Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P32793

- YSC84_YEAST

UniProt

P32793 - YSC84_YEAST

Protein

Protein YSC84

Gene

YSC84

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 2 (18 Apr 2006)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Essential for the organization of the actin cytoskeleton, fluid phase endocytosis and vesicle trafficking, together with LSB5.1 Publication

    GO - Molecular functioni

    1. actin filament binding Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. actin cortical patch localization Source: SGD
    2. actin cytoskeleton organization Source: SGD
    3. actin filament bundle assembly Source: SGD
    4. actin filament organization Source: SGD
    5. endocytosis Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31078-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein YSC84
    Alternative name(s):
    LAS seventeen-binding protein 4
    Short name:
    LAS17-binding protein 4
    Gene namesi
    Name:YSC84
    Synonyms:LSB4
    Ordered Locus Names:YHR016C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHR016c.
    SGDiS000001058. YSC84.

    Subcellular locationi

    Cytoplasmcytoskeletonactin patch 1 Publication
    Note: Cortical actin patches.

    GO - Cellular componenti

    1. actin cortical patch Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 468468Protein YSC84PRO_0000202887Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei301 – 3011Phosphoserine3 Publications
    Modified residuei311 – 3111Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP32793.
    PaxDbiP32793.
    PeptideAtlasiP32793.

    Expressioni

    Inductioni

    Induced during sporulation.1 Publication

    Gene expression databases

    GenevestigatoriP32793.

    Interactioni

    Subunit structurei

    Interacts with LAS17 and SLA1.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ABP1P158913EBI-24460,EBI-2036
    ACF2Q121687EBI-24460,EBI-32973
    ACF4P471294EBI-24460,EBI-25556
    AGP2P380902EBI-24460,EBI-2362
    AIM3P382665EBI-24460,EBI-21584
    APP1P539336EBI-24460,EBI-28798
    BSP1Q066046EBI-24460,EBI-37047
    CUE5Q084127EBI-24460,EBI-37580
    ERT1P381404EBI-24460,EBI-21048
    GTS1P409564EBI-24460,EBI-7968
    GYL1Q043226EBI-24460,EBI-27427
    GYP5Q123442EBI-24460,EBI-38508
    HUA1P403253EBI-24460,EBI-23614
    LAS17Q124467EBI-24460,EBI-10022
    NBA1Q082292EBI-24460,EBI-36841
    POM33Q121642EBI-24460,EBI-30000
    RIM101P334002EBI-24460,EBI-14422
    SEC8P328554EBI-24460,EBI-16896
    SLA1P327908EBI-24460,EBI-17313
    STP22P256042EBI-24460,EBI-411625
    YDL156WQ125102EBI-24460,EBI-35343

    Protein-protein interaction databases

    BioGridi36445. 74 interactions.
    DIPiDIP-2026N.
    IntActiP32793. 86 interactions.
    MINTiMINT-374921.
    STRINGi4932.YHR016C.

    Structurei

    Secondary structure

    1
    468
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi413 – 4186
    Beta strandi435 – 4406
    Beta strandi447 – 4537
    Beta strandi456 – 4616
    Helixi462 – 4643
    Beta strandi465 – 4673

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2A08X-ray1.54A/B413-468[»]
    ProteinModelPortaliP32793.
    SMRiP32793. Positions 413-468.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP32793.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini409 – 46860SH3PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi222 – 30786Asp-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the SH3YL1 family.Curated
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH3 domain

    Phylogenomic databases

    eggNOGiCOG2930.
    GeneTreeiENSGT00510000048137.
    HOGENOMiHOG000215564.
    OMAiERKEANS.
    OrthoDBiEOG7X9GH6.

