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P32832 (RSC7_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chromatin structure-remodeling complex subunit RSC7
Alternative name(s):
Nuclear protein localization protein 6
Remodel the structure of chromatin complex subunit 7
Gene names
Name:NPL6
Synonyms:RSC7
Ordered Locus Names:YMR091C
ORF Names:YM9582.16C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length435 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. Together with HTL1, LDB7, RSC3, RSC30 components, defines a fungal-specific module within the RSC complex that plays a role in many cellular functions including the maintenance of cell wall integrity. Acidic protein important for nuclear protein localization. Ref.6

Subunit structure

Interacts with ARP7, ARP9, RSC3, RSC8, RSC30 and STH1. Component of the two forms of the RSC complex composed of at least either RSC1 or RSC2, and ARP7, ARP9, LDB7, NPL6, RSC3, RSC30, RSC4, RSC58, RSC6, RSC8, RSC9, SFH1, STH1, HTL1 and probably RTT102. The complexes interact with histone and histone variant components of centromeric chromatin. Component of a fungal-specific module (HTL1-LDB7-NPL6-RSC3-RSC30) within the RSC complex. Ref.6

Subcellular location

Nucleus Ref.4.

Miscellaneous

Present with 3510 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the RSC7/SWP82 family. RSC7 subfamily.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

HSP82P028292EBI-12202,EBI-8659
RSC8P436097EBI-12202,EBI-23005

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 435435Chromatin structure-remodeling complex subunit RSC7
PRO_0000057945

Regions

Region248 – 435188Functional region; able to complement all NPL6 null allele phenotypes

Amino acid modifications

Modified residue861Phosphoserine Ref.8

Sequences

Sequence LengthMass (Da)Tools
P32832 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 57B416BFB340422B

FASTA43549,651
        10         20         30         40         50         60 
MSDSEGGLAS EVEHEKRSRS TSNRPNYAID TEDLDIDEND ENEDDDYREE EANEGVNEEE 

        70         80         90        100        110        120 
ISDEEEQINK SGRNKRRHVD EEEDLSEDKG VTRSRNRSKF KKPVFPGIDD AEENLNPLKV 

       130        140        150        160        170        180 
VNEEYVLPDD PEGETKITAD GDLLGGREFL VRTFTLTEKG NRKFMLATEP ARIVGFRDSY 

       190        200        210        220        230        240 
LFFQTHPNLY KFILNQTQKN DLIDRGVLPY SYRNRQIALV TARGVFKEFG AKIIRGGKHI 

       250        260        270        280        290        300 
TDDYYASELR TKGNVIEGKL AGDPIDKSAR ALETMMYPAS ENGINPAKNQ VEFFEHRPHG 

       310        320        330        340        350        360 
HMSNSNIIAS GSKLSSTNWL YQHSAACSRF NSDLFYDRVK VLLVDQQGLR DAYTNILHIP 

       370        380        390        400        410        420 
ESTQSTTVLG WRRSKNDSPS DTSIVYETVI HDNDLNKPKT GLSEIPKEIY EDVVDEDVLR 

       430 
AITEQQNFEK CNEYI 

« Hide

References

« Hide 'large scale' references
[1]"An acidic protein important for nuclear protein localization."
Chiang A.N., Silver P.A.
Submitted (AUG-1992) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"The RSC chromatin remodeling complex bears an essential fungal-specific protein module with broad functional roles."
Wilson B., Erdjument-Bromage H., Tempst P., Cairns B.R.
Genetics 172:795-809(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN THE RSC COMPLEX, INTERACTION WITH ARP7; ARP9; RSC3; RSC8; RSC30 AND STH1.
[7]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-86, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M98434 Genomic DNA. Translation: AAA34817.1.
Z49259 Genomic DNA. Translation: CAA89238.1.
BK006946 Genomic DNA. Translation: DAA09988.1.
PIRS30792.
RefSeqNP_013809.1. NM_001182591.1.

3D structure databases

ProteinModelPortalP32832.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35266. 121 interactions.
DIPDIP-1477N.
IntActP32832. 61 interactions.
MINTMINT-401622.
STRING4932.YMR091C.

Proteomic databases

PaxDbP32832.
PeptideAtlasP32832.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR091C; YMR091C; YMR091C.
GeneID855116.
KEGGsce:YMR091C.

Organism-specific databases

CYGDYMR091c.
SGDS000004697. NPL6.

Phylogenomic databases

eggNOGNOG79203.
GeneTreeENSGT00530000067789.
HOGENOMHOG000141761.
KOK11761.
OMAVIDDFWE.
OrthoDBEOG7C8GSS.

Enzyme and pathway databases

BioCycYEAST:G3O-32791-MONOMER.

Gene expression databases

GenevestigatorP32832.

Family and domain databases

InterProIPR013933. CRC_Rsc7/Swp82.
[Graphical view]
PfamPF08624. CRC_subunit. 1 hit.
[Graphical view]
ProDomPD041931. CRC_Rsc7/Swp82. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Other

NextBio978461.
PROP32832.

Entry information

Entry nameRSC7_YEAST
AccessionPrimary (citable) accession number: P32832
Secondary accession number(s): D6VZR4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: April 16, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families