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Protein

Renal glandular kallikrein

Gene
N/A
Organism
Mastomys natalensis (African soft-furred rat) (Praomys natalensis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Catalytic activityi

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Charge relay system
Active sitei121 – 1211Charge relay system
Active sitei215 – 2151Charge relay system

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.35. 5009.

Protein family/group databases

MEROPSiS01.290.

Names & Taxonomyi

Protein namesi
Recommended name:
Renal glandular kallikrein (EC:3.4.21.35)
Alternative name(s):
Tissue kallikrein
OrganismiMastomys natalensis (African soft-furred rat) (Praomys natalensis)
Taxonomic identifieri10112 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMastomys

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818CuratedAdd
BLAST
Propeptidei19 – 246Activation peptideCuratedPRO_0000027997
Chaini25 – 263239Renal glandular kallikreinPRO_0000027998Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 175PROSITE-ProRule annotation
Disulfide bondi50 ↔ 66PROSITE-ProRule annotation
Glycosylationi102 – 1021N-linked (GlcNAc...)Curated
Disulfide bondi153 ↔ 221PROSITE-ProRule annotation
Disulfide bondi186 ↔ 200PROSITE-ProRule annotation
Disulfide bondi211 ↔ 236PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Structurei

3D structure databases

ProteinModelPortaliP32824.
SMRiP32824. Positions 25-263.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 260236Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG013304.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32824-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MWFLILFLAL FLGGIDAAPP VQSRIIGGFN CEKNSQPWHV AVYRFARYQC
60 70 80 90 100
GGVLLDANWV LTAAHCYNDK YQVWLGKNNR FEDEPSAQHQ LISKAIPHPG
110 120 130 140 150
FNMSLLNKDH TPHPEDDYSN DLMLVRLKKP AEITDVVKPI DLPTEEPTVG
160 170 180 190 200
SRCLASGWGS TTPTEEFEYS HDLQCVYLEL LSNEVCAKAH TEKVTDTMLC
210 220 230 240 250
AGEMDGGKDT CVGDSGGPLI CDGVLQGITS WGPTPCALPN VPGIYTKLIE
260
YRSWIKDVMA NNP
Length:263
Mass (Da):29,130
Last modified:October 1, 1993 - v1
Checksum:iA8EB023B800337D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17352 mRNA. Translation: CAA35232.1.
PIRiI83227. S15686.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17352 mRNA. Translation: CAA35232.1.
PIRiI83227. S15686.

3D structure databases

ProteinModelPortaliP32824.
SMRiP32824. Positions 25-263.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS01.290.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG013304.

Enzyme and pathway databases

BRENDAi3.4.21.35. 5009.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization of kallikrein cDNAs from the African rodent Mastomys."
    Fahnestock M.
    DNA Cell Biol. 13:293-300(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Salivary gland.

Entry informationi

Entry nameiKLKR_MASNA
AccessioniPrimary (citable) accession number: P32824
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: October 1, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.