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Protein

Carnitine O-acetyltransferase, mitochondrial

Gene

CAT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Carnitine acetylase is specific for short chain fatty acids. Carnitine acetylase seems to affect the flux through the pyruvate dehydrogenase complex. It may be involved as well in the transport of acetyl-CoA into mitochondria.

Miscellaneous

Present with 450 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Acetyl-CoA + carnitine = CoA + O-acetylcarnitine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei378Proton acceptorBy similarity1
Binding sitei461Coenzyme ABy similarity1
Binding sitei494CarnitineBy similarity1
Binding sitei498Coenzyme A; via amide nitrogenBy similarity1
Binding sitei507CarnitineBy similarity1
Binding sitei597Coenzyme A; via carbonyl oxygenBy similarity1

GO - Molecular functioni

  • carnitine O-acetyltransferase activity Source: SGD

GO - Biological processi

  • carnitine metabolic process Source: SGD
  • fatty acid metabolic process Source: UniProtKB-KW

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processFatty acid metabolism, Lipid metabolism, Transport

Enzyme and pathway databases

BioCyciYEAST:YML042W-MONOMER
BRENDAi2.3.1.7 984
2.3.1.8 984
ReactomeiR-SCE-1483191 Synthesis of PC
R-SCE-200425 Import of palmitoyl-CoA into the mitochondrial matrix
R-SCE-264642 Acetylcholine Neurotransmitter Release Cycle
R-SCE-389887 Beta-oxidation of pristanoyl-CoA
R-SCE-5362517 Signaling by Retinoic Acid
R-SCE-9033241 Peroxisomal protein import

Names & Taxonomyi

Protein namesi
Recommended name:
Carnitine O-acetyltransferase, mitochondrial (EC:2.3.1.7)
Short name:
Carnitine acetylase
Gene namesi
Name:CAT2
Synonyms:CAT, YCAT
Ordered Locus Names:YML042W
ORF Names:YM8054.01
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML042W
SGDiS000004506 CAT2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000004431? – 670Carnitine O-acetyltransferase, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

PaxDbiP32796
PRIDEiP32796

Expressioni

Inductioni

By ethanol. Repressed by galactose.

Interactioni

Protein-protein interaction databases

BioGridi35127, 58 interactors
DIPiDIP-2476N
IntActiP32796, 9 interactors
STRINGi4932.YML042W

Structurei

3D structure databases

ProteinModelPortaliP32796
SMRiP32796
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni465 – 472Coenzyme A bindingBy similarity8

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi668 – 670Microbody targeting signalSequence analysis3

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00760000119220
HOGENOMiHOG000233845
InParanoidiP32796
KOiK00624
OMAiWWNDAAY
OrthoDBiEOG092C0RQ8

Family and domain databases

InterProiView protein in InterPro
IPR000542 Carn_acyl_trans
PANTHERiPTHR22589 PTHR22589, 1 hit
PfamiView protein in Pfam
PF00755 Carn_acyltransf, 1 hit
PROSITEiView protein in PROSITE
PS00439 ACYLTRANSF_C_1, 1 hit
PS00440 ACYLTRANSF_C_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Mitochondrial (identifier: P32796-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRICHSRTLS NLKDLPITSR RAMHSAIVNY STQKAQFPVE TNNGEHYWAE
60 70 80 90 100
KPNKFYQNKR PNFQGITFAK QQDLPSLPVP ELKSTLDKYL QTIRPFCNDV
110 120 130 140 150
ETFERQQLLC KDFSEHMGPI LQDRLKEYAN DKRNWMAKFW DEQSYLQYND
160 170 180 190 200
PIVPYVSYFY SHMPLPNHLS KIDNDPLIKA TAIISTVVKF IEAIKDESLP
210 220 230 240 250
VEIIKGMPFC MNSFSLMFNT SRLPGKPEDN QDTNIFYSVY ENNFVTIAYK
260 270 280 290 300
GKFYKLMTHD GNDKPLSENE IWRQLYSVVF QGSQSDPKLG GIGSLTSLPR
310 320 330 340 350
DQWREVHLEL MKDPISQDSL ETIHKSSFML CLDLDQSPVT LEEKSRNCWH
360 370 380 390 400
GDGINRFYDK SLQFLVTGNG SSGFLAEHSK MDGTPTLFLN NYVCQQLNKL
410 420 430 440 450
DVDDFMRKVI TPSSTVAMKP MELPFIITPK IHKAIESAQL QFKETIGEHD
460 470 480 490 500
LRVWHYNKYG KTFIKRHGMS PDAFIQQVIQ LAVFKYLKRQ LPTYEAASTR
510 520 530 540 550
KYFKGRTETG RSVSTASLEF VSKWQNGDVP IAEKIQALKH SAKEHSTYLK
560 570 580 590 600
NAANGNGVDR HFFGLKNMLK SNDDQIPPLF KDPLFNYSST WLISTSQLSS
610 620 630 640 650
EYFDGYGWSQ VNDNGFGLAY MLNNEWLHIN IVNKPAKSGA SVNRLHYYLS
660 670
QAADEIFDAL ENENKRKAKL
Length:670
Mass (Da):77,242
Last modified:February 1, 1996 - v2
Checksum:iC707BAC7ECB4D095
GO
Isoform Peroxisomal (identifier: P32796-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: Missing.

Note: Produced by alternative initiation at Met-23 of isoform Mitochondrial.
Show »
Length:648
Mass (Da):74,691
Checksum:i36FED5BA0F5C6BA1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti263D → H in CAA78399 (PubMed:8420957).Curated1
Sequence conflicti308L → M in CAA78399 (PubMed:8420957).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0186901 – 22Missing in isoform Peroxisomal. CuratedAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14021 Genomic DNA Translation: CAA78399.1
Z48430 Genomic DNA Translation: CAA88327.1
BK006946 Genomic DNA Translation: DAA09857.1
PIRiS52478
RefSeqiNP_013670.1, NM_001182400.1 [P32796-1]

Genome annotation databases

EnsemblFungiiYML042W; YML042W; YML042W [P32796-1]
GeneIDi854965
KEGGisce:YML042W

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Entry informationi

Entry nameiCACP_YEAST
AccessioniPrimary (citable) accession number: P32796
Secondary accession number(s): D6VZD3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1996
Last modified: May 23, 2018
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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