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Protein

Cobalt uptake protein COT1

Gene

COT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probably responsible for the uptake of cobalt ions. It appears to act in a dosage-dependent manner to counteract the adverse effects of cobalt ions on cells. It may participate in the regulation of cobalt levels under normal physiological conditions and may be important in the supply of metal that is required for metalloenzyme or cofactor synthesis. It reduces the toxicity of cobalt and rhodium ions. Other components responsible for cobalt transport exist.

GO - Molecular functioni

  • cobalt ion transmembrane transporter activity Source: SGD
  • zinc ion transmembrane transporter activity Source: SGD

GO - Biological processi

  • cellular zinc ion homeostasis Source: SGD
  • cobalt ion transport Source: SGD
  • zinc II ion transmembrane transport Source: GOC
  • zinc II ion transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

Cobalt

Enzyme and pathway databases

BioCyciYEAST:G3O-33798-MONOMER.
ReactomeiR-SCE-264876. Insulin processing.
R-SCE-435368. Zinc efflux and compartmentalization by the SLC30 family.

Protein family/group databases

TCDBi2.A.4.2.1. the cation diffusion facilitator (cdf) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Cobalt uptake protein COT1
Gene namesi
Name:COT1
Ordered Locus Names:YOR316C
ORF Names:O6131
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR316C.
SGDiS000005843. COT1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei10 – 2718HelicalSequence analysisAdd
BLAST
Transmembranei43 – 6018HelicalSequence analysisAdd
BLAST
Transmembranei78 – 10023HelicalSequence analysisAdd
BLAST
Transmembranei114 – 13320HelicalSequence analysisAdd
BLAST
Transmembranei244 – 26522HelicalSequence analysisAdd
BLAST
Transmembranei279 – 29517HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • fungal-type vacuole Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 439439Cobalt uptake protein COT1PRO_0000206102Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei225 – 2251PhosphoserineCombined sources
Cross-linki301 – 301Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP32798.
TopDownProteomicsiP32798.

PTM databases

iPTMnetiP32798.

Interactioni

Protein-protein interaction databases

BioGridi34703. 23 interactions.
DIPiDIP-7554N.
IntActiP32798. 13 interactions.
MINTiMINT-4503876.

Structurei

3D structure databases

ProteinModelPortaliP32798.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi140 – 1489His-rich (could be involved in coordination of cobalt ions)
Compositional biasi163 – 1697His-rich (could be involved in coordination of cobalt ions)

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000074381.
HOGENOMiHOG000166089.
InParanoidiP32798.
KOiK14688.
OMAiVIAYISV.
OrthoDBiEOG7C5MJX.

Family and domain databases

Gene3Di1.20.1510.10. 2 hits.
InterProiIPR002524. Cation_efflux.
IPR027469. Cation_efflux_TMD.
[Graphical view]
PANTHERiPTHR11562. PTHR11562. 2 hits.
PfamiPF01545. Cation_efflux. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01297. CDF. 1 hit.

Sequencei

Sequence statusi: Complete.

