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Protein

ATP-dependent DNA helicase II subunit 1

Gene

YKU70

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Single-stranded DNA-dependent ATP-dependent helicase. Involved in non-homologous end joining (NHEJ) DNA double strand break repair. DNA-binding is sequence-independent but has a high affinity to nicks in double-stranded DNA and to the ends of duplex DNA. Binds to naturally occurring chromosomal ends, and therefore provides chromosomal end protection. Appears to have a role in recruitment of telomerase and CDC13 to the telomere and the subsequent telomere elongation. Required also for telomere recombination to repair telomeric ends in the absence of telomerase. KU70, of the KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA component of telomerase. Involved in telomere maintenance. Interacts with telomeric repeats and subtelomeric sequences thereby controlling telomere length and protecting against subtelomeric rearrangement. Maintains telomeric chromatin, which is involved in silencing the expression of genes located at the telomere. Required for mating-type switching.11 Publications

Miscellaneous

Present with 892 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent DNA helicase activity Source: InterPro
  • damaged DNA binding Source: SGD
  • telomerase RNA binding Source: SGD
  • telomeric DNA binding Source: GO_Central

GO - Biological processi

  • chromatin assembly or disassembly Source: SGD
  • chromatin silencing at silent mating-type cassette Source: SGD
  • chromatin silencing at subtelomere Source: SGD
  • chromatin silencing at telomere Source: SGD
  • double-strand break repair via break-induced replication Source: SGD
  • double-strand break repair via homologous recombination Source: SGD
  • double-strand break repair via nonhomologous end joining Source: SGD
  • establishment of protein-containing complex localization to telomere Source: SGD
  • mitochondrial double-strand break repair via homologous recombination Source: SGD
  • telomere maintenance Source: SGD

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA damage, DNA recombination, DNA repair
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32954-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase II subunit 1 (EC:3.6.4.12)
Alternative name(s):
ATP-dependent DNA helicase II subunit Ku70
High affinity DNA-binding factor subunit 1
Gene namesi
Name:YKU70
Synonyms:HDF1, NES24
Ordered Locus Names:YMR284W
ORF Names:YM8021.10
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR284W
SGDiS000004897 YKU70

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus, Telomere

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002101841 – 602ATP-dependent DNA helicase II subunit 1Add BLAST602

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei370PhosphoserineCombined sources1
Modified residuei371PhosphoserineCombined sources1
Modified residuei372PhosphoserineCombined sources1

Post-translational modificationi

Sumoylated by MMS21.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP32807
PaxDbiP32807
PRIDEiP32807

PTM databases

iPTMnetiP32807

Interactioni

Subunit structurei

Heterodimer of YKU70/HDF1 and YKU80/HDF2.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35464, 347 interactors
ComplexPortaliCPX-1732 Ku70:Ku80 complex
DIPiDIP-2483N
IntActiP32807, 28 interactors
MINTiP32807
STRINGi4932.YMR284W

Structurei

Secondary structure

1602
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi30 – 37Combined sources8
Helixi40 – 43Combined sources4
Helixi47 – 49Combined sources3
Helixi54 – 72Combined sources19
Beta strandi75 – 84Combined sources10
Beta strandi94 – 102Combined sources9
Helixi105 – 119Combined sources15
Helixi125 – 129Combined sources5
Helixi142 – 152Combined sources11
Beta strandi164 – 172Combined sources9
Helixi178 – 180Combined sources3
Helixi183 – 198Combined sources16
Beta strandi201 – 209Combined sources9
Beta strandi211 – 213Combined sources3
Helixi218 – 224Combined sources7
Helixi250 – 252Combined sources3
Helixi253 – 260Combined sources8
Beta strandi266 – 275Combined sources10
Helixi276 – 278Combined sources3
Beta strandi280 – 290Combined sources11
Beta strandi298 – 305Combined sources8
Beta strandi308 – 319Combined sources12
Turni321 – 323Combined sources3
Beta strandi332 – 336Combined sources5
Helixi346 – 354Combined sources9
Beta strandi362 – 370Combined sources9
Helixi371 – 373Combined sources3
Beta strandi385 – 389Combined sources5
Turni391 – 393Combined sources3
Helixi397 – 410Combined sources14
Beta strandi413 – 425Combined sources13
Beta strandi428 – 434Combined sources7
Beta strandi437 – 441Combined sources5
Beta strandi443 – 449Combined sources7
Helixi452 – 454Combined sources3
Helixi471 – 486Combined sources16
Helixi496 – 498Combined sources3
Helixi502 – 515Combined sources14
Helixi528 – 538Combined sources11
Helixi540 – 557Combined sources18
Turni561 – 564Combined sources4
Helixi565 – 583Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5Y58X-ray2.80A/C/E28-602[»]
ProteinModelPortaliP32807
SMRiP32807
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini268 – 483KuAdd BLAST216

