Reviewed, UniProtKB/Swiss-Prot P32822 (TRYB_RAT)

Names and origin

Protein names	Recommended name:     Trypsin V-B     EC=3.4.21.4
Organism	Rattus norvegicus (Rat)
Taxonomic identifier	10116 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15
	Propeptide	16 – 24	9	Activation peptide		PRO_0000028217
	Chain	25 – 246	222	Trypsin V-B		PRO_0000028218
Regions
	Domain	25 – 244	220	Peptidase S1
Sites
	Active site	64	1	Charge relay system By similarity
	Active site	108	1	Charge relay system By similarity
	Active site	200	1	Charge relay system By similarity
	Metal binding	76	1	Calcium By similarity
	Metal binding	78	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	86	1	Calcium By similarity
	Site	194	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	31 ↔ 160		By similarity
	Disulfide bond	49 ↔ 65		By similarity
	Disulfide bond	133 ↔ 233		By similarity
	Disulfide bond	140 ↔ 206		By similarity
	Disulfide bond	171 ↔ 185		By similarity
	Disulfide bond	196 ↔ 220		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P32822-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 1EB899CA1BAB0025	FASTA	246	26,820
	10 20 30 40 50 60 MKICIFFTLL GTVAAFPTED NDDRIVGGYT CQEHSVPYQV SLNAGSHICG GSLITDQWVL 70 80 90 100 110 120 SAAHCYHPQL QVRLGEHNIY EIEGAEQFID AAKMILHPDY DKWTVDNDIM LIKLKSPATL 130 140 150 160 170 180 NSKVSTIPLP QYCPTAGTEC LVSGWGVLKF GFESPSVLQC LDAPVLSDSV CHKAYPRQIT 190 200 210 220 230 240 NNMFCLGFLE GGKDSCQYDS GGPVVCNGEV QGIVSWGDGC ALEGKPGVYT KVCNYLNWIQ QTVAAN

References

[1]	"Identification of cDNAs encoding two novel rat pancreatic serine proteases." Kang J., Wiegand U., Mueller-Hill B. Gene 110:181-187(1992) [PubMed: 1537555] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pancreas.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X59013 mRNA. Translation: CAA41752.1.
IPI	IPI00191680.
PIR	JQ1472.
3D structure databases
HSSP	HSSP built from PDB template 1EZX based on UniProtKB P00760.
SMR	P32822. Positions 25-246.
ModBase	Search...
Protein family/group databases
MEROPS	S01.093.
Genome annotation databases
Ensembl	ENSRNOG00000013203. Rattus norvegicus. [Contig view]
Phylogenomic databases
HOVERGEN	P32822.
Enzyme and pathway databases
BRENDA	3.4.21.4. 248.
Gene expression databases
ArrayExpress	P32822.
GermOnline	ENSRNOG00000013203. Rattus norvegicus.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRYB_RAT

Accession

Primary (citable) accession number: P32822

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	June 16, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)