Reviewed, UniProtKB/Swiss-Prot P32805 (FZF1_YEAST)

Names and origin

Protein names

Recommended name:
    Zinc finger protein FZF1
Alternative name(s):
    Sulfite resistance protein 1

Gene names

Name:	FZF1
Synonyms:	SUL1
Ordered Locus Names:	YGL254W
ORF Names:	NCR299

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	299 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May function as a transcription factor.
Subcellular location	Nucleus Potential.
Miscellaneous	Present with 2120 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Contains 5 C2H2-type zinc fingers.
Sequence caution	The sequence CAA47998.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay. Source: SGD sulfite transport Traceable author statement. Source: SGD transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred by curator. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct sequence-specific DNA binding Inferred from direct assay. Source: SGD transcription factor activity Inferred from direct assay. Source: SGD zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
LYS5	P50113	1	EBI-7201,EBI-10282

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 299	299	Zinc finger protein FZF1		PRO_0000046805
Regions
	Zinc finger	12 – 36	25	C2H2-type 1
	Zinc finger	42 – 66	25	C2H2-type 2
	Zinc finger	72 – 94	23	C2H2-type 3
	Zinc finger	155 – 179	25	C2H2-type 4
	Zinc finger	246 – 271	26	C2H2-type 5
	Motif	96 – 107	12	Nuclear localization signal Potential
Natural variations
	Natural variant	110	1	G → E in strain: Sgu52E and Sgu52F.
	Natural variant	120	1	N → D in strain: MMW1-15h2, MMW1-2h2, Sgu52E and Sgu52F.
	Natural variant	228	1	V → I in strain: MMW1-15h2, MMW1-2h2, Sgu52E and Sgu52F.
	Natural variant	238	1	D → N in strain: MMW1-15h2 and MMW1-2h2.
Experimental info
	Sequence conflict	180	1	H → D in CAA54996. Ref.2
	Sequence conflict	235	1	T → W Ref.1
	Sequence conflict	246	1	N → Y in CAA54996. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P32805-1 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: A4E7257F012B32FF	FASTA	299	33,994
	10 20 30 40 50 60 MTDIGRTKSR NYKCSFDGCE KVYNRPSLLQ QHQNSHTNQK PYHCDEPGCG KKFIRPCHLR 70 80 90 100 110 120 VHKWTHSQIK PKACTLCQKR FVTNQQLRRH LNSHERKSKL ASRIDRKHEG VNANVKAELN 130 140 150 160 170 180 GKEGGFDPKL PSGSPMCGEE FSQGHLPGYD DMQVLQCPYK SCQKVTSFND DLINHMLQHH 190 200 210 220 230 240 IASKLVVPSG DPSLKESLPT SEKSSSTDTT SIPQLSFSTT GTSSSESVDS TTAQTPTDPE 250 260 270 280 290 SYWSDNRCKH SDCQELSPFA SVFDLIDHYD HTHAFIPETL VKYSYIHLYK PSVWDLFEY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a gene encoding a novel zinc finger protein in Saccharomyces cerevisiae." Breitwieser W., Price C., Schuster T. Yeast 9:551-556(1993) [PubMed: 8322518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 200060 / W303.
[2]	"Cloning and characterization of a sulphite-resistance gene of Saccharomyces cerevisiae." Casalone E., Colella C.M., Daly S., Fontana S., Torricelli I., Polsinelli M. Yeast 10:1101-1110(1994) [PubMed: 7992510] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Population structure and gene evolution in Saccharomyces cerevisiae." Aa E., Townsend J.P., Adams R.I., Nielsen K.M., Taylor J.W. FEMS Yeast Res. 6:702-715(2006) [PubMed: 16879422] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 76625 / YPH499, MMW1-15, MMW1-15h2, MMW1-2, MMW1-2h2, Sgu52E, Sgu52F, YPS396, YPS400, YPS598, YPS600, YPS602, YPS604, YPS606, YPS608 and YPS610.
[4]	"Sequence of a 39,411 bp DNA fragment covering the left end of chromosome VII of Saccharomyces cerevisiae." Coissac E., Maillier E., Robineau S., Netter P. Yeast 12:1555-1562(1996) [PubMed: 8972578] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X67787 Genomic DNA. Translation: CAA47998.1. Sequence problems. X78104 Genomic DNA. Translation: CAA54996.1. AY949902 Genomic DNA. Translation: AAY27304.1. AY949903 Genomic DNA. Translation: AAY27305.1. AY949905 Genomic DNA. Translation: AAY27307.1. AY949906 Genomic DNA. Translation: AAY27308.1. AY949908 Genomic DNA. Translation: AAY27310.1. AY949909 Genomic DNA. Translation: AAY27311.1. AY949910 Genomic DNA. Translation: AAY27312.1. AY949911 Genomic DNA. Translation: AAY27313.1. AY949912 Genomic DNA. Translation: AAY27314.1. AY949913 Genomic DNA. Translation: AAY27315.1. AY949914 Genomic DNA. Translation: AAY27316.1. AY949915 Genomic DNA. Translation: AAY27317.1. AY949916 Genomic DNA. Translation: AAY27318.1. AY949917 Genomic DNA. Translation: AAY27319.1. AY949918 Genomic DNA. Translation: AAY27320.1. AY949919 Genomic DNA. Translation: AAY27321.1. X94357 Genomic DNA. Translation: CAA64133.1. Z72776 Genomic DNA. Translation: CAA96974.1.
PIR	S61607.
RefSeq	NP_011260.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1324N.
IntAct	P32805. 9 interactions.
STRING	P32805.
Genome annotation databases
Ensembl	YGL254W; YGL254W; YGL254W; Saccharomyces cerevisiae. [Genome view]
GeneID	852638.
KEGG	sce:YGL254W.
NMPDR	fig|4932.3.peg.2363.
Organism-specific databases
CYGD	YGL254w.
SGD	S000003223. FZF1.
Phylogenomic databases
HOGENOM	P32805.
OMA	RVHKWTH
OrthoDB	EOG9GMWFD
Gene expression databases
ArrayExpress	P32805.
Genevestigator	P32805.
GermOnline	YGL254W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. [Graphical view]
Pfam	PF00096. zf-C2H2. 4 hits. [Graphical view]
SMART	SM00355. ZnF_C2H2. 5 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 3 hits. PS50157. ZINC_FINGER_C2H2_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	971885.

Entry information

Entry name

FZF1_YEAST

Accession

Primary (citable) accession number: P32805
Secondary accession number(s): Q2VQB9, Q2VQC2, Q2VQC3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1996
Last modified:	November 24, 2009
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)