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P32807

- KU70_YEAST

UniProt

P32807 - KU70_YEAST

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Protein

ATP-dependent DNA helicase II subunit 1

Gene
YKU70, HDF1, NES24, YMR284W, YM8021.10
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Single-stranded DNA-dependent ATP-dependent helicase. Involved in non-homologous end joining (NHEJ) DNA double strand break repair. DNA-binding is sequence-independent but has a high affinity to nicks in double-stranded DNA and to the ends of duplex DNA. Binds to naturally occurring chromosomal ends, and therefore provides chromosomal end protection. Appears to have a role in recruitment of telomerase and CDC13 to the telomere and the subsequent telomere elongation. Required also for telomere recombination to repair telomeric ends in the absence of telomerase. KU70, of the KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA component of telomerase. Involved in telomere maintenance. Interacts with telomeric repeats and subtelomeric sequences thereby controlling telomere length and protecting against subtelomeric rearrangement. Maintains telomeric chromatin, which is involved in silencing the expression of genes located at the telomere. Required for mating-type switching.11 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent DNA helicase activity Source: InterPro
  3. damaged DNA binding Source: SGD
  4. protein binding Source: IntAct
  5. RNA binding Source: SGD
  6. telomeric DNA binding Source: InterPro

GO - Biological processi

  1. chromatin assembly or disassembly Source: SGD
  2. chromatin silencing Source: SGD
  3. chromatin silencing at silent mating-type cassette Source: SGD
  4. double-strand break repair via break-induced replication Source: SGD
  5. double-strand break repair via homologous recombination Source: SGD
  6. double-strand break repair via nonhomologous end joining Source: SGD
  7. mitochondrial double-strand break repair via homologous recombination Source: SGD
  8. telomere maintenance Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32954-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase II subunit 1 (EC:3.6.4.12)
Alternative name(s):
ATP-dependent DNA helicase II subunit Ku70
High affinity DNA-binding factor subunit 1
Gene namesi
Name:YKU70
Synonyms:HDF1, NES24
Ordered Locus Names:YMR284W
ORF Names:YM8021.10
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR284w.
SGDiS000004897. YKU70.

Subcellular locationi

Nucleus. Chromosometelomere 1 Publication

GO - Cellular componenti

  1. Ku70:Ku80 complex Source: SGD
  2. nuclear chromatin Source: SGD
  3. nuclear envelope Source: SGD
  4. nuclear telomeric heterochromatin Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus, Telomere

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 602602ATP-dependent DNA helicase II subunit 1PRO_0000210184Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei370 – 3701Phosphoserine1 Publication
Modified residuei371 – 3711Phosphoserine1 Publication
Modified residuei372 – 3721Phosphoserine1 Publication

Post-translational modificationi

Sumoylated by MMS21.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP32807.
PaxDbiP32807.
PeptideAtlasiP32807.
PRIDEiP32807.

Expressioni

Gene expression databases

GenevestigatoriP32807.

Interactioni

Subunit structurei

Heterodimer of YKU70/HDF1 and YKU80/HDF2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
YKU80Q044373EBI-8214,EBI-8224

Protein-protein interaction databases

BioGridi35464. 323 interactions.
DIPiDIP-2483N.
IntActiP32807. 2 interactions.
MINTiMINT-619653.

Structurei

3D structure databases

ProteinModelPortaliP32807.
SMRiP32807. Positions 30-518.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini268 – 483216KuAdd
BLAST

Sequence similaritiesi

Belongs to the ku70 family.
Contains 1 Ku domain.

Phylogenomic databases

eggNOGiNOG305318.
GeneTreeiENSGT00390000001422.
KOiK10884.
OMAiPPYLKLL.
OrthoDBiEOG747PSQ.

