COT1

Functionⁱ

Probably responsible for the uptake of cobalt ions. It appears to act in a dosage-dependent manner to counteract the adverse effects of cobalt ions on cells. It may participate in the regulation of cobalt levels under normal physiological conditions and may be important in the supply of metal that is required for metalloenzyme or cofactor synthesis. It reduces the toxicity of cobalt and rhodium ions. Other components responsible for cobalt transport exist.

Miscellaneous

Present with 2070 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functionⁱ

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cellular zinc ion homeostasis
Source: SGD
Inferred from mutant phenotypeⁱ
- 10856230
cobalt ion transport
Source: SGD
Inferred from mutant phenotypeⁱ
- 1508175
regulation of sequestering of zinc ion Source: GO_Central
response to zinc ion Source: GO_Central
zinc II ion transport
Source: SGD
Inferred from genetic interactionⁱ
- 8058041

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Transport
Ligand	Cobalt

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-33798-MONOMER.
Reactomeⁱ	R-SCE-264876. Insulin processing. R-SCE-425410. Metal ion SLC transporters. R-SCE-435368. Zinc efflux and compartmentalization by the SLC30 family.

Protein family/group databases

TCDBⁱ	2.A.4.2.1. the cation diffusion facilitator (cdf) family.

TCDBⁱ

2.A.4.2.1. the cation diffusion facilitator (cdf) family.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Cobalt uptake protein COT1
Gene namesⁱ	Name:COT1 Ordered Locus Names:YOR316C ORF Names:O6131
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome XV

Organism-specific databases

EuPathDBⁱ	FungiDB:YOR316C.
Saccharomyces Genome Database More...SGDⁱ	S000005843. COT1.

Topology

Feature key	Position(s)	DescriptionActions	Length
Transmembraneⁱ	10 – 27	HelicalSequence analysisAdd BLAST	18
Transmembraneⁱ	43 – 60	HelicalSequence analysisAdd BLAST	18
Transmembraneⁱ	78 – 100	HelicalSequence analysisAdd BLAST	23
Transmembraneⁱ	114 – 133	HelicalSequence analysisAdd BLAST	20
Transmembraneⁱ	244 – 265	HelicalSequence analysisAdd BLAST	22
Transmembraneⁱ	279 – 295	HelicalSequence analysisAdd BLAST	17

Keywords - Cellular componentⁱ

Membrane, Mitochondrion

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000206102	1 – 439	Cobalt uptake protein COT1Add BLAST		439

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	225	PhosphoserineCombined sources		1
Cross-linkⁱ	301	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Keywords - PTMⁱ

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P32798.
PaxDbⁱ	P32798.
PRIDEⁱ	P32798.
TopDownProteomicsⁱ	P32798.

PTM databases

iPTMnetⁱ	P32798.

iPTMnetⁱ

P32798.

Interactionⁱ

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
FTH1	P38310	3	EBI-5006,EBI-20959

With

Entry

#Exp.

IntAct

Notes

FTH1

P38310

3

EBI-5006,EBI-20959

Protein-protein interaction databases

BioGridⁱ	34703. 40 interactors.
Database of interacting proteins More...DIPⁱ	DIP-7554N.
IntActⁱ	P32798. 15 interactors.
Molecular INTeraction database More...MINTⁱ	P32798.
STRINGⁱ	4932.YOR316C.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P32798.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	140 – 148	His-rich (could be involved in coordination of cobalt ions)		9
Compositional biasⁱ	163 – 169	His-rich (could be involved in coordination of cobalt ions)		7

Sequence similaritiesⁱ

Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily. [View classification]Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00550000074381.
HOGENOMⁱ	HOG000166089.
InParanoidⁱ	P32798.
KEGG Orthology (KO) More...KOⁱ	K14688.
OMAⁱ	IFQDCAA.
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C59UM.

