - v2
    Checksum:i1B326BBC41A67481
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti126 – 1261C → S in AAA34895. (PubMed:7678255)Curated
    Sequence conflicti154 – 1541D → G in AAS56347. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M94269 Genomic DNA. Translation: AAA34895.1.
    Z71647 Genomic DNA. Translation: CAA96312.1.
    AY558021 Genomic DNA. Translation: AAS56347.1.
    BK006947 Genomic DNA. Translation: DAA10572.1.
    PIRiS63363.
    RefSeqiNP_014429.3. NM_001183209.3.

    Genome annotation databases

    EnsemblFungiiYNR032W; YNR032W; YNR032W.
    GeneIDi855766.
    KEGGisce:YNR032W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M94269 Genomic DNA. Translation: AAA34895.1 .
    Z71647 Genomic DNA. Translation: CAA96312.1 .
    AY558021 Genomic DNA. Translation: AAS56347.1 .
    BK006947 Genomic DNA. Translation: DAA10572.1 .
    PIRi S63363.
    RefSeqi NP_014429.3. NM_001183209.3.

    3D structure databases

    ProteinModelPortali P32838.
    SMRi P32838. Positions 2-307.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35856. 98 interactions.
    DIPi DIP-1523N.
    IntActi P32838. 10 interactions.
    MINTi MINT-393078.
    STRINGi 4932.YNR032W.

    Proteomic databases

    MaxQBi P32838.
    PaxDbi P32838.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNR032W ; YNR032W ; YNR032W .
    GeneIDi 855766.
    KEGGi sce:YNR032W.

    Organism-specific databases

    CYGDi YNR032w.
    SGDi S000005315. PPG1.

    Phylogenomic databases

    eggNOGi COG0639.
    GeneTreei ENSGT00710000107291.
    HOGENOMi HOG000172696.
    KOi K17615.
    OMAi FYTECVR.
    OrthoDBi EOG7FFN29.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33343-MONOMER.

    Miscellaneous databases

    NextBioi 980208.

    Gene expression databases

    Genevestigatori P32838.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The gene PPG encodes a novel yeast protein phosphatase involved in glycogen accumulation."
      Posas F., Clotet J., Muns M.T., Corominas J., Casamayor A., Arino J.
      J. Biol. Chem. 268:1349-1354(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Interaction with Tap42 is required for the essential function of Sit4 and type 2A phosphatases."
      Wang H., Wang X., Jiang Y.
      Mol. Biol. Cell 14:4342-4351(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TAP42.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Specific interactions of PP2A and PP2A-like phosphatases with the yeast PTPA homologues, Ypa1 and Ypa2."
      Van Hoof C., Martens E., Longin S., Jordens J., Stevens I., Janssens V., Goris J.
      Biochem. J. 386:93-102(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PPE1 AND RRD1.

    Entry informationi

    Entry nameiPP2A4_YEAST
    AccessioniPrimary (citable) accession number: P32838
    Secondary accession number(s): D6W1K6, E9P8U5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 130 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1960 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3