Family and domain databases

Gene3Di1.10.1600.10. 1 hit.
2.40.290.10. 1 hit.
3.40.50.410. 1 hit.
InterProiIPR006165. Ku70.
IPR006164. Ku70/Ku80_beta-barrel_dom.
IPR005160. Ku_C.
IPR005161. Ku_N.
IPR016194. SPOC_like_C_dom.
IPR002035. VWF_A.
[Graphical view]
PANTHERiPTHR12604:SF2. PTHR12604:SF2. 1 hit.
PfamiPF02735. Ku. 1 hit.
PF03730. Ku_C. 1 hit.
PF03731. Ku_N. 1 hit.
[Graphical view]
PIRSFiPIRSF003033. Ku70. 1 hit.
SMARTiSM00559. Ku78. 1 hit.
[Graphical view]
SUPFAMiSSF100939. SSF100939. 1 hit.
SSF53300. SSF53300. 1 hit.

Sequencei

Sequence statusi: Complete.

P32807-1 [UniParc]FASTAAdd to Basket

« Hide

MRSVTNAFGN SGELNDQVDE TGYRKFDIHE GILFCIELSE TMFKESSDLE    50
YKSPLLEILE SLDELMSQLV ITRPGTAIGC YFYYCNREDA KEGIYELFPL 100
RDINATFMKK LNDLLEDLSS GRISLYDYFM FQQTGSEKQV RLSVLFTFML 150
DTFLEEIPGQ KQLSNKRVFL FTDIDKPQEA QDIDERARLR RLTIDLFDNK 200
VNFATFFIGY ADKPFDNEFY SDILQLGSHT NENTGLDSEF DGPSTKPIDA 250
KYIKSRILRK KEVKRIMFQC PLILDEKTNF IVGVKGYTMY THEKAGVRYK 300
LVYEHEDIRQ EAYSKRKFLN PITGEDVTGK TVKVYPYGDL DINLSDSQDQ 350
IVMEAYTQKD AFLKIIGFRS SSKSIHYFNN IDKSSFIVPD EAKYEGSIRT 400
LASLLKILRK KDKIAILWGK LKSNSHPSLY TLSPSSVKDY NEGFYLYRVP 450
FLDEIRKFPS LLSYDDGSEH KLDYDNMKKV TQSIMGYFNL RDGYNPSDFK 500
NPLLQKHYKV LHDYLLQIET TFDENETPNT KKDRMMREDD SLRKLYYIRN 550
KILESEKSED PIIQRLNKYV KIWNMFYKKF NDDNISIKEE KKPFDKKPKF 600
NI 602
Length:602
Mass (Da):70,647
Last modified:February 1, 1996 - v2
Checksum:iBD58160328EE6C6E
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti21 – 211T → K in strain: ABYS 60 and SK1.
Natural varianti50 – 501E → D in strain: ABYS 60, DBVPG6044, SK1 and YPS128.
Natural varianti230 – 2301T → A in strain: DBVPG6044.
Natural varianti368 – 3681F → I in strain: YPS128.
Natural varianti562 – 5621I → T in strain: ABYS 60, DBVPG6044, SK1 and YPS128.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti3 – 31S → P in CAA49840. 1 Publication
Sequence conflicti87 – 871R → T in AAT92882. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X70379 Genomic DNA. Translation: CAA49840.1.
D15052 Genomic DNA. Translation: BAA03648.1.
AM296304 Genomic DNA. Translation: CAL36013.1.
AM296302 Genomic DNA. Translation: CAL36015.1.
AM296301 Genomic DNA. Translation: CAL36016.1.
AM296299 Genomic DNA. Translation: CAL36018.1.
AM296298 Genomic DNA. Translation: CAL36019.1.
AM296297 Genomic DNA. Translation: CAL36020.1.
AM296296 Genomic DNA. Translation: CAL36021.1.
AM296294 Genomic DNA. Translation: CAL36023.1.
AM296292 Genomic DNA. Translation: CAL36025.1.
AM296295 Genomic DNA. Translation: CAL36022.1.
AM296303 Genomic DNA. Translation: CAL36014.1.
AM296293 Genomic DNA. Translation: CAL36024.1.
Z49704 Genomic DNA. Translation: CAA89782.1.
AY692863 Genomic DNA. Translation: AAT92882.1.
BK006946 Genomic DNA. Translation: DAA10185.1.
PIRiS54591.
RefSeqiNP_014011.1. NM_001182791.1.

Genome annotation databases

EnsemblFungiiYMR284W; YMR284W; YMR284W.
GeneIDi855328.
KEGGisce:YMR284W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X70379 Genomic DNA. Translation: CAA49840.1 .
D15052 Genomic DNA. Translation: BAA03648.1 .
AM296304 Genomic DNA. Translation: CAL36013.1 .
AM296302 Genomic DNA. Translation: CAL36015.1 .
AM296301 Genomic DNA. Translation: CAL36016.1 .
AM296299 Genomic DNA. Translation: CAL36018.1 .
AM296298 Genomic DNA. Translation: CAL36019.1 .
AM296297 Genomic DNA. Translation: CAL36020.1 .
AM296296 Genomic DNA. Translation: CAL36021.1 .
AM296294 Genomic DNA. Translation: CAL36023.1 .
AM296292 Genomic DNA. Translation: CAL36025.1 .
AM296295 Genomic DNA. Translation: CAL36022.1 .
AM296303 Genomic DNA. Translation: CAL36014.1 .
AM296293 Genomic DNA. Translation: CAL36024.1 .
Z49704 Genomic DNA. Translation: CAA89782.1 .
AY692863 Genomic DNA. Translation: AAT92882.1 .
BK006946 Genomic DNA. Translation: DAA10185.1 .
PIRi S54591.
RefSeqi NP_014011.1. NM_001182791.1.

3D structure databases

ProteinModelPortali P32807.
SMRi P32807. Positions 30-518.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35464. 323 interactions.
DIPi DIP-2483N.
IntActi P32807. 2 interactions.
MINTi MINT-619653.

Proteomic databases

MaxQBi P32807.
PaxDbi P32807.
PeptideAtlasi P32807.
PRIDEi P32807.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR284W ; YMR284W ; YMR284W .
GeneIDi 855328.
KEGGi sce:YMR284W.

Organism-specific databases

CYGDi YMR284w.
SGDi S000004897. YKU70.

Phylogenomic databases

eggNOGi NOG305318.
GeneTreei ENSGT00390000001422.
KOi K10884.
OMAi PPYLKLL.
OrthoDBi EOG747PSQ.

Enzyme and pathway databases

BioCyci YEAST:G3O-32954-MONOMER.

Miscellaneous databases

NextBioi 979046.
PROi P32807.

Gene expression databases

Genevestigatori P32807.

