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P32830

- TIM12_YEAST

UniProt

P32830 - TIM12_YEAST

Protein

Mitochondrial import inner membrane translocase subunit TIM12

Gene

TIM12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Essential component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. The TIM22 complex forms a twin-pore translocase that uses the membrane potential as external driving force. In the TIM22 complex, it acts as a docking point for the soluble TIM9-TIM10 heterohexamer that guides the target proteins in transit through the aqueous mitochondrial intermembrane space.5 Publications

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. phospholipid binding Source: SGD
    3. protein binding Source: IntAct

    GO - Biological processi

    1. protein import into mitochondrial inner membrane Source: SGD

    Keywords - Biological processi

    Protein transport, Translocation, Transport

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29057-MONOMER.
    ReactomeiREACT_189012. Mitochondrial protein import.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitochondrial import inner membrane translocase subunit TIM12
    Alternative name(s):
    Mitochondrial regulator of splicing 5
    Gene namesi
    Name:TIM12
    Synonyms:MRS5
    Ordered Locus Names:YBR091C
    ORF Names:YBR0812
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR091c.
    SGDiS000000295. TIM12.

    Subcellular locationi

    Mitochondrion inner membrane 2 Publications; Peripheral membrane protein 2 Publications

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: Reactome
    2. mitochondrial inner membrane protein insertion complex Source: SGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 109108Mitochondrial import inner membrane translocase subunit TIM12PRO_0000096581Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Disulfide bondi40 ↔ 66By similarity
    Disulfide bondi44 ↔ 62By similarity

    Keywords - PTMi

    Acetylation, Disulfide bond

    Proteomic databases

    MaxQBiP32830.
    PaxDbiP32830.
    PeptideAtlasiP32830.

    Expressioni

    Gene expression databases

    GenevestigatoriP32830.

    Interactioni

    Subunit structurei

    Component of the TIM22 complex, whose core is composed of TIM18, TIM22 and TIM54, associated with the peripheral proteins MRS5/TIM12 and the 70 kDa heterohexamer composed of TIM9 and TIM10 (or TIM8 and TIM13). Interacts directly with both the TIM22 protein and the TIM9-TIM10 heterohexamer. Interacts with multi-pass transmembrane proteins in transit.6 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    FUS3P168922EBI-11303,EBI-7193
    TIM10P871082EBI-11303,EBI-9115

    Protein-protein interaction databases

    BioGridi32797. 20 interactions.
    DIPiDIP-1140N.
    IntActiP32830. 5 interactions.
    MINTiMINT-522035.
    STRINGi4932.YBR091C.

    Structurei

    3D structure databases

    ProteinModelPortaliP32830.
    SMRiP32830. Positions 22-69.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi40 – 6627Twin CX3C motifAdd
    BLAST

    Domaini

    The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIM12 from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane By similarity.By similarity

    Sequence similaritiesi

    Belongs to the small Tim family.Curated

    Phylogenomic databases

    eggNOGiNOG291835.
    HOGENOMiHOG000211421.
    KOiK17793.
    OMAiGEQECID.
    OrthoDBiEOG7VHT9C.

    Family and domain databases

    Gene3Di1.10.287.810. 1 hit.
    InterProiIPR004217. Tim10/DDP_fam_Znf.
    IPR027247. Tim10/Tim12.
    IPR027102. Tim12.
    [Graphical view]
    PANTHERiPTHR11038. PTHR11038. 1 hit.
    PTHR11038:SF15. PTHR11038:SF15. 1 hit.
    PfamiPF02953. zf-Tim10_DDP. 1 hit.
    [Graphical view]
    SUPFAMiSSF144122. SSF144122. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P32830-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSFFLNSLRG NQEVSQEKLD VAGVQFDAMC STFNNILSTC LEKCIPHEGF    50
    GEPDLTKGEQ CCIDRCVAKM HYSNRLIGGF VQTRGFGPEN QLRHYSRFVA 100
    KEIADDSKK 109
    Length:109
    Mass (Da):12,284
    Last modified:October 1, 1993 - v1
    Checksum:i2C4B7DEE9695866D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M90689 Genomic DNA. Translation: AAC37483.1.
    X78993 Genomic DNA. Translation: CAA55596.1.
    Z35960 Genomic DNA. Translation: CAA85044.1.
    AY558529 Genomic DNA. Translation: AAS56855.1.
    BK006936 Genomic DNA. Translation: DAA07212.1.
    PIRiS30793.
    RefSeqiNP_009649.1. NM_001178439.1.

