P32794 (AFG2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATPase family gene 2 protein

Gene names

Name:	AFG2
Ordered Locus Names:	YLR397C
ORF Names:	L8084.16

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	780 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Not yet known. Essential for viability.
Miscellaneous	Present with 799 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the AAA ATPase family. AFG2 subfamily.

Ontologies

Keywords
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	response to drug Inferred from mutant phenotype. Source: SGD ribosomal large subunit biogenesis Inferred from mutant phenotype. Source: SGD
Cellular component	preribosome, large subunit precursor Inferred from mutant phenotype. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 780	780	ATPase family gene 2 protein		PRO_0000084592
Regions
	Nucleotide binding	286 – 293	8	ATP 1 Potential
	Nucleotide binding	557 – 564	8	ATP 2 Potential

Sequences

	Sequence		Length	Mass (Da)
	P32794 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 75094DFA3D401D4E	FASTA	780	84,748
	10 20 30 40 50 60 MAPKSSSSGS KKKSSASSNS ADAKASKFKL PAEFITRPHP SKDHGKETCT AYIHPNVLSS 70 80 90 100 110 120 LEINPGSFCT VGKIGENGIL VIARAGDEEV HPVNVITLST TIRSVGNLIL GDRLELKKAQ 130 140 150 160 170 180 VQPPYATKVT VGSLQGYNIL ECMEEKVIQK LLDDSGVIMP GMIFQNLKTK AGDESIDVVI 190 200 210 220 230 240 TDASDDSLPD VSQLDLNMDD MYGGLDNLFY LSPPFIFRKG STHITFSKET QANRKYNLPE 250 260 270 280 290 300 PLSYAAVGGL DKEIESLKSA IEIPLHQPTL FSSFGVSPPR GILLHGPPGT GKTMLLRVVA 310 320 330 340 350 360 NTSNAHVLTI NGPSIVSKYL GETEAALRDI FNEARKYQPS IIFIDEIDSI APNRANDDSG 370 380 390 400 410 420 EVESRVVATL LTLMDGMGAA GKVVVIAATN RPNSVDPALR RPGRFDQEVE IGIPDVDARF 430 440 450 460 470 480 DILTKQFSRM SSDRHVLDSE AIKYIASKTH GYVGADLTAL CRESVMKTIQ RGLGTDANID 490 500 510 520 530 540 KFSLKVTLKD VESAMVDIRP SAMREIFLEM PKVYWSDIGG QEELKTKMKE MIQLPLEASE 550 560 570 580 590 600 TFARLGISAP KGVLLYGPPG CSKTLTAKAL ATESGINFLA VKGPEIFNKY VGESERAIRE 610 620 630 640 650 660 IFRKARSAAP SIIFFDEIDA LSPDRDGSST SAANHVLTSL LNEIDGVEEL KGVVIVAATN 670 680 690 700 710 720 RPDEIDAALL RPGRLDRHIY VGPPDVNARL EILKKCTKKF NTEESGVDLH ELADRTEGYS 730 740 750 760 770 780 GAEVVLLCQE AGLAAIMEDL DVAKVELRHF EKAFKGIARG ITPEMLSYYE EFALRSGSSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"AFG2, an essential gene in yeast, encodes a new member of the Sec18p, Pas1p, Cdc48p, TBP-1 family of putative ATPases." Thorsness P.E., White K.H., Ong W.-C. Yeast 9:1267-1271(1993) [PubMed: 8109176] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L14615 Unassigned DNA. Translation: AAC37367.1. U19729 Genomic DNA. Translation: AAB82355.1. AY693116 Genomic DNA. Translation: AAT93135.1. BK006945 Genomic DNA. Translation: DAA09698.1.
PIR	S39110.
RefSeq	NP_013501.1. NM_001182285.1.
3D structure databases
ProteinModelPortal	P32794.
SMR	P32794. Positions 239-499, 518-761.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4470N.
IntAct	P32794. 9 interactions.
MINT	MINT-566202.
STRING	P32794.
Proteomic databases
PeptideAtlas	P32794.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR397C; YLR397C; YLR397C.
GeneID	851113.
KEGG	sce:YLR397C.
NMPDR	fig|4932.3.peg.4526.
Organism-specific databases
CYGD	YLR397c.
SGD	S000004389. AFG2.
Phylogenomic databases
eggNOG	fuNOG07751.
GeneTree	EFGT00050000000010.
HOGENOM	HBG597299.
OMA	GPEIFNK.
OrthoDB	EOG49CTH0.
Gene expression databases
ArrayExpress	P32794.
Genevestigator	P32794.
GermOnline	YLR397C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. [Graphical view]
KO	K14575.
Pfam	PF00004. AAA. 2 hits. [Graphical view]
SMART	SM00382. AAA. 2 hits. [Graphical view]
PROSITE	PS00674. AAA. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	967827.

Entry information

Entry name

AFG2_YEAST

Accession

Primary (citable) accession number: P32794
Secondary accession number(s): D6VZ32

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	December 14, 2011
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program