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Protein

GTP-binding nuclear protein GSP1/CNR1

Gene

GSP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Essential for cell viability. By analogy with Ras, Ran may be activated when GTP is exchanged for bound GDP by RCC1 and inactivated when GTP is hydrolyzed by Ran upon activation by RanGAP1.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei70GTP; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi20 – 27GTPBy similarity8
Nucleotide bindingi124 – 127GTPBy similarity4
Nucleotide bindingi152 – 154GTPBy similarity3

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • nucleocytoplasmic transport Source: SGD
  • nucleus organization Source: SGD
  • protein import into nucleus Source: GO_Central
  • regulation of chromatin silencing at telomere Source: SGD
  • ribosomal subunit export from nucleus Source: GO_Central

Keywordsi

Biological processProtein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32388-MONOMER

Protein family/group databases

TCDBi9.A.50.1.1 the nuclear t-rna exporter (t-exporter) family

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding nuclear protein GSP1/CNR1
Alternative name(s):
Chromosome stability protein 17
GTPase Ran homolog
Genetic suppressor of PRP20-1
Gene namesi
Name:GSP1
Synonyms:CNR1, CST17
Ordered Locus Names:YLR293C
ORF Names:L8003.19
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR293C
SGDiS000004284 GSP1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002087332 – 219GTP-binding nuclear protein GSP1/CNR1Add BLAST218

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei2PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP32835
PaxDbiP32835
PRIDEiP32835

2D gel databases

SWISS-2DPAGEP32835

PTM databases

iPTMnetiP32835

Interactioni

Subunit structurei

Found in a nuclear export complex with RanGTP, exportin and pre-miRNA (By similarity). Forms a complex with YRB1. Interacts with BUD5, CEX1, RRP12, SRM1, and DIS3/RRP44.By similarity5 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi31559, 324 interactors
DIPiDIP-747N
IntActiP32835, 32 interactors
MINTiP32835
STRINGi4932.YLR293C

Chemistry databases

BindingDBiP32835

Structurei

Secondary structure

1219
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi12 – 19Combined sources8
Helixi25 – 34Combined sources10
Beta strandi46 – 56Combined sources11
Beta strandi59 – 68Combined sources10
Helixi72 – 74Combined sources3
Helixi78 – 82Combined sources5
Beta strandi87 – 93Combined sources7
Helixi97 – 101Combined sources5
Helixi103 – 113Combined sources11
Beta strandi114 – 116Combined sources3
Beta strandi119 – 124Combined sources6
Beta strandi128 – 130Combined sources3
Helixi135 – 137Combined sources3
Helixi141 – 144Combined sources4
Beta strandi147 – 150Combined sources4
Turni153 – 155Combined sources3
Turni157 – 160Combined sources4
Helixi161 – 171Combined sources11
Helixi200 – 207Combined sources8
Turni214 – 216Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2X19X-ray2.80A8-179[»]
3ICQX-ray3.20B/C9-179[»]
3M1IX-ray2.00A1-219[»]
3WYGX-ray2.15A1-182[»]
ProteinModelPortaliP32835
SMRiP32835
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32835

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ran family.Curated

Phylogenomic databases

GeneTreeiENSGT00840000129836
HOGENOMiHOG000216664
InParanoidiP32835
KOiK07936
OMAiYNVWDTA
OrthoDBiEOG092C4F6L

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR002041 Ran_GTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PANTHERiPTHR24071 PTHR24071, 1 hit
PfamiView protein in Pfam
PF00071 Ras, 1 hit
PRINTSiPR00627 GTPRANTC4
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51418 RAN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32835-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAPAANGEV PTFKLVLVGD GGTGKTTFVK RHLTGEFEKK YIATIGVEVH
60 70 80 90 100
PLSFYTNFGE IKFDVWDTAG QEKFGGLRDG YYINAQCAII MFDVTSRITY
110 120 130 140 150
KNVPNWHRDL VRVCENIPIV LCGNKVDVKE RKVKAKTITF HRKKNLQYYD
160 170 180 190 200
ISAKSNYNFE KPFLWLARKL AGNPQLEFVA SPALAPPEVQ VDEQLMQQYQ
210
QEMEQATALP LPDEDDADL
Length:219
Mass (Da):24,810
Last modified:October 1, 1993 - v1
Checksum:iC9D027033F8C70E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71945 Genomic DNA Translation: CAA50747.1
L08690 Genomic DNA Translation: AAA34653.1
U17243 Genomic DNA Translation: AAB67339.1
AY558363 Genomic DNA Translation: AAS56689.1
BK006945 Genomic DNA Translation: DAA09605.1
PIRiS35504
RefSeqiNP_013396.1, NM_001182181.1

Genome annotation databases

EnsemblFungiiYLR293C; YLR293C; YLR293C
GeneIDi851000
KEGGisce:YLR293C

Similar proteinsi

Entry informationi

Entry nameiGSP1_YEAST
AccessioniPrimary (citable) accession number: P32835
Secondary accession number(s): D6VYT9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 23, 2018
This is version 179 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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