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Protein

GTP-binding nuclear protein GSP1/CNR1

Gene

GSP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Essential for cell viability. By analogy with Ras, Ran may be activated when GTP is exchanged for bound GDP by RCC1 and inactivated when GTP is hydrolyzed by Ran upon activation by RanGAP1.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi19 – 26GTPBy similarity8
Nucleotide bindingi67 – 71GTPBy similarity5
Nucleotide bindingi124 – 127GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • nucleocytoplasmic transport Source: SGD
  • nucleus organization Source: SGD
  • protein import into nucleus Source: GO_Central
  • regulation of chromatin silencing at telomere Source: SGD
  • ribosomal subunit export from nucleus Source: GO_Central
  • small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32388-MONOMER.
ReactomeiR-SCE-203927. MicroRNA (miRNA) biogenesis.

Protein family/group databases

TCDBi9.A.50.1.1. the nuclear t-rna exporter (t-exporter) family.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding nuclear protein GSP1/CNR1
Alternative name(s):
Chromosome stability protein 17
GTPase Ran homolog
Genetic suppressor of PRP20-1
Gene namesi
Name:GSP1
Synonyms:CNR1, CST17
Ordered Locus Names:YLR293C
ORF Names:L8003.19
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR293C.
SGDiS000004284. GSP1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002087332 – 219GTP-binding nuclear protein GSP1/CNR1Add BLAST218

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei2PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP32835.
PRIDEiP32835.

2D gel databases

SWISS-2DPAGEP32835.

PTM databases

iPTMnetiP32835.

Interactioni

Subunit structurei

Forms a complex with YRB1. Interacts with BUD5, CEX1, RRP12, SRM1, and DIS3/RRP44.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BLM10P435832EBI-7926,EBI-22761
BUD5P253002EBI-7926,EBI-3853
KAP114P530673EBI-7926,EBI-9174

Protein-protein interaction databases

BioGridi31559. 97 interactors.
DIPiDIP-747N.
IntActiP32835. 19 interactors.
MINTiMINT-384649.

Chemistry databases

BindingDBiP32835.

Structurei

Secondary structure

1219
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi12 – 19Combined sources8
Helixi25 – 34Combined sources10
Beta strandi46 – 56Combined sources11
Beta strandi59 – 68Combined sources10
Helixi72 – 74Combined sources3
Helixi78 – 82Combined sources5
Beta strandi87 – 93Combined sources7
Helixi97 – 101Combined sources5
Helixi103 – 113Combined sources11
Beta strandi114 – 116Combined sources3
Beta strandi119 – 124Combined sources6
Beta strandi128 – 130Combined sources3
Helixi135 – 137Combined sources3
Helixi141 – 144Combined sources4
Beta strandi147 – 150Combined sources4
Turni153 – 155Combined sources3
Turni157 – 160Combined sources4
Helixi161 – 171Combined sources11
Helixi200 – 207Combined sources8
Turni214 – 216Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2X19X-ray2.80A8-179[»]
3ICQX-ray3.20B/C9-179[»]
3M1IX-ray2.00A1-219[»]
3WYGX-ray2.15A1-182[»]
ProteinModelPortaliP32835.
SMRiP32835.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32835.

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ran family.Curated

Phylogenomic databases

GeneTreeiENSGT00840000129836.
HOGENOMiHOG000216664.
InParanoidiP32835.
KOiK07936.
OMAiNIQYFEI.
OrthoDBiEOG092C4F6L.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR002041. Ran_GTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00627. GTPRANTC4.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51418. RAN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32835-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAPAANGEV PTFKLVLVGD GGTGKTTFVK RHLTGEFEKK YIATIGVEVH
60 70 80 90 100
PLSFYTNFGE IKFDVWDTAG QEKFGGLRDG YYINAQCAII MFDVTSRITY
110 120 130 140 150
KNVPNWHRDL VRVCENIPIV LCGNKVDVKE RKVKAKTITF HRKKNLQYYD
160 170 180 190 200
ISAKSNYNFE KPFLWLARKL AGNPQLEFVA SPALAPPEVQ VDEQLMQQYQ
210
QEMEQATALP LPDEDDADL
Length:219
Mass (Da):24,810
Last modified:October 1, 1993 - v1
Checksum:iC9D027033F8C70E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71945 Genomic DNA. Translation: CAA50747.1.
L08690 Genomic DNA. Translation: AAA34653.1.
U17243 Genomic DNA. Translation: AAB67339.1.
AY558363 Genomic DNA. Translation: AAS56689.1.
BK006945 Genomic DNA. Translation: DAA09605.1.
PIRiS35504.
RefSeqiNP_013396.1. NM_001182181.1.

Genome annotation databases

EnsemblFungiiYLR293C; YLR293C; YLR293C.
GeneIDi851000.
KEGGisce:YLR293C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71945 Genomic DNA. Translation: CAA50747.1.
L08690 Genomic DNA. Translation: AAA34653.1.
U17243 Genomic DNA. Translation: AAB67339.1.
AY558363 Genomic DNA. Translation: AAS56689.1.
BK006945 Genomic DNA. Translation: DAA09605.1.
PIRiS35504.
RefSeqiNP_013396.1. NM_001182181.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2X19X-ray2.80A8-179[»]
3ICQX-ray3.20B/C9-179[»]
3M1IX-ray2.00A1-219[»]
3WYGX-ray2.15A1-182[»]
ProteinModelPortaliP32835.
SMRiP32835.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31559. 97 interactors.
DIPiDIP-747N.
IntActiP32835. 19 interactors.
MINTiMINT-384649.

Chemistry databases

BindingDBiP32835.

Protein family/group databases

TCDBi9.A.50.1.1. the nuclear t-rna exporter (t-exporter) family.

PTM databases

iPTMnetiP32835.

2D gel databases

SWISS-2DPAGEP32835.

Proteomic databases

MaxQBiP32835.
PRIDEiP32835.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR293C; YLR293C; YLR293C.
GeneIDi851000.
KEGGisce:YLR293C.

Organism-specific databases

EuPathDBiFungiDB:YLR293C.
SGDiS000004284. GSP1.

Phylogenomic databases

GeneTreeiENSGT00840000129836.
HOGENOMiHOG000216664.
InParanoidiP32835.
KOiK07936.
OMAiNIQYFEI.
OrthoDBiEOG092C4F6L.

Enzyme and pathway databases

BioCyciYEAST:G3O-32388-MONOMER.
ReactomeiR-SCE-203927. MicroRNA (miRNA) biogenesis.

Miscellaneous databases

EvolutionaryTraceiP32835.
PROiP32835.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR002041. Ran_GTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00627. GTPRANTC4.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51418. RAN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSP1_YEAST
AccessioniPrimary (citable) accession number: P32835
Secondary accession number(s): D6VYT9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 30, 2016
This is version 166 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.