Family and domain databases

Gene3Dⁱ	1.20.1510.10. 2 hits.
InterProⁱ	View protein in InterPro IPR002524. Cation_efflux. IPR036837. Cation_efflux_CTD_sf. IPR027469. Cation_efflux_TMD_sf.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01545. Cation_efflux. 1 hit.
SUPFAMⁱ	SSF160240. SSF160240. 1 hit. SSF161111. SSF161111. 2 hits.
TIGRFAMsⁱ	TIGR01297. CDF. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

P32798-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKLGSKQVKI ISLLLLDTVF FGIEITTGYL SHSLALIADS FHMLNDIISL 
        60         70         80         90        100
VVALWAVNVA KNRNPDSTYT YGWKRAEILG ALINAVFLIA LCVSILIEAL 
       110        120        130        140        150
QRIIAPPVIE NPKFVLYVGV AGLISNTVGL FLFHDNDQEH GHGHGHSHGG 
       160        170        180        190        200
IFADHEMHMP SSHTHTHAHV DGIENTTPMD STDNISEIMP NAIVDSFMNE 
       210        220        230        240        250
NTRLLTPENA SKTPSYSTSS HTIASGGNYT EHNKRKRSLN MHGVFLHVLG 
       260        270        280        290        300
DALGNIGVML SAFFIWKTDY SWKYYTDPLV SLIITGIIFS SALPLSCKAS 
       310        320        330        340        350
KILLQATPST LSGDQVEGDL LKIPGIIAIH DFHIWNLTES IFIASLHIQL 
       360        370        380        390        400
DISPEQFTDL AKIVRSKLHR YGIHSATLQP EFITREVTST ERAGDSQGDH 
       410        420        430 
LQNDPLSLRP KTYGTGISGS TCLIDDAANC NTADCLEDH

Length:439

Mass (Da):48,155

Last modified:October 1, 1996 - v2

Checksum:ⁱAC88AAA5F2EE4AED

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	227	G → E in AAA74884 (PubMed:1508175).Curated	1
Sequence conflictⁱ	333 – 334	HI → RV in AAA74884 (PubMed:1508175).Curated	2
Sequence conflictⁱ	424	I → V in AAA74884 (PubMed:1508175).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M88252 Genomic DNA. Translation: AAA74884.1. X90565 Genomic DNA. Translation: CAA62171.1. Z75224 Genomic DNA. Translation: CAA99636.1. BK006948 Genomic DNA. Translation: DAA11080.1.
PIRⁱ	S58327.
NCBI Reference Sequences More...RefSeqⁱ	NP_014961.3. NM_001183736.3.

Genome annotation databases

EnsemblFungiⁱ	YOR316C; YOR316C; YOR316C.
GeneIDⁱ	854494.
KEGGⁱ	sce:YOR316C.

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P32798	A0A0L8VGU6 N1NXG6 B5VSJ1 E7NMT5 E7Q9I8	Saccharomyces sp. 'boulardii' Saccharomyces cerevisiae (strain CEN.PK113-7D) (Baker's yeast) Saccharomyces cerevisiae (strain AWRI1631) (Baker's yeast) Saccharomyces cerevisiae (strain FostersO) (Baker's yeast) Saccharomyces cerevisiae (strain FostersB) (Baker's yeast)	439	UniRef100_P32798	Cluster: Cobalt uptake protein COT1	6

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P32798	A0A0L8VGU6 N1NXG6 B5VSJ1 E7NMT5 E7Q9I8 C7GNU7 G2WNI2 A6ZPF0 H0GP16 E7M0S2 +1	Saccharomyces sp. 'boulardii' Saccharomyces cerevisiae (strain CEN.PK113-7D) (Baker's yeast) Saccharomyces cerevisiae (strain AWRI1631) (Baker's yeast) Saccharomyces cerevisiae (strain FostersO) (Baker's yeast) Saccharomyces cerevisiae (strain FostersB) (Baker's yeast) Saccharomyces cerevisiae (strain JAY291) (Baker's yeast) Saccharomyces cerevisiae (strain Kyokai no. 7 / NBRC 101557) (Baker's yeast) Saccharomyces cerevisiae (strain YJM789) (Baker's yeast) Saccharomyces cerevisiae x Saccharomyces kudriavzevii (strain VIN7) (Yeast) Saccharomyces cerevisiae (strain VIN 13) (Baker's yeast) And more	439	UniRef90_P32798	Cluster: Cobalt uptake protein COT1	12

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P32798	A0A0L8VGU6 N1NXG6 B5VSJ1 E7NMT5 E7Q9I8 E7M0S2 C7GNU7 G2WNI2 A6ZPF0 H0GP16 +13	Saccharomyces sp. 'boulardii' Saccharomyces cerevisiae (strain CEN.PK113-7D) (Baker's yeast) Saccharomyces cerevisiae (strain AWRI1631) (Baker's yeast) Saccharomyces cerevisiae (strain FostersO) (Baker's yeast) Saccharomyces cerevisiae (strain FostersB) (Baker's yeast) Saccharomyces cerevisiae (strain VIN 13) (Baker's yeast) Saccharomyces cerevisiae (strain JAY291) (Baker's yeast) Saccharomyces cerevisiae (strain Kyokai no. 7 / NBRC 101557) (Baker's yeast) Saccharomyces cerevisiae (strain YJM789) (Baker's yeast) Saccharomyces cerevisiae x Saccharomyces kudriavzevii (strain VIN7) (Yeast) And more	439	UniRef50_P32798	Cluster: Cobalt uptake protein COT1	24

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M88252 Genomic DNA. Translation: AAA74884.1. X90565 Genomic DNA. Translation: CAA62171.1. Z75224 Genomic DNA. Translation: CAA99636.1. BK006948 Genomic DNA. Translation: DAA11080.1.
PIRⁱ	S58327.
NCBI Reference Sequences More...RefSeqⁱ	NP_014961.3. NM_001183736.3.

3D structure databases

ProteinModelPortalⁱ	P32798.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	34703. 40 interactors.
Database of interacting proteins More...DIPⁱ	DIP-7554N.
IntActⁱ	P32798. 15 interactors.
Molecular INTeraction database More...MINTⁱ	P32798.
STRINGⁱ	4932.YOR316C.

Protein family/group databases

TCDBⁱ	2.A.4.2.1. the cation diffusion facilitator (cdf) family.

TCDBⁱ

2.A.4.2.1. the cation diffusion facilitator (cdf) family.

PTM databases

iPTMnetⁱ	P32798.

iPTMnetⁱ

P32798.

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P32798.
PaxDbⁱ	P32798.
PRIDEⁱ	P32798.
TopDownProteomicsⁱ	P32798.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	YOR316C; YOR316C; YOR316C.
GeneIDⁱ	854494.
KEGGⁱ	sce:YOR316C.

Organism-specific databases

EuPathDBⁱ	FungiDB:YOR316C.
Saccharomyces Genome Database More...SGDⁱ	S000005843. COT1.

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00550000074381.
HOGENOMⁱ	HOG000166089.
InParanoidⁱ	P32798.
KEGG Orthology (KO) More...KOⁱ	K14688.
OMAⁱ	IFQDCAA.
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C59UM.

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-33798-MONOMER.
Reactomeⁱ	R-SCE-264876. Insulin processing. R-SCE-425410. Metal ion SLC transporters. R-SCE-435368. Zinc efflux and compartmentalization by the SLC30 family.

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P32798.

PROⁱ

PR:P32798.

Family and domain databases

Gene3Dⁱ	1.20.1510.10. 2 hits.
InterProⁱ	View protein in InterPro IPR002524. Cation_efflux. IPR036837. Cation_efflux_CTD_sf. IPR027469. Cation_efflux_TMD_sf.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01545. Cation_efflux. 1 hit.
SUPFAMⁱ	SSF160240. SSF160240. 1 hit. SSF161111. SSF161111. 2 hits.
TIGRFAMsⁱ	TIGR01297. CDF. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	COT1_YEAST
Accessionⁱ	P32798Primary (citable) accession number: P32798 Secondary accession number(s): D6W314
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
	Last sequence update:	October 1, 1996
	Last modified:	March 28, 2018
	This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P32798 (COT1_YEAST)

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Cobalt uptake protein COT1

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Mitochondrion

Mitochondrion

Vacuole

Other locations

Topology

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