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P32830

- TIM12_YEAST

UniProt

P32830 - TIM12_YEAST

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Protein

Mitochondrial import inner membrane translocase subunit TIM12

Gene

TIM12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Essential component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. The TIM22 complex forms a twin-pore translocase that uses the membrane potential as external driving force. In the TIM22 complex, it acts as a docking point for the soluble TIM9-TIM10 heterohexamer that guides the target proteins in transit through the aqueous mitochondrial intermembrane space.5 Publications

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phospholipid binding Source: SGD

GO - Biological processi

  1. protein import into mitochondrial inner membrane Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29057-MONOMER.
ReactomeiREACT_189012. Mitochondrial protein import.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM12
Alternative name(s):
Mitochondrial regulator of splicing 5
Gene namesi
Name:TIM12
Synonyms:MRS5
Ordered Locus Names:YBR091C
ORF Names:YBR0812
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR091c.
SGDiS000000295. TIM12.

Subcellular locationi

Mitochondrion inner membrane 2 Publications; Peripheral membrane protein 2 Publications

GO - Cellular componenti

  1. mitochondrial inner membrane Source: Reactome
  2. mitochondrial inner membrane protein insertion complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 109108Mitochondrial import inner membrane translocase subunit TIM12PRO_0000096581Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Disulfide bondi40 ↔ 66By similarity
Disulfide bondi44 ↔ 62By similarity

Keywords - PTMi

Acetylation, Disulfide bond

Proteomic databases

MaxQBiP32830.
PaxDbiP32830.
PeptideAtlasiP32830.

Expressioni

Gene expression databases

GenevestigatoriP32830.

Interactioni

Subunit structurei

Component of the TIM22 complex, whose core is composed of TIM18, TIM22 and TIM54, associated with the peripheral proteins MRS5/TIM12 and the 70 kDa heterohexamer composed of TIM9 and TIM10 (or TIM8 and TIM13). Interacts directly with both the TIM22 protein and the TIM9-TIM10 heterohexamer. Interacts with multi-pass transmembrane proteins in transit.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
FUS3P168922EBI-11303,EBI-7193
TIM10P871082EBI-11303,EBI-9115

Protein-protein interaction databases

BioGridi32797. 20 interactions.
DIPiDIP-1140N.
IntActiP32830. 5 interactions.
MINTiMINT-522035.
STRINGi4932.YBR091C.

Structurei

3D structure databases

ProteinModelPortaliP32830.
SMRiP32830. Positions 22-69.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 6627Twin CX3C motifAdd
BLAST

Domaini

The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIM12 from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane By similarity.By similarity

Sequence similaritiesi

Belongs to the small Tim family.Curated

Phylogenomic databases

eggNOGiNOG291835.
HOGENOMiHOG000211421.
InParanoidiP32830.
KOiK17793.
OMAiGEQECID.
OrthoDBiEOG7VHT9C.

Family and domain databases

Gene3Di1.10.287.810. 1 hit.
InterProiIPR004217. Tim10/DDP_fam_Znf.
IPR027247. Tim10/Tim12.
IPR027102. Tim12.
[Graphical view]
PANTHERiPTHR11038. PTHR11038. 1 hit.
PTHR11038:SF15. PTHR11038:SF15. 1 hit.
PfamiPF02953. zf-Tim10_DDP. 1 hit.
[Graphical view]
SUPFAMiSSF144122. SSF144122. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32830-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSFFLNSLRG NQEVSQEKLD VAGVQFDAMC STFNNILSTC LEKCIPHEGF
60 70 80 90 100
GEPDLTKGEQ CCIDRCVAKM HYSNRLIGGF VQTRGFGPEN QLRHYSRFVA

KEIADDSKK
Length:109
Mass (Da):12,284
Last modified:October 1, 1993 - v1
Checksum:i2C4B7DEE9695866D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M90689 Genomic DNA. Translation: AAC37483.1.
X78993 Genomic DNA. Translation: CAA55596.1.
Z35960 Genomic DNA. Translation: CAA85044.1.
AY558529 Genomic DNA. Translation: AAS56855.1.
BK006936 Genomic DNA. Translation: DAA07212.1.
PIRiS30793.
RefSeqiNP_009649.1. NM_001178439.1.

Genome annotation databases

EnsemblFungiiYBR091C; YBR091C; YBR091C.
GeneIDi852388.
KEGGisce:YBR091C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M90689 Genomic DNA. Translation: AAC37483.1 .
X78993 Genomic DNA. Translation: CAA55596.1 .
Z35960 Genomic DNA. Translation: CAA85044.1 .
AY558529 Genomic DNA. Translation: AAS56855.1 .
BK006936 Genomic DNA. Translation: DAA07212.1 .
PIRi S30793.
RefSeqi NP_009649.1. NM_001178439.1.

