Reviewed, UniProtKB/Swiss-Prot P32818 (AMYM_BACAD)

Names and origin

Protein names	Recommended name:     Maltogenic alpha-amylase     EC=3.2.1.133 Alternative name(s):     Glucan 1,4-alpha-maltohydrolase
Organism	Bacillus acidopullulyticus
Taxonomic identifier	28030 [NCBI]
Taxonomic lineage	Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	586 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts starch into maltose.
Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive alpha-maltose residues from the non-reducing ends of the chains.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Ligand	Calcium Metal-binding
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW glucan 1,4-alpha-maltohydrolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 586	586	Maltogenic alpha-amylase		PRO_0000054295
Sites
	Active site	328	1	Nucleophile By similarity
	Active site	357	1	Proton donor By similarity
	Active site	424	1	By similarity
	Metal binding	147	1	Calcium By similarity
	Metal binding	152	1	Calcium By similarity
	Metal binding	153	1	Calcium By similarity
	Metal binding	172	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	174	1	Calcium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P32818-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: EEC050711DCD627A	FASTA	586	68,520
	10 20 30 40 50 60 MFREAIYHRP KDNYAYACAN GELHIRIRTK KDDMKEVTLV YGDTYEFDDN KWTYSTAHMK 70 80 90 100 110 120 KTGSDQLFDY WLASVTPRYK RLRYGFIVND RKDSTCFTEK GFYPEPPVND ISYYFSFPYV 130 140 150 160 170 180 NQADIFQAPE WVKDTVWYQI FPERFANGNK DNDPDGTLPW GSREPEIDNF FGGDLEGVIE 190 200 210 220 230 240 HIDYLKELGI GGIYFTPIFK AHSNHKYDTI DYMEIDPQFG TKETLKKLID VCHKNGIKVM 250 260 270 280 290 300 LDAVFNHSGV FFPPFQDVVE KGKNSKYQDW FHIREFPLVM EPRPNYDTFG FTSSMPKFKT 310 320 330 340 350 360 ENPEVKEYLL EVGRYWVREF DIDGWRLDVA NEVSHAFWRE FRREVKAIKP DLYILGEIWH 370 380 390 400 410 420 DSLPWLRGDQ FDAVMNYPLS TNIVNLFANQ SVSVKEFVEN MSHVIHMYPD TVNEAAFNLV 430 440 450 460 470 480 GSHDTPRILT QCGEDVERLK QVFVLLLTFI GTPCIYYGDE IGLTGGQDPG CRKCMEWNPD 490 500 510 520 530 540 QQNRELLQHV RKLIQLRSEN PLLANEGELT FVPPKNEEDP CLAYTKSNEE KTIMIIINKS 550 560 570 580 KNSVEYPLSF DAAEQTVKNL WNQEQLILQN GQTLELKAND FKILEF

References

[1]	"Molecular genetics analysis of a maltogenic amylase gene of Bacillus acidopullulyticus." Kelly A.P., Diderichsen B., Jorgensen S.T., McConnell D.J. Submitted (APR-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z22520 Genomic DNA. Translation: CAA80246.1.
PIR	S34731.
3D structure databases
HSSP	HSSP built from PDB template 1J0H based on UniProtKB P38940.
ModBase	Search...
Protein family/group databases
CAZy	CBM34. Carbohydrate-Binding Module Family 34. GH13. Glycoside Hydrolase Family 13.
Enzyme and pathway databases
BRENDA	3.2.1.133. 97501.
Family and domain databases
InterPro	IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR004185. Glyco_hydro_13_lg-like. IPR006589. Glyco_hydro_13_sub_cat. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02903. Alpha-amylase_N. 1 hit. [Graphical view]
SMART	SM00642. Aamy. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMYM_BACAD

Accession

Primary (citable) accession number: P32818

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)