Reviewed, UniProtKB/Swiss-Prot P32823 (CHIA_PSEO7)

Names and origin

Protein names

Recommended name:
    Chitinase A
      Short name=CHI-A
    EC=3.2.1.14

Gene names

Name:

chiA

Organism

Pseudoalteromonas piscicida

Taxonomic identifier

43662 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Pseudoalteromonadaceae › Pseudoalteromonas

Protein attributes

Sequence length	820 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Enzyme regulation	Stimulated by magnesium ions; inhibited by N-bromosuccinimide and 2-hydroxy-5-nitrobenzyl bromide.
Sequence similarities	Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.
biophysicochemical properties	pH dependence: Optimum pH is 8.0. Temperature dependence: Optimum temperature is 50 degrees Celsius.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: InterPro
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro cation binding Inferred from electronic annotation. Source: InterPro chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Ref.2
	Chain	22 – 820	799	Chitinase A		PRO_0000011904
Sites
	Active site	313	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P32823-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: FFD70D91192BA320	FASTA	820	87,346
	10 20 30 40 50 60 MKLNKITSYI GFALLSGGAL AAPSTPTLDW QPQQYSFVEV NVDGLGSYKQ LVKAKDVVDI 70 80 90 100 110 120 SIKWNAWSGS GGDNYKVYFD DLLVNQGSLP AGTKSGVVQF PYTKSGRHQL YLELCEGTVC 130 140 150 160 170 180 ARSAGKEIVI ADTDGAHLAP LPMNVDPNNR NNGTIPGRVT GAYFVEWGIY GRNYDVTKIP 190 200 210 220 230 240 AHNLSHILYG FIPICGPNES LKSIEIGNSW RALQTACADS QDYEVVIHDP WAAVQKSMPG 250 260 270 280 290 300 VDAKDPIRGV YSQLMALKQR YPDLKILPSV GGWTLSDPFH GFTNKANRDT FVASVKQFLK 310 320 330 340 350 360 TWKFYDGVDI DWEFPGGDGP NPDLGDPIND GPAYVALMQE LRAMLDELEA ETGRQYELTS 370 380 390 400 410 420 AIGAGYDKIE DVDYQAAQQY MDYIFAMTYD FYGAWNNETG HQTGIYCGSH LSTDECNGTG 430 440 450 460 470 480 VDDNGVPRKG PAYTGDHAIQ LLLQQGVQPS KLVMGVAMYG RGWEGVLDAN AAIPGNPMTA 490 500 510 520 530 540 PGNGPLTGST SEGVWEPGIM DYKAIAANAV GQGGSGVNGY EVGYDEQAQA AYVWNRSNGK 550 560 570 580 590 600 LITYDSPRSV IAKGQYANTH QLAGLFGWEI DADNGDILNA MYDGLTAGEI PNRAPTIGVS 610 620 630 640 650 660 GPINVTSGQV VNVDAQASDL DNDPLTYSWV AAPGLALSAN NTAAVAVTAP SVAQQTSYDL 670 680 690 700 710 720 TVTVNDGALS TTKTIVVVVN PEGANAAPVV TPVSDISVNE GASATVNVSA TDPEGAALSY 730 740 750 760 770 780 SWSVPAELSV ANGSSATITA ANVTADTTVP VTVTVSDGVN AVDTTFNVTI KDGAEYPTWD 790 800 810 820 RSTVYVGGDR VIHNSNVFEA KWWTQGEEPG TADVWKAVTN

References

[1]	"Cloning, sequence, and expression of a chitinase gene from a marine bacterium, Altermonas sp. strain O-7." Tsujibo H., Orikoshi H., Tanno H., Fujimoto K., Miyamoto K., Imada C., Okami Y., Inamori Y. J. Bacteriol. 175:176-181(1993) [PubMed: 8416892] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: O-7.
[2]	"Purification, properties, and partial amino acid sequence of chitinase from a marine Alteromonas sp. strain O-7." Tsujibo H., Yoshida Y., Miyamoto K., Imada C., Okami Y., Inamori Y. Can. J. Microbiol. 38:891-897(1992) [PubMed: 1464065] [Abstract] Cited for: PROTEIN SEQUENCE OF 22-48, CHARACTERIZATION. Strain: O-7.
[3]	"Site-directed mutagenesis of chitinase from Alteromonas sp. strain O-7." Tsujibo H., Orikoshi H., Imada C., Okami Y., Miyamoto K., Inamori Y. Biosci. Biotechnol. Biochem. 57:1396-1397(1993) [PubMed: 7764022] [Abstract] Cited for: MUTAGENESIS. Strain: O-7.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB063629 Genomic DNA. Translation: BAB79620.1.
PIR	A40633. PC4106.
3D structure databases
HSSP	HSSP built from PDB template 1EDQ based on UniProtKB O83008.
ModBase	Search...
Protein family/group databases
CAZy	CBM5. Carbohydrate-Binding Module Family 5. GH18. Glycoside Hydrolase Family 18.
Family and domain databases
InterPro	IPR003610. CBM_5_12. IPR011583. Chitinase_II. IPR013540. ChitinaseA_N. IPR001223. Glyco_hydro18cat. IPR001579. Glyco_hydro_18_chit_AS. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. IPR000601. PKD. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF02839. CBM_5_12. 1 hit. PF08329. ChitinaseA_N. 1 hit. PF00704. Glyco_hydro_18. 1 hit. PF00801. PKD. 2 hits. [Graphical view]
ProDom	PD000471. Chitinase_II. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00495. ChtBD3. 1 hit. SM00636. Glyco_18. 1 hit. SM00089. PKD. 2 hits. [Graphical view]
PROSITE	PS01095. CHITINASE_18. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHIA_PSEO7

Accession

Primary (citable) accession number: P32823

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	June 16, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)