P32832 (RSC7_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chromatin structure-remodeling complex subunit RSC7

Alternative name(s):
Nuclear protein localization protein 6
Remodel the structure of chromatin complex subunit 7

Gene names

Name:	NPL6
Synonyms:	RSC7
Ordered Locus Names:	YMR091C
ORF Names:	YM9582.16C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. Together with HTL1, LDB7, RSC3, RSC30 components, defines a fungal-specific module within the RSC complex that plays a role in many cellular functions including the maintenance of cell wall integrity. Acidic protein important for nuclear protein localization. Ref.6
Subunit structure	Interacts with ARP7, ARP9, RSC3, RSC8, RSC30 and STH1. Component of the two forms of the RSC complex composed of at least either RSC1 or RSC2, and ARP7, ARP9, LDB7, NPL6, RSC3, RSC30, RSC4, RSC58, RSC6, RSC8, RSC9, SFH1, STH1, HTL1 and probably RTT102. The complexes interact with histone and histone variant components of centromeric chromatin. Component of a fungal-specific module (HTL1-LDB7-NPL6-RSC3-RSC30) within the RSC complex. Ref.6
Subcellular location	Nucleus Ref.4.
Miscellaneous	Present with 3510 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the RSC7/SWP82 family. RSC7 subfamily.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Chromatin regulator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ATP-dependent chromatin remodeling Inferred from direct assay PubMed 12183366. Source: SGD nucleosome disassembly Inferred from direct assay PubMed 16492771. Source: SGD protein import into nucleus Inferred from mutant phenotype PubMed 2556404. Source: SGD regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW response to DNA damage stimulus Inferred from mutant phenotype PubMed 12482937. Source: SGD transcription elongation from RNA polymerase II promoter Inferred from direct assay PubMed 17081996. Source: SGD
Cellular_component	RSC complex Inferred from direct assay PubMed 10619019PubMed 12183366PubMed 14729968. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
HSP82	P02829	2	EBI-12202,EBI-8659
RSC8	P43609	7	EBI-12202,EBI-23005

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 435	435	Chromatin structure-remodeling complex subunit RSC7		PRO_0000057945
Regions
	Region	248 – 435	188	Functional region; able to complement all NPL6 null allele phenotypes
Amino acid modifications
	Modified residue	86	1	Phosphoserine Ref.7 Ref.8 Ref.9
	Modified residue	155	1	Phosphothreonine Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P32832 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 57B416BFB340422B	FASTA	435	49,651
	10 20 30 40 50 60 MSDSEGGLAS EVEHEKRSRS TSNRPNYAID TEDLDIDEND ENEDDDYREE EANEGVNEEE 70 80 90 100 110 120 ISDEEEQINK SGRNKRRHVD EEEDLSEDKG VTRSRNRSKF KKPVFPGIDD AEENLNPLKV 130 140 150 160 170 180 VNEEYVLPDD PEGETKITAD GDLLGGREFL VRTFTLTEKG NRKFMLATEP ARIVGFRDSY 190 200 210 220 230 240 LFFQTHPNLY KFILNQTQKN DLIDRGVLPY SYRNRQIALV TARGVFKEFG AKIIRGGKHI 250 260 270 280 290 300 TDDYYASELR TKGNVIEGKL AGDPIDKSAR ALETMMYPAS ENGINPAKNQ VEFFEHRPHG 310 320 330 340 350 360 HMSNSNIIAS GSKLSSTNWL YQHSAACSRF NSDLFYDRVK VLLVDQQGLR DAYTNILHIP 370 380 390 400 410 420 ESTQSTTVLG WRRSKNDSPS DTSIVYETVI HDNDLNKPKT GLSEIPKEIY EDVVDEDVLR 430 AITEQQNFEK CNEYI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An acidic protein important for nuclear protein localization." Chiang A.N., Silver P.A. Submitted (AUG-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"The RSC chromatin remodeling complex bears an essential fungal-specific protein module with broad functional roles." Wilson B., Erdjument-Bromage H., Tempst P., Cairns B.R. Genetics 172:795-809(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE RSC COMPLEX, INTERACTION WITH ARP7; ARP9; RSC3; RSC8; RSC30 AND STH1.
[7]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-86, MASS SPECTROMETRY.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-86, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-86 AND THR-155, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M98434 Genomic DNA. Translation: AAA34817.1. Z49259 Genomic DNA. Translation: CAA89238.1. BK006946 Genomic DNA. Translation: DAA09988.1.
PIR	S30792.
RefSeq	NP_013809.1. NM_001182591.1.
3D structure databases
ProteinModelPortal	P32832.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1477N.
IntAct	P32832. 61 interactions.
MINT	MINT-401622.
STRING	4932.YMR091C.
Proteomic databases
PaxDb	P32832.
PeptideAtlas	P32832.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR091C; YMR091C; YMR091C.
GeneID	855116.
KEGG	sce:YMR091C.
Organism-specific databases
CYGD	YMR091c.
SGD	S000004697. NPL6.
Phylogenomic databases
eggNOG	NOG79203.
GeneTree	ENSGT00530000067789.
HOGENOM	HOG000141761.
KO	K11761.
OMA	YLLFKTH.
OrthoDB	EOG4HB1W2.
Gene expression databases
Genevestigator	P32832.
GermOnline	YMR091C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013933. CRC_Rsc7/Swp82. [Graphical view]
Pfam	PF08624. CRC_subunit. 1 hit. [Graphical view]
ProDom	PD041931. CRC_Rsc7/Swp82. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...
Other
NextBio	978461.

Entry information

Entry name

RSC7_YEAST

Accession

Primary (citable) accession number: P32832
Secondary accession number(s): D6VZR4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	April 3, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program