P32798-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLGSKQVKI ISLLLLDTVF FGIEITTGYL SHSLALIADS FHMLNDIISL
60 70 80 90 100
VVALWAVNVA KNRNPDSTYT YGWKRAEILG ALINAVFLIA LCVSILIEAL
110 120 130 140 150
QRIIAPPVIE NPKFVLYVGV AGLISNTVGL FLFHDNDQEH GHGHGHSHGG
160 170 180 190 200
IFADHEMHMP SSHTHTHAHV DGIENTTPMD STDNISEIMP NAIVDSFMNE
210 220 230 240 250
NTRLLTPENA SKTPSYSTSS HTIASGGNYT EHNKRKRSLN MHGVFLHVLG
260 270 280 290 300
DALGNIGVML SAFFIWKTDY SWKYYTDPLV SLIITGIIFS SALPLSCKAS
310 320 330 340 350
KILLQATPST LSGDQVEGDL LKIPGIIAIH DFHIWNLTES IFIASLHIQL
360 370 380 390 400
DISPEQFTDL AKIVRSKLHR YGIHSATLQP EFITREVTST ERAGDSQGDH
410 420 430
LQNDPLSLRP KTYGTGISGS TCLIDDAANC NTADCLEDH
Length:439
Mass (Da):48,155
Last modified:October 1, 1996 - v2
Checksum:iAC88AAA5F2EE4AED
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti227 – 2271G → E in AAA74884 (PubMed:1508175).Curated
Sequence conflicti333 – 3342HI → RV in AAA74884 (PubMed:1508175).Curated
Sequence conflicti424 – 4241I → V in AAA74884 (PubMed:1508175).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88252 Genomic DNA. Translation: AAA74884.1.
X90565 Genomic DNA. Translation: CAA62171.1.
Z75224 Genomic DNA. Translation: CAA99636.1.
BK006948 Genomic DNA. Translation: DAA11080.1.
PIRiS58327.
RefSeqiNP_014961.3. NM_001183736.3.

Genome annotation databases

EnsemblFungiiYOR316C; YOR316C; YOR316C.
GeneIDi854494.
KEGGisce:YOR316C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88252 Genomic DNA. Translation: AAA74884.1.
X90565 Genomic DNA. Translation: CAA62171.1.
Z75224 Genomic DNA. Translation: CAA99636.1.
BK006948 Genomic DNA. Translation: DAA11080.1.
PIRiS58327.
RefSeqiNP_014961.3. NM_001183736.3.

3D structure databases

ProteinModelPortaliP32798.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34703. 23 interactions.
DIPiDIP-7554N.
IntActiP32798. 13 interactions.
MINTiMINT-4503876.

Protein family/group databases

TCDBi2.A.4.2.1. the cation diffusion facilitator (cdf) family.

PTM databases

iPTMnetiP32798.

Proteomic databases

MaxQBiP32798.
TopDownProteomicsiP32798.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR316C; YOR316C; YOR316C.
GeneIDi854494.
KEGGisce:YOR316C.

Organism-specific databases

EuPathDBiFungiDB:YOR316C.
SGDiS000005843. COT1.

Phylogenomic databases

GeneTreeiENSGT00550000074381.
HOGENOMiHOG000166089.
InParanoidiP32798.
KOiK14688.
OMAiVIAYISV.
OrthoDBiEOG7C5MJX.

Enzyme and pathway databases

BioCyciYEAST:G3O-33798-MONOMER.
ReactomeiR-SCE-264876. Insulin processing.
R-SCE-435368. Zinc efflux and compartmentalization by the SLC30 family.

Miscellaneous databases

PROiP32798.

Family and domain databases

Gene3Di1.20.1510.10. 2 hits.
InterProiIPR002524. Cation_efflux.
IPR027469. Cation_efflux_TMD.
[Graphical view]
PANTHERiPTHR11562. PTHR11562. 2 hits.
PfamiPF01545. Cation_efflux. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01297. CDF. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "COT1, a gene involved in cobalt accumulation in Saccharomyces cerevisiae."
    Conklin D.S., McMaster J.A., Culbertson M.R., Kung C.
    Mol. Cell. Biol. 12:3678-3688(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequencing of a 35.71 kb DNA segment on the right arm of yeast chromosome XV reveals regions of similarity to chromosomes I and XIII."
    Pearson B.M., Hernando Y., Payne J., Wolf S.S., Kalogeropoulos A., Schweizer M.
    Yeast 12:1021-1031(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery."
    Hitchcock A.L., Auld K., Gygi S.P., Silver P.A.
    Proc. Natl. Acad. Sci. U.S.A. 100:12735-12740(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-301.
  7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCOT1_YEAST
AccessioniPrimary (citable) accession number: P32798
Secondary accession number(s): D6W314
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2070 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.