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Protein

Double-strand break repair protein MRE11

Gene

MRE11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in DNA double-strand break repair (DSBR). Possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity. Also involved in meiotic DSB processing.2 Publications

Cofactori

Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei125 – 1251Proton donorBy similarity

GO - Molecular functioni

  1. 3'-5'-exodeoxyribonuclease activity Source: Reactome
  2. 3'-5' exonuclease activity Source: SGD
  3. double-stranded telomeric DNA binding Source: SGD
  4. endodeoxyribonuclease activity Source: SGD
  5. endonuclease activity Source: SGD
  6. G-quadruplex DNA binding Source: SGD
  7. manganese ion binding Source: InterPro
  8. protein complex scaffold Source: SGD
  9. single-stranded telomeric DNA binding Source: SGD
  10. telomeric DNA binding Source: SGD

GO - Biological processi

  1. ascospore formation Source: SGD
  2. base-excision repair Source: SGD
  3. DNA catabolic process, endonucleolytic Source: GOC
  4. DNA double-strand break processing involved in repair via synthesis-dependent strand annealing Source: SGD
  5. DNA repair Source: SGD
  6. double-strand break repair via break-induced replication Source: SGD
  7. double-strand break repair via nonhomologous end joining Source: SGD
  8. meiotic DNA double-strand break formation Source: SGD
  9. meiotic DNA double-strand break processing Source: SGD
  10. mitochondrial double-strand break repair via homologous recombination Source: SGD
  11. nucleotide phosphorylation Source: GOC
  12. reciprocal meiotic recombination Source: SGD
  13. regulation of transcription during meiosis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair, Meiosis

Keywords - Ligandi

Manganese

Enzyme and pathway databases

BioCyciYEAST:G3O-32905-MONOMER.
ReactomeiREACT_243677. Assembly of the RAD50-MRE11-NBS1 complex at DNA double-strand breaks.
REACT_268498. Meiotic recombination.

Names & Taxonomyi

Protein namesi
Recommended name:
Double-strand break repair protein MRE11
Gene namesi
Name:MRE11
Ordered Locus Names:YMR224C
ORF Names:YM9959.06C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR224c.
SGDiS000004837. MRE11.

Subcellular locationi

GO - Cellular componenti

  1. Mre11 complex Source: SGD
  2. nucleoplasm Source: Reactome
  3. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 692692Double-strand break repair protein MRE11PRO_0000138680Add
BLAST

Proteomic databases

MaxQBiP32829.
PaxDbiP32829.

Expressioni

Gene expression databases

GenevestigatoriP32829.

Interactioni

Subunit structurei

Forms a complex with RAD50 and XRS2.

Binary interactionsi

WithEntry#Exp.IntActNotes
RAD50P127534EBI-11255,EBI-14700
XRS2P333016EBI-11255,EBI-20599

Protein-protein interaction databases

BioGridi35402. 412 interactions.
DIPiDIP-1313N.
IntActiP32829. 5 interactions.
MINTiMINT-388080.

Structurei

3D structure databases

ProteinModelPortaliP32829.
SMRiP32829. Positions 7-412.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MRE11/RAD32 family.Curated

Phylogenomic databases

eggNOGiCOG0420.
GeneTreeiENSGT00390000017288.
HOGENOMiHOG000216581.
InParanoidiP32829.
KOiK10865.
OMAiRMFVNKQ.
OrthoDBiEOG7J187V.

Family and domain databases

Gene3Di3.60.21.10. 2 hits.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR003701. DNA_repair_Mre11.
IPR029052. Metallo-depent_PP-like.
IPR007281. Mre11_DNA-bd.
[Graphical view]
PANTHERiPTHR10139. PTHR10139. 1 hit.
PfamiPF00149. Metallophos. 1 hit.
PF04152. Mre11_DNA_bind. 1 hit.
[Graphical view]
PIRSFiPIRSF000882. DSB_repair_MRE11. 1 hit.
SUPFAMiSSF56300. SSF56300. 2 hits.
TIGRFAMsiTIGR00583. mre11. 1 hit.

Sequencei

Sequence statusi: Complete.

