Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Double-strand break repair protein MRE11

Gene

MRE11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in DNA double-strand break repair (DSBR). Possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity. Also involved in meiotic DSB processing.2 Publications

Miscellaneous

Present with 432 molecules/cell in log phase SD medium.1 Publication

Cofactori

Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei125Proton donorBy similarity1

GO - Molecular functioni

  • 3'-5'-exodeoxyribonuclease activity Source: CACAO
  • 3'-5' exonuclease activity Source: SGD
  • double-stranded telomeric DNA binding Source: SGD
  • endodeoxyribonuclease activity Source: SGD
  • endonuclease activity Source: SGD
  • G-quadruplex DNA binding Source: SGD
  • manganese ion binding Source: InterPro
  • protein-containing complex scaffold activity Source: SGD
  • single-stranded telomeric DNA binding Source: SGD
  • telomeric DNA binding Source: SGD

GO - Biological processi

  • ascospore formation Source: SGD
  • base-excision repair Source: SGD
  • DNA double-strand break processing involved in repair via synthesis-dependent strand annealing Source: SGD
  • DNA repair Source: SGD
  • double-strand break repair Source: CACAO
  • double-strand break repair via break-induced replication Source: SGD
  • double-strand break repair via nonhomologous end joining Source: SGD
  • intra-S DNA damage checkpoint Source: GO_Central
  • maintenance of DNA trinucleotide repeats Source: SGD
  • meiotic DNA double-strand break formation Source: SGD
  • meiotic DNA double-strand break formation involved in reciprocal meiotic recombination Source: CACAO
  • meiotic DNA double-strand break processing Source: SGD
  • mitochondrial double-strand break repair via homologous recombination Source: SGD
  • nucleotide phosphorylation Source: GOC
  • reciprocal meiotic recombination Source: SGD
  • regulation of transcription involved in meiotic cell cycle Source: SGD
  • telomere maintenance Source: SGD

Keywordsi

Molecular functionEndonuclease, Exonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA repair, Meiosis
LigandManganese

Enzyme and pathway databases

BioCyciYEAST:G3O-32905-MONOMER
ReactomeiR-SCE-1834949 Cytosolic sensors of pathogen-associated DNA
R-SCE-2559586 DNA Damage/Telomere Stress Induced Senescence
R-SCE-5693548 Sensing of DNA Double Strand Breaks
R-SCE-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks

Names & Taxonomyi

Protein namesi
Recommended name:
Double-strand break repair protein MRE11
Gene namesi
Name:MRE11
Ordered Locus Names:YMR224C
ORF Names:YM9959.06C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR224C
SGDiS000004837 MRE11

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001386801 – 692Double-strand break repair protein MRE11Add BLAST692

Proteomic databases

MaxQBiP32829
PaxDbiP32829
PRIDEiP32829

PTM databases

iPTMnetiP32829

Interactioni

Subunit structurei

Forms a complex with RAD50 and XRS2.

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein-containing complex scaffold activity Source: SGD

Protein-protein interaction databases

BioGridi35402, 565 interactors
DIPiDIP-1313N
IntActiP32829, 11 interactors
MINTiP32829
STRINGi4932.YMR224C

Structurei

3D structure databases

ProteinModelPortaliP32829
SMRiP32829
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MRE11/RAD32 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000017288
HOGENOMiHOG000216581
InParanoidiP32829
KOiK10865
OMAiIHFFRHR
OrthoDBiEOG092C2IFF

Family and domain databases

Gene3Di3.30.110.110, 1 hit
3.60.21.10, 1 hit
InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR029052 Metallo-depent_PP-like
IPR003701 Mre11
IPR007281 Mre11_DNA-bd
IPR038487 Mre11_DNA-bd_sf
PANTHERiPTHR10139 PTHR10139, 1 hit
PfamiView protein in Pfam
PF00149 Metallophos, 1 hit
PF04152 Mre11_DNA_bind, 1 hit
PIRSFiPIRSF000882 DSB_repair_MRE11, 1 hit
SMARTiView protein in SMART
SM01347 Mre11_DNA_bind, 1 hit
TIGRFAMsiTIGR00583 mre11, 1 hit

Sequencei

Sequence statusi: Complete.

P32829-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYPDPDTIR ILITTDNHVG YNENDPITGD DSWKTFHEVM MLAKNNNVDM
60 70 80 90 100
VVQSGDLFHV NKPSKKSLYQ VLKTLRLCCM GDKPCELELL SDPSQVFHYD
110 120 130 140 150
EFTNVNYEDP NFNISIPVFG ISGNHDDASG DSLLCPMDIL HATGLINHFG
160 170 180 190 200
KVIESDKIKV VPLLFQKGST KLALYGLAAV RDERLFRTFK DGGVTFEVPT
210 220 230 240 250
MREGEWFNLM CVHQNHTGHT NTAFLPEQFL PDFLDMVIWG HEHECIPNLV
260 270 280 290 300
HNPIKNFDVL QPGSSVATSL CEAEAQPKYV FILDIKYGEA PKMTPIPLET
310 320 330 340 350
IRTFKMKSIS LQDVPHLRPH DKDATSKYLI EQVEEMIRDA NEETKQKLAD
360 370 380 390 400
DGEGDMVAEL PKPLIRLRVD YSAPSNTQSP IDYQVENPRR FSNRFVGRVA
410 420 430 440 450
NGNNVVQFYK KRSPVTRSKK SGINGTSISD RDVEKLFSES GGELEVQTLV
460 470 480 490 500
NDLLNKMQLS LLPEVGLNEA VKKFVDKDEK TALKEFISHE ISNEVGILST
510 520 530 540 550
NEEFLRTDDA EEMKALIKQV KRANSVRPTP PKENDETNFA FNGNGLDSFR
560 570 580 590 600
SSNREVRTGS PDITQSHVDN ESRITHISQA ESSKPTSKPK RVRTATKKKI
610 620 630 640 650
PAFSDSTVIS DAENELGDNN DAQDDVDIDE NDIIMVSTDE EDASYGLLNG
660 670 680 690
RKTKTKTRPA ASTKTASRRG KGRASRTPKT DILGSLLAKK RK
Length:692
Mass (Da):77,650
Last modified:October 1, 1996 - v2
Checksum:iD58433E32E852B73
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti380P → S in strain: SK1. 1
Natural varianti659P → S in strain: SK1. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D11463 Genomic DNA Translation: BAA02017.1
Z49939 Genomic DNA Translation: CAA90195.1
U60829 Genomic DNA Translation: AAB61454.1
BK006946 Genomic DNA Translation: DAA10123.1
PIRiS57592
RefSeqiNP_013951.1, NM_001182731.1

Genome annotation databases

EnsemblFungiiYMR224C; YMR224C; YMR224C
GeneIDi855264
KEGGisce:YMR224C

Similar proteinsi

Entry informationi

Entry nameiMRE11_YEAST
AccessioniPrimary (citable) accession number: P32829
Secondary accession number(s): D6W049, P87289, Q07173
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 184 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health