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Protein

ATPase family gene 2 protein

Gene

AFG2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Not yet known. Essential for viability.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi286 – 2938ATP 1Sequence analysis
Nucleotide bindingi557 – 5648ATP 2Sequence analysis

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: UniProtKB-KW

GO - Biological processi

  • response to drug Source: SGD
  • ribosomal large subunit biogenesis Source: SGD
Complete GO annotation...

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32461-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase family gene 2 protein
Gene namesi
Name:AFG2
Ordered Locus Names:YLR397C
ORF Names:L8084.16
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR397C.
SGDiS000004389. AFG2.

Subcellular locationi

GO - Cellular componenti

  • intracellular Source: SGD
  • preribosome, large subunit precursor Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 780780ATPase family gene 2 proteinPRO_0000084592Add
BLAST

Proteomic databases

MaxQBiP32794.
PeptideAtlasiP32794.

Interactioni

Protein-protein interaction databases

BioGridi31656. 31 interactions.
DIPiDIP-4470N.
IntActiP32794. 8 interactions.
MINTiMINT-566202.

Structurei

3D structure databases

ProteinModelPortaliP32794.
SMRiP32794. Positions 242-773.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family. AFG2 subfamily.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00810000125522.
HOGENOMiHOG000223225.
InParanoidiP32794.
KOiK14575.
OMAiEIFLEMP.
OrthoDBiEOG79CZ6Z.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00674. AAA. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32794-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPKSSSSGS KKKSSASSNS ADAKASKFKL PAEFITRPHP SKDHGKETCT
60 70 80 90 100
AYIHPNVLSS LEINPGSFCT VGKIGENGIL VIARAGDEEV HPVNVITLST
110 120 130 140 150
TIRSVGNLIL GDRLELKKAQ VQPPYATKVT VGSLQGYNIL ECMEEKVIQK
160 170 180 190 200
LLDDSGVIMP GMIFQNLKTK AGDESIDVVI TDASDDSLPD VSQLDLNMDD
210 220 230 240 250
MYGGLDNLFY LSPPFIFRKG STHITFSKET QANRKYNLPE PLSYAAVGGL
260 270 280 290 300
DKEIESLKSA IEIPLHQPTL FSSFGVSPPR GILLHGPPGT GKTMLLRVVA
310 320 330 340 350
NTSNAHVLTI NGPSIVSKYL GETEAALRDI FNEARKYQPS IIFIDEIDSI
360 370 380 390 400
APNRANDDSG EVESRVVATL LTLMDGMGAA GKVVVIAATN RPNSVDPALR
410 420 430 440 450
RPGRFDQEVE IGIPDVDARF DILTKQFSRM SSDRHVLDSE AIKYIASKTH
460 470 480 490 500
GYVGADLTAL CRESVMKTIQ RGLGTDANID KFSLKVTLKD VESAMVDIRP
510 520 530 540 550
SAMREIFLEM PKVYWSDIGG QEELKTKMKE MIQLPLEASE TFARLGISAP
560 570 580 590 600
KGVLLYGPPG CSKTLTAKAL ATESGINFLA VKGPEIFNKY VGESERAIRE
610 620 630 640 650
IFRKARSAAP SIIFFDEIDA LSPDRDGSST SAANHVLTSL LNEIDGVEEL
660 670 680 690 700
KGVVIVAATN RPDEIDAALL RPGRLDRHIY VGPPDVNARL EILKKCTKKF
710 720 730 740 750
NTEESGVDLH ELADRTEGYS GAEVVLLCQE AGLAAIMEDL DVAKVELRHF
760 770 780
EKAFKGIARG ITPEMLSYYE EFALRSGSSS
Length:780
Mass (Da):84,748
Last modified:October 1, 1993 - v1
Checksum:i75094DFA3D401D4E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14615 Unassigned DNA. Translation: AAC37367.1.
U19729 Genomic DNA. Translation: AAB82355.1.
AY693116 Genomic DNA. Translation: AAT93135.1.
BK006945 Genomic DNA. Translation: DAA09698.1.
PIRiS39110.
RefSeqiNP_013501.1. NM_001182285.1.

Genome annotation databases

EnsemblFungiiYLR397C; YLR397C; YLR397C.
GeneIDi851113.
KEGGisce:YLR397C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14615 Unassigned DNA. Translation: AAC37367.1.
U19729 Genomic DNA. Translation: AAB82355.1.
AY693116 Genomic DNA. Translation: AAT93135.1.
BK006945 Genomic DNA. Translation: DAA09698.1.
PIRiS39110.
RefSeqiNP_013501.1. NM_001182285.1.

3D structure databases

ProteinModelPortaliP32794.
SMRiP32794. Positions 242-773.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31656. 31 interactions.
DIPiDIP-4470N.
IntActiP32794. 8 interactions.
MINTiMINT-566202.

Proteomic databases

MaxQBiP32794.
PeptideAtlasiP32794.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR397C; YLR397C; YLR397C.
GeneIDi851113.
KEGGisce:YLR397C.

Organism-specific databases

EuPathDBiFungiDB:YLR397C.
SGDiS000004389. AFG2.

Phylogenomic databases

GeneTreeiENSGT00810000125522.
HOGENOMiHOG000223225.
InParanoidiP32794.
KOiK14575.
OMAiEIFLEMP.
OrthoDBiEOG79CZ6Z.

Enzyme and pathway databases

BioCyciYEAST:G3O-32461-MONOMER.

Miscellaneous databases

NextBioi967827.
PROiP32794.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00674. AAA. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "AFG2, an essential gene in yeast, encodes a new member of the Sec18p, Pas1p, Cdc48p, TBP-1 family of putative ATPases."
    Thorsness P.E., White K.H., Ong W.-C.
    Yeast 9:1267-1271(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAFG2_YEAST
AccessioniPrimary (citable) accession number: P32794
Secondary accession number(s): D6VZ32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 11, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 799 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.