Reviewed, UniProtKB/Swiss-Prot P32804 (ZRT1_YEAST)

Names and origin

Protein names

Recommended name:
    Zinc-regulated transporter 1
Alternative name(s):
    High-affinity zinc transport protein ZRT1

Gene names

Name:	ZRT1
Ordered Locus Names:	YGL255W
ORF Names:	NRC376

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	High-affinity zinc transport protein. Ref.4
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Induction	Induced in activity >100-fold in response to zinc-limiting growth conditions. Not expressed in zinc-replete cells. Ref.4
Miscellaneous	Inhibited by Cu2+ and Fe3+ ions.
Sequence similarities	Belongs to the ZIP transporter (TC 2.A.5) family. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport Zinc transport
Cellular component	Membrane
Domain	Transmembrane
Ligand	Zinc
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	high-affinity zinc ion transport Inferred from mutant phenotype. Source: SGD
Cellular component	integral to plasma membrane Inferred from mutant phenotype. Source: SGD
Molecular function	high affinity zinc uptake transmembrane transporter activity Inferred from mutant phenotype. Source: SGD zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Zinc-regulated transporter 1		PRO_0000068768
Regions
	Topological domain	1 – 50	50	Extracellular Potential
	Transmembrane	51 – 71	21	Potential
	Topological domain	72 – 80	9	Cytoplasmic Potential
	Transmembrane	81 – 101	21	Potential
	Topological domain	102 – 122	21	Extracellular Potential
	Transmembrane	123 – 143	21	Potential
	Topological domain	144 – 216	73	Cytoplasmic Potential
	Transmembrane	217 – 237	21	Potential
	Topological domain	238 – 242	5	Extracellular Potential
	Transmembrane	243 – 263	21	Potential
	Topological domain	264 – 278	15	Cytoplasmic Potential
	Transmembrane	279 – 299	21	Potential
	Topological domain	300 – 310	11	Extracellular Potential
	Transmembrane	311 – 331	21	Potential
	Topological domain	332 – 354	23	Cytoplasmic Potential
	Transmembrane	355 – 375	21	Potential
	Topological domain	376	1	Extracellular Potential
	Region	232 – 233	2	Heavy metals binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	P32804-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 7A1F8367D49BAC3C	FASTA	376	41,582
	10 20 30 40 50 60 MSNVTTPWWK QWDPSEVTLA DKTPDDVWKT CVLQGVYFGG NEYNGNLGAR ISSVFVILFV 70 80 90 100 110 120 STFFTMFPLI STKVKRLRIP LYVYLFAKYF GSGVIVATAF IHLMDPAYGA IGGTTCVGQT 130 140 150 160 170 180 GNWGLYSWCP AIMLTSLTFT FLTDLFSSVW VERKYGLSHD HTHDEIKDTV VRNTAAVSSE 190 200 210 220 230 240 NDNENGTANG SHDTKNGVEY YEDSDATSMD VVQSFQAQFY AFLILEFGVI FHSVMIGLNL 250 260 270 280 290 300 GSVGDEFSSL YPVLVFHQSF EGLGIGARLS AIEFPRSKRW WPWALCVAYG LTTPICVAIG 310 320 330 340 350 360 LGVRTRYVSG SYTALVISGV LDAISAGILL YTGLVELLAR DFIFNPQRTK DLRELSFNVI 370 CTLFGAGIMA LIGKWA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a gene encoding a novel zinc finger protein in Saccharomyces cerevisiae." Breitwieser W., Price C., Schuster T. Yeast 9:551-556(1993) [PubMed: 8322518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 200060 / W303.
[2]	"Sequence of a 39,411 bp DNA fragment covering the left end of chromosome VII of Saccharomyces cerevisiae." Coissac E., Maillier E., Robineau S., Netter P. Yeast 12:1555-1562(1996) [PubMed: 8972578] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"The ZRT2 gene encodes the low affinity zinc transporter in Saccharomyces cerevisiae." Zhao H., Eide D. J. Biol. Chem. 271:23203-23210(1996) [PubMed: 8798516] [Abstract] Cited for: FUNCTION, INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X67787 Genomic DNA. Translation: CAA47997.1. Z72777 Genomic DNA. Translation: CAA96975.1. X94357 Genomic DNA. Translation: CAA64132.1.
PIR	S33654.
RefSeq	NP_011259.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P32804. 1 interaction.
STRING	P32804.
Protein family/group databases
TCDB	2.A.5.1.1. zinc (Zn2+)-iron (Fe2+) permease (ZIP) family.
Genome annotation databases
Ensembl	YGL255W; YGL255W; YGL255W; Saccharomyces cerevisiae. [Genome view]
GeneID	852637.
KEGG	sce:YGL255W.
NMPDR	fig|4932.3.peg.2362.
Organism-specific databases
CYGD	YGL255w.
SGD	S000003224. ZRT1.
Phylogenomic databases
HOGENOM	P32804.
OMA	WIAERWT
OrthoDB	EOG9S7M6P
Gene expression databases
ArrayExpress	P32804.
Genevestigator	P32804.
GermOnline	YGL255W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003689. ZIP. IPR004698. Zn/Fe_permease_fun/pln. [Graphical view]
Pfam	PF02535. Zip. 1 hit. [Graphical view]
TIGRFAMs	TIGR00820. zip. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	971882.

Entry information

Entry name

ZRT1_YEAST

Accession

Primary (citable) accession number: P32804

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	November 24, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)