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P32832

- RSC7_YEAST

UniProt

P32832 - RSC7_YEAST

Protein

Chromatin structure-remodeling complex subunit RSC7

Gene

NPL6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. Together with HTL1, LDB7, RSC3, RSC30 components, defines a fungal-specific module within the RSC complex that plays a role in many cellular functions including the maintenance of cell wall integrity. Acidic protein important for nuclear protein localization.1 Publication

    GO - Molecular functioni

    1. protein binding Source: UniProtKB

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. ATP-dependent chromatin remodeling Source: SGD
    3. chromatin remodeling Source: SGD
    4. nucleosome disassembly Source: SGD
    5. protein import into nucleus Source: SGD
    6. regulation of transcription, DNA-templated Source: UniProtKB-KW
    7. transcription elongation from RNA polymerase II promoter Source: SGD

    Keywords - Molecular functioni

    Chromatin regulator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32791-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chromatin structure-remodeling complex subunit RSC7
    Alternative name(s):
    Nuclear protein localization protein 6
    Remodel the structure of chromatin complex subunit 7
    Gene namesi
    Name:NPL6
    Synonyms:RSC7
    Ordered Locus Names:YMR091C
    ORF Names:YM9582.16C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR091c.
    SGDiS000004697. NPL6.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. RSC complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 435435Chromatin structure-remodeling complex subunit RSC7PRO_0000057945Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei86 – 861Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP32832.
    PaxDbiP32832.
    PeptideAtlasiP32832.

    Expressioni

    Gene expression databases

    GenevestigatoriP32832.

    Interactioni

    Subunit structurei

    Interacts with ARP7, ARP9, RSC3, RSC8, RSC30 and STH1. Component of the two forms of the RSC complex composed of at least either RSC1 or RSC2, and ARP7, ARP9, LDB7, NPL6, RSC3, RSC30, RSC4, RSC58, RSC6, RSC8, RSC9, SFH1, STH1, HTL1 and probably RTT102. The complexes interact with histone and histone variant components of centromeric chromatin. Component of a fungal-specific module (HTL1-LDB7-NPL6-RSC3-RSC30) within the RSC complex.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HSP82P028292EBI-12202,EBI-8659
    RSC8P436097EBI-12202,EBI-23005

    Protein-protein interaction databases

    BioGridi35266. 121 interactions.
    DIPiDIP-1477N.
    IntActiP32832. 61 interactions.
    MINTiMINT-401622.
    STRINGi4932.YMR091C.

    Structurei

    3D structure databases

    ProteinModelPortaliP32832.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni248 – 435188Functional region; able to complement all NPL6 null allele phenotypesAdd
    BLAST

    Sequence similaritiesi

    Belongs to the RSC7/SWP82 family. RSC7 subfamily.Curated

    Phylogenomic databases

    eggNOGiNOG79203.
    GeneTreeiENSGT00530000067789.
    HOGENOMiHOG000141761.
    KOiK11761.
    OMAiVIDDFWE.
    OrthoDBiEOG7C8GSS.

    Family and domain databases

    InterProiIPR013933. CRC_Rsc7/Swp82.
    [Graphical view]
    PfamiPF08624. CRC_subunit. 1 hit.
    [Graphical view]
    ProDomiPD041931. CRC_Rsc7/Swp82. 1 hit.
    [Graphical view] [Entries sharing at least one domain]

    Sequencei

    Sequence statusi: Complete.

    P32832-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDSEGGLAS EVEHEKRSRS TSNRPNYAID TEDLDIDEND ENEDDDYREE    50
    EANEGVNEEE ISDEEEQINK SGRNKRRHVD EEEDLSEDKG VTRSRNRSKF 100
    KKPVFPGIDD AEENLNPLKV VNEEYVLPDD PEGETKITAD GDLLGGREFL 150
    VRTFTLTEKG NRKFMLATEP ARIVGFRDSY LFFQTHPNLY KFILNQTQKN 200
    DLIDRGVLPY SYRNRQIALV TARGVFKEFG AKIIRGGKHI TDDYYASELR 250
    TKGNVIEGKL AGDPIDKSAR ALETMMYPAS ENGINPAKNQ VEFFEHRPHG 300
    HMSNSNIIAS GSKLSSTNWL YQHSAACSRF NSDLFYDRVK VLLVDQQGLR 350
    DAYTNILHIP ESTQSTTVLG WRRSKNDSPS DTSIVYETVI HDNDLNKPKT 400
    GLSEIPKEIY EDVVDEDVLR AITEQQNFEK CNEYI 435
    Length:435
    Mass (Da):49,651
    Last modified:October 1, 1993 - v1
    Checksum:i57B416BFB340422B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M98434 Genomic DNA. Translation: AAA34817.1.
    Z49259 Genomic DNA. Translation: CAA89238.1.
    BK006946 Genomic DNA. Translation: DAA09988.1.
    PIRiS30792.
    RefSeqiNP_013809.1. NM_001182591.1.

    Genome annotation databases

    EnsemblFungiiYMR091C; YMR091C; YMR091C.
    GeneIDi855116.
    KEGGisce:YMR091C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M98434 Genomic DNA. Translation: AAA34817.1 .
    Z49259 Genomic DNA. Translation: CAA89238.1 .
    BK006946 Genomic DNA. Translation: DAA09988.1 .
    PIRi S30792.
    RefSeqi NP_013809.1. NM_001182591.1.

    3D structure databases

    ProteinModelPortali P32832.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35266. 121 interactions.
    DIPi DIP-1477N.
    IntActi P32832. 61 interactions.
    MINTi MINT-401622.
    STRINGi 4932.YMR091C.

    Proteomic databases

    MaxQBi P32832.
    PaxDbi P32832.
    PeptideAtlasi P32832.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR091C ; YMR091C ; YMR091C .
    GeneIDi 855116.
    KEGGi sce:YMR091C.

    Organism-specific databases

    CYGDi YMR091c.
    SGDi S000004697. NPL6.

    Phylogenomic databases

    eggNOGi NOG79203.
    GeneTreei ENSGT00530000067789.
    HOGENOMi HOG000141761.
    KOi K11761.
    OMAi VIDDFWE.
    OrthoDBi EOG7C8GSS.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32791-MONOMER.

    Miscellaneous databases

    NextBioi 978461.
    PROi P32832.

    Gene expression databases

    Genevestigatori P32832.

    Family and domain databases

    InterProi IPR013933. CRC_Rsc7/Swp82.
    [Graphical view ]
    Pfami PF08624. CRC_subunit. 1 hit.
    [Graphical view ]
    ProDomi PD041931. CRC_Rsc7/Swp82. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    ProtoNeti Search...

    Publicationsi

    1. "An acidic protein important for nuclear protein localization."
      Chiang A.N., Silver P.A.
      Submitted (AUG-1992) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "The RSC chromatin remodeling complex bears an essential fungal-specific protein module with broad functional roles."
      Wilson B., Erdjument-Bromage H., Tempst P., Cairns B.R.
      Genetics 172:795-809(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE RSC COMPLEX, INTERACTION WITH ARP7; ARP9; RSC3; RSC8; RSC30 AND STH1.
    7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-86, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRSC7_YEAST
    AccessioniPrimary (citable) accession number: P32832
    Secondary accession number(s): D6VZR4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3510 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3