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Protein

Carnitine O-acetyltransferase, mitochondrial

Gene

CAT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Carnitine acetylase is specific for short chain fatty acids. Carnitine acetylase seems to affect the flux through the pyruvate dehydrogenase complex. It may be involved as well in the transport of acetyl-CoA into mitochondria.

Catalytic activityi

Acetyl-CoA + carnitine = CoA + O-acetylcarnitine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei378 – 3781Proton acceptorBy similarity
Binding sitei461 – 4611Coenzyme ABy similarity
Binding sitei494 – 4941CarnitineBy similarity
Binding sitei498 – 4981Coenzyme A; via amide nitrogenBy similarity
Binding sitei507 – 5071CarnitineBy similarity
Binding sitei597 – 5971Coenzyme A; via carbonyl oxygenBy similarity

GO - Molecular functioni

  • carnitine O-acetyltransferase activity Source: SGD

GO - Biological processi

  • carnitine metabolic process Source: SGD
  • fatty acid metabolic process Source: UniProtKB-KW
  • transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism, Transport

Enzyme and pathway databases

BioCyciYEAST:YML042W-MONOMER.
BRENDAi2.3.1.7. 984.
2.3.1.8. 984.
ReactomeiR-SCE-1483191. Synthesis of PC.
R-SCE-200425. Import of palmitoyl-CoA into the mitochondrial matrix.
R-SCE-264642. Acetylcholine Neurotransmitter Release Cycle.
R-SCE-389887. Beta-oxidation of pristanoyl-CoA.
R-SCE-390247. Beta-oxidation of very long chain fatty acids.
R-SCE-5362517. Signaling by Retinoic Acid.

Names & Taxonomyi

Protein namesi
Recommended name:
Carnitine O-acetyltransferase, mitochondrial (EC:2.3.1.7)
Short name:
Carnitine acetylase
Gene namesi
Name:CAT2
Synonyms:CAT, YCAT
Ordered Locus Names:YML042W
ORF Names:YM8054.01
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML042W.
SGDiS000004506. CAT2.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial inner membrane Source: UniProtKB-SubCell
  • mitochondrion Source: SGD
  • peroxisome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 670Carnitine O-acetyltransferase, mitochondrialPRO_0000004431
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

MaxQBiP32796.

Expressioni

Inductioni

By ethanol. Repressed by galactose.

Interactioni

Protein-protein interaction databases

BioGridi35127. 33 interactions.
DIPiDIP-2476N.
IntActiP32796. 10 interactions.
MINTiMINT-492532.

Structurei

3D structure databases

ProteinModelPortaliP32796.
SMRiP32796. Positions 67-663.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni465 – 4728Coenzyme A bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi668 – 6703Microbody targeting signalSequence analysis

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00760000119220.
HOGENOMiHOG000233845.
InParanoidiP32796.
KOiK00624.
OMAiRDQWREV.
OrthoDBiEOG7NCVC3.

Family and domain databases

InterProiIPR000542. Carn_acyl_trans.
[Graphical view]
PANTHERiPTHR22589. PTHR22589. 1 hit.
PfamiPF00755. Carn_acyltransf. 1 hit.
[Graphical view]
PROSITEiPS00439. ACYLTRANSF_C_1. 1 hit.
PS00440. ACYLTRANSF_C_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Mitochondrial (identifier: P32796-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRICHSRTLS NLKDLPITSR RAMHSAIVNY STQKAQFPVE TNNGEHYWAE
60 70 80 90 100
KPNKFYQNKR PNFQGITFAK QQDLPSLPVP ELKSTLDKYL QTIRPFCNDV
110 120 130 140 150
ETFERQQLLC KDFSEHMGPI LQDRLKEYAN DKRNWMAKFW DEQSYLQYND
160 170 180 190 200
PIVPYVSYFY SHMPLPNHLS KIDNDPLIKA TAIISTVVKF IEAIKDESLP
210 220 230 240 250
VEIIKGMPFC MNSFSLMFNT SRLPGKPEDN QDTNIFYSVY ENNFVTIAYK
260 270 280 290 300
GKFYKLMTHD GNDKPLSENE IWRQLYSVVF QGSQSDPKLG GIGSLTSLPR
310 320 330 340 350
DQWREVHLEL MKDPISQDSL ETIHKSSFML CLDLDQSPVT LEEKSRNCWH
360 370 380 390 400
GDGINRFYDK SLQFLVTGNG SSGFLAEHSK MDGTPTLFLN NYVCQQLNKL
410 420 430 440 450
DVDDFMRKVI TPSSTVAMKP MELPFIITPK IHKAIESAQL QFKETIGEHD
460 470 480 490 500
LRVWHYNKYG KTFIKRHGMS PDAFIQQVIQ LAVFKYLKRQ LPTYEAASTR
510 520 530 540 550
KYFKGRTETG RSVSTASLEF VSKWQNGDVP IAEKIQALKH SAKEHSTYLK
560 570 580 590 600
NAANGNGVDR HFFGLKNMLK SNDDQIPPLF KDPLFNYSST WLISTSQLSS
610 620 630 640 650
EYFDGYGWSQ VNDNGFGLAY MLNNEWLHIN IVNKPAKSGA SVNRLHYYLS
660 670
QAADEIFDAL ENENKRKAKL
Length:670
Mass (Da):77,242
Last modified:February 1, 1996 - v2
Checksum:iC707BAC7ECB4D095
GO
Isoform Peroxisomal (identifier: P32796-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: Missing.

