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P32838

- PP2A4_YEAST

UniProt

P32838 - PP2A4_YEAST

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Protein

Serine/threonine-protein phosphatase PP2A-like PPG1

Gene

PPG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Involved in glycogen accumulation.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi50 – 501Manganese 1By similarity
Metal bindingi52 – 521Manganese 1By similarity
Metal bindingi78 – 781Manganese 1By similarity
Metal bindingi78 – 781Manganese 2By similarity
Metal bindingi110 – 1101Manganese 2By similarity
Active sitei111 – 1111Proton donorBy similarity
Metal bindingi161 – 1611Manganese 2By similarity
Metal bindingi247 – 2471Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein serine/threonine phosphatase activity Source: SGD

GO - Biological processi

  1. glycogen metabolic process Source: SGD
  2. protein dephosphorylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33343-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP2A-like PPG1 (EC:3.1.3.16)
Gene namesi
Name:PPG1
Synonyms:PPG
Ordered Locus Names:YNR032W
ORF Names:N3281
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNR032w.
SGDiS000005315. PPG1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 368368Serine/threonine-protein phosphatase PP2A-like PPG1PRO_0000058876Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei368 – 3681Leucine methyl esterBy similarity

Post-translational modificationi

Reversibly methyl esterified on Leu-368 by leucine carboxyl methyltransferase 1 (PPM1) and protein phosphatase methylesterase 1 (PPE1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization.

Keywords - PTMi

Methylation

Proteomic databases

MaxQBiP32838.
PaxDbiP32838.

Expressioni

Gene expression databases

GenevestigatoriP32838.

Interactioni

Subunit structurei

Inactivated in a complex with phosphatase methylesterase PPE1 (PP2Ai). Interacts with phosphatase 2A activator RRD1, which can reactivate PP2Ai by dissociating the catalytic subunit from the complex. Interacts with TAP42.2 Publications

Protein-protein interaction databases

BioGridi35856. 99 interactions.
DIPiDIP-1523N.
IntActiP32838. 10 interactions.
MINTiMINT-393078.
STRINGi4932.YNR032W.

Structurei

3D structure databases

ProteinModelPortaliP32838.
SMRiP32838. Positions 2-299.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-2A subfamily.Curated

Phylogenomic databases

eggNOGiCOG0639.
GeneTreeiENSGT00710000107291.
HOGENOMiHOG000172696.
InParanoidiP32838.
KOiK17615.
OMAiFYTECVR.
OrthoDBiEOG7FFN29.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32838-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MELDECLERL YKAQLLPEVT VRALCFKLKE MLVKESNVIH IQTPVTVVGD
60 70 80 90 100
MHGQFHDMLE IFQIGGPVPD TNYLFLGDYV DRGLYSVETI MLLIVLKLRY
110 120 130 140 150
PSRIHLLRGN HESRQITQSY GFYTECLNKY GGNSRVWQYL TDIFDYLVLC
160 170 180 190 200
CIIDDEIFCV HGGLSPNVQT IDQIKIIDRF REIPHDGAMA DLVWSDPEEN
210 220 230 240 250
NNPTLDHPDN SGQHFQVSPR GAGYTFGRSV VEKFLRMNDM NRIYRAHQLC
260 270 280 290 300
NEGYQIYFDG LVTTVWSAPN YCYRCGNKAS ILELYSKDQF YFNVFEEAPE
310 320 330 340 350
NKLLKENSMN DNALEDSISN PVANRKLIAD YFEDDSASAD GSTDPEMYIF
360
SDVYQARSAS NRHVDYFL
Length:368
Mass (Da):42,433
Last modified:October 1, 1996 - v2
Checksum:i1B326BBC41A67481
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti126 – 1261C → S in AAA34895. (PubMed:7678255)Curated
Sequence conflicti154 – 1541D → G in AAS56347. (PubMed:17322287)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M94269 Genomic DNA. Translation: AAA34895.1.
Z71647 Genomic DNA. Translation: CAA96312.1.
AY558021 Genomic DNA. Translation: AAS56347.1.
BK006947 Genomic DNA. Translation: DAA10572.1.
PIRiS63363.
RefSeqiNP_014429.3. NM_001183209.3.

Genome annotation databases

EnsemblFungiiYNR032W; YNR032W; YNR032W.
GeneIDi855766.
KEGGisce:YNR032W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M94269 Genomic DNA. Translation: AAA34895.1 .
Z71647 Genomic DNA. Translation: CAA96312.1 .
AY558021 Genomic DNA. Translation: AAS56347.1 .
BK006947 Genomic DNA. Translation: DAA10572.1 .
PIRi S63363.
RefSeqi NP_014429.3. NM_001183209.3.

3D structure databases

ProteinModelPortali P32838.
SMRi P32838. Positions 2-299.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35856. 99 interactions.
DIPi DIP-1523N.
IntActi P32838. 10 interactions.
MINTi MINT-393078.
STRINGi 4932.YNR032W.

Proteomic databases

MaxQBi P32838.
PaxDbi P32838.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNR032W ; YNR032W ; YNR032W .
GeneIDi 855766.
KEGGi sce:YNR032W.

Organism-specific databases

CYGDi YNR032w.
SGDi S000005315. PPG1.

Phylogenomic databases

eggNOGi COG0639.
GeneTreei ENSGT00710000107291.
HOGENOMi HOG000172696.
InParanoidi P32838.
KOi K17615.
OMAi FYTECVR.
OrthoDBi EOG7FFN29.

Enzyme and pathway databases

BioCyci YEAST:G3O-33343-MONOMER.

Miscellaneous databases

NextBioi 980208.

Gene expression databases

Genevestigatori P32838.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR00114. STPHPHTASE.
SMARTi SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The gene PPG encodes a novel yeast protein phosphatase involved in glycogen accumulation."
    Posas F., Clotet J., Muns M.T., Corominas J., Casamayor A., Arino J.
    J. Biol. Chem. 268:1349-1354(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Interaction with Tap42 is required for the essential function of Sit4 and type 2A phosphatases."
    Wang H., Wang X., Jiang Y.
    Mol. Biol. Cell 14:4342-4351(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TAP42.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Specific interactions of PP2A and PP2A-like phosphatases with the yeast PTPA homologues, Ypa1 and Ypa2."
    Van Hoof C., Martens E., Longin S., Jordens J., Stevens I., Janssens V., Goris J.
    Biochem. J. 386:93-102(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PPE1 AND RRD1.

Entry informationi

Entry nameiPP2A4_YEAST
AccessioniPrimary (citable) accession number: P32838
Secondary accession number(s): D6W1K6, E9P8U5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1960 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3