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Protein

Serine/threonine-protein phosphatase PP2A-like PPG1

Gene

PPG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in glycogen accumulation.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi50Manganese 1By similarity1
Metal bindingi52Manganese 1By similarity1
Metal bindingi78Manganese 1By similarity1
Metal bindingi78Manganese 2By similarity1
Metal bindingi110Manganese 2By similarity1
Active sitei111Proton donorBy similarity1
Metal bindingi161Manganese 2By similarity1
Metal bindingi247Manganese 2By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: SGD

GO - Biological processi

  • glycogen metabolic process Source: SGD
  • protein dephosphorylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33343-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP2A-like PPG1 (EC:3.1.3.16)
Gene namesi
Name:PPG1
Synonyms:PPG
Ordered Locus Names:YNR032W
ORF Names:N3281
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNR032W.
SGDiS000005315. PPG1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000588761 – 368Serine/threonine-protein phosphatase PP2A-like PPG1Add BLAST368

Post-translational modificationi

Reversibly methyl esterified on Leu-368 by leucine carboxyl methyltransferase 1 (PPM1) and protein phosphatase methylesterase 1 (PPE1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization.

Keywords - PTMi

Methylation

Proteomic databases

MaxQBiP32838.
PRIDEiP32838.
TopDownProteomicsiP32838.

Interactioni

Subunit structurei

Inactivated in a complex with phosphatase methylesterase PPE1 (PP2Ai). Interacts with phosphatase 2A activator RRD1, which can reactivate PP2Ai by dissociating the catalytic subunit from the complex. Interacts with TAP42.2 Publications

Protein-protein interaction databases

BioGridi35856. 99 interactors.
DIPiDIP-1523N.
IntActiP32838. 10 interactors.
MINTiMINT-393078.

Structurei

3D structure databases

ProteinModelPortaliP32838.
SMRiP32838.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-2A subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00860000135193.
HOGENOMiHOG000172696.
InParanoidiP32838.
KOiK17615.
OMAiFYTECVR.
OrthoDBiEOG092C2R40.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32838-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELDECLERL YKAQLLPEVT VRALCFKLKE MLVKESNVIH IQTPVTVVGD
60 70 80 90 100
MHGQFHDMLE IFQIGGPVPD TNYLFLGDYV DRGLYSVETI MLLIVLKLRY
110 120 130 140 150
PSRIHLLRGN HESRQITQSY GFYTECLNKY GGNSRVWQYL TDIFDYLVLC
160 170 180 190 200
CIIDDEIFCV HGGLSPNVQT IDQIKIIDRF REIPHDGAMA DLVWSDPEEN
210 220 230 240 250
NNPTLDHPDN SGQHFQVSPR GAGYTFGRSV VEKFLRMNDM NRIYRAHQLC
260 270 280 290 300
NEGYQIYFDG LVTTVWSAPN YCYRCGNKAS ILELYSKDQF YFNVFEEAPE
310 320 330 340 350
NKLLKENSMN DNALEDSISN PVANRKLIAD YFEDDSASAD GSTDPEMYIF
360
SDVYQARSAS NRHVDYFL
Length:368
Mass (Da):42,433
Last modified:October 1, 1996 - v2
Checksum:i1B326BBC41A67481
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti126C → S in AAA34895 (PubMed:7678255).Curated1
Sequence conflicti154D → G in AAS56347 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94269 Genomic DNA. Translation: AAA34895.1.
Z71647 Genomic DNA. Translation: CAA96312.1.
AY558021 Genomic DNA. Translation: AAS56347.1.
BK006947 Genomic DNA. Translation: DAA10572.1.
PIRiS63363.
RefSeqiNP_014429.3. NM_001183209.3.

Genome annotation databases

EnsemblFungiiYNR032W; YNR032W; YNR032W.
GeneIDi855766.
KEGGisce:YNR032W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94269 Genomic DNA. Translation: AAA34895.1.
Z71647 Genomic DNA. Translation: CAA96312.1.
AY558021 Genomic DNA. Translation: AAS56347.1.
BK006947 Genomic DNA. Translation: DAA10572.1.
PIRiS63363.
RefSeqiNP_014429.3. NM_001183209.3.

3D structure databases

ProteinModelPortaliP32838.
SMRiP32838.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35856. 99 interactors.
DIPiDIP-1523N.
IntActiP32838. 10 interactors.
MINTiMINT-393078.

Proteomic databases

MaxQBiP32838.
PRIDEiP32838.
TopDownProteomicsiP32838.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNR032W; YNR032W; YNR032W.
GeneIDi855766.
KEGGisce:YNR032W.

Organism-specific databases

EuPathDBiFungiDB:YNR032W.
SGDiS000005315. PPG1.

Phylogenomic databases

GeneTreeiENSGT00860000135193.
HOGENOMiHOG000172696.
InParanoidiP32838.
KOiK17615.
OMAiFYTECVR.
OrthoDBiEOG092C2R40.

Enzyme and pathway databases

BioCyciYEAST:G3O-33343-MONOMER.

Miscellaneous databases

PROiP32838.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPP2A4_YEAST
AccessioniPrimary (citable) accession number: P32838
Secondary accession number(s): D6W1K6, E9P8U5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 152 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1960 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.