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Protein

Trypsin V-B

Gene
N/A
Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei64 – 641Charge relay systemBy similarity
Metal bindingi76 – 761CalciumBy similarity
Metal bindingi78 – 781Calcium; via carbonyl oxygenBy similarity
Metal bindingi86 – 861CalciumBy similarity
Active sitei108 – 1081Charge relay systemBy similarity
Sitei194 – 1941Required for specificityBy similarity
Active sitei200 – 2001Charge relay systemBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. digestion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Digestion

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

MEROPSiS01.093.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin V-B (EC:3.4.21.4)
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1515Add
BLAST
Propeptidei16 – 249Activation peptidePRO_0000028217
Chaini25 – 246222Trypsin V-BPRO_0000028218Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 160PROSITE-ProRule annotation
Disulfide bondi49 ↔ 65PROSITE-ProRule annotation
Disulfide bondi133 ↔ 233PROSITE-ProRule annotation
Disulfide bondi140 ↔ 206PROSITE-ProRule annotation
Disulfide bondi171 ↔ 185PROSITE-ProRule annotation
Disulfide bondi196 ↔ 220PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Expressioni

Gene expression databases

GenevestigatoriP32822.

Structurei

3D structure databases

ProteinModelPortaliP32822.
SMRiP32822. Positions 25-246.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 244220Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG013304.
PhylomeDBiP32822.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32822-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKICIFFTLL GTVAAFPTED NDDRIVGGYT CQEHSVPYQV SLNAGSHICG
60 70 80 90 100
GSLITDQWVL SAAHCYHPQL QVRLGEHNIY EIEGAEQFID AAKMILHPDY
110 120 130 140 150
DKWTVDNDIM LIKLKSPATL NSKVSTIPLP QYCPTAGTEC LVSGWGVLKF
160 170 180 190 200
GFESPSVLQC LDAPVLSDSV CHKAYPRQIT NNMFCLGFLE GGKDSCQYDS
210 220 230 240
GGPVVCNGEV QGIVSWGDGC ALEGKPGVYT KVCNYLNWIQ QTVAAN
Length:246
Mass (Da):26,820
Last modified:September 30, 1993 - v1
Checksum:i1EB899CA1BAB0025
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59013 mRNA. Translation: CAA41752.1.
PIRiJQ1472.
UniGeneiRn.13006.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59013 mRNA. Translation: CAA41752.1.
PIRiJQ1472.
UniGeneiRn.13006.

3D structure databases

ProteinModelPortaliP32822.
SMRiP32822. Positions 25-246.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS01.093.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG013304.
PhylomeDBiP32822.

Gene expression databases

GenevestigatoriP32822.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification of cDNAs encoding two novel rat pancreatic serine proteases."
    Kang J., Wiegand U., Mueller-Hill B.
    Gene 110:181-187(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pancreas.

Entry informationi

Entry nameiTRYB_RAT
AccessioniPrimary (citable) accession number: P32822
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 30, 1993
Last sequence update: September 30, 1993
Last modified: January 6, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.