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P32829 (MRE11_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 144. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Double-strand break repair protein MRE11
Gene names
Name:MRE11
Ordered Locus Names:YMR224C
ORF Names:YM9959.06C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length692 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in DNA double-strand break repair (DSBR). Possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity. Also involved in meiotic DSB processing. Ref.5 Ref.6

Cofactor

Manganese By similarity.

Subunit structure

Forms a complex with RAD50 and XRS2.

Subcellular location

Nucleus.

Miscellaneous

Present with 432 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the MRE11/RAD32 family.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
Meiosis
   Cellular componentNucleus
   LigandManganese
   Molecular functionEndonuclease
Exonuclease
Hydrolase
Nuclease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA catabolic process, endonucleolytic

Inferred from direct assay PubMed 16116037. Source: GOC

DNA repair

Inferred from mutant phenotype Ref.1. Source: SGD

ascospore formation

Inferred from mutant phenotype PubMed 17396017. Source: SGD

base-excision repair

Inferred from mutant phenotype PubMed 20040573. Source: SGD

double-strand break repair via break-induced replication

Inferred from mutant phenotype PubMed 17321803. Source: SGD

double-strand break repair via nonhomologous end joining

Inferred from mutant phenotype PubMed 12399380. Source: SGD

meiotic DNA double-strand break formation

Traceable author statement PubMed 9334324. Source: SGD

meiotic DNA double-strand break processing

Traceable author statement PubMed 9334324. Source: SGD

mitochondrial double-strand break repair via homologous recombination

Inferred from mutant phenotype PubMed 22214610. Source: SGD

nucleotide phosphorylation

Inferred from direct assay PubMed 17349953. Source: GOC

reciprocal meiotic recombination

Inferred from mutant phenotype PubMed 8417989. Source: SGD

regulation of transcription during meiosis

Inferred from mutant phenotype PubMed 17396017. Source: SGD

   Cellular_componentMre11 complex

Inferred from physical interaction PubMed 9845372. Source: SGD

nucleoplasm

Traceable author statement. Source: Reactome

nucleus

Inferred from direct assay PubMed 15548595PubMed 9845372. Source: SGD

   Molecular_function3'-5' exonuclease activity

Inferred from direct assay PubMed 11454871. Source: SGD

3'-5'-exodeoxyribonuclease activity

Inferred from experiment. Source: Reactome

G-quadruplex DNA binding

Inferred from direct assay PubMed 17698079. Source: SGD

double-stranded telomeric DNA binding

Inferred from direct assay PubMed 17698079. Source: SGD

endodeoxyribonuclease activity

Inferred from direct assay PubMed 16116037. Source: SGD

endonuclease activity

Inferred from direct assay PubMed 9858579. Source: SGD

manganese ion binding

Inferred from electronic annotation. Source: InterPro

protein binding

Inferred from physical interaction PubMed 23267104PubMed 9845372. Source: IntAct

protein complex scaffold

Inferred from mutant phenotype PubMed 9845372. Source: SGD

single-stranded telomeric DNA binding

Inferred from direct assay PubMed 17698079. Source: SGD

telomeric DNA binding

Inferred from direct assay PubMed 15721260PubMed 16116037. Source: SGD

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

RAD50P127534EBI-11255,EBI-14700
XRS2P333016EBI-11255,EBI-20599

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 692692Double-strand break repair protein MRE11
PRO_0000138680

Sites

Active site1251Proton donor By similarity

Natural variations

Natural variant3801P → S in strain: SK1.
Natural variant6591P → S in strain: SK1.

Sequences

Sequence LengthMass (Da)Tools
P32829 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: D58433E32E852B73

FASTA69277,650
        10         20         30         40         50         60 
MDYPDPDTIR ILITTDNHVG YNENDPITGD DSWKTFHEVM MLAKNNNVDM VVQSGDLFHV 

        70         80         90        100        110        120 
NKPSKKSLYQ VLKTLRLCCM GDKPCELELL SDPSQVFHYD EFTNVNYEDP NFNISIPVFG 

       130        140        150        160        170        180 
ISGNHDDASG DSLLCPMDIL HATGLINHFG KVIESDKIKV VPLLFQKGST KLALYGLAAV 

       190        200        210        220        230        240 
RDERLFRTFK DGGVTFEVPT MREGEWFNLM CVHQNHTGHT NTAFLPEQFL PDFLDMVIWG 

