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Protein

GABA-specific permease

Gene

UGA4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for high-affinity, high-specificity GABA transport. Also transports putrescine.1 Publication

GO - Molecular functioni

  • antiporter activity Source: GO_Central
  • gamma-aminobutyric acid:proton symporter activity Source: SGD
  • L-amino acid transmembrane transporter activity Source: GO_Central
  • putrescine transmembrane transporter activity Source: SGD

GO - Biological processi

  • gamma-aminobutyric acid transport Source: SGD
  • putrescine transport Source: SGD
  • transmembrane transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29592-MONOMER.

Protein family/group databases

TCDBi2.A.3.4.3. the amino acid-polyamine-organocation (apc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
GABA-specific permease
Alternative name(s):
GABA-specific transport protein
Gene namesi
Name:UGA4
Ordered Locus Names:YDL210W
ORF Names:D1037
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL210W.
SGDiS000002369. UGA4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 73CytoplasmicSequence analysisAdd BLAST73
Transmembranei74 – 94HelicalSequence analysisAdd BLAST21
Topological domaini95 – 105VacuolarSequence analysisAdd BLAST11
Transmembranei106 – 126HelicalSequence analysisAdd BLAST21
Topological domaini127 – 153CytoplasmicSequence analysisAdd BLAST27
Transmembranei154 – 174HelicalSequence analysisAdd BLAST21
Topological domaini175 – 198VacuolarSequence analysisAdd BLAST24
Transmembranei199 – 219HelicalSequence analysisAdd BLAST21
Topological domaini220 – 228CytoplasmicSequence analysis9
Transmembranei229 – 249HelicalSequence analysisAdd BLAST21
Topological domaini250 – 271VacuolarSequence analysisAdd BLAST22
Transmembranei272 – 292HelicalSequence analysisAdd BLAST21
Topological domaini293 – 312CytoplasmicSequence analysisAdd BLAST20
Transmembranei313 – 333HelicalSequence analysisAdd BLAST21
Topological domaini334 – 364VacuolarSequence analysisAdd BLAST31
Transmembranei365 – 385HelicalSequence analysisAdd BLAST21
Topological domaini386 – 416CytoplasmicSequence analysisAdd BLAST31
Transmembranei417 – 437HelicalSequence analysisAdd BLAST21
Topological domaini438 – 441VacuolarSequence analysis4
Transmembranei442 – 462HelicalSequence analysisAdd BLAST21
Topological domaini463 – 482CytoplasmicSequence analysisAdd BLAST20
Transmembranei483 – 503HelicalSequence analysisAdd BLAST21
Topological domaini504 – 514VacuolarSequence analysisAdd BLAST11
Transmembranei515 – 535HelicalSequence analysisAdd BLAST21
Topological domaini536 – 571CytoplasmicSequence analysisAdd BLAST36

GO - Cellular componenti

  • fungal-type vacuole membrane Source: SGD
  • integral component of plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000541611 – 571GABA-specific permeaseAdd BLAST571

Expressioni

Inductioni

In the presence of GABA.

Interactioni

Protein-protein interaction databases

BioGridi31836. 36 interactors.
DIPiDIP-1857N.
IntActiP32837. 7 interactors.
MINTiMINT-409806.

Structurei

3D structure databases

ProteinModelPortaliP32837.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000067168.
HOGENOMiHOG000171300.
InParanoidiP32837.
OMAiTENHINQ.
OrthoDBiEOG092C1ZUR.

Family and domain databases

InterProiIPR002293. AA/rel_permease1.
IPR004840. Amoino_acid_permease_CS.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
PROSITEiPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32837-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMSSKNENK ISVEQRISTD IGQAYQLQGL GSNLRSIRSK TGAGEVNYID
60 70 80 90 100
AAKSVNDNQL LAEIGYKQEL KRQFSTLQVF GIAFSIMGLL PSIASVMGGG
110 120 130 140 150
LGGGPATLVW GWFVAAFFIL LVGITMAEHA SSIPTAGGLY YWTYYYAPEG
160 170 180 190 200
YKEIISFIIG CSNSLALAAG VCSIDYGLAE EIAAAVTLTK DGNFEVTSGK
210 220 230 240 250
LYGIFAGAVV VMCICTCVAS GAIARLQTLS IFANLFIIVL LFIALPIGTK
260 270 280 290 300
HRMGGFNDGD FIFGKYENLS DWNNGWQFCL AGFMPAVWTI GSFDSCVHQS
310 320 330 340 350
EEAKDAKKSV PIGIISSIAV CWILGWLIII CLMACINPDI DSVLDSKYGF
360 370 380 390 400
ALAQIIYDSL GKKWAIAFMS LIAFCQFLMG ASITTAVSRQ VWAFSRDNGL
410 420 430 440 450
PLSKYIKRVD SKYSVPFFAI LAACVGSLIL GLLCLIDDAA TDALFSLAVA
460 470 480 490 500
GNNLAWSTPT VFRLTSGRDL FRPGPFYLGK IWSPIVAWTG VAFQLFIIIL
510 520 530 540 550
VMFPSQQHGI TKSTMNYACV IGPGIWILAG IYYKVYKKKY YHGPATNLSD
560 570
DDYTEAVGAD VIDTIMSKQE P
Length:571
Mass (Da):61,873
Last modified:October 1, 1993 - v1
Checksum:i051B5828654EC784
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66472 Genomic DNA. Translation: CAA47101.1.
X99000 Genomic DNA. Translation: CAA67481.1.
Z74258 Genomic DNA. Translation: CAA98788.1.
BK006938 Genomic DNA. Translation: DAA11654.1.
PIRiS30253.
RefSeqiNP_010071.1. NM_001180270.1.

Genome annotation databases

EnsemblFungiiYDL210W; YDL210W; YDL210W.
GeneIDi851317.
KEGGisce:YDL210W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66472 Genomic DNA. Translation: CAA47101.1.
X99000 Genomic DNA. Translation: CAA67481.1.
Z74258 Genomic DNA. Translation: CAA98788.1.
BK006938 Genomic DNA. Translation: DAA11654.1.
PIRiS30253.
RefSeqiNP_010071.1. NM_001180270.1.

3D structure databases

ProteinModelPortaliP32837.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31836. 36 interactors.
DIPiDIP-1857N.
IntActiP32837. 7 interactors.
MINTiMINT-409806.

Protein family/group databases

TCDBi2.A.3.4.3. the amino acid-polyamine-organocation (apc) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL210W; YDL210W; YDL210W.
GeneIDi851317.
KEGGisce:YDL210W.

Organism-specific databases

EuPathDBiFungiDB:YDL210W.
SGDiS000002369. UGA4.

Phylogenomic databases

GeneTreeiENSGT00530000067168.
HOGENOMiHOG000171300.
InParanoidiP32837.
OMAiTENHINQ.
OrthoDBiEOG092C1ZUR.

Enzyme and pathway databases

BioCyciYEAST:G3O-29592-MONOMER.

Miscellaneous databases

PROiP32837.

Family and domain databases

InterProiIPR002293. AA/rel_permease1.
IPR004840. Amoino_acid_permease_CS.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
PROSITEiPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUGA4_YEAST
AccessioniPrimary (citable) accession number: P32837
Secondary accession number(s): D6VRE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 2, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

It requires NPR (nitrogen permease reactivator protein) for its full activity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.