Reviewed, UniProtKB/Swiss-Prot P32810 (G3P3_CAEBR)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase 3
      Short name=GAPDH-3
    EC=1.2.1.12

Gene names

Name:	gpd-3
Synonyms:	gpd-3.1
ORF Names:	CBG14136

Organism

Caenorhabditis briggsae [Complete proteome]

Taxonomic identifier

6238 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Glyceraldehyde-3-phosphate dehydrogenase 3		PRO_0000145509
Regions
	Nucleotide binding	13 – 14	2	NAD By similarity
	Region	157 – 159	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	217 – 218	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	158	1	Nucleophile By similarity
	Binding site	35	1	NAD By similarity
	Binding site	85	1	NAD; via carbonyl oxygen By similarity
	Binding site	188	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	240	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	322	1	NAD By similarity
	Site	185	1	Activates thiol group during catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P32810-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 10EC13A5BB5A662A	FASTA	341	36,453
	10 20 30 40 50 60 MSKPSVGING FGRIGRLVLR AAVEKDSVNV VAVNDPFISI DYMVYLFQYD STHGRFKGTV 70 80 90 100 110 120 AHEGDYLLVA KEGKSQHKIK VYNSRDPAEI QWGSAGADYV VESTGVFTTI EKANAHLKGG 130 140 150 160 170 180 AKKVIISAPS ADAPMFVVGV NHEKYDHAND HIISNASCTT NCLAPLAKVI NDNFGIIEGL 190 200 210 220 230 240 MTTVHAVTAT QKTVDGPSGK LWRDGRGAGQ NIIPASTGAA KAVGKVIPEL NGKLTGMAFR 250 260 270 280 290 300 VPTPDVSVVD LTARLEKPAS LDDIKKVVKA AAEGPLKGVL AYTEDQVVST DFVSDTHSSI 310 320 330 340 FDAGASIILN PNFVKLISWY DNEFGYSNRV VDLISYIATK A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Conservation of gene organization and trans-splicing in the glyceraldehyde-3-phosphate dehydrogenase-encoding genes of Caenorhabditis briggsae." Lee Y.H., Huang X.Y., Hirsh D., Fox G.E., Hecht R.M. Gene 121:227-235(1992) [PubMed: 1446820] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of Caenorhabditis briggsae: a platform for comparative genomics." Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N., Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P., Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W., Hillier L.W. , Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A., Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E., Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K., Durbin R.M., Waterston R.H. PLoS Biol. 1:166-192(2003) [PubMed: 14624247] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AF16.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M86669 Genomic DNA. No translation available. CAAC02000521 Genomic DNA. Translation: CAP32765.1.
PIR	JH0770.
3D structure databases
HSSP	HSSP built from PDB template 3GPD based on UniProtKB P00354.
SMR	P32810. Positions 3-340.
ModBase	Search...
Organism-specific databases
WormBase	WBGene00000333. Cbr-gpd-3.1.
Phylogenomic databases
OMA	P32810. FDAKASI.
Enzyme and pathway databases
BRENDA	1.2.1.12. 261794.
Family and domain databases
InterPro	IPR000173. GlycerAld_3-P_DH. IPR006424. Glyceraldehyde-3-P_DH_1. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P3_CAEBR

Accession

Primary (citable) accession number: P32810
Secondary accession number(s): A8XJE0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	June 16, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project