P32829-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYPDPDTIR ILITTDNHVG YNENDPITGD DSWKTFHEVM MLAKNNNVDM
60 70 80 90 100
VVQSGDLFHV NKPSKKSLYQ VLKTLRLCCM GDKPCELELL SDPSQVFHYD
110 120 130 140 150
EFTNVNYEDP NFNISIPVFG ISGNHDDASG DSLLCPMDIL HATGLINHFG
160 170 180 190 200
KVIESDKIKV VPLLFQKGST KLALYGLAAV RDERLFRTFK DGGVTFEVPT
210 220 230 240 250
MREGEWFNLM CVHQNHTGHT NTAFLPEQFL PDFLDMVIWG HEHECIPNLV
260 270 280 290 300
HNPIKNFDVL QPGSSVATSL CEAEAQPKYV FILDIKYGEA PKMTPIPLET
310 320 330 340 350
IRTFKMKSIS LQDVPHLRPH DKDATSKYLI EQVEEMIRDA NEETKQKLAD
360 370 380 390 400
DGEGDMVAEL PKPLIRLRVD YSAPSNTQSP IDYQVENPRR FSNRFVGRVA
410 420 430 440 450
NGNNVVQFYK KRSPVTRSKK SGINGTSISD RDVEKLFSES GGELEVQTLV
460 470 480 490 500
NDLLNKMQLS LLPEVGLNEA VKKFVDKDEK TALKEFISHE ISNEVGILST
510 520 530 540 550
NEEFLRTDDA EEMKALIKQV KRANSVRPTP PKENDETNFA FNGNGLDSFR
560 570 580 590 600
SSNREVRTGS PDITQSHVDN ESRITHISQA ESSKPTSKPK RVRTATKKKI
610 620 630 640 650
PAFSDSTVIS DAENELGDNN DAQDDVDIDE NDIIMVSTDE EDASYGLLNG
660 670 680 690
RKTKTKTRPA ASTKTASRRG KGRASRTPKT DILGSLLAKK RK
Length:692
Mass (Da):77,650
Last modified:October 1, 1996 - v2
Checksum:iD58433E32E852B73
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti380 – 3801P → S in strain: SK1.
Natural varianti659 – 6591P → S in strain: SK1.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D11463 Genomic DNA. Translation: BAA02017.1.
Z49939 Genomic DNA. Translation: CAA90195.1.
U60829 Genomic DNA. Translation: AAB61454.1.
BK006946 Genomic DNA. Translation: DAA10123.1.
PIRiS57592.
RefSeqiNP_013951.1. NM_001182731.1.

Genome annotation databases

EnsemblFungiiYMR224C; YMR224C; YMR224C.
GeneIDi855264.
KEGGisce:YMR224C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D11463 Genomic DNA. Translation: BAA02017.1.
Z49939 Genomic DNA. Translation: CAA90195.1.
U60829 Genomic DNA. Translation: AAB61454.1.
BK006946 Genomic DNA. Translation: DAA10123.1.
PIRiS57592.
RefSeqiNP_013951.1. NM_001182731.1.

3D structure databases

ProteinModelPortaliP32829.
SMRiP32829. Positions 7-412.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35402. 412 interactions.
DIPiDIP-1313N.
IntActiP32829. 5 interactions.
MINTiMINT-388080.

Proteomic databases

MaxQBiP32829.
PaxDbiP32829.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR224C; YMR224C; YMR224C.
GeneIDi855264.
KEGGisce:YMR224C.

Organism-specific databases

CYGDiYMR224c.
SGDiS000004837. MRE11.

Phylogenomic databases

eggNOGiCOG0420.
GeneTreeiENSGT00390000017288.
HOGENOMiHOG000216581.
InParanoidiP32829.
KOiK10865.
OMAiRMFVNKQ.
OrthoDBiEOG7J187V.

Enzyme and pathway databases

BioCyciYEAST:G3O-32905-MONOMER.
ReactomeiREACT_243677. Assembly of the RAD50-MRE11-NBS1 complex at DNA double-strand breaks.
REACT_268498. Meiotic recombination.

Miscellaneous databases

NextBioi978864.
PROiP32829.

Gene expression databases

GenevestigatoriP32829.

Family and domain databases

Gene3Di3.60.21.10. 2 hits.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR003701. DNA_repair_Mre11.
IPR029052. Metallo-depent_PP-like.
IPR007281. Mre11_DNA-bd.
[Graphical view]
PANTHERiPTHR10139. PTHR10139. 1 hit.
PfamiPF00149. Metallophos. 1 hit.
PF04152. Mre11_DNA_bind. 1 hit.
[Graphical view]
PIRSFiPIRSF000882. DSB_repair_MRE11. 1 hit.
SUPFAMiSSF56300. SSF56300. 2 hits.
TIGRFAMsiTIGR00583. mre11. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Interaction of Mre11 and Rad50: two proteins required for DNA repair and meiosis-specific double-strand break formation in Saccharomyces cerevisiae."
    Johzuka K., Ogawa H.
    Genetics 139:1521-1532(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-643.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Recombination-dependent lethals in Yeast."
    Nairz K., Klein F.
    Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SK1.
  5. "Functions of the yeast meiotic recombination genes, MRE11 and MRE2."
    Ogawa H., Johzuka K., Nakagawa T., Leem S.H., Hagihara A.H.
    Adv. Biophys. 31:67-76(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "The 3' to 5' exonuclease activity of Mre 11 facilitates repair of DNA double-strand breaks."
    Paull T.T., Gellert M.
    Mol. Cell 1:969-979(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMRE11_YEAST
AccessioniPrimary (citable) accession number: P32829
Secondary accession number(s): D6W049, P87289, Q07173
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1996
Last modified: February 4, 2015
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 432 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.