Note: Produced by alternative initiation at Met-23 of isoform Mitochondrial.
Show »
Length:648
Mass (Da):74,691
Checksum:i36FED5BA0F5C6BA1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti263 – 2631D → H in CAA78399 (PubMed:8420957).Curated
Sequence conflicti308 – 3081L → M in CAA78399 (PubMed:8420957).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2222Missing in isoform Peroxisomal. CuratedVSP_018690Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14021 Genomic DNA. Translation: CAA78399.1.
Z48430 Genomic DNA. Translation: CAA88327.1.
BK006946 Genomic DNA. Translation: DAA09857.1.
PIRiS52478.
RefSeqiNP_013670.1. NM_001182400.1. [P32796-1]

Genome annotation databases

EnsemblFungiiYML042W; YML042W; YML042W. [P32796-1]
GeneIDi854965.
KEGGisce:YML042W.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14021 Genomic DNA. Translation: CAA78399.1.
Z48430 Genomic DNA. Translation: CAA88327.1.
BK006946 Genomic DNA. Translation: DAA09857.1.
PIRiS52478.
RefSeqiNP_013670.1. NM_001182400.1. [P32796-1]

3D structure databases

ProteinModelPortaliP32796.
SMRiP32796. Positions 67-663.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35127. 33 interactions.
DIPiDIP-2476N.
IntActiP32796. 10 interactions.
MINTiMINT-492532.

Proteomic databases

MaxQBiP32796.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML042W; YML042W; YML042W. [P32796-1]
GeneIDi854965.
KEGGisce:YML042W.

Organism-specific databases

EuPathDBiFungiDB:YML042W.
SGDiS000004506. CAT2.

Phylogenomic databases

GeneTreeiENSGT00760000119220.
HOGENOMiHOG000233845.
InParanoidiP32796.
KOiK00624.
OMAiRDQWREV.
OrthoDBiEOG7NCVC3.

Enzyme and pathway databases

BioCyciYEAST:YML042W-MONOMER.
BRENDAi2.3.1.7. 984.
2.3.1.8. 984.
ReactomeiR-SCE-1483191. Synthesis of PC.
R-SCE-200425. Import of palmitoyl-CoA into the mitochondrial matrix.
R-SCE-264642. Acetylcholine Neurotransmitter Release Cycle.
R-SCE-389887. Beta-oxidation of pristanoyl-CoA.
R-SCE-390247. Beta-oxidation of very long chain fatty acids.
R-SCE-5362517. Signaling by Retinoic Acid.

Miscellaneous databases

PROiP32796.

Family and domain databases

InterProiIPR000542. Carn_acyl_trans.
[Graphical view]
PANTHERiPTHR22589. PTHR22589. 1 hit.
PfamiPF00755. Carn_acyltransf. 1 hit.
[Graphical view]
PROSITEiPS00439. ACYLTRANSF_C_1. 1 hit.
PS00440. ACYLTRANSF_C_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of a cDNA encoding Saccharomyces cerevisiae carnitine acetyltransferase. Use of the cDNA in gene disruption studies."
    Kispal G., Sumegi B., Dietmeier K., Bock I., Gajdos G., Tomcsanyi T., Sandor A.
    J. Biol. Chem. 268:1824-1829(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Peroxisomal and mitochondrial carnitine acetyltransferases of Saccharomyces cerevisiae are encoded by a single gene."
    Elgersma Y., van Roermund C.W.T., Wanders R.J.A., Tabak H.F.
    EMBO J. 14:3472-3479(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE INITIATION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCACP_YEAST
AccessioniPrimary (citable) accession number: P32796
Secondary accession number(s): D6VZD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1996
Last modified: July 6, 2016
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 450 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.