Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P32829

- MRE11_YEAST

UniProt

P32829 - MRE11_YEAST

Protein

Double-strand break repair protein MRE11

Gene

MRE11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 146 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in DNA double-strand break repair (DSBR). Possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity. Also involved in meiotic DSB processing.2 Publications

    Cofactori

    Manganese.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei125 – 1251Proton donorBy similarity

    GO - Molecular functioni

    1. 3'-5'-exodeoxyribonuclease activity Source: Reactome
    2. 3'-5' exonuclease activity Source: SGD
    3. double-stranded telomeric DNA binding Source: SGD
    4. endodeoxyribonuclease activity Source: SGD
    5. endonuclease activity Source: SGD
    6. G-quadruplex DNA binding Source: SGD
    7. manganese ion binding Source: InterPro
    8. protein binding Source: IntAct
    9. protein complex scaffold Source: SGD
    10. single-stranded telomeric DNA binding Source: SGD
    11. telomeric DNA binding Source: SGD

    GO - Biological processi

    1. ascospore formation Source: SGD
    2. base-excision repair Source: SGD
    3. DNA catabolic process, endonucleolytic Source: GOC
    4. DNA repair Source: SGD
    5. double-strand break repair via break-induced replication Source: SGD
    6. double-strand break repair via nonhomologous end joining Source: SGD
    7. meiotic DNA double-strand break formation Source: SGD
    8. meiotic DNA double-strand break processing Source: SGD
    9. mitochondrial double-strand break repair via homologous recombination Source: SGD
    10. nucleotide phosphorylation Source: GOC
    11. reciprocal meiotic recombination Source: SGD
    12. regulation of transcription during meiosis Source: SGD

    Keywords - Molecular functioni

    Endonuclease, Exonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA repair, Meiosis

    Keywords - Ligandi

    Manganese

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32905-MONOMER.
    ReactomeiREACT_189186. Meiotic recombination.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Double-strand break repair protein MRE11
    Gene namesi
    Name:MRE11
    Ordered Locus Names:YMR224C
    ORF Names:YM9959.06C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR224c.
    SGDiS000004837. MRE11.

    Subcellular locationi

    GO - Cellular componenti

    1. Mre11 complex Source: SGD
    2. nucleoplasm Source: Reactome
    3. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 692692Double-strand break repair protein MRE11PRO_0000138680Add
    BLAST

    Proteomic databases

    MaxQBiP32829.
    PaxDbiP32829.

    Expressioni

    Gene expression databases

    GenevestigatoriP32829.

    Interactioni

    Subunit structurei

    Forms a complex with RAD50 and XRS2.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RAD50P127534EBI-11255,EBI-14700
    XRS2P333016EBI-11255,EBI-20599

    Protein-protein interaction databases

    BioGridi35402. 407 interactions.
    DIPiDIP-1313N.
    IntActiP32829. 5 interactions.
    MINTiMINT-388080.

    Structurei

    3D structure databases

    ProteinModelPortaliP32829.
    SMRiP32829. Positions 7-412.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the MRE11/RAD32 family.Curated

    Phylogenomic databases

    eggNOGiCOG0420.
    GeneTreeiENSGT00390000017288.
    HOGENOMiHOG000216581.
    KOiK10865.
    OMAiQVTMLRP.
    OrthoDBiEOG7J187V.

