Reviewed, UniProtKB/Swiss-Prot P32811 (PHSH_SOLTU)

Names and origin

Protein names	Recommended name:     Alpha-glucan phosphorylase, H isozyme     EC=2.4.1.1 Alternative name(s):     Starch phosphorylase H
Organism	Solanum tuberosum (Potato)
Taxonomic identifier	4113 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	838 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.
Catalytic activity	(1,4-alpha-D-glucosyl)(n) + phosphate = (1,4-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate.
Cofactor	Pyridoxal phosphate.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the glycogen phosphorylase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Glycosyltransferase Transferase
Technical term	Allosteric enzyme Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphorylase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 838	838	Alpha-glucan phosphorylase, H isozyme		PRO_0000188542
Amino acid modifications
	Modified residue	684	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P32811-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 1F3A55BE0F0167B4	FASTA	838	95,112
	10 20 30 40 50 60 MEGGAKSNDV SAAPIAQPLS EDPTDIASNI KYHAQYTPHF SPFKFEPLQA YYAATADSVR 70 80 90 100 110 120 DRLIKQWNDT YLHYDKVNPK QTYYLSMEYL QGRALTNAVG NLDIHNAYAD ALNKLGQQLE 130 140 150 160 170 180 EVVEQEKDAA LGNGGLGRLA SCFLDSMATL NLPAWGYGLR YRYGLFKQLI TKAGQEEVPE 190 200 210 220 230 240 DWLEKFSPWE IVRHDVVFPI RFFGHVEVLP SGSRKWVGGE VLQALAYDVP IPGYRTKNTN 250 260 270 280 290 300 SLRLWEAKAS SEDFNLFLFN DGQYDAAAQL HSRAQQICAV LYPGDATENG KLLRLKQQFF 310 320 330 340 350 360 LCSASLQDII ARFKEREDGK GSHQWSEFPK KVAIQLNDTH PTLTIPELMR LLMDDEGLGW 370 380 390 400 410 420 DESWNITTRT IAYTNHTVLP EALEKWSQAV MWKLLPRHME IIEEIDKRFV ATIMSERPDL 430 440 450 460 470 480 ENKMPSMRIL DHNATKPVVH MANLCVVSSH TVNGVAQLHS DILKAELFAD YVSVWPTKFQ 490 500 510 520 530 540 NKTNGITPRR WIRFCSPELS HIITKWLKTD QWVTNLELLA NLREFADNSE LHAEWESAKM 550 560 570 580 590 600 ANKQRLAQYI LHVTGVSIDP NSLFDIQVKR IHEYKRQLLN ILGVIYRYKK LKGMSPEERK 610 620 630 640 650 660 NTTPRTVMIG GKAFATYTNA KRIVKLVTDV GDVVNSDPDV NDYLKVVFVP NYNVSVAEML 670 680 690 700 710 720 IPGSELSQHI STAGMEASGT SNMKFALNGC LIIGTLDGAN VEIREEIGED NFFLFGATAD 730 740 750 760 770 780 EVPQLRKDRE NGLFKPDPRF EEAKQFIRSG AFGTYDYNPL LESLEGNSGY GRGDYFLVGH 790 800 810 820 830 DFPSYMDAQA RVDEAYKDRK RWIKMSILST SGSGKFSSDR TISQYAKEIW NIAECRVP

References

[1]

"Potato tuber type H phosphorylase isozyme. Molecular cloning, nucleotide sequence, and expression of a full-length cDNA in Escherichia coli." Mori H., Tanizawa K., Fukui T. J. Biol. Chem. 266:18446-18453(1991) [PubMed: 1917968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M69038 mRNA. Translation: AAA33809.1.
PIR	A40995.
3D structure databases
HSSP	HSSP built from PDB template 1YGP based on UniProtKB P06738.
ModBase	Search...
Protein family/group databases
CAZy	GT35. Glycosyltransferase Family 35.
Enzyme and pathway databases
BRENDA	2.4.1.1. 296.
Family and domain databases
InterPro	IPR011833. Glycg_phsphrylas. IPR000811. Glyco_trans_35. [Graphical view]
PANTHER	PTHR11468. Glyco_trans_35. 1 hit.
Pfam	PF00343. Phosphorylase. 1 hit. [Graphical view]
PIRSF	PIRSF000460. Pprylas_GlgP. 1 hit.
TIGRFAMs	TIGR02093. P_ylase. 1 hit.
PROSITE	PS00102. PHOSPHORYLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHSH_SOLTU

Accession

Primary (citable) accession number: P32811

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)