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Protein

Mitochondrial import inner membrane translocase subunit TIM12

Gene

TIM12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. The TIM22 complex forms a twin-pore translocase that uses the membrane potential as external driving force. In the TIM22 complex, it acts as a docking point for the soluble TIM9-TIM10 heterohexamer that guides the target proteins in transit through the aqueous mitochondrial intermembrane space.5 Publications

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • phospholipid binding Source: SGD

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29057-MONOMER.
ReactomeiR-SCE-1268020. Mitochondrial protein import.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM12
Alternative name(s):
Mitochondrial regulator of splicing 5
Gene namesi
Name:TIM12
Synonyms:MRS5
Ordered Locus Names:YBR091C
ORF Names:YBR0812
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR091C.
SGDiS000000295. TIM12.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000965812 – 109Mitochondrial import inner membrane translocase subunit TIM12Add BLAST108

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Disulfide bondi40 ↔ 66By similarity
Disulfide bondi44 ↔ 62By similarity

Keywords - PTMi

Acetylation, Disulfide bond

Proteomic databases

MaxQBiP32830.
PRIDEiP32830.

Interactioni

Subunit structurei

Component of the TIM22 complex, whose core is composed of TIM18, TIM22 and TIM54, associated with the peripheral proteins MRS5/TIM12 and the 70 kDa heterohexamer composed of TIM9 and TIM10 (or TIM8 and TIM13). Interacts directly with both the TIM22 protein and the TIM9-TIM10 heterohexamer. Interacts with multi-pass transmembrane proteins in transit.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
FUS3P168922EBI-11303,EBI-7193
TIM10P871082EBI-11303,EBI-9115

Protein-protein interaction databases

BioGridi32797. 22 interactors.
DIPiDIP-1140N.
IntActiP32830. 5 interactors.
MINTiMINT-522035.

Structurei

3D structure databases

ProteinModelPortaliP32830.
SMRiP32830.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi40 – 66Twin CX3C motifAdd BLAST27

Domaini

The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIM12 from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane (By similarity).By similarity

Sequence similaritiesi

Belongs to the small Tim family.Curated

Phylogenomic databases

HOGENOMiHOG000211421.
InParanoidiP32830.
KOiK17793.
OMAiCLEKCIP.
OrthoDBiEOG092C5IPN.

Family and domain databases

Gene3Di1.10.287.810. 1 hit.
InterProiIPR004217. Tim10/DDP_fam_Znf.
IPR027247. Tim10/Tim12.
IPR027102. Tim12.
[Graphical view]
PANTHERiPTHR11038. PTHR11038. 1 hit.
PTHR11038:SF18. PTHR11038:SF18. 1 hit.
PfamiPF02953. zf-Tim10_DDP. 1 hit.
[Graphical view]
SUPFAMiSSF144122. SSF144122. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32830-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFFLNSLRG NQEVSQEKLD VAGVQFDAMC STFNNILSTC LEKCIPHEGF
60 70 80 90 100
GEPDLTKGEQ CCIDRCVAKM HYSNRLIGGF VQTRGFGPEN QLRHYSRFVA

KEIADDSKK
Length:109
Mass (Da):12,284
Last modified:October 1, 1993 - v1
Checksum:i2C4B7DEE9695866D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90689 Genomic DNA. Translation: AAC37483.1.
X78993 Genomic DNA. Translation: CAA55596.1.
Z35960 Genomic DNA. Translation: CAA85044.1.
AY558529 Genomic DNA. Translation: AAS56855.1.
BK006936 Genomic DNA. Translation: DAA07212.1.
PIRiS30793.
RefSeqiNP_009649.1. NM_001178439.1.

Genome annotation databases

EnsemblFungiiYBR091C; YBR091C; YBR091C.
GeneIDi852388.
KEGGisce:YBR091C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90689 Genomic DNA. Translation: AAC37483.1.
X78993 Genomic DNA. Translation: CAA55596.1.
Z35960 Genomic DNA. Translation: CAA85044.1.
AY558529 Genomic DNA. Translation: AAS56855.1.
BK006936 Genomic DNA. Translation: DAA07212.1.
PIRiS30793.
RefSeqiNP_009649.1. NM_001178439.1.

3D structure databases

ProteinModelPortaliP32830.
SMRiP32830.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32797. 22 interactors.
DIPiDIP-1140N.
IntActiP32830. 5 interactors.
MINTiMINT-522035.

Proteomic databases

MaxQBiP32830.
PRIDEiP32830.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR091C; YBR091C; YBR091C.
GeneIDi852388.
KEGGisce:YBR091C.

Organism-specific databases

EuPathDBiFungiDB:YBR091C.
SGDiS000000295. TIM12.

Phylogenomic databases

HOGENOMiHOG000211421.
InParanoidiP32830.
KOiK17793.
OMAiCLEKCIP.
OrthoDBiEOG092C5IPN.

Enzyme and pathway databases

BioCyciYEAST:G3O-29057-MONOMER.
ReactomeiR-SCE-1268020. Mitochondrial protein import.

Miscellaneous databases

PROiP32830.

Family and domain databases

Gene3Di1.10.287.810. 1 hit.
InterProiIPR004217. Tim10/DDP_fam_Znf.
IPR027247. Tim10/Tim12.
IPR027102. Tim12.
[Graphical view]
PANTHERiPTHR11038. PTHR11038. 1 hit.
PTHR11038:SF18. PTHR11038:SF18. 1 hit.
PfamiPF02953. zf-Tim10_DDP. 1 hit.
[Graphical view]
SUPFAMiSSF144122. SSF144122. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTIM12_YEAST
AccessioniPrimary (citable) accession number: P32830
Secondary accession number(s): D6VQ92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 2, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.