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Protein

GTP-binding nuclear protein GSP2/CNR2

Gene

GSP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Not essential for cell viability.

Miscellaneous

Present with 2460 molecules/cell in log phase SD medium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei71GTP; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi21 – 28GTPBy similarity8
Nucleotide bindingi125 – 128GTPBy similarity4
Nucleotide bindingi153 – 155GTPBy similarity3

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • nucleus organization Source: SGD
  • protein import into nucleus Source: GO_Central
  • ribosomal subunit export from nucleus Source: GO_Central

Keywordsi

Biological processProtein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33695-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding nuclear protein GSP2/CNR2
Gene namesi
Name:GSP2
Synonyms:CNR2
Ordered Locus Names:YOR185C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR185C.
SGDiS000005711. GSP2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002087342 – 220GTP-binding nuclear protein GSP2/CNR2Add BLAST219

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP32836.
PaxDbiP32836.
PRIDEiP32836.

PTM databases

iPTMnetiP32836.

Expressioni

Inductioni

GSP2 expression exhibits carbon source dependency.

Interactioni

Subunit structurei

Found in a nuclear export complex with RanGTP, exportin and pre-miRNA (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
MOG1P471233EBI-7934,EBI-11145

Protein-protein interaction databases

BioGridi34580. 109 interactors.
DIPiDIP-1357N.
IntActiP32836. 21 interactors.
MINTiP32836.
STRINGi4932.YOR185C.

Chemistry databases

BindingDBiP32836.

Structurei

3D structure databases

ProteinModelPortaliP32836.
SMRiP32836.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ran family.Curated

Phylogenomic databases

GeneTreeiENSGT00840000129836.
HOGENOMiHOG000216664.
InParanoidiP32836.
KOiK07936.
OMAiAGDANIH.
OrthoDBiEOG092C4F6L.

Family and domain databases

InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR002041. Ran_GTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
PANTHERiPTHR24071. PTHR24071. 1 hit.
PfamiView protein in Pfam
PF00071. Ras. 1 hit.
PRINTSiPR00627. GTPRANTC4.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiView protein in PROSITE
PS51418. RAN. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32836-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAPAQNNAE VPTFKLVLVG DGGTGKTTFV KRHLTGEFEK KYIATIGVEV
60 70 80 90 100
HPLSFYTNFG EIKFDVWDTA GQEKFGGLRD GYYINAQCAI IMFDVTSRIT
110 120 130 140 150
YKNVPNWHRD LVRVCENIPI VLCGNKVDVK ERKVKAKTIT FHRKKNLQYY
160 170 180 190 200
DISAKSNYNF EKPFLWLARK LAGNPQLEFV ASPALAPPEV QVDEQLMHQY
210 220
QQEMDQATAL PLPDEDDADL
Length:220
Mass (Da):24,991
Last modified:October 1, 1993 - v1
Checksum:i402EEE0F140B37A1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08691 Genomic DNA. Translation: AAA34654.1.
X71946 Genomic DNA. Translation: CAA50748.1.
Z75093 Genomic DNA. Translation: CAA99394.1.
AY693117 Genomic DNA. Translation: AAT93136.1.
BK006948 Genomic DNA. Translation: DAA10957.1.
PIRiS35505.
RefSeqiNP_014828.1. NM_001183604.1.

Genome annotation databases

EnsemblFungiiYOR185C; YOR185C; YOR185C.
GeneIDi854357.
KEGGisce:YOR185C.

Similar proteinsi

Entry informationi

Entry nameiGSP2_YEAST
AccessioniPrimary (citable) accession number: P32836
Secondary accession number(s): D6W2P1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: March 28, 2018
This is version 162 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome