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P32838 (PP2A4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase PP2A-like PPG1

EC=3.1.3.16
Gene names
Name:PPG1
Synonyms:PPG
Ordered Locus Names:YNR032W
ORF Names:N3281
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length368 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in glycogen accumulation.

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 manganese ions per subunit By similarity.

Subunit structure

Inactivated in a complex with phosphatase methylesterase PPE1 (PP2Ai). Interacts with phosphatase 2A activator RRD1, which can reactivate PP2Ai by dissociating the catalytic subunit from the complex. Interacts with TAP42. Ref.5 Ref.7

Post-translational modification

Reversibly methyl esterified on Leu-368 by leucine carboxyl methyltransferase 1 (PPM1) and protein phosphatase methylesterase 1 (PPE1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization.

Miscellaneous

Present with 1960 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the PPP phosphatase family. PP-2A subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 368368Serine/threonine-protein phosphatase PP2A-like PPG1
PRO_0000058876

Sites

Active site1111Proton donor By similarity
Metal binding501Manganese 1 By similarity
Metal binding521Manganese 1 By similarity
Metal binding781Manganese 1 By similarity
Metal binding781Manganese 2 By similarity
Metal binding1101Manganese 2 By similarity
Metal binding1611Manganese 2 By similarity
Metal binding2471Manganese 2 By similarity

Amino acid modifications

Modified residue3681Leucine methyl ester By similarity

Experimental info

Sequence conflict1261C → S in AAA34895. Ref.1
Sequence conflict1541D → G in AAS56347. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P32838 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 1B326BBC41A67481

FASTA36842,433
        10         20         30         40         50         60 
MELDECLERL YKAQLLPEVT VRALCFKLKE MLVKESNVIH IQTPVTVVGD MHGQFHDMLE 

        70         80         90        100        110        120 
IFQIGGPVPD TNYLFLGDYV DRGLYSVETI MLLIVLKLRY PSRIHLLRGN HESRQITQSY 

       130        140        150        160        170        180 
GFYTECLNKY GGNSRVWQYL TDIFDYLVLC CIIDDEIFCV HGGLSPNVQT IDQIKIIDRF 

       190        200        210        220        230        240 
REIPHDGAMA DLVWSDPEEN NNPTLDHPDN SGQHFQVSPR GAGYTFGRSV VEKFLRMNDM 

       250        260        270        280        290        300 
NRIYRAHQLC NEGYQIYFDG LVTTVWSAPN YCYRCGNKAS ILELYSKDQF YFNVFEEAPE 

       310        320        330        340        350        360 
NKLLKENSMN DNALEDSISN PVANRKLIAD YFEDDSASAD GSTDPEMYIF SDVYQARSAS 


NRHVDYFL 

« Hide

References

« Hide 'large scale' references
[1]"The gene PPG encodes a novel yeast protein phosphatase involved in glycogen accumulation."
Posas F., Clotet J., Muns M.T., Corominas J., Casamayor A., Arino J.
J. Biol. Chem. 268:1349-1354(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Interaction with Tap42 is required for the essential function of Sit4 and type 2A phosphatases."
Wang H., Wang X., Jiang Y.
Mol. Biol. Cell 14:4342-4351(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TAP42.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Specific interactions of PP2A and PP2A-like phosphatases with the yeast PTPA homologues, Ypa1 and Ypa2."
Van Hoof C., Martens E., Longin S., Jordens J., Stevens I., Janssens V., Goris J.
Biochem. J. 386:93-102(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PPE1 AND RRD1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M94269 Genomic DNA. Translation: AAA34895.1.
Z71647 Genomic DNA. Translation: CAA96312.1.
AY558021 Genomic DNA. Translation: AAS56347.1.
BK006947 Genomic DNA. Translation: DAA10572.1.
PIRS63363.
RefSeqNP_014429.3. NM_001183209.3.

3D structure databases

ProteinModelPortalP32838.
SMRP32838. Positions 2-307.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35856. 98 interactions.
DIPDIP-1523N.
IntActP32838. 10 interactions.
MINTMINT-393078.
STRING4932.YNR032W.

Proteomic databases

MaxQBP32838.
PaxDbP32838.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNR032W; YNR032W; YNR032W.
GeneID855766.
KEGGsce:YNR032W.

Organism-specific databases

CYGDYNR032w.
SGDS000005315. PPG1.

Phylogenomic databases

eggNOGCOG0639.
GeneTreeENSGT00710000107291.
HOGENOMHOG000172696.
KOK17615.
OMAFYTECVR.
OrthoDBEOG7FFN29.

Enzyme and pathway databases

BioCycYEAST:G3O-33343-MONOMER.

Gene expression databases

GenevestigatorP32838.

Family and domain databases

Gene3D3.60.21.10. 1 hit.
InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR00114. STPHPHTASE.
SMARTSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMSSF56300. SSF56300. 1 hit.
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio980208.

Entry information

Entry namePP2A4_YEAST
AccessionPrimary (citable) accession number: P32838
Secondary accession number(s): D6W1K6, E9P8U5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families