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Protein

Double-strand break repair protein MRE11

Gene

MRE11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in DNA double-strand break repair (DSBR). Possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity. Also involved in meiotic DSB processing.2 Publications

Miscellaneous

Present with 432 molecules/cell in log phase SD medium.1 Publication

Cofactori

Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei125Proton donorBy similarity1

GO - Molecular functioni

  • 3'-5'-exodeoxyribonuclease activity Source: CACAO
  • 3'-5' exonuclease activity Source: SGD
  • double-stranded telomeric DNA binding Source: SGD
  • endodeoxyribonuclease activity Source: SGD
  • endonuclease activity Source: SGD
  • G-quadruplex DNA binding Source: SGD
  • manganese ion binding Source: InterPro
  • protein complex scaffold activity Source: SGD
  • single-stranded telomeric DNA binding Source: SGD
  • telomeric DNA binding Source: SGD

GO - Biological processi

  • ascospore formation Source: SGD
  • base-excision repair Source: SGD
  • DNA double-strand break processing involved in repair via synthesis-dependent strand annealing Source: SGD
  • DNA repair Source: SGD
  • double-strand break repair Source: CACAO
  • double-strand break repair via break-induced replication Source: SGD
  • double-strand break repair via nonhomologous end joining Source: SGD
  • intra-S DNA damage checkpoint Source: GO_Central
  • maintenance of DNA trinucleotide repeats Source: SGD
  • meiotic DNA double-strand break formation Source: SGD
  • meiotic DNA double-strand break formation involved in reciprocal meiotic recombination Source: CACAO
  • meiotic DNA double-strand break processing Source: SGD
  • mitochondrial double-strand break repair via homologous recombination Source: SGD
  • nucleotide phosphorylation Source: GOC
  • reciprocal meiotic recombination Source: SGD
  • regulation of transcription involved in meiotic cell cycle Source: SGD
  • telomere maintenance Source: SGD

Keywordsi

Molecular functionEndonuclease, Exonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA repair, Meiosis
LigandManganese

Enzyme and pathway databases

BioCyciYEAST:G3O-32905-MONOMER.
ReactomeiR-SCE-1834949. Cytosolic sensors of pathogen-associated DNA.
R-SCE-2559586. DNA Damage/Telomere Stress Induced Senescence.
R-SCE-5693548. Sensing of DNA Double Strand Breaks.
R-SCE-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.
R-SCE-912446. Meiotic recombination.

Names & Taxonomyi

Protein namesi
Recommended name:
Double-strand break repair protein MRE11
Gene namesi
Name:MRE11
Ordered Locus Names:YMR224C
ORF Names:YM9959.06C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR224C.
SGDiS000004837. MRE11.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001386801 – 692Double-strand break repair protein MRE11Add BLAST692

Proteomic databases

MaxQBiP32829.
PRIDEiP32829.

PTM databases

iPTMnetiP32829.

Interactioni

Subunit structurei

Forms a complex with RAD50 and XRS2.

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein complex scaffold activity Source: SGD

Protein-protein interaction databases

BioGridi35402. 544 interactors.
DIPiDIP-1313N.
IntActiP32829. 21 interactors.
MINTiMINT-388080.
STRINGi4932.YMR224C.

Structurei

3D structure databases

ProteinModelPortaliP32829.
SMRiP32829.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MRE11/RAD32 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000017288.
HOGENOMiHOG000216581.
InParanoidiP32829.
KOiK10865.
OMAiIHFFRHR.
OrthoDBiEOG092C2IFF.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiView protein in InterPro
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR003701. Mre11.
IPR007281. Mre11_DNA-bd.
PfamiView protein in Pfam
PF00149. Metallophos. 1 hit.
PF04152. Mre11_DNA_bind. 1 hit.
PIRSFiPIRSF000882. DSB_repair_MRE11. 1 hit.
SMARTiView protein in SMART
SM01347. Mre11_DNA_bind. 1 hit.
SUPFAMiSSF56300. SSF56300. 2 hits.
TIGRFAMsiTIGR00583. mre11. 1 hit.

Sequencei

Sequence statusi: Complete.

P32829-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYPDPDTIR ILITTDNHVG YNENDPITGD DSWKTFHEVM MLAKNNNVDM
60 70 80 90 100
VVQSGDLFHV NKPSKKSLYQ VLKTLRLCCM GDKPCELELL SDPSQVFHYD
110 120 130 140 150
EFTNVNYEDP NFNISIPVFG ISGNHDDASG DSLLCPMDIL HATGLINHFG
160 170 180 190 200
KVIESDKIKV VPLLFQKGST KLALYGLAAV RDERLFRTFK DGGVTFEVPT
210 220 230 240 250
MREGEWFNLM CVHQNHTGHT NTAFLPEQFL PDFLDMVIWG HEHECIPNLV
260 270 280 290 300
HNPIKNFDVL QPGSSVATSL CEAEAQPKYV FILDIKYGEA PKMTPIPLET
310 320 330 340 350
IRTFKMKSIS LQDVPHLRPH DKDATSKYLI EQVEEMIRDA NEETKQKLAD
360 370 380 390 400
DGEGDMVAEL PKPLIRLRVD YSAPSNTQSP IDYQVENPRR FSNRFVGRVA
410 420 430 440 450
NGNNVVQFYK KRSPVTRSKK SGINGTSISD RDVEKLFSES GGELEVQTLV
460 470 480 490 500
NDLLNKMQLS LLPEVGLNEA VKKFVDKDEK TALKEFISHE ISNEVGILST
510 520 530 540 550
NEEFLRTDDA EEMKALIKQV KRANSVRPTP PKENDETNFA FNGNGLDSFR
560 570 580 590 600
SSNREVRTGS PDITQSHVDN ESRITHISQA ESSKPTSKPK RVRTATKKKI
610 620 630 640 650
PAFSDSTVIS DAENELGDNN DAQDDVDIDE NDIIMVSTDE EDASYGLLNG
660 670 680 690
RKTKTKTRPA ASTKTASRRG KGRASRTPKT DILGSLLAKK RK
Length:692
Mass (Da):77,650
Last modified:October 1, 1996 - v2
Checksum:iD58433E32E852B73
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti380P → S in strain: SK1. 1
Natural varianti659P → S in strain: SK1. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D11463 Genomic DNA. Translation: BAA02017.1.
Z49939 Genomic DNA. Translation: CAA90195.1.
U60829 Genomic DNA. Translation: AAB61454.1.
BK006946 Genomic DNA. Translation: DAA10123.1.
PIRiS57592.
RefSeqiNP_013951.1. NM_001182731.1.

Genome annotation databases

EnsemblFungiiYMR224C; YMR224C; YMR224C.
GeneIDi855264.
KEGGisce:YMR224C.

Similar proteinsi

Entry informationi

Entry nameiMRE11_YEAST
AccessioniPrimary (citable) accession number: P32829
Secondary accession number(s): D6W049, P87289, Q07173
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1996
Last modified: September 27, 2017
This is version 176 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names