Reviewed, UniProtKB/Swiss-Prot P32824 (KLKR_PRANA)

Names and origin

Protein names	Recommended name:     Renal glandular kallikrein     EC=3.4.21.35 Alternative name(s):     Tissue kallikrein
Organism	Praomys natalensis (African soft-furred rat) (Mastomys natalensis)
Taxonomic identifier	10112 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mastomys

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Catalytic activity	Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Probable
	Propeptide	19 – 24	6	Activation peptide Probable		PRO_0000027997
	Chain	25 – 263	239	Renal glandular kallikrein		PRO_0000027998
Regions
	Domain	25 – 260	236	Peptidase S1
Sites
	Active site	65	1	Charge relay system
	Active site	121	1	Charge relay system
	Active site	215	1	Charge relay system
Amino acid modifications
	Glycosylation	102	1	N-linked (GlcNAc...) Probable
	Disulfide bond	31 ↔ 175		By similarity
	Disulfide bond	50 ↔ 66		By similarity
	Disulfide bond	153 ↔ 221		By similarity
	Disulfide bond	186 ↔ 200		By similarity
	Disulfide bond	211 ↔ 236		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P32824-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: A8EB023B800337D5	FASTA	263	29,130
	10 20 30 40 50 60 MWFLILFLAL FLGGIDAAPP VQSRIIGGFN CEKNSQPWHV AVYRFARYQC GGVLLDANWV 70 80 90 100 110 120 LTAAHCYNDK YQVWLGKNNR FEDEPSAQHQ LISKAIPHPG FNMSLLNKDH TPHPEDDYSN 130 140 150 160 170 180 DLMLVRLKKP AEITDVVKPI DLPTEEPTVG SRCLASGWGS TTPTEEFEYS HDLQCVYLEL 190 200 210 220 230 240 LSNEVCAKAH TEKVTDTMLC AGEMDGGKDT CVGDSGGPLI CDGVLQGITS WGPTPCALPN 250 260 VPGIYTKLIE YRSWIKDVMA NNP

References

[1]	"Characterization of kallikrein cDNAs from the African rodent Mastomys." Fahnestock M. DNA Cell Biol. 13:293-300(1994) [PubMed: 7909667] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Salivary gland.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X17352 mRNA. Translation: CAA35232.1.
PIR	S15686. I83227.
3D structure databases
HSSP	HSSP built from PDB template 1SGF based on UniProtKB P00757.
SMR	P32824. Positions 25-263.
ModBase	Search...
Protein family/group databases
MEROPS	S01.290.
Phylogenomic databases
HOVERGEN	P32824.
Enzyme and pathway databases
BRENDA	3.4.21.35. 273805.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KLKR_PRANA

Accession

Primary (citable) accession number: P32824

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	May 26, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)