UniProtKB - P32798 (COT1_YEAST)
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Protein
Cobalt uptake protein COT1
Gene
COT1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Probably responsible for the uptake of cobalt ions. It appears to act in a dosage-dependent manner to counteract the adverse effects of cobalt ions on cells. It may participate in the regulation of cobalt levels under normal physiological conditions and may be important in the supply of metal that is required for metalloenzyme or cofactor synthesis. It reduces the toxicity of cobalt and rhodium ions. Other components responsible for cobalt transport exist.
Miscellaneous
Present with 2070 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- cobalt ion transmembrane transporter activity Source: SGD
- zinc ion transmembrane transporter activity Source: SGD
GO - Biological processi
- cellular zinc ion homeostasis Source: SGD
- cobalt ion transport Source: SGD
- regulation of sequestering of zinc ion Source: GO_Central
- response to zinc ion Source: GO_Central
- zinc II ion transport Source: SGD
Keywordsi
Biological process | Transport |
Ligand | Cobalt |
Enzyme and pathway databases
BioCyci | YEAST:G3O-33798-MONOMER. |
Reactomei | R-SCE-264876. Insulin processing. R-SCE-425410. Metal ion SLC transporters. R-SCE-435368. Zinc efflux and compartmentalization by the SLC30 family. |
Protein family/group databases
TCDBi | 2.A.4.2.1. the cation diffusion facilitator (cdf) family. |
Names & Taxonomyi
Protein namesi | Recommended name: Cobalt uptake protein COT1 |
Gene namesi | Name:COT1 Ordered Locus Names:YOR316C ORF Names:O6131 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YOR316C. |
SGDi | S000005843. COT1. |
Subcellular locationi
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 10 – 27 | HelicalSequence analysisAdd BLAST | 18 | |
Transmembranei | 43 – 60 | HelicalSequence analysisAdd BLAST | 18 | |
Transmembranei | 78 – 100 | HelicalSequence analysisAdd BLAST | 23 | |
Transmembranei | 114 – 133 | HelicalSequence analysisAdd BLAST | 20 | |
Transmembranei | 244 – 265 | HelicalSequence analysisAdd BLAST | 22 | |
Transmembranei | 279 – 295 | HelicalSequence analysisAdd BLAST | 17 |
Keywords - Cellular componenti
Membrane, MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000206102 | 1 – 439 | Cobalt uptake protein COT1Add BLAST | 439 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 225 | PhosphoserineCombined sources | 1 | |
Cross-linki | 301 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
MaxQBi | P32798. |
PaxDbi | P32798. |
PRIDEi | P32798. |
TopDownProteomicsi | P32798. |
PTM databases
iPTMneti | P32798. |
Interactioni
Binary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
FTH1 | P38310 | 3 | EBI-5006,EBI-20959 |
Protein-protein interaction databases
BioGridi | 34703. 40 interactors. |
DIPi | DIP-7554N. |
IntActi | P32798. 15 interactors. |
MINTi | P32798. |
STRINGi | 4932.YOR316C. |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 140 – 148 | His-rich (could be involved in coordination of cobalt ions) | 9 | |
Compositional biasi | 163 – 169 | His-rich (could be involved in coordination of cobalt ions) | 7 |
Sequence similaritiesi
Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
GeneTreei | ENSGT00550000074381. |
HOGENOMi | HOG000166089. |
InParanoidi | P32798. |
KOi | K14688. |
OMAi | IFQDCAA. |
OrthoDBi | EOG092C59UM. |
Family and domain databases
Gene3Di | 1.20.1510.10. 2 hits. |
InterProi | View protein in InterPro IPR002524. Cation_efflux. IPR036837. Cation_efflux_CTD_sf. IPR027469. Cation_efflux_TMD_sf. |
Pfami | View protein in Pfam PF01545. Cation_efflux. 1 hit. |
SUPFAMi | SSF160240. SSF160240. 1 hit. SSF161111. SSF161111. 2 hits. |
TIGRFAMsi | TIGR01297. CDF. 1 hit. |
i Sequence
Sequence statusi: Complete.
P32798-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKLGSKQVKI ISLLLLDTVF FGIEITTGYL SHSLALIADS FHMLNDIISL
60 70 80 90 100
VVALWAVNVA KNRNPDSTYT YGWKRAEILG ALINAVFLIA LCVSILIEAL
110 120 130 140 150
QRIIAPPVIE NPKFVLYVGV AGLISNTVGL FLFHDNDQEH GHGHGHSHGG
160 170 180 190 200
IFADHEMHMP SSHTHTHAHV DGIENTTPMD STDNISEIMP NAIVDSFMNE
210 220 230 240 250
NTRLLTPENA SKTPSYSTSS HTIASGGNYT EHNKRKRSLN MHGVFLHVLG
260 270 280 290 300
DALGNIGVML SAFFIWKTDY SWKYYTDPLV SLIITGIIFS SALPLSCKAS
310 320 330 340 350
KILLQATPST LSGDQVEGDL LKIPGIIAIH DFHIWNLTES IFIASLHIQL
360 370 380 390 400
DISPEQFTDL AKIVRSKLHR YGIHSATLQP EFITREVTST ERAGDSQGDH
410 420 430
LQNDPLSLRP KTYGTGISGS TCLIDDAANC NTADCLEDH
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 227 | G → E in AAA74884 (PubMed:1508175).Curated | 1 | |
Sequence conflicti | 333 – 334 | HI → RV in AAA74884 (PubMed:1508175).Curated | 2 | |
Sequence conflicti | 424 | I → V in AAA74884 (PubMed:1508175).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M88252 Genomic DNA. Translation: AAA74884.1. X90565 Genomic DNA. Translation: CAA62171.1. Z75224 Genomic DNA. Translation: CAA99636.1. BK006948 Genomic DNA. Translation: DAA11080.1. |
PIRi | S58327. |
RefSeqi | NP_014961.3. NM_001183736.3. |
Genome annotation databases
EnsemblFungii | YOR316C; YOR316C; YOR316C. |
GeneIDi | 854494. |
KEGGi | sce:YOR316C. |
Similar proteinsi
Entry informationi
Entry namei | COT1_YEAST | |
Accessioni | P32798Primary (citable) accession number: P32798 Secondary accession number(s): D6W314 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | October 1, 1996 | |
Last modified: | March 28, 2018 | |
This is version 159 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |