P32838 (PP2A4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase PP2A-like PPG1
EC=3.1.3.16

Gene names

Name:	PPG1
Synonyms:	PPG
Ordered Locus Names:	YNR032W
ORF Names:	N3281

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in glycogen accumulation.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Subunit structure	Inactivated in a complex with phosphatase methylesterase PPE1 (PP2Ai). Interacts with phosphatase 2A activator RRD1, which can reactivate PP2Ai by dissociating the catalytic subunit from the complex. Interacts with TAP42. Ref.5 Ref.7
Miscellaneous	Present with 1960 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the PPP phosphatase family. PP-2A subfamily.

Ontologies

Keywords
Ligand	Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
PTM	Methylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycogen metabolic process Inferred from mutant phenotype Ref.1. Source: SGD protein dephosphorylation Inferred from sequence or structural similarity Ref.1. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 14562095. Source: SGD nucleus Inferred from direct assay PubMed 14562095. Source: SGD
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from sequence or structural similarity Ref.1. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 368	368	Serine/threonine-protein phosphatase PP2A-like PPG1		PRO_0000058876
Sites
	Active site	111	1	Proton donor By similarity
	Metal binding	50	1	Iron By similarity
	Metal binding	52	1	Iron By similarity
	Metal binding	78	1	Iron By similarity
	Metal binding	78	1	Manganese By similarity
	Metal binding	110	1	Manganese By similarity
	Metal binding	161	1	Manganese By similarity
	Metal binding	247	1	Manganese By similarity
Amino acid modifications
	Modified residue	368	1	Leucine methyl ester By similarity
Experimental info
	Sequence conflict	126	1	C → S in AAA34895. Ref.1
	Sequence conflict	154	1	D → G in AAS56347. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P32838 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 1B326BBC41A67481	FASTA	368	42,433
	10 20 30 40 50 60 MELDECLERL YKAQLLPEVT VRALCFKLKE MLVKESNVIH IQTPVTVVGD MHGQFHDMLE 70 80 90 100 110 120 IFQIGGPVPD TNYLFLGDYV DRGLYSVETI MLLIVLKLRY PSRIHLLRGN HESRQITQSY 130 140 150 160 170 180 GFYTECLNKY GGNSRVWQYL TDIFDYLVLC CIIDDEIFCV HGGLSPNVQT IDQIKIIDRF 190 200 210 220 230 240 REIPHDGAMA DLVWSDPEEN NNPTLDHPDN SGQHFQVSPR GAGYTFGRSV VEKFLRMNDM 250 260 270 280 290 300 NRIYRAHQLC NEGYQIYFDG LVTTVWSAPN YCYRCGNKAS ILELYSKDQF YFNVFEEAPE 310 320 330 340 350 360 NKLLKENSMN DNALEDSISN PVANRKLIAD YFEDDSASAD GSTDPEMYIF SDVYQARSAS NRHVDYFL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The gene PPG encodes a novel yeast protein phosphatase involved in glycogen accumulation." Posas F., Clotet J., Muns M.T., Corominas J., Casamayor A., Arino J. J. Biol. Chem. 268:1349-1354(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Interaction with Tap42 is required for the essential function of Sit4 and type 2A phosphatases." Wang H., Wang X., Jiang Y. Mol. Biol. Cell 14:4342-4351(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TAP42.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Specific interactions of PP2A and PP2A-like phosphatases with the yeast PTPA homologues, Ypa1 and Ypa2." Van Hoof C., Martens E., Longin S., Jordens J., Stevens I., Janssens V., Goris J. Biochem. J. 386:93-102(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PPE1 AND RRD1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M94269 Genomic DNA. Translation: AAA34895.1. Z71647 Genomic DNA. Translation: CAA96312.1. AY558021 Genomic DNA. Translation: AAS56347.1. BK006947 Genomic DNA. Translation: DAA10572.1.
PIR	S63363.
RefSeq	NP_014429.3. NM_001183209.3.
3D structure databases
ProteinModelPortal	P32838.
SMR	P32838. Positions 2-299.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1523N.
IntAct	P32838. 10 interactions.
MINT	MINT-393078.
STRING	4932.YNR032W.
Proteomic databases
PaxDb	P32838.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNR032W; YNR032W; YNR032W.
GeneID	855766.
KEGG	sce:YNR032W. sce:YNR037C.
Organism-specific databases
CYGD	YNR032w.
SGD	S000005315. PPG1.
Phylogenomic databases
eggNOG	COG0639.
GeneTree	ENSGT00700000105882.
HOGENOM	HOG000172696.
KO	K01090.
OMA	FREIPHE.
OrthoDB	EOG4JHGQ8.
Gene expression databases
Genevestigator	P32838.
GermOnline	YNR032W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980208.

Entry information

Entry name

PP2A4_YEAST

Accession

Primary (citable) accession number: P32838
Secondary accession number(s): D6W1K6, E9P8U5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 118 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program