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P32822 (TRYB_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trypsin V-B

EC=3.4.21.4
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length246 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Cofactor

Binds 1 calcium ion per subunit By similarity.

Subcellular location

Secretedextracellular space.

Sequence similarities

Belongs to the peptidase S1 family.

Contains 1 peptidase S1 domain.

Ontologies

Keywords
   Biological processDigestion
   Cellular componentSecreted
   DomainSignal
   LigandCalcium
Metal-binding
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Zymogen
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdigestion

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

serine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1515
Propeptide16 – 249Activation peptide
PRO_0000028217
Chain25 – 246222Trypsin V-B
PRO_0000028218

Regions

Domain25 – 244220Peptidase S1

Sites

Active site641Charge relay system By similarity
Active site1081Charge relay system By similarity
Active site2001Charge relay system By similarity
Metal binding761Calcium By similarity
Metal binding781Calcium; via carbonyl oxygen By similarity
Metal binding861Calcium By similarity
Site1941Required for specificity By similarity

Amino acid modifications

Disulfide bond31 ↔ 160 By similarity
Disulfide bond49 ↔ 65 By similarity
Disulfide bond133 ↔ 233 By similarity
Disulfide bond140 ↔ 206 By similarity
Disulfide bond171 ↔ 185 By similarity
Disulfide bond196 ↔ 220 By similarity

Sequences

Sequence LengthMass (Da)Tools
P32822 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 1EB899CA1BAB0025

FASTA24626,820
        10         20         30         40         50         60 
MKICIFFTLL GTVAAFPTED NDDRIVGGYT CQEHSVPYQV SLNAGSHICG GSLITDQWVL 

        70         80         90        100        110        120 
SAAHCYHPQL QVRLGEHNIY EIEGAEQFID AAKMILHPDY DKWTVDNDIM LIKLKSPATL 

       130        140        150        160        170        180 
NSKVSTIPLP QYCPTAGTEC LVSGWGVLKF GFESPSVLQC LDAPVLSDSV CHKAYPRQIT 

       190        200        210        220        230        240 
NNMFCLGFLE GGKDSCQYDS GGPVVCNGEV QGIVSWGDGC ALEGKPGVYT KVCNYLNWIQ 


QTVAAN 

« Hide

References

[1]"Identification of cDNAs encoding two novel rat pancreatic serine proteases."
Kang J., Wiegand U., Mueller-Hill B.
Gene 110:181-187(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Pancreas.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X59013 mRNA. Translation: CAA41752.1.
PIRJQ1472.
UniGeneRn.13006.

3D structure databases

ProteinModelPortalP32822.
SMRP32822. Positions 25-246.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS01.093.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG013304.
InParanoidP32822.
PhylomeDBP32822.

Gene expression databases

GenevestigatorP32822.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRYB_RAT
AccessionPrimary (citable) accession number: P32822
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 14, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries