P32800 (PEX2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peroxisomal biogenesis factor 2

Alternative name(s):
Peroxin-2
Peroxisomal protein PAS5
Protein CRT1

Gene names

Name:	PEX2
Synonyms:	CRT1, PAS5
Ordered Locus Names:	YJL210W
ORF Names:	J0240, HRA271

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the import of several proteins into peroxisomes.
Subcellular location	Peroxisome.
Miscellaneous	Present with 339 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the pex2/pex10/pex12 family. Contains 1 RING-type zinc finger.

Ontologies

Keywords
Cellular component	Peroxisome
Coding sequence diversity	Alternative initiation
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein import into peroxisome matrix Inferred from mutant phenotype. Source: SGD
Cellular component	peroxisomal membrane Inferred from direct assay. Source: SGD
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform Long (identifier: P32800-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P32800-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-46: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	Peroxisomal biogenesis factor 2		PRO_0000030724
Regions
	Zinc finger	222 – 259	38	RING-type
Natural variations
	Alternative sequence	1 – 46	46	Missing in isoform Short.		VSP_018874

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified October 1, 1993. Version 1. Checksum: FAEFA3A526E82F3E	FASTA	271	30,752
	10 20 30 40 50 60 MSRVAQLDSI ALDKELYGQF WSEFNAAFNT SEHKEEWELA LNTVVFMCAT RFLPHYGSSC 70 80 90 100 110 120 TYGSALSGVV FQCRKRTLYV VTVLAGYVWK KITHIIFNGP HCGNQMMWLK LYKWVNLLYH 130 140 150 160 170 180 GCDVTNFLRF LAAEGPNARA FLSPLYRAFN VHSTRLIRDG SAIASEFYSN SVFAGLEYQN 190 200 210 220 230 240 RQLLWNALLE LFSNTLLTKR GLLTFVKKPP RSRSTTTYKT VCPRCGGFPT NPYQIACCRA 250 260 270 NYCYVCVVKA LEWSMCDACG SSGRLTASPV Y
	Isoform Short [UniParc]. Checksum: 357856560BB14E33	FASTA	225	25,404
	10 20 30 40 50 60 MCATRFLPHY GSSCTYGSAL SGVVFQCRKR TLYVVTVLAG YVWKKITHII FNGPHCGNQM 70 80 90 100 110 120 MWLKLYKWVN LLYHGCDVTN FLRFLAAEGP NARAFLSPLY RAFNVHSTRL IRDGSAIASE 130 140 150 160 170 180 FYSNSVFAGL EYQNRQLLWN ALLELFSNTL LTKRGLLTFV KKPPRSRSTT TYKTVCPRCG 190 200 210 220 GFPTNPYQIA CCRANYCYVC VVKALEWSMC DACGSSGRLT ASPVY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Independent regulation of full-length and 5'-truncated PAS5 mRNAs in Saccharomyces cerevisiae." Liu Y., Gu K.L., Dieckmann C.L. Yeast 12:135-143(1996) [PubMed: 8686377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of a 40.2 kb DNA fragment located near the left telomere of yeast chromosome X." Vandenbol M., Durand P., Bolle P.-A., Dion C., Portetelle D., Hilger F. Yeast 10:1657-1662(1994) [PubMed: 7725802] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M86538 Genomic DNA. Translation: AAB36836.1. Z34098 Genomic DNA. Translation: CAA84000.1. Z49486 Genomic DNA. Translation: CAA89508.1. BK006943 Genomic DNA. Translation: DAA08600.1.
PIR	S27422.
RefSeq	NP_012325.1. NM_001181643.1.
3D structure databases
ProteinModelPortal	P32800.
SMR	P32800. Positions 217-263.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4760N.
MINT	MINT-551616.
STRING	P32800.
Proteomic databases
PeptideAtlas	P32800.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL210W; YJL210W; YJL210W.
GeneID	853219.
KEGG	sce:YJL210W.
NMPDR	fig|4932.3.peg.3288.
Organism-specific databases
CYGD	YJL210w.
SGD	S000003746. PEX2.
Phylogenomic databases
eggNOG	fuNOG10005.
HOGENOM	HBG202179.
OMA	MSRVAQL.
OrthoDB	EOG466ZWM.
Gene expression databases
ArrayExpress	P32800.
Genevestigator	P32800.
GermOnline	YJL210W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006845. Pex_N. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Gene3D	G3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 1 hit.
KO	K06664.
Pfam	PF04757. Pex2_Pex12. 1 hit. [Graphical view]
PROSITE	PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	973416.

Entry information

Entry name

PEX2_YEAST

Accession

Primary (citable) accession number: P32800
Secondary accession number(s): D6VVY4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	December 14, 2011
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program