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Protein

Chromatin structure-remodeling complex subunit RSC7

Gene

NPL6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. Together with HTL1, LDB7, RSC3, RSC30 components, defines a fungal-specific module within the RSC complex that plays a role in many cellular functions including the maintenance of cell wall integrity. Acidic protein important for nuclear protein localization.1 Publication

Miscellaneous

Present with 3510 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

  • ATP-dependent chromatin remodeling Source: SGD
  • nucleosome disassembly Source: SGD
  • regulation of transcription, DNA-templated Source: GO_Central
  • transcription elongation from RNA polymerase II promoter Source: SGD

Keywordsi

Molecular functionChromatin regulator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-32791-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Chromatin structure-remodeling complex subunit RSC7
Alternative name(s):
Nuclear protein localization protein 6
Remodel the structure of chromatin complex subunit 7
Gene namesi
Name:NPL6
Synonyms:RSC7
Ordered Locus Names:YMR091C
ORF Names:YM9582.16C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR091C
SGDiS000004697 NPL6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000579451 – 435Chromatin structure-remodeling complex subunit RSC7Add BLAST435

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei86PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32832
PaxDbiP32832
PRIDEiP32832

PTM databases

iPTMnetiP32832

Interactioni

Subunit structurei

Interacts with ARP7, ARP9, RSC3, RSC8, RSC30 and STH1. Component of the two forms of the RSC complex composed of at least either RSC1 or RSC2, and ARP7, ARP9, LDB7, NPL6, RSC3, RSC30, RSC4, RSC58, RSC6, RSC8, RSC9, SFH1, STH1, HTL1 and probably RTT102. The complexes interact with histone and histone variant components of centromeric chromatin. Component of a fungal-specific module (HTL1-LDB7-NPL6-RSC3-RSC30) within the RSC complex.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35266, 125 interactors
DIPiDIP-1477N
IntActiP32832, 62 interactors
MINTiP32832
STRINGi4932.YMR091C

Structurei

3D structure databases

ProteinModelPortaliP32832
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni248 – 435Functional region; able to complement all NPL6 null allele phenotypesAdd BLAST188

Sequence similaritiesi

Belongs to the RSC7/SWP82 family. RSC7 subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00530000067789
HOGENOMiHOG000141761
InParanoidiP32832
KOiK11761
OMAiVIDDFWE
OrthoDBiEOG092C31JT

Family and domain databases

InterProiView protein in InterPro
IPR013933 CRC_Rsc7/Swp82
PfamiView protein in Pfam
PF08624 CRC_subunit, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD041931 CRC_Rsc7/Swp82, 1 hit

Sequencei

Sequence statusi: Complete.

P32832-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDSEGGLAS EVEHEKRSRS TSNRPNYAID TEDLDIDEND ENEDDDYREE
60 70 80 90 100
EANEGVNEEE ISDEEEQINK SGRNKRRHVD EEEDLSEDKG VTRSRNRSKF
110 120 130 140 150
KKPVFPGIDD AEENLNPLKV VNEEYVLPDD PEGETKITAD GDLLGGREFL
160 170 180 190 200
VRTFTLTEKG NRKFMLATEP ARIVGFRDSY LFFQTHPNLY KFILNQTQKN
210 220 230 240 250
DLIDRGVLPY SYRNRQIALV TARGVFKEFG AKIIRGGKHI TDDYYASELR
260 270 280 290 300
TKGNVIEGKL AGDPIDKSAR ALETMMYPAS ENGINPAKNQ VEFFEHRPHG
310 320 330 340 350
HMSNSNIIAS GSKLSSTNWL YQHSAACSRF NSDLFYDRVK VLLVDQQGLR
360 370 380 390 400
DAYTNILHIP ESTQSTTVLG WRRSKNDSPS DTSIVYETVI HDNDLNKPKT
410 420 430
GLSEIPKEIY EDVVDEDVLR AITEQQNFEK CNEYI
Length:435
Mass (Da):49,651
Last modified:October 1, 1993 - v1
Checksum:i57B416BFB340422B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98434 Genomic DNA Translation: AAA34817.1
Z49259 Genomic DNA Translation: CAA89238.1
BK006946 Genomic DNA Translation: DAA09988.1
PIRiS30792
RefSeqiNP_013809.1, NM_001182591.1

Genome annotation databases

EnsemblFungiiYMR091C; YMR091C; YMR091C
GeneIDi855116
KEGGisce:YMR091C

Similar proteinsi

Entry informationi

Entry nameiRSC7_YEAST
AccessioniPrimary (citable) accession number: P32832
Secondary accession number(s): D6VZR4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 23, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health