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P32824 (KLKR_MASNA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Renal glandular kallikrein

EC=3.4.21.35
Alternative name(s):
Tissue kallikrein
OrganismMastomys natalensis (African soft-furred rat) (Praomys natalensis)
Taxonomic identifier10112 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMastomys

Protein attributes

Sequence length263 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Catalytic activity

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Sequence similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

Contains 1 peptidase S1 domain.

Ontologies

Keywords
   DomainSignal
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Glycoprotein
Zymogen
Gene Ontology (GO)
   Molecular_functionserine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Probable
Propeptide19 – 246Activation peptide Probable
PRO_0000027997
Chain25 – 263239Renal glandular kallikrein
PRO_0000027998

Regions

Domain25 – 260236Peptidase S1

Sites

Active site651Charge relay system
Active site1211Charge relay system
Active site2151Charge relay system

Amino acid modifications

Glycosylation1021N-linked (GlcNAc...) Probable
Disulfide bond31 ↔ 175 By similarity
Disulfide bond50 ↔ 66 By similarity
Disulfide bond153 ↔ 221 By similarity
Disulfide bond186 ↔ 200 By similarity
Disulfide bond211 ↔ 236 By similarity

Sequences

Sequence LengthMass (Da)Tools
P32824 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: A8EB023B800337D5

FASTA26329,130
        10         20         30         40         50         60 
MWFLILFLAL FLGGIDAAPP VQSRIIGGFN CEKNSQPWHV AVYRFARYQC GGVLLDANWV 

        70         80         90        100        110        120 
LTAAHCYNDK YQVWLGKNNR FEDEPSAQHQ LISKAIPHPG FNMSLLNKDH TPHPEDDYSN 

       130        140        150        160        170        180 
DLMLVRLKKP AEITDVVKPI DLPTEEPTVG SRCLASGWGS TTPTEEFEYS HDLQCVYLEL 

       190        200        210        220        230        240 
LSNEVCAKAH TEKVTDTMLC AGEMDGGKDT CVGDSGGPLI CDGVLQGITS WGPTPCALPN 

       250        260 
VPGIYTKLIE YRSWIKDVMA NNP 

« Hide

References

[1]"Characterization of kallikrein cDNAs from the African rodent Mastomys."
Fahnestock M.
DNA Cell Biol. 13:293-300(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Salivary gland.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X17352 mRNA. Translation: CAA35232.1.
PIRS15686. I83227.

3D structure databases

ProteinModelPortalP32824.
SMRP32824. Positions 25-263.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS01.290.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG013304.

Enzyme and pathway databases

BRENDA3.4.21.35. 5009.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKLKR_MASNA
AccessionPrimary (citable) accession number: P32824
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 14, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries