P32803 (EMP24_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endosomal protein P24B

Alternative name(s):
24 kDa endomembrane protein
Basic 24 kDa late endocytic intermediate component

Gene names

Name:	EMP24
Ordered Locus Names:	YGL200C
ORF Names:	G1271

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	203 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Constituent of COPII-coated endoplasmic reticulum-derived transport vesicles. Required for efficient transport of a subset of secretory proteins to the Golgi. Ref.10
Subunit structure	Associates with ERV25, ERP1 and ERP2. Interacts also with SAR1, SEC23 and SEC24. Ref.7 Ref.8 Ref.10
Subcellular location	Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Note: Recycles between endoplasmic reticulum and Golgi. Ref.9 Ref.10
Miscellaneous	Present with 26800 molecules/cell in log phase SD medium. Ref.11
Sequence similarities	Belongs to the EMP24/GP25L family. Contains 1 GOLD domain.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Domain	Signal Transmembrane Transmembrane helix
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	ER to Golgi vesicle-mediated transport Inferred from mutant phenotype. Source: SGD protein retention in ER lumen Inferred from mutant phenotype. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW vesicle organization Inferred from mutant phenotype. Source: SGD
Cellular component	ER to Golgi transport vesicle Inferred from direct assay. Source: SGD Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Ref.6 Ref.9
	Chain	21 – 203	183	Endosomal protein P24B		PRO_0000010407
Regions
	Topological domain	21 – 172	152	Lumenal Potential
	Transmembrane	173 – 193	21	Helical; Potential
	Topological domain	194 – 203	10	Cytoplasmic Potential
	Domain	30 – 118	89	GOLD
Experimental info
	Mutagenesis	202 – 203	2	LV → AA: No change in COPII-binding. Ref.10
	Sequence conflict	32	1	C → H AA sequence Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P32803 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 9B9E46982FA62B07	FASTA	203	23,332
	10 20 30 40 50 60 MASFATKFVI ACFLFFSASA HNVLLPAYGR RCFFEDLSKG DELSISFQFG DRNPQSSSQL 70 80 90 100 110 120 TGDFIIYGPE RHEVLKTVRD TSHGEITLSA PYKGHFQYCF LNENTGIETK DVTFNIHGVV 130 140 150 160 170 180 YVDLDDPNTN TLDSAVRKLS KLTREVKDEQ SYIVIRERTH RNTAESTNDR VKWWSIFQLG 190 200 VVIANSLFQI YYLRRFFEVT SLV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The absence of Emp24p, a component of ER-derived COPII-coated vesicles, causes a defect in transport of selected proteins to the Golgi." Schimmoeller F., Singer-Krueger B., Schroeder S., Krueger U., Barlowe C., Riezman H. EMBO J. 14:1329-1339(1995) [PubMed: 7729411] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Bjourson A.J., McReynolds A.D.K., Wright L.F. Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae." Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V. Yeast 13:55-64(1997) [PubMed: 9046087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-169. Strain: ATCC 96604 / S288c / FY1679.
[6]	"Partial purification and characterization of early and late endosomes from yeast. Identification of four novel proteins." Singer-Krueger B., Frank R., Crausaz F., Riezman H. J. Biol. Chem. 268:14376-14386(1993) [PubMed: 8314797] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-37. Strain: RH732.
[7]	"Erv25p, a component of COPII-coated vesicles, forms a complex with Emp24p that is required for efficient endoplasmic reticulum to Golgi transport." Belden W.J., Barlowe C. J. Biol. Chem. 271:26939-26946(1996) [PubMed: 8900179] [Abstract] Cited for: INTERACTION WITH ERV25.
[8]	"Erp1p and Erp2p, partners for Emp24p and Erv25p in a yeast p24 complex." Marzioch M., Henthorn D.C., Herrmann J.M., Wilson R., Thomas D.Y., Bergeron J.J.M., Solari R.C., Rowley A. Mol. Biol. Cell 10:1923-1938(1999) [PubMed: 10359606] [Abstract] Cited for: INTERACTION WITH ERV25; ERP1 AND ERP2.
[9]	"Proteins in the early Golgi compartment of Saccharomyces cerevisiae immunoisolated by Sed5p." Cho J.-H., Noda Y., Yoda K. FEBS Lett. 469:151-154(2000) [PubMed: 10713261] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-25, SUBCELLULAR LOCATION.
[10]	"Distinct roles for the cytoplasmic tail sequences of Emp24p and Erv25p in transport between the endoplasmic reticulum and Golgi complex." Belden W.J., Barlowe C. J. Biol. Chem. 276:43040-43048(2001) [PubMed: 11560939] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SAR1; SEC23 AND SEC24, MUTAGENESIS OF 202-LYS-VAL-203.
[11]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[12]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X67317 Genomic DNA. Translation: CAA47731.1. Z72722 Genomic DNA. Translation: CAA96912.1. X91837 Genomic DNA. Translation: CAA62944.1. BK006941 Genomic DNA. Translation: DAA07915.1.
PIR	S25109.
RefSeq	NP_011315.1. NM_001181065.1.
3D structure databases
ProteinModelPortal	P32803.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2856N.
IntAct	P32803. 18 interactions.
MINT	MINT-663685.
STRING	P32803.
Proteomic databases
PeptideAtlas	P32803.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL200C; YGL200C; YGL200C.
GeneID	852675.
KEGG	sce:YGL200C.
NMPDR	fig|4932.3.peg.2418.
Organism-specific databases
CYGD	YGL200c.
SGD	S000003168. EMP24.
Phylogenomic databases
eggNOG	fuNOG05112.
GeneTree	EFGT00070000008733.
HOGENOM	HBG333344.
OMA	LDIDFWI.
OrthoDB	EOG4KD9WM.
Gene expression databases
ArrayExpress	P32803.
Genevestigator	P32803.
GermOnline	YGL200C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR009038. GOLD. [Graphical view]
Pfam	PF01105. EMP24_GP25L. 1 hit. [Graphical view]
SUPFAM	SSF101576. GOLD. 1 hit.
PROSITE	PS50866. GOLD. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971981.

Entry information

Entry name

EMP24_YEAST

Accession

Primary (citable) accession number: P32803
Secondary accession number(s): D6VTV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	January 25, 2012
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program