    Genome annotation databases

    EnsemblFungiiYBR091C; YBR091C; YBR091C.
    GeneIDi852388.
    KEGGisce:YBR091C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M90689 Genomic DNA. Translation: AAC37483.1 .
    X78993 Genomic DNA. Translation: CAA55596.1 .
    Z35960 Genomic DNA. Translation: CAA85044.1 .
    AY558529 Genomic DNA. Translation: AAS56855.1 .
    BK006936 Genomic DNA. Translation: DAA07212.1 .
    PIRi S30793.
    RefSeqi NP_009649.1. NM_001178439.1.

    3D structure databases

    ProteinModelPortali P32830.
    SMRi P32830. Positions 22-69.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32797. 20 interactions.
    DIPi DIP-1140N.
    IntActi P32830. 5 interactions.
    MINTi MINT-522035.
    STRINGi 4932.YBR091C.

    Proteomic databases

    MaxQBi P32830.
    PaxDbi P32830.
    PeptideAtlasi P32830.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR091C ; YBR091C ; YBR091C .
    GeneIDi 852388.
    KEGGi sce:YBR091C.

    Organism-specific databases

    CYGDi YBR091c.
    SGDi S000000295. TIM12.

    Phylogenomic databases

    eggNOGi NOG291835.
    HOGENOMi HOG000211421.
    KOi K17793.
    OMAi GEQECID.
    OrthoDBi EOG7VHT9C.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29057-MONOMER.
    Reactomei REACT_189012. Mitochondrial protein import.

    Miscellaneous databases

    NextBioi 971201.

    Gene expression databases

    Genevestigatori P32830.

    Family and domain databases

    Gene3Di 1.10.287.810. 1 hit.
    InterProi IPR004217. Tim10/DDP_fam_Znf.
    IPR027247. Tim10/Tim12.
    IPR027102. Tim12.
    [Graphical view ]
    PANTHERi PTHR11038. PTHR11038. 1 hit.
    PTHR11038:SF15. PTHR11038:SF15. 1 hit.
    Pfami PF02953. zf-Tim10_DDP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF144122. SSF144122. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Mrs5p, an essential protein of the mitochondrial intermembrane space, affects protein import into yeast mitochondria."
      Jarosch E., Tuller G., Daum G., Waldherr M., Voskova A., Schweyen R.J.
      J. Biol. Chem. 271:17219-17225(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
    2. "Analysis of a 70 kb region on the right arm of yeast chromosome II."
      Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.
      Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "Import of carrier proteins into the mitochondrial inner membrane mediated by Tim22."
      Sirrenberg C., Bauer M.D., Guiard B., Neupert W., Brunner M.
      Nature 384:582-585(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TIM10 AND TIM22.
    7. "Carrier protein import into mitochondria mediated by the intermembrane proteins Tim10/Mrs11 and Tim12/Mrs5."
      Sirrenberg C., Endres M., Foelsch H., Stuart R.A., Neupert W., Brunner M.
      Nature 391:912-915(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, PROBABLE ZINC-BINDING, INTERACTION WITH TIM10 AND TIM22.
    8. "Import of mitochondrial carriers mediated by essential proteins of the intermembrane space."
      Koehler C.M., Jarosch E., Tokatlidis K., Schmid K., Schweyen R.J., Schatz G.
      Science 279:369-373(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH TIM10 AND TIM22.
    9. "Two intermembrane space TIM complexes interact with different domains of Tim23p during its import into mitochondria."
      Davis A.J., Sepuri N.B., Holder J., Johnson A.E., Jensen R.E.
      J. Cell Biol. 150:1271-1282(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "Tim9, a new component of the TIM22.54 translocase in mitochondria."
      Adam A., Endres M., Sirrenberg C., Lottspeich F., Neupert W., Brunner M.
      EMBO J. 18:313-319(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TIM9 AND TIM10.
    11. "Transport of the ADP/ATP carrier of mitochondria from the TOM complex to the TIM22.54 complex."
      Endres M., Neupert W., Brunner M.
      EMBO J. 18:3214-3221(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. "Tim18p, a new subunit of the TIM22 complex that mediates insertion of imported proteins into the yeast mitochondrial inner membrane."
      Koehler C.M., Murphy M.P., Bally N.A., Leuenberger D., Oppliger W., Dolfini L., Junne T., Schatz G., Or E.
      Mol. Cell. Biol. 20:1187-1193(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A THE TIM22 COMPLEX WITH TIM18; TIM22 AND TIM54.
    13. "Protein insertion into the mitochondrial inner membrane by a twin-pore translocase."
      Rehling P., Model K., Brandner K., Kovermann P., Sickmann A., Meyer H.E., Kuehlbrandt W., Wagner R., Truscott K.N., Pfanner N.
      Science 299:1747-1751(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE TIM22 COMPLEX WITH TIM10; TIM18; TIM22 AND TIM54.
    14. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTIM12_YEAST
    AccessioniPrimary (citable) accession number: P32830
    Secondary accession number(s): D6VQ92
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3