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Protein

Endosomal protein P24B

Gene

EMP24

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Constituent of COPII-coated endoplasmic reticulum-derived transport vesicles. Required for efficient transport of a subset of secretory proteins to the Golgi.1 Publication

Miscellaneous

Present with 26800 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • ER to Golgi vesicle-mediated transport Source: SGD
  • protein retention in ER lumen Source: SGD
  • protein transport Source: UniProtKB-KW
  • vesicle organization Source: SGD

Keywordsi

Biological processER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30680-MONOMER.
ReactomeiR-SCE-166016. Toll Like Receptor 4 (TLR4) Cascade.
R-SCE-6798695. Neutrophil degranulation.
R-SCE-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Endosomal protein P24B
Alternative name(s):
24 kDa endomembrane protein
Basic 24 kDa late endocytic intermediate component
Gene namesi
Name:EMP24
Ordered Locus Names:YGL200C
ORF Names:G1271
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL200C.
SGDiS000003168. EMP24.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 172LumenalSequence analysisAdd BLAST152
Transmembranei173 – 193HelicalSequence analysisAdd BLAST21
Topological domaini194 – 203CytoplasmicSequence analysis10

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi202 – 203LV → AA: No change in COPII-binding. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 202 PublicationsAdd BLAST20
ChainiPRO_000001040721 – 203Endosomal protein P24BAdd BLAST183

Proteomic databases

MaxQBiP32803.
PRIDEiP32803.
TopDownProteomicsiP32803.

Interactioni

Subunit structurei

Associates with ERV25, ERP1 and ERP2. Interacts also with SAR1, SEC23 and SEC24.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33058. 203 interactors.
DIPiDIP-2856N.
ELMiP32803.
IntActiP32803. 22 interactors.
MINTiMINT-663685.
STRINGi4932.YGL200C.

Structurei

3D structure databases

ProteinModelPortaliP32803.
SMRiP32803.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 118GOLDPROSITE-ProRule annotationAdd BLAST89

Sequence similaritiesi

Belongs to the EMP24/GP25L family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00670000097656.
HOGENOMiHOG000160229.
InParanoidiP32803.
KOiK20347.
OMAiYTYCFGN.
OrthoDBiEOG092C4JGT.

Family and domain databases

InterProiView protein in InterPro
IPR009038. GOLD_dom.
IPR036598. GOLD_dom_sf.
IPR015720. TMP21-related.
PANTHERiPTHR22811. PTHR22811. 1 hit.
PfamiView protein in Pfam
PF01105. EMP24_GP25L. 1 hit.
SMARTiView protein in SMART
SM01190. EMP24_GP25L. 1 hit.
SUPFAMiSSF101576. SSF101576. 1 hit.
PROSITEiView protein in PROSITE
PS50866. GOLD. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32803-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASFATKFVI ACFLFFSASA HNVLLPAYGR RCFFEDLSKG DELSISFQFG
60 70 80 90 100
DRNPQSSSQL TGDFIIYGPE RHEVLKTVRD TSHGEITLSA PYKGHFQYCF
110 120 130 140 150
LNENTGIETK DVTFNIHGVV YVDLDDPNTN TLDSAVRKLS KLTREVKDEQ
160 170 180 190 200
SYIVIRERTH RNTAESTNDR VKWWSIFQLG VVIANSLFQI YYLRRFFEVT

SLV
Length:203
Mass (Da):23,332
Last modified:October 1, 1993 - v1
Checksum:i9B9E46982FA62B07
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti32C → H AA sequence (PubMed:8314797).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67317 Genomic DNA. Translation: CAA47731.1.
Z72722 Genomic DNA. Translation: CAA96912.1.
X91837 Genomic DNA. Translation: CAA62944.1.
BK006941 Genomic DNA. Translation: DAA07915.1.
PIRiS25109.
RefSeqiNP_011315.3. NM_001181065.3.

Genome annotation databases

EnsemblFungiiYGL200C; YGL200C; YGL200C.
GeneIDi852675.
KEGGisce:YGL200C.

Similar proteinsi

Entry informationi

Entry nameiEMP24_YEAST
AccessioniPrimary (citable) accession number: P32803
Secondary accession number(s): D6VTV4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 22, 2017
This is version 161 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names