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Protein

Chromatin structure-remodeling complex subunit RSC7

Gene

NPL6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. Together with HTL1, LDB7, RSC3, RSC30 components, defines a fungal-specific module within the RSC complex that plays a role in many cellular functions including the maintenance of cell wall integrity. Acidic protein important for nuclear protein localization.1 Publication

GO - Biological processi

  1. ATP-dependent chromatin remodeling Source: SGD
  2. chromatin remodeling Source: SGD
  3. nucleosome disassembly Source: SGD
  4. protein import into nucleus Source: SGD
  5. regulation of transcription, DNA-templated Source: UniProtKB-KW
  6. transcription elongation from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-32791-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chromatin structure-remodeling complex subunit RSC7
Alternative name(s):
Nuclear protein localization protein 6
Remodel the structure of chromatin complex subunit 7
Gene namesi
Name:NPL6
Synonyms:RSC7
Ordered Locus Names:YMR091C
ORF Names:YM9582.16C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XIII

Organism-specific databases

CYGDiYMR091c.
SGDiS000004697. NPL6.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. RSC complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 435435Chromatin structure-remodeling complex subunit RSC7PRO_0000057945Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei86 – 861Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32832.
PaxDbiP32832.
PeptideAtlasiP32832.

Expressioni

Gene expression databases

GenevestigatoriP32832.

Interactioni

Subunit structurei

Interacts with ARP7, ARP9, RSC3, RSC8, RSC30 and STH1. Component of the two forms of the RSC complex composed of at least either RSC1 or RSC2, and ARP7, ARP9, LDB7, NPL6, RSC3, RSC30, RSC4, RSC58, RSC6, RSC8, RSC9, SFH1, STH1, HTL1 and probably RTT102. The complexes interact with histone and histone variant components of centromeric chromatin. Component of a fungal-specific module (HTL1-LDB7-NPL6-RSC3-RSC30) within the RSC complex.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
HSP82P028292EBI-12202,EBI-8659
RSC8P436097EBI-12202,EBI-23005

Protein-protein interaction databases

BioGridi35266. 124 interactions.
DIPiDIP-1477N.
IntActiP32832. 62 interactions.
MINTiMINT-401622.
STRINGi4932.YMR091C.

Structurei

3D structure databases

ProteinModelPortaliP32832.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni248 – 435188Functional region; able to complement all NPL6 null allele phenotypesAdd
BLAST

Sequence similaritiesi

Belongs to the RSC7/SWP82 family. RSC7 subfamily.Curated

Phylogenomic databases

eggNOGiNOG79203.
GeneTreeiENSGT00530000067789.
HOGENOMiHOG000141761.
InParanoidiP32832.
KOiK11761.
OMAiVIDDFWE.
OrthoDBiEOG7C8GSS.

Family and domain databases

InterProiIPR013933. CRC_Rsc7/Swp82.
[Graphical view]
PfamiPF08624. CRC_subunit. 1 hit.
[Graphical view]
ProDomiPD041931. CRC_Rsc7/Swp82. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

P32832-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDSEGGLAS EVEHEKRSRS TSNRPNYAID TEDLDIDEND ENEDDDYREE
60 70 80 90 100
EANEGVNEEE ISDEEEQINK SGRNKRRHVD EEEDLSEDKG VTRSRNRSKF
110 120 130 140 150
KKPVFPGIDD AEENLNPLKV VNEEYVLPDD PEGETKITAD GDLLGGREFL
160 170 180 190 200
VRTFTLTEKG NRKFMLATEP ARIVGFRDSY LFFQTHPNLY KFILNQTQKN
210 220 230 240 250
DLIDRGVLPY SYRNRQIALV TARGVFKEFG AKIIRGGKHI TDDYYASELR
260 270 280 290 300
TKGNVIEGKL AGDPIDKSAR ALETMMYPAS ENGINPAKNQ VEFFEHRPHG
310 320 330 340 350
HMSNSNIIAS GSKLSSTNWL YQHSAACSRF NSDLFYDRVK VLLVDQQGLR
360 370 380 390 400
DAYTNILHIP ESTQSTTVLG WRRSKNDSPS DTSIVYETVI HDNDLNKPKT
410 420 430
GLSEIPKEIY EDVVDEDVLR AITEQQNFEK CNEYI
Length:435
Mass (Da):49,651
Last modified:September 30, 1993 - v1
Checksum:i57B416BFB340422B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98434 Genomic DNA. Translation: AAA34817.1.
Z49259 Genomic DNA. Translation: CAA89238.1.
BK006946 Genomic DNA. Translation: DAA09988.1.
PIRiS30792.
RefSeqiNP_013809.1. NM_001182591.1.

Genome annotation databases

EnsemblFungiiYMR091C; YMR091C; YMR091C.
GeneIDi855116.
KEGGisce:YMR091C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98434 Genomic DNA. Translation: AAA34817.1.
Z49259 Genomic DNA. Translation: CAA89238.1.
BK006946 Genomic DNA. Translation: DAA09988.1.
PIRiS30792.
RefSeqiNP_013809.1. NM_001182591.1.

3D structure databases

ProteinModelPortaliP32832.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35266. 124 interactions.
DIPiDIP-1477N.
IntActiP32832. 62 interactions.
MINTiMINT-401622.
STRINGi4932.YMR091C.

Proteomic databases

MaxQBiP32832.
PaxDbiP32832.
PeptideAtlasiP32832.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR091C; YMR091C; YMR091C.
GeneIDi855116.
KEGGisce:YMR091C.

Organism-specific databases

CYGDiYMR091c.
SGDiS000004697. NPL6.

Phylogenomic databases

eggNOGiNOG79203.
GeneTreeiENSGT00530000067789.
HOGENOMiHOG000141761.
InParanoidiP32832.
KOiK11761.
OMAiVIDDFWE.
OrthoDBiEOG7C8GSS.

Enzyme and pathway databases

BioCyciYEAST:G3O-32791-MONOMER.

Miscellaneous databases

NextBioi978461.
PROiP32832.

Gene expression databases

GenevestigatoriP32832.

Family and domain databases

InterProiIPR013933. CRC_Rsc7/Swp82.
[Graphical view]
PfamiPF08624. CRC_subunit. 1 hit.
[Graphical view]
ProDomiPD041931. CRC_Rsc7/Swp82. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "An acidic protein important for nuclear protein localization."
    Chiang A.N., Silver P.A.
    Submitted (JUL-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "The RSC chromatin remodeling complex bears an essential fungal-specific protein module with broad functional roles."
    Wilson B., Erdjument-Bromage H., Tempst P., Cairns B.R.
    Genetics 172:795-809(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE RSC COMPLEX, INTERACTION WITH ARP7; ARP9; RSC3; RSC8; RSC30 AND STH1.
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-86, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRSC7_YEAST
AccessioniPrimary (citable) accession number: P32832
Secondary accession number(s): D6VZR4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 30, 1993
Last sequence update: September 30, 1993
Last modified: March 3, 2015
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3510 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.