    Family and domain databases

    Gene3Di3.60.21.10. 2 hits.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR003701. DNA_repair_Mre11.
    IPR029052. Metallo-depent_PP-like.
    IPR007281. Mre11_DNA-bd.
    [Graphical view]
    PANTHERiPTHR10139. PTHR10139. 1 hit.
    PfamiPF00149. Metallophos. 1 hit.
    PF04152. Mre11_DNA_bind. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000882. DSB_repair_MRE11. 1 hit.
    SUPFAMiSSF56300. SSF56300. 2 hits.
    TIGRFAMsiTIGR00583. mre11. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P32829-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYPDPDTIR ILITTDNHVG YNENDPITGD DSWKTFHEVM MLAKNNNVDM    50
    VVQSGDLFHV NKPSKKSLYQ VLKTLRLCCM GDKPCELELL SDPSQVFHYD 100
    EFTNVNYEDP NFNISIPVFG ISGNHDDASG DSLLCPMDIL HATGLINHFG 150
    KVIESDKIKV VPLLFQKGST KLALYGLAAV RDERLFRTFK DGGVTFEVPT 200
    MREGEWFNLM CVHQNHTGHT NTAFLPEQFL PDFLDMVIWG HEHECIPNLV 250
    HNPIKNFDVL QPGSSVATSL CEAEAQPKYV FILDIKYGEA PKMTPIPLET 300
    IRTFKMKSIS LQDVPHLRPH DKDATSKYLI EQVEEMIRDA NEETKQKLAD 350
    DGEGDMVAEL PKPLIRLRVD YSAPSNTQSP IDYQVENPRR FSNRFVGRVA 400
    NGNNVVQFYK KRSPVTRSKK SGINGTSISD RDVEKLFSES GGELEVQTLV 450
    NDLLNKMQLS LLPEVGLNEA VKKFVDKDEK TALKEFISHE ISNEVGILST 500
    NEEFLRTDDA EEMKALIKQV KRANSVRPTP PKENDETNFA FNGNGLDSFR 550
    SSNREVRTGS PDITQSHVDN ESRITHISQA ESSKPTSKPK RVRTATKKKI 600
    PAFSDSTVIS DAENELGDNN DAQDDVDIDE NDIIMVSTDE EDASYGLLNG 650
    RKTKTKTRPA ASTKTASRRG KGRASRTPKT DILGSLLAKK RK 692
    Length:692
    Mass (Da):77,650
    Last modified:October 1, 1996 - v2
    Checksum:iD58433E32E852B73
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti380 – 3801P → S in strain: SK1.
    Natural varianti659 – 6591P → S in strain: SK1.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D11463 Genomic DNA. Translation: BAA02017.1.
    Z49939 Genomic DNA. Translation: CAA90195.1.
    U60829 Genomic DNA. Translation: AAB61454.1.
    BK006946 Genomic DNA. Translation: DAA10123.1.
    PIRiS57592.
    RefSeqiNP_013951.1. NM_001182731.1.

    Genome annotation databases

    EnsemblFungiiYMR224C; YMR224C; YMR224C.
    GeneIDi855264.
    KEGGisce:YMR224C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D11463 Genomic DNA. Translation: BAA02017.1 .
    Z49939 Genomic DNA. Translation: CAA90195.1 .
    U60829 Genomic DNA. Translation: AAB61454.1 .
    BK006946 Genomic DNA. Translation: DAA10123.1 .
    PIRi S57592.
    RefSeqi NP_013951.1. NM_001182731.1.

    3D structure databases

    ProteinModelPortali P32829.
    SMRi P32829. Positions 7-412.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35402. 407 interactions.
    DIPi DIP-1313N.
    IntActi P32829. 5 interactions.
    MINTi MINT-388080.

    Proteomic databases

    MaxQBi P32829.
    PaxDbi P32829.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR224C ; YMR224C ; YMR224C .
    GeneIDi 855264.
    KEGGi sce:YMR224C.

    Organism-specific databases

    CYGDi YMR224c.
    SGDi S000004837. MRE11.

    Phylogenomic databases

    eggNOGi COG0420.
    GeneTreei ENSGT00390000017288.
    HOGENOMi HOG000216581.
    KOi K10865.
    OMAi QVTMLRP.
    OrthoDBi EOG7J187V.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32905-MONOMER.
    Reactomei REACT_189186. Meiotic recombination.

    Miscellaneous databases

    NextBioi 978864.
    PROi P32829.

    Gene expression databases

    Genevestigatori P32829.

    Family and domain databases

    Gene3Di 3.60.21.10. 2 hits.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR003701. DNA_repair_Mre11.
    IPR029052. Metallo-depent_PP-like.
    IPR007281. Mre11_DNA-bd.
    [Graphical view ]
    PANTHERi PTHR10139. PTHR10139. 1 hit.
    Pfami PF00149. Metallophos. 1 hit.
    PF04152. Mre11_DNA_bind. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000882. DSB_repair_MRE11. 1 hit.
    SUPFAMi SSF56300. SSF56300. 2 hits.
    TIGRFAMsi TIGR00583. mre11. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Interaction of Mre11 and Rad50: two proteins required for DNA repair and meiosis-specific double-strand break formation in Saccharomyces cerevisiae."
      Johzuka K., Ogawa H.
      Genetics 139:1521-1532(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-643.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Recombination-dependent lethals in Yeast."
      Nairz K., Klein F.
      Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: SK1.
    5. "Functions of the yeast meiotic recombination genes, MRE11 and MRE2."
      Ogawa H., Johzuka K., Nakagawa T., Leem S.H., Hagihara A.H.
      Adv. Biophys. 31:67-76(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "The 3' to 5' exonuclease activity of Mre 11 facilitates repair of DNA double-strand breaks."
      Paull T.T., Gellert M.
      Mol. Cell 1:969-979(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMRE11_YEAST
    AccessioniPrimary (citable) accession number: P32829
    Secondary accession number(s): D6W049, P87289, Q07173
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 146 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 432 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3