P32830 (TIM12_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial import inner membrane translocase subunit TIM12

Alternative name(s):
Mitochondrial regulator of splicing 5

Gene names

Name:	TIM12
Synonyms:	MRS5
Ordered Locus Names:	YBR091C
ORF Names:	YBR0812

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	109 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. The TIM22 complex forms a twin-pore translocase that uses the membrane potential as external driving force. In the TIM22 complex, it acts as a docking point for the soluble TIM9-TIM10 heterohexamer that guides the target proteins in transit through the aqueous mitochondrial intermembrane space. Ref.1 Ref.7 Ref.8 Ref.9 Ref.11
Subunit structure	Component of the TIM22 complex, whose core is composed of TIM18, TIM22 and TIM54, associated with the peripheral proteins MRS5/TIM12 and the 70 kDa heterohexamer composed of TIM9 and TIM10 (or TIM8 and TIM13). Interacts directly with both the TIM22 protein and the TIM9-TIM10 heterohexamer. Interacts with multi-pass transmembrane proteins in transit. Ref.6 Ref.7 Ref.8 Ref.10
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein Ref.1 Ref.7.
Domain	The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIM12 from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane By similarity.
Sequence similarities	Belongs to the small Tim family.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Ligand	Metal-binding Zinc
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein import into mitochondrial inner membrane Inferred from mutant phenotype Ref.8. Source: SGD
Cellular component	mitochondrial inner membrane protein insertion complex Inferred from direct assay Ref.7. Source: SGD mitochondrial intermembrane space protein transporter complex Inferred from electronic annotation. Source: InterPro
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipid binding Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 109	109	Mitochondrial import inner membrane translocase subunit TIM12		PRO_0000096581
Regions
	Motif	40 – 66	27	Twin CX3C motif
Amino acid modifications
	Disulfide bond	40 ↔ 66		By similarity
	Disulfide bond	44 ↔ 62		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P32830 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 2C4B7DEE9695866D	FASTA	109	12,284
	10 20 30 40 50 60 MSFFLNSLRG NQEVSQEKLD VAGVQFDAMC STFNNILSTC LEKCIPHEGF GEPDLTKGEQ 70 80 90 100 CCIDRCVAKM HYSNRLIGGF VQTRGFGPEN QLRHYSRFVA KEIADDSKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mrs5p, an essential protein of the mitochondrial intermembrane space, affects protein import into yeast mitochondria." Jarosch E., Tuller G., Daum G., Waldherr M., Voskova A., Schweyen R.J. J. Biol. Chem. 271:17219-17225(1996) [PubMed: 8663351] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
[2]	"Analysis of a 70 kb region on the right arm of yeast chromosome II." Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H. Yeast 10:1363-1381(1994) [PubMed: 7900426] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Import of carrier proteins into the mitochondrial inner membrane mediated by Tim22." Sirrenberg C., Bauer M.D., Guiard B., Neupert W., Brunner M. Nature 384:582-585(1996) [PubMed: 8955274] [Abstract] Cited for: INTERACTION WITH TIM10 AND TIM22.
[7]	"Carrier protein import into mitochondria mediated by the intermembrane proteins Tim10/Mrs11 and Tim12/Mrs5." Sirrenberg C., Endres M., Foelsch H., Stuart R.A., Neupert W., Brunner M. Nature 391:912-915(1998) [PubMed: 9495346] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, PROBABLE ZINC-BINDING, INTERACTION WITH TIM10 AND TIM22.
[8]	"Import of mitochondrial carriers mediated by essential proteins of the intermembrane space." Koehler C.M., Jarosch E., Tokatlidis K., Schmid K., Schweyen R.J., Schatz G. Science 279:369-373(1998) [PubMed: 9430585] [Abstract] Cited for: FUNCTION, INTERACTION WITH TIM10 AND TIM22.
[9]	"Two intermembrane space TIM complexes interact with different domains of Tim23p during its import into mitochondria." Davis A.J., Sepuri N.B., Holder J., Johnson A.E., Jensen R.E. J. Cell Biol. 150:1271-1282(2000) [PubMed: 10995434] [Abstract] Cited for: FUNCTION.
[10]	"Tim9, a new component of the TIM22.54 translocase in mitochondria." Adam A., Endres M., Sirrenberg C., Lottspeich F., Neupert W., Brunner M. EMBO J. 18:313-319(1999) [PubMed: 9889188] [Abstract] Cited for: INTERACTION WITH TIM9 AND TIM10.
[11]	"Transport of the ADP/ATP carrier of mitochondria from the TOM complex to the TIM22.54 complex." Endres M., Neupert W., Brunner M. EMBO J. 18:3214-3221(1999) [PubMed: 10369662] [Abstract] Cited for: FUNCTION.
[12]	"Tim18p, a new subunit of the TIM22 complex that mediates insertion of imported proteins into the yeast mitochondrial inner membrane." Koehler C.M., Murphy M.P., Bally N.A., Leuenberger D., Oppliger W., Dolfini L., Junne T., Schatz G., Or E. Mol. Cell. Biol. 20:1187-1193(2000) [PubMed: 10648604] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A THE TIM22 COMPLEX WITH TIM18; TIM22 AND TIM54.
[13]	"Protein insertion into the mitochondrial inner membrane by a twin-pore translocase." Rehling P., Model K., Brandner K., Kovermann P., Sickmann A., Meyer H.E., Kuehlbrandt W., Wagner R., Truscott K.N., Pfanner N. Science 299:1747-1751(2003) [PubMed: 12637749] [Abstract] Cited for: IDENTIFICATION IN THE TIM22 COMPLEX WITH TIM10; TIM18; TIM22 AND TIM54.
[14]	Erratum Rehling P., Model K., Brandner K., Kovermann P., Sickmann A., Meyer H.E., Kuehlbrandt W., Wagner R., Truscott K.N., Pfanner N. Science 300:251-251(2003)
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M90689 Genomic DNA. Translation: AAC37483.1. X78993 Genomic DNA. Translation: CAA55596.1. Z35960 Genomic DNA. Translation: CAA85044.1. AY558529 Genomic DNA. Translation: AAS56855.1. BK006936 Genomic DNA. Translation: DAA07212.1.
PIR	S30793.
RefSeq	NP_009649.1. NM_001178439.1.
3D structure databases
ProteinModelPortal	P32830.
SMR	P32830. Positions 15-84.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1140N.
IntAct	P32830. 3 interactions.
MINT	MINT-522035.
STRING	P32830.
Proteomic databases
PeptideAtlas	P32830.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR091C; YBR091C; YBR091C.
GeneID	852388.
KEGG	sce:YBR091C.
NMPDR	fig|4932.3.peg.348.
Organism-specific databases
CYGD	YBR091c.
SGD	S000000295. TIM12.
Phylogenomic databases
eggNOG	fuNOG10885.
HOGENOM	HBG560646.
OMA	CLEKCIP.
OrthoDB	EOG4PZNHC.
Gene expression databases
ArrayExpress	P32830.
Genevestigator	P32830.
GermOnline	YBR091C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004217. Tim8/9/10/13_Znf-like. [Graphical view]
Gene3D	G3DSA:1.10.287.810. G3DSA:1.10.287.810. 1 hit.
Pfam	PF02953. zf-Tim10_DDP. 1 hit. [Graphical view]
SUPFAM	SSF144122. SSF144122. 1 hit.
ProtoNet	Search...
Other
NextBio	971201.

Entry information

Entry name

TIM12_YEAST

Accession

Primary (citable) accession number: P32830
Secondary accession number(s): D6VQ92

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	January 25, 2012
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program