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P32821

- TRYA_RAT

UniProt

P32821 - TRYA_RAT

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Protein
Trypsin V-A
Gene
N/A
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Cofactori

Binds 1 calcium ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei64 – 641Charge relay system By similarity
Metal bindingi76 – 761Calcium By similarity
Metal bindingi78 – 781Calcium; via carbonyl oxygen By similarity
Metal bindingi86 – 861Calcium By similarity
Active sitei108 – 1081Charge relay system By similarity
Sitei194 – 1941Required for specificity By similarity
Active sitei200 – 2001Charge relay system By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. digestion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Digestion

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

MEROPSiS01.092.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin V-A (EC:3.4.21.4)
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 4

Organism-specific databases

RGDi1563848. LOC312273.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1515
Add
BLAST
Propeptidei16 – 249Activation peptide
PRO_0000028215
Chaini25 – 246222Trypsin V-A
PRO_0000028216Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 160 By similarity
Disulfide bondi49 ↔ 65 By similarity
Disulfide bondi133 ↔ 233 By similarity
Disulfide bondi140 ↔ 206 By similarity
Disulfide bondi171 ↔ 185 By similarity
Disulfide bondi196 ↔ 220 By similarity

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP32821.
PRIDEiP32821.

Expressioni

Gene expression databases

GenevestigatoriP32821.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017673.

Structurei

3D structure databases

ProteinModelPortaliP32821.
SMRiP32821. Positions 25-246.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 244220Peptidase S1
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00740000115192.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP32821.
OMAiAGSHICG.
OrthoDBiEOG75B84T.
TreeFamiTF331065.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32821-1 [UniParc]FASTAAdd to Basket

« Hide

MKICIFFTLL GTVAAFPTED NDDRIVGGYT CQEHSVPYQV SLNAGSHICG    50
GSLITDQWVL SAAHCYHPQL QVRLGEHNIY EIEGAEQFID AAKMILHPDY 100
DKWTVDNDIM LIKLKSPATL NSKVSTIPLP QYCPTAGTEC LVSGWGVLKF 150
GFESPSVLQC LDAPVLSDSV CHKAYPRQIT NNMFCLGFLE GGKDSCQYDS 200
GGPVVCNGEV QGIVSWGDGC ALEGKPGVYT KVCNYLNWIH QTIAEN 246
Length:246
Mass (Da):26,901
Last modified:October 1, 1993 - v1
Checksum:i1EBE59D88BAB1715
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59012 mRNA. Translation: CAA41751.1.
PIRiJQ1471.
RefSeqiNP_001101326.1. NM_001107856.1.
UniGeneiRn.13006.

Genome annotation databases

EnsembliENSRNOT00000017673; ENSRNOP00000017673; ENSRNOG00000013203.
GeneIDi312273.
KEGGirno:312273.
UCSCiRGD:1563848. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59012 mRNA. Translation: CAA41751.1 .
PIRi JQ1471.
RefSeqi NP_001101326.1. NM_001107856.1.
UniGenei Rn.13006.

3D structure databases

ProteinModelPortali P32821.
SMRi P32821. Positions 25-246.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000017673.

Protein family/group databases

MEROPSi S01.092.

Proteomic databases

PaxDbi P32821.
PRIDEi P32821.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000017673 ; ENSRNOP00000017673 ; ENSRNOG00000013203 .
GeneIDi 312273.
KEGGi rno:312273.
UCSCi RGD:1563848. rat.

Organism-specific databases

RGDi 1563848. LOC312273.

Phylogenomic databases

eggNOGi COG5640.
GeneTreei ENSGT00740000115192.
HOGENOMi HOG000251820.
HOVERGENi HBG013304.
InParanoidi P32821.
OMAi AGSHICG.
OrthoDBi EOG75B84T.
TreeFami TF331065.

Miscellaneous databases

NextBioi 664738.

Gene expression databases

Genevestigatori P32821.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Identification of cDNAs encoding two novel rat pancreatic serine proteases."
    Kang J., Wiegand U., Mueller-Hill B.
    Gene 110:181-187(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pancreas.

Entry informationi

Entry nameiTRYA_RAT
AccessioniPrimary (citable) accession number: P32821
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 14, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi