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P32793

- YSC84_YEAST

UniProt

P32793 - YSC84_YEAST

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Protein
Protein YSC84
Gene
YSC84, LSB4, YHR016C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Essential for the organization of the actin cytoskeleton, fluid phase endocytosis and vesicle trafficking, together with LSB5.1 Publication

GO - Molecular functioni

  1. actin filament binding Source: SGD
  2. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. actin cortical patch localization Source: SGD
  2. actin cytoskeleton organization Source: SGD
  3. actin filament bundle assembly Source: SGD
  4. actin filament organization Source: SGD
  5. endocytosis Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-31078-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein YSC84
Alternative name(s):
LAS seventeen-binding protein 4
Short name:
LAS17-binding protein 4
Gene namesi
Name:YSC84
Synonyms:LSB4
Ordered Locus Names:YHR016C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHR016c.
SGDiS000001058. YSC84.

Subcellular locationi

Cytoplasmcytoskeletonactin patch
Note: Cortical actin patches.1 Publication

GO - Cellular componenti

  1. actin cortical patch Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 468468Protein YSC84
PRO_0000202887Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei301 – 3011Phosphoserine3 Publications
Modified residuei311 – 3111Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32793.
PaxDbiP32793.
PeptideAtlasiP32793.

Expressioni

Inductioni

Induced during sporulation.1 Publication

Gene expression databases

GenevestigatoriP32793.

Interactioni

Subunit structurei

Interacts with LAS17 and SLA1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ABP1P158913EBI-24460,EBI-2036
ACF2Q121687EBI-24460,EBI-32973
ACF4P471294EBI-24460,EBI-25556
AGP2P380902EBI-24460,EBI-2362
AIM3P382665EBI-24460,EBI-21584
APP1P539336EBI-24460,EBI-28798
BSP1Q066046EBI-24460,EBI-37047
CUE5Q084127EBI-24460,EBI-37580
ERT1P381404EBI-24460,EBI-21048
GTS1P409564EBI-24460,EBI-7968
GYL1Q043226EBI-24460,EBI-27427
GYP5Q123442EBI-24460,EBI-38508
HUA1P403253EBI-24460,EBI-23614
LAS17Q124467EBI-24460,EBI-10022
NBA1Q082292EBI-24460,EBI-36841
POM33Q121642EBI-24460,EBI-30000
RIM101P334002EBI-24460,EBI-14422
SEC8P328554EBI-24460,EBI-16896
SLA1P327908EBI-24460,EBI-17313
STP22P256042EBI-24460,EBI-411625
YDL156WQ125102EBI-24460,EBI-35343

Protein-protein interaction databases

BioGridi36445. 74 interactions.
DIPiDIP-2026N.
IntActiP32793. 86 interactions.
MINTiMINT-374921.
STRINGi4932.YHR016C.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi413 – 4186
Beta strandi435 – 4406
Beta strandi447 – 4537
Beta strandi456 – 4616
Helixi462 – 4643
Beta strandi465 – 4673

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2A08X-ray1.54A/B413-468[»]
ProteinModelPortaliP32793.
SMRiP32793. Positions 413-468.

Miscellaneous databases

EvolutionaryTraceiP32793.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini409 – 46860SH3
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi222 – 30786Asp-rich
Add
BLAST

Sequence similaritiesi

Belongs to the SH3YL1 family.
Contains 1 SH3 domain.

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiCOG2930.
GeneTreeiENSGT00510000048137.
HOGENOMiHOG000215564.
OMAiERKEANS.
OrthoDBiEOG7X9GH6.

Family and domain databases

InterProiIPR001452. SH3_domain.
IPR013315. Spectrin_alpha_SH3.
IPR007461. Ysc84_actin-binding.
[Graphical view]
PfamiPF04366. DUF500. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
PR01887. SPECTRNALPHA.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32793-1 [UniParc]FASTAAdd to Basket

« Hide

MGINNPIPRS LKSETKKAAK VLRSFVKPNQ VFGADQVIPP YVLKRAKGLA    50
IITVLKAGFL FSGRAGSGVI VARLKDGTWS APSAIAMAGA GAGGMVGVEL 100
TDFVFILNSE EAVRSFSEFG TITLGGNVSV SAGPLGRSAE AAASASTGGV 150
SAVFAYSKSK GLFAGVSVEG SAILERREAN RKFYGDNCTS KMILSGRVKV 200
PPAADPLLRI LESRAFNFTR HDHDDNASGD DFYDDGQYSD NTSHYYDDIP 250
DSFDSTDESS TRPNTRSSRR RGMSLGSRSR YDDDYDDDGY GRGRGYGDFD 300
SEDEDYDYGR SPNRNSSRNR GPQIDRGTKP RANTRWEDDL YDRDTEYSRP 350
NHSGRDYDNT RGNRRGYGRE RGYSLGHGPT HPSNMSNVDD LSHKMSKTGL 400
GNESTATNSA TPTAVALYNF AGEQPGDLAF KKGDVITILK KSDSQNDWWT 450
GRTNGKEGIF PANYVRVS 468
Length:468
Mass (Da):50,902
Last modified:April 18, 2006 - v2
Checksum:iDE42E8ECF5B83C92
GO

Sequence cautioni

The sequence AAA56990.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U10400 Genomic DNA. Translation: AAB68945.1.
L06795 Genomic DNA. Translation: AAA56990.1. Sequence problems.
BK006934 Genomic DNA. Translation: DAA06705.1.
PIRiS46791.
RefSeqiNP_011880.1. NM_001179146.1.

Genome annotation databases

EnsemblFungiiYHR016C; YHR016C; YHR016C.
GeneIDi856409.
KEGGisce:YHR016C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U10400 Genomic DNA. Translation: AAB68945.1 .
L06795 Genomic DNA. Translation: AAA56990.1 . Sequence problems.
BK006934 Genomic DNA. Translation: DAA06705.1 .
PIRi S46791.
RefSeqi NP_011880.1. NM_001179146.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2A08 X-ray 1.54 A/B 413-468 [» ]
ProteinModelPortali P32793.
SMRi P32793. Positions 413-468.
ModBasei Search...

Protein-protein interaction databases

BioGridi 36445. 74 interactions.
DIPi DIP-2026N.
IntActi P32793. 86 interactions.
MINTi MINT-374921.
STRINGi 4932.YHR016C.

Proteomic databases

MaxQBi P32793.
PaxDbi P32793.
PeptideAtlasi P32793.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YHR016C ; YHR016C ; YHR016C .
GeneIDi 856409.
KEGGi sce:YHR016C.

Organism-specific databases

CYGDi YHR016c.
SGDi S000001058. YSC84.

Phylogenomic databases

eggNOGi COG2930.
GeneTreei ENSGT00510000048137.
HOGENOMi HOG000215564.
OMAi ERKEANS.
OrthoDBi EOG7X9GH6.

Enzyme and pathway databases

BioCyci YEAST:G3O-31078-MONOMER.

Miscellaneous databases

EvolutionaryTracei P32793.
NextBioi 981956.

Gene expression databases

Genevestigatori P32793.

Family and domain databases

InterProi IPR001452. SH3_domain.
IPR013315. Spectrin_alpha_SH3.
IPR007461. Ysc84_actin-binding.
[Graphical view ]
Pfami PF04366. DUF500. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view ]
PRINTSi PR00452. SH3DOMAIN.
PR01887. SPECTRNALPHA.
SMARTi SM00326. SH3. 1 hit.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 1 hit.
PROSITEi PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of two divergently transcribed genes centromere-proximal to the ARG4 locus on chromosome VIII of Saccharomyces cerevisiae."
    Rocco V., Daly M.J., Matre V., Lichten M., Nicolas A.
    Yeast 9:1111-1120(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The Saccharomyces cerevisiae homologue of human Wiskott-Aldrich syndrome protein Las17p interacts with the Arp2/3 complex."
    Madania A., Dumoulin P., Grava S., Kitamoto H., Scharer-Brodbeck C., Soulard A., Moreau V., Winsor B.
    Mol. Biol. Cell 10:3521-3538(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LAS17.
  5. "Novel proteins linking the actin cytoskeleton to the endocytic machinery in Saccharomyces cerevisiae."
    Dewar H., Warren D.T., Gardiner F.C., Gourlay C.G., Satish N., Richardson M.R., Andrews P.D., Ayscough K.R.
    Mol. Biol. Cell 13:3646-3661(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SLA1, SUBCELLULAR LOCATION.
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-301, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-301 AND SER-311, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-301 AND SER-311, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiYSC84_YEAST
AccessioniPrimary (citable) accession number: P32793
Secondary accession number(s): D3DKW1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: April 18, 2006
Last modified: June 11, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3

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