3D structure databases

ProteinModelPortali P32830.
SMRi P32830. Positions 22-69.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32797. 20 interactions.
DIPi DIP-1140N.
IntActi P32830. 5 interactions.
MINTi MINT-522035.
STRINGi 4932.YBR091C.

Proteomic databases

MaxQBi P32830.
PaxDbi P32830.
PeptideAtlasi P32830.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR091C ; YBR091C ; YBR091C .
GeneIDi 852388.
KEGGi sce:YBR091C.

Organism-specific databases

CYGDi YBR091c.
SGDi S000000295. TIM12.

Phylogenomic databases

eggNOGi NOG291835.
HOGENOMi HOG000211421.
InParanoidi P32830.
KOi K17793.
OMAi GEQECID.
OrthoDBi EOG7VHT9C.

Enzyme and pathway databases

BioCyci YEAST:G3O-29057-MONOMER.
Reactomei REACT_189012. Mitochondrial protein import.

Miscellaneous databases

NextBioi 971201.

Gene expression databases

Genevestigatori P32830.

Family and domain databases

Gene3Di 1.10.287.810. 1 hit.
InterProi IPR004217. Tim10/DDP_fam_Znf.
IPR027247. Tim10/Tim12.
IPR027102. Tim12.
[Graphical view ]
PANTHERi PTHR11038. PTHR11038. 1 hit.
PTHR11038:SF15. PTHR11038:SF15. 1 hit.
Pfami PF02953. zf-Tim10_DDP. 1 hit.
[Graphical view ]
SUPFAMi SSF144122. SSF144122. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mrs5p, an essential protein of the mitochondrial intermembrane space, affects protein import into yeast mitochondria."
    Jarosch E., Tuller G., Daum G., Waldherr M., Voskova A., Schweyen R.J.
    J. Biol. Chem. 271:17219-17225(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
  2. "Analysis of a 70 kb region on the right arm of yeast chromosome II."
    Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.
    Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "Import of carrier proteins into the mitochondrial inner membrane mediated by Tim22."
    Sirrenberg C., Bauer M.D., Guiard B., Neupert W., Brunner M.
    Nature 384:582-585(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TIM10 AND TIM22.
  7. "Carrier protein import into mitochondria mediated by the intermembrane proteins Tim10/Mrs11 and Tim12/Mrs5."
    Sirrenberg C., Endres M., Foelsch H., Stuart R.A., Neupert W., Brunner M.
    Nature 391:912-915(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, PROBABLE ZINC-BINDING, INTERACTION WITH TIM10 AND TIM22.
  8. "Import of mitochondrial carriers mediated by essential proteins of the intermembrane space."
    Koehler C.M., Jarosch E., Tokatlidis K., Schmid K., Schweyen R.J., Schatz G.
    Science 279:369-373(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TIM10 AND TIM22.
  9. "Two intermembrane space TIM complexes interact with different domains of Tim23p during its import into mitochondria."
    Davis A.J., Sepuri N.B., Holder J., Johnson A.E., Jensen R.E.
    J. Cell Biol. 150:1271-1282(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Tim9, a new component of the TIM22.54 translocase in mitochondria."
    Adam A., Endres M., Sirrenberg C., Lottspeich F., Neupert W., Brunner M.
    EMBO J. 18:313-319(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TIM9 AND TIM10.
  11. "Transport of the ADP/ATP carrier of mitochondria from the TOM complex to the TIM22.54 complex."
    Endres M., Neupert W., Brunner M.
    EMBO J. 18:3214-3221(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "Tim18p, a new subunit of the TIM22 complex that mediates insertion of imported proteins into the yeast mitochondrial inner membrane."
    Koehler C.M., Murphy M.P., Bally N.A., Leuenberger D., Oppliger W., Dolfini L., Junne T., Schatz G., Or E.
    Mol. Cell. Biol. 20:1187-1193(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A THE TIM22 COMPLEX WITH TIM18; TIM22 AND TIM54.
  13. "Protein insertion into the mitochondrial inner membrane by a twin-pore translocase."
    Rehling P., Model K., Brandner K., Kovermann P., Sickmann A., Meyer H.E., Kuehlbrandt W., Wagner R., Truscott K.N., Pfanner N.
    Science 299:1747-1751(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE TIM22 COMPLEX WITH TIM10; TIM18; TIM22 AND TIM54.
  14. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTIM12_YEAST
AccessioniPrimary (citable) accession number: P32830
Secondary accession number(s): D6VQ92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: October 29, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3