    Family and domain databases

    InterProiIPR001452. SH3_domain.
    IPR013315. Spectrin_alpha_SH3.
    IPR007461. Ysc84_actin-binding.
    [Graphical view]
    PfamiPF04366. DUF500. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view]
    PRINTSiPR00452. SH3DOMAIN.
    PR01887. SPECTRNALPHA.
    SMARTiSM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    PROSITEiPS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32793-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGINNPIPRS LKSETKKAAK VLRSFVKPNQ VFGADQVIPP YVLKRAKGLA    50
    IITVLKAGFL FSGRAGSGVI VARLKDGTWS APSAIAMAGA GAGGMVGVEL 100
    TDFVFILNSE EAVRSFSEFG TITLGGNVSV SAGPLGRSAE AAASASTGGV 150
    SAVFAYSKSK GLFAGVSVEG SAILERREAN RKFYGDNCTS KMILSGRVKV 200
    PPAADPLLRI LESRAFNFTR HDHDDNASGD DFYDDGQYSD NTSHYYDDIP 250
    DSFDSTDESS TRPNTRSSRR RGMSLGSRSR YDDDYDDDGY GRGRGYGDFD 300
    SEDEDYDYGR SPNRNSSRNR GPQIDRGTKP RANTRWEDDL YDRDTEYSRP 350
    NHSGRDYDNT RGNRRGYGRE RGYSLGHGPT HPSNMSNVDD LSHKMSKTGL 400
    GNESTATNSA TPTAVALYNF AGEQPGDLAF KKGDVITILK KSDSQNDWWT 450
    GRTNGKEGIF PANYVRVS 468
    Length:468
    Mass (Da):50,902
    Last modified:April 18, 2006 - v2
    Checksum:iDE42E8ECF5B83C92
    GO

    Sequence cautioni

    The sequence AAA56990.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10400 Genomic DNA. Translation: AAB68945.1.
    L06795 Genomic DNA. Translation: AAA56990.1. Sequence problems.
    BK006934 Genomic DNA. Translation: DAA06705.1.
    PIRiS46791.
    RefSeqiNP_011880.1. NM_001179146.1.

    Genome annotation databases

    EnsemblFungiiYHR016C; YHR016C; YHR016C.
    GeneIDi856409.
    KEGGisce:YHR016C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10400 Genomic DNA. Translation: AAB68945.1 .
    L06795 Genomic DNA. Translation: AAA56990.1 . Sequence problems.
    BK006934 Genomic DNA. Translation: DAA06705.1 .
    PIRi S46791.
    RefSeqi NP_011880.1. NM_001179146.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2A08 X-ray 1.54 A/B 413-468 [» ]
    ProteinModelPortali P32793.
    SMRi P32793. Positions 413-468.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36445. 74 interactions.
    DIPi DIP-2026N.
    IntActi P32793. 86 interactions.
    MINTi MINT-374921.
    STRINGi 4932.YHR016C.

    Proteomic databases

    MaxQBi P32793.
    PaxDbi P32793.
    PeptideAtlasi P32793.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR016C ; YHR016C ; YHR016C .
    GeneIDi 856409.
    KEGGi sce:YHR016C.

    Organism-specific databases

    CYGDi YHR016c.
    SGDi S000001058. YSC84.

    Phylogenomic databases

    eggNOGi COG2930.
    GeneTreei ENSGT00510000048137.
    HOGENOMi HOG000215564.
    OMAi ERKEANS.
    OrthoDBi EOG7X9GH6.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31078-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P32793.
    NextBioi 981956.

    Gene expression databases

    Genevestigatori P32793.

    Family and domain databases

    InterProi IPR001452. SH3_domain.
    IPR013315. Spectrin_alpha_SH3.
    IPR007461. Ysc84_actin-binding.
    [Graphical view ]
    Pfami PF04366. DUF500. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00452. SH3DOMAIN.
    PR01887. SPECTRNALPHA.
    SMARTi SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    PROSITEi PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of two divergently transcribed genes centromere-proximal to the ARG4 locus on chromosome VIII of Saccharomyces cerevisiae."
      Rocco V., Daly M.J., Matre V., Lichten M., Nicolas A.
      Yeast 9:1111-1120(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The Saccharomyces cerevisiae homologue of human Wiskott-Aldrich syndrome protein Las17p interacts with the Arp2/3 complex."
      Madania A., Dumoulin P., Grava S., Kitamoto H., Scharer-Brodbeck C., Soulard A., Moreau V., Winsor B.
      Mol. Biol. Cell 10:3521-3538(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH LAS17.
    5. "Novel proteins linking the actin cytoskeleton to the endocytic machinery in Saccharomyces cerevisiae."
      Dewar H., Warren D.T., Gardiner F.C., Gourlay C.G., Satish N., Richardson M.R., Andrews P.D., Ayscough K.R.
      Mol. Biol. Cell 13:3646-3661(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SLA1, SUBCELLULAR LOCATION.
    6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-301, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-301 AND SER-311, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-301 AND SER-311, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiYSC84_YEAST
    AccessioniPrimary (citable) accession number: P32793
    Secondary accession number(s): D3DKW1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: April 18, 2006
    Last modified: October 1, 2014
    This is version 128 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3