       250        260        270        280        290        300 
HEHECIPNLV HNPIKNFDVL QPGSSVATSL CEAEAQPKYV FILDIKYGEA PKMTPIPLET 

       310        320        330        340        350        360 
IRTFKMKSIS LQDVPHLRPH DKDATSKYLI EQVEEMIRDA NEETKQKLAD DGEGDMVAEL 

       370        380        390        400        410        420 
PKPLIRLRVD YSAPSNTQSP IDYQVENPRR FSNRFVGRVA NGNNVVQFYK KRSPVTRSKK 

       430        440        450        460        470        480 
SGINGTSISD RDVEKLFSES GGELEVQTLV NDLLNKMQLS LLPEVGLNEA VKKFVDKDEK 

       490        500        510        520        530        540 
TALKEFISHE ISNEVGILST NEEFLRTDDA EEMKALIKQV KRANSVRPTP PKENDETNFA 

       550        560        570        580        590        600 
FNGNGLDSFR SSNREVRTGS PDITQSHVDN ESRITHISQA ESSKPTSKPK RVRTATKKKI 

       610        620        630        640        650        660 
PAFSDSTVIS DAENELGDNN DAQDDVDIDE NDIIMVSTDE EDASYGLLNG RKTKTKTRPA 

       670        680        690 
ASTKTASRRG KGRASRTPKT DILGSLLAKK RK 

« Hide

References

« Hide 'large scale' references
[1]"Interaction of Mre11 and Rad50: two proteins required for DNA repair and meiosis-specific double-strand break formation in Saccharomyces cerevisiae."
Johzuka K., Ogawa H.
Genetics 139:1521-1532(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-643.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Recombination-dependent lethals in Yeast."
Nairz K., Klein F.
Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: SK1.
[5]"Functions of the yeast meiotic recombination genes, MRE11 and MRE2."
Ogawa H., Johzuka K., Nakagawa T., Leem S.H., Hagihara A.H.
Adv. Biophys. 31:67-76(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"The 3' to 5' exonuclease activity of Mre 11 facilitates repair of DNA double-strand breaks."
Paull T.T., Gellert M.
Mol. Cell 1:969-979(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D11463 Genomic DNA. Translation: BAA02017.1.
Z49939 Genomic DNA. Translation: CAA90195.1.
U60829 Genomic DNA. Translation: AAB61454.1.
BK006946 Genomic DNA. Translation: DAA10123.1.
PIRS57592.
RefSeqNP_013951.1. NM_001182731.1.

3D structure databases

ProteinModelPortalP32829.
SMRP32829. Positions 7-412.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35402. 405 interactions.
DIPDIP-1313N.
IntActP32829. 5 interactions.
MINTMINT-388080.

Proteomic databases

MaxQBP32829.
PaxDbP32829.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR224C; YMR224C; YMR224C.
GeneID855264.
KEGGsce:YMR224C.

Organism-specific databases

CYGDYMR224c.
SGDS000004837. MRE11.

Phylogenomic databases

eggNOGCOG0420.
GeneTreeENSGT00390000017288.
HOGENOMHOG000216581.
KOK10865.
OMAQVTMLRP.
OrthoDBEOG7J187V.

Enzyme and pathway databases

BioCycYEAST:G3O-32905-MONOMER.
ReactomeREACT_190740. Cell Cycle.

Gene expression databases

GenevestigatorP32829.

Family and domain databases

Gene3D3.60.21.10. 2 hits.
InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR003701. DNA_repair_Mre11.
IPR029052. Metallo-depent_PP-like.
IPR007281. Mre11_DNA-bd.
[Graphical view]
PANTHERPTHR10139. PTHR10139. 1 hit.
PfamPF00149. Metallophos. 1 hit.
PF04152. Mre11_DNA_bind. 1 hit.
[Graphical view]
PIRSFPIRSF000882. DSB_repair_MRE11. 1 hit.
SUPFAMSSF56300. SSF56300. 2 hits.
TIGRFAMsTIGR00583. mre11. 1 hit.
ProtoNetSearch...

Other

NextBio978864.
PROP32829.

Entry information

Entry nameMRE11_YEAST
AccessionPrimary (citable) accession number: P32829
Secondary accession number(s): D6W049, P87289, Q07173
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families