Sequence similaritiesi

Belongs to the ku70 family.Curated

Phylogenomic databases

InParanoidiP32807
KOiK10884
OMAiDVFTSCN
OrthoDBiEOG092C18H2

Family and domain databases

Gene3Di2.40.290.10, 1 hit
3.40.50.410, 1 hit
InterProiView protein in InterPro
IPR006165 Ku70
IPR006164 Ku70/Ku80_beta-barrel_dom
IPR005160 Ku_C
IPR005161 Ku_N
IPR016194 SPOC-like_C_dom_sf
IPR036465 vWFA_dom_sf
PANTHERiPTHR12604:SF2 PTHR12604:SF2, 1 hit
PfamiView protein in Pfam
PF02735 Ku, 1 hit
PF03730 Ku_C, 1 hit
PF03731 Ku_N, 1 hit
PIRSFiPIRSF003033 Ku70, 1 hit
SMARTiView protein in SMART
SM00559 Ku78, 1 hit
SUPFAMiSSF100939 SSF100939, 1 hit
SSF53300 SSF53300, 1 hit

Sequencei

Sequence statusi: Complete.

P32807-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSVTNAFGN SGELNDQVDE TGYRKFDIHE GILFCIELSE TMFKESSDLE
60 70 80 90 100
YKSPLLEILE SLDELMSQLV ITRPGTAIGC YFYYCNREDA KEGIYELFPL
110 120 130 140 150
RDINATFMKK LNDLLEDLSS GRISLYDYFM FQQTGSEKQV RLSVLFTFML
160 170 180 190 200
DTFLEEIPGQ KQLSNKRVFL FTDIDKPQEA QDIDERARLR RLTIDLFDNK
210 220 230 240 250
VNFATFFIGY ADKPFDNEFY SDILQLGSHT NENTGLDSEF DGPSTKPIDA
260 270 280 290 300
KYIKSRILRK KEVKRIMFQC PLILDEKTNF IVGVKGYTMY THEKAGVRYK
310 320 330 340 350
LVYEHEDIRQ EAYSKRKFLN PITGEDVTGK TVKVYPYGDL DINLSDSQDQ
360 370 380 390 400
IVMEAYTQKD AFLKIIGFRS SSKSIHYFNN IDKSSFIVPD EAKYEGSIRT
410 420 430 440 450
LASLLKILRK KDKIAILWGK LKSNSHPSLY TLSPSSVKDY NEGFYLYRVP
460 470 480 490 500
FLDEIRKFPS LLSYDDGSEH KLDYDNMKKV TQSIMGYFNL RDGYNPSDFK
510 520 530 540 550
NPLLQKHYKV LHDYLLQIET TFDENETPNT KKDRMMREDD SLRKLYYIRN
560 570 580 590 600
KILESEKSED PIIQRLNKYV KIWNMFYKKF NDDNISIKEE KKPFDKKPKF

NI
Length:602
Mass (Da):70,647
Last modified:February 1, 1996 - v2
Checksum:iBD58160328EE6C6E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3S → P in CAA49840 (PubMed:8509423).Curated1
Sequence conflicti87R → T in AAT92882 (PubMed:9169872).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti21T → K in strain: ABYS 60 and SK1. 1
Natural varianti50E → D in strain: ABYS 60, DBVPG6044, SK1 and YPS128. 1
Natural varianti230T → A in strain: DBVPG6044. 1
Natural varianti368F → I in strain: YPS128. 1
Natural varianti562I → T in strain: ABYS 60, DBVPG6044, SK1 and YPS128. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70379 Genomic DNA Translation: CAA49840.1
D15052 Genomic DNA Translation: BAA03648.1
AM296304 Genomic DNA Translation: CAL36013.1
AM296302 Genomic DNA Translation: CAL36015.1
AM296301 Genomic DNA Translation: CAL36016.1
AM296299 Genomic DNA Translation: CAL36018.1
AM296298 Genomic DNA Translation: CAL36019.1
AM296297 Genomic DNA Translation: CAL36020.1
AM296296 Genomic DNA Translation: CAL36021.1
AM296294 Genomic DNA Translation: CAL36023.1
AM296292 Genomic DNA Translation: CAL36025.1
AM296295 Genomic DNA Translation: CAL36022.1
AM296303 Genomic DNA Translation: CAL36014.1
AM296293 Genomic DNA Translation: CAL36024.1
Z49704 Genomic DNA Translation: CAA89782.1
AY692863 Genomic DNA Translation: AAT92882.1
BK006946 Genomic DNA Translation: DAA10185.1
PIRiS54591
RefSeqiNP_014011.1, NM_001182791.1

Genome annotation databases

EnsemblFungiiYMR284W; YMR284W; YMR284W
GeneIDi855328
KEGGisce:YMR284W

Similar proteinsi

Entry informationi

Entry nameiKU70_YEAST
AccessioniPrimary (citable) accession number: P32807
Secondary accession number(s): D6W0B1
, P32498, Q0P778, Q0P779, Q0P787, Q0P789, Q6B267
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1996
Last modified: June 20, 2018
This is version 166 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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