P32821 (TRYA_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Trypsin V-A EC=3.4.21.4
Organism	Rattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier	10116 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15
	Propeptide	16 – 24	9	Activation peptide		PRO_0000028215
	Chain	25 – 246	222	Trypsin V-A		PRO_0000028216
Regions
	Domain	25 – 244	220	Peptidase S1
Sites
	Active site	64	1	Charge relay system By similarity
	Active site	108	1	Charge relay system By similarity
	Active site	200	1	Charge relay system By similarity
	Metal binding	76	1	Calcium By similarity
	Metal binding	78	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	86	1	Calcium By similarity
	Site	194	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	31 ↔ 160		By similarity
	Disulfide bond	49 ↔ 65		By similarity
	Disulfide bond	133 ↔ 233		By similarity
	Disulfide bond	140 ↔ 206		By similarity
	Disulfide bond	171 ↔ 185		By similarity
	Disulfide bond	196 ↔ 220		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P32821 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 1EBE59D88BAB1715	FASTA	246	26,901
	10 20 30 40 50 60 MKICIFFTLL GTVAAFPTED NDDRIVGGYT CQEHSVPYQV SLNAGSHICG GSLITDQWVL 70 80 90 100 110 120 SAAHCYHPQL QVRLGEHNIY EIEGAEQFID AAKMILHPDY DKWTVDNDIM LIKLKSPATL 130 140 150 160 170 180 NSKVSTIPLP QYCPTAGTEC LVSGWGVLKF GFESPSVLQC LDAPVLSDSV CHKAYPRQIT 190 200 210 220 230 240 NNMFCLGFLE GGKDSCQYDS GGPVVCNGEV QGIVSWGDGC ALEGKPGVYT KVCNYLNWIH QTIAEN

References

[1]	"Identification of cDNAs encoding two novel rat pancreatic serine proteases." Kang J., Wiegand U., Mueller-Hill B. Gene 110:181-187(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pancreas.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X59012 mRNA. Translation: CAA41751.1.
IPI	IPI00191677.
PIR	JQ1471.
RefSeq	NP_001101326.1. NM_001107856.1.
UniGene	Rn.13006.
3D structure databases
ProteinModelPortal	P32821.
SMR	P32821. Positions 25-246.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000017673.
Protein family/group databases
MEROPS	S01.092.
Proteomic databases
PaxDb	P32821.
PRIDE	P32821.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000017673; ENSRNOP00000017673; ENSRNOG00000013203.
GeneID	312273.
KEGG	rno:312273.
UCSC	RGD:1563848. rat.
Organism-specific databases
RGD	1563848. LOC312273.
Phylogenomic databases
eggNOG	COG5640.
GeneTree	ENSGT00680000099557.
HOGENOM	HOG000251820.
HOVERGEN	HBG013304.
InParanoid	P32821.
OMA	NAGSHIC.
OrthoDB	EOG4SBF0W.
Gene expression databases
ArrayExpress	P32821.
Genevestigator	P32821.
GermOnline	ENSRNOG00000013203. Rattus norvegicus.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	664738.

Entry information

Entry name

TRYA_RAT

Accession

Primary (citable) accession number: P32821

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	April 3, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program