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P32822

- TRYB_RAT

UniProt

P32822 - TRYB_RAT

Protein

Trypsin V-B

Gene
N/A
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

    Cofactori

    Binds 1 calcium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei64 – 641Charge relay systemBy similarity
    Metal bindingi76 – 761CalciumBy similarity
    Metal bindingi78 – 781Calcium; via carbonyl oxygenBy similarity
    Metal bindingi86 – 861CalciumBy similarity
    Active sitei108 – 1081Charge relay systemBy similarity
    Sitei194 – 1941Required for specificityBy similarity
    Active sitei200 – 2001Charge relay systemBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. digestion Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Digestion

    Keywords - Ligandi

    Calcium, Metal-binding

    Protein family/group databases

    MEROPSiS01.093.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trypsin V-B (EC:3.4.21.4)
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1515Add
    BLAST
    Propeptidei16 – 249Activation peptidePRO_0000028217
    Chaini25 – 246222Trypsin V-BPRO_0000028218Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi31 ↔ 160PROSITE-ProRule annotation
    Disulfide bondi49 ↔ 65PROSITE-ProRule annotation
    Disulfide bondi133 ↔ 233PROSITE-ProRule annotation
    Disulfide bondi140 ↔ 206PROSITE-ProRule annotation
    Disulfide bondi171 ↔ 185PROSITE-ProRule annotation
    Disulfide bondi196 ↔ 220PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Zymogen

    Expressioni

    Gene expression databases

    GenevestigatoriP32822.

    Structurei

    3D structure databases

    ProteinModelPortaliP32822.
    SMRiP32822. Positions 25-246.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 244220Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOVERGENiHBG013304.
    InParanoidiP32822.
    PhylomeDBiP32822.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P32822-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKICIFFTLL GTVAAFPTED NDDRIVGGYT CQEHSVPYQV SLNAGSHICG    50
    GSLITDQWVL SAAHCYHPQL QVRLGEHNIY EIEGAEQFID AAKMILHPDY 100
    DKWTVDNDIM LIKLKSPATL NSKVSTIPLP QYCPTAGTEC LVSGWGVLKF 150
    GFESPSVLQC LDAPVLSDSV CHKAYPRQIT NNMFCLGFLE GGKDSCQYDS 200
    GGPVVCNGEV QGIVSWGDGC ALEGKPGVYT KVCNYLNWIQ QTVAAN 246
    Length:246
    Mass (Da):26,820
    Last modified:October 1, 1993 - v1
    Checksum:i1EB899CA1BAB0025
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59013 mRNA. Translation: CAA41752.1.
    PIRiJQ1472.
    UniGeneiRn.13006.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59013 mRNA. Translation: CAA41752.1 .
    PIRi JQ1472.
    UniGenei Rn.13006.

    3D structure databases

    ProteinModelPortali P32822.
    SMRi P32822. Positions 25-246.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S01.093.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG013304.
    InParanoidi P32822.
    PhylomeDBi P32822.

    Gene expression databases

    Genevestigatori P32822.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of cDNAs encoding two novel rat pancreatic serine proteases."
      Kang J., Wiegand U., Mueller-Hill B.
      Gene 110:181-187(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Pancreas.

    Entry informationi

    Entry nameiTRYB_RAT
    AccessioniPrimary (citable) accession number: P32822
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3