Family and domain databases

Gene3Di 1.10.1600.10. 1 hit.
2.40.290.10. 1 hit.
3.40.50.410. 1 hit.
InterProi IPR006165. Ku70.
IPR006164. Ku70/Ku80_beta-barrel_dom.
IPR005160. Ku_C.
IPR005161. Ku_N.
IPR016194. SPOC_like_C_dom.
IPR002035. VWF_A.
[Graphical view ]
PANTHERi PTHR12604:SF2. PTHR12604:SF2. 1 hit.
Pfami PF02735. Ku. 1 hit.
PF03730. Ku_C. 1 hit.
PF03731. Ku_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF003033. Ku70. 1 hit.
SMARTi SM00559. Ku78. 1 hit.
[Graphical view ]
SUPFAMi SSF100939. SSF100939. 1 hit.
SSF53300. SSF53300. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A putative homologue of the human autoantigen Ku from Saccharomyces cerevisiae."
    Feldmann H., Winnacker E.L.
    J. Biol. Chem. 268:12895-12900(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 4-20, SUBUNIT.
    Strain: ABYS 60.
  2. "Cloning and sequencing of the NES24 gene of Saccharomyces cerevisiae."
    Yoshida M., Shimma Y., Uno I., Toh-e A.
    Yeast 10:371-376(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Sequence diversity, reproductive isolation and species concepts in Saccharomyces."
    Liti G., Barton D.B., Louis E.J.
    Genetics 174:839-850(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DBVPG1135, DBVPG1373, DBVPG1378, DBVPG1788, DBVPG1794, DBVPG3051, DBVPG6044, DBVPG6763, DBVPG6765, SK1 and Y55.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  7. "Involvement of the Saccharomyces cerevisiae HDF1 gene in DNA double-strand break repair and recombination."
    Mages G.J., Feldmann H.M., Winnacker E.-L.
    J. Biol. Chem. 271:7910-7915(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN TELOMERE MAINTENANCE AND MATING-TYPE SWITCHING.
  8. "Hdf1, a yeast Ku-protein homologue, is involved in illegitimate recombination, but not in homologous recombination."
    Tsukamoto Y., Kato J., Ideka H.
    Nucleic Acids Res. 24:2067-2072(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Telomerase, Ku, and telomeric silencing in Saccharomyces cerevisiae."
    Evans S.K., Sistrunk M.L., Nugent C.I., Lundblad V.
    Chromosoma 107:352-358(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN TELOMERIC GENE SILENCING.
  10. "Mutation of yeast Ku genes disrupts the subnuclear organization of telomeres."
    Laroche T., Martin S.G., Gotta M., Gorham H.C., Pryde F.E., Louis E.J., Gasser S.M.
    Curr. Biol. 8:653-656(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN TELOMERE MAINTENANCE, SUBCELLULAR LOCATION.
  11. "Telomere maintenance is dependent on activities required for end repair of double-strand breaks."
    Nugent C.I., Bosco G., Ross L.O., Evans S.K., Salinger A.P., Moore J.K., Haber J.E., Lundblad V.
    Curr. Biol. 8:657-660(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN DOUBLE-STRAND DNA REPAIR AND TELOMERE MAINTENANCE.
  12. "The yeast Ku heterodimer is essential for protection of the telomere against nucleolytic and recombinational activities."
    Polotnianka R.M., Li J., Lustig A.J.
    Curr. Biol. 8:831-834(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN TELOMERE MAINTENANCE.
  13. "The Saccharomyces cerevisiae DNA damage checkpoint is required for efficient repair of double strand breaks by non-homologous end joining."
    de la Torre-Ruiz M., Lowndes N.F.
    FEBS Lett. 467:311-315(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN NON-HOMOLOGOUS END JOINING DNA REPAIR.
  14. "Cdc13 cooperates with the yeast Ku proteins and Stn1 to regulate telomerase recruitment."
    Grandin N., Damon C., Charbonneau M.
    Mol. Cell. Biol. 20:8397-8408(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN TELOMERASE AND CDC13 TELOMERE RECRUITMENT.
  15. "Involvement of replicative polymerases, Tel1p, Mec1p, Cdc13p, and the Ku complex in telomere-telomere recombination."
    Tsai Y.-L., Tseng S.-F., Chang S.-H., Lin C.-C., Teng S.-C.
    Mol. Cell. Biol. 22:5679-5687(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN TELOMERE RECOMBINATION.
  16. "Ku interacts with telomerase RNA to promote telomere addition at native and broken chromosome ends."
    Stellwagen A.E., Haimberger Z.W., Veatch J.R., Gottschling D.E.
    Genes Dev. 17:2384-2395(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN TELOMERIC REPAIR AND BINDING TO TLC1 STEM LOOP.
  17. "The Ku heterodimer performs separable activities at double-strand breaks and chromosome termini."
    Bertuch A.A., Lundblad V.
    Mol. Cell. Biol. 23:8202-8215(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN DOUBLE-STRAND DNA REPAIR AND TELOMERE MAINTENANCE.
  18. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  19. "A SUMO ligase is part of a nuclear multiprotein complex that affects DNA repair and chromosomal organization."
    Zhao X., Blobel G.
    Proc. Natl. Acad. Sci. U.S.A. 102:4777-4782(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUMOYLATION BY MMS21.
  20. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-370; SER-371 AND SER-372, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiKU70_YEAST
AccessioniPrimary (citable) accession number: P32807
Secondary accession number(s): D6W0B1
, P32498, Q0P778, Q0P779, Q0P787, Q0P789, Q6B267
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 892 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

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