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P32798 (COT1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cobalt uptake protein COT1
Gene names
Name:COT1
Ordered Locus Names:YOR316C
ORF Names:O6131
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length439 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probably responsible for the uptake of cobalt ions. It appears to act in a dosage-dependent manner to counteract the adverse effects of cobalt ions on cells. It may participate in the regulation of cobalt levels under normal physiological conditions and may be important in the supply of metal that is required for metalloenzyme or cofactor synthesis. It reduces the toxicity of cobalt and rhodium ions. Other components responsible for cobalt transport exist.

Subcellular location

Mitochondrion membrane; Multi-pass membrane protein. Note: Another possibility exists that it is associated with another unidentified membrane that has been enriched in the mitochondrial membrane fractions.

Miscellaneous

Present with 2070 molecules/cell in log phase SD medium. Ref.5

Sequence similarities

Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 439439Cobalt uptake protein COT1
PRO_0000206102

Regions

Transmembrane10 – 2718Helical; Potential
Transmembrane43 – 6018Helical; Potential
Transmembrane78 – 10023Helical; Potential
Transmembrane114 – 13320Helical; Potential
Transmembrane244 – 26522Helical; Potential
Transmembrane279 – 29517Helical; Potential
Compositional bias140 – 1489His-rich (could be involved in coordination of cobalt ions)
Compositional bias163 – 1697His-rich (could be involved in coordination of cobalt ions)

Amino acid modifications

Modified residue2061Phosphothreonine Ref.8
Modified residue2251Phosphoserine Ref.7
Cross-link301Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.6

Experimental info

Sequence conflict2271G → E in AAA74884. Ref.1
Sequence conflict333 – 3342HI → RV in AAA74884. Ref.1
Sequence conflict4241I → V in AAA74884. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P32798 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: AC88AAA5F2EE4AED

FASTA43948,155
        10         20         30         40         50         60 
MKLGSKQVKI ISLLLLDTVF FGIEITTGYL SHSLALIADS FHMLNDIISL VVALWAVNVA 

        70         80         90        100        110        120 
KNRNPDSTYT YGWKRAEILG ALINAVFLIA LCVSILIEAL QRIIAPPVIE NPKFVLYVGV 

       130        140        150        160        170        180 
AGLISNTVGL FLFHDNDQEH GHGHGHSHGG IFADHEMHMP SSHTHTHAHV DGIENTTPMD 

       190        200        210        220        230        240 
STDNISEIMP NAIVDSFMNE NTRLLTPENA SKTPSYSTSS HTIASGGNYT EHNKRKRSLN 

       250        260        270        280        290        300 
MHGVFLHVLG DALGNIGVML SAFFIWKTDY SWKYYTDPLV SLIITGIIFS SALPLSCKAS 

       310        320        330        340        350        360 
KILLQATPST LSGDQVEGDL LKIPGIIAIH DFHIWNLTES IFIASLHIQL DISPEQFTDL 

       370        380        390        400        410        420 
AKIVRSKLHR YGIHSATLQP EFITREVTST ERAGDSQGDH LQNDPLSLRP KTYGTGISGS 

       430 
TCLIDDAANC NTADCLEDH

« Hide

References

« Hide 'large scale' references
[1]"COT1, a gene involved in cobalt accumulation in Saccharomyces cerevisiae."
Conklin D.S., McMaster J.A., Culbertson M.R., Kung C.
Mol. Cell. Biol. 12:3678-3688(1992) [PubMed: 1508175] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequencing of a 35.71 kb DNA segment on the right arm of yeast chromosome XV reveals regions of similarity to chromosomes I and XIII."
Pearson B.M., Hernando Y., Payne J., Wolf S.S., Kalogeropoulos A., Schweizer M.
Yeast 12:1021-1031(1996) [PubMed: 8896266] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed: 9169874] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery."
Hitchcock A.L., Auld K., Gygi S.P., Silver P.A.
Proc. Natl. Acad. Sci. U.S.A. 100:12735-12740(2003) [PubMed: 14557538] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-301, MASS SPECTROMETRY.
[7]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225, MASS SPECTROMETRY.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-206, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M88252 Genomic DNA. Translation: AAA74884.1.
X90565 Genomic DNA. Translation: CAA62171.1.
Z75224 Genomic DNA. Translation: CAA99636.1.
BK006948 Genomic DNA. Translation: DAA11080.1.
PIRS58327.
RefSeqNP_014961.1. NM_001183736.1.

3D structure databases

ProteinModelPortalP32798.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-7554N.
IntActP32798. 13 interactions.
MINTMINT-4503876.
STRINGP32798.

Protein family/group databases

TCDB2.A.4.2.1. cation diffusion facilitator (CDF) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOR316C; YOR316C; YOR316C.
GeneID854494.
KEGGsce:YOR316C.
NMPDRfig|4932.3.peg.6075.

Organism-specific databases

CYGDYOR316c.
SGDS000005843. COT1.

Phylogenomic databases

eggNOGfuNOG05089.
GeneTreeEFGT00050000001555.
HOGENOMHBG317426.
OMAKKEKHGH.
OrthoDBEOG4XSPZX.

Gene expression databases

ArrayExpressP32798.
GenevestigatorP32798.
GermOnlineYOR316C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR002524. Cation_efflux.
[Graphical view]
KOK14688.
PANTHERPTHR11562. Cation_efflux. 1 hit.
PfamPF01545. Cation_efflux. 1 hit.
[Graphical view]
TIGRFAMsTIGR01297. CDF. 1 hit.
ProtoNetSearch...

Other

NextBio976825.

Entry information

Entry nameCOT1_YEAST
AccessionPrimary (citable) accession number: P32798
Secondary accession number(s): D6W314
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1996
Last modified: December 14, 2011
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents