Protein YSC84 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P32793 (YSC84_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 117. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein YSC84

Alternative name(s):
LAS seventeen-binding protein 4
Short name=LAS17-binding protein 4

Gene names

Name:	YSC84
Synonyms:	LSB4
Ordered Locus Names:	YHR016C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for the organization of the actin cytoskeleton, fluid phase endocytosis and vesicle trafficking, together with LSB5. Ref.5
Subunit structure	Interacts with LAS17 and SLA1. Ref.4 Ref.5
Subcellular location	Cytoplasm › cytoskeleton › actin patch. Note: Cortical actin patches. Ref.5
Induction	Induced during sporulation. Ref.1
Sequence similarities	Belongs to the SH3YL1 family. Contains 1 SH3 domain.
Sequence caution	The sequence AAA56990.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Domain	SH3 domain
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin cortical patch localization Inferred from mutant phenotype PubMed 19158382. Source: SGD actin filament bundle assembly Inferred from direct assay PubMed 19158382. Source: SGD endocytosis Inferred from genetic interaction Ref.5. Source: SGD
Cellular_component	actin cortical patch Inferred from direct assay Ref.5. Source: SGD
Molecular_function	actin filament binding Inferred from direct assay PubMed 19158382. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
ABP1	P15891	2	EBI-24460,EBI-2036
ACF2	Q12168	4	EBI-24460,EBI-32973
ACF4	P47129	3	EBI-24460,EBI-25556
AIM3	P38266	3	EBI-24460,EBI-21584
APP1	P53933	4	EBI-24460,EBI-28798
BSP1	Q06604	3	EBI-24460,EBI-37047
CUE5	Q08412	3	EBI-24460,EBI-37580
ERT1	P38140	2	EBI-24460,EBI-21048
GTS1	P40956	2	EBI-24460,EBI-7968
GYL1	Q04322	3	EBI-24460,EBI-27427
LAS17	Q12446	12	EBI-24460,EBI-10022
SLA1	P32790	6	EBI-24460,EBI-17313

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 468

468

Protein YSC84

PRO_0000202887

Regions

Domain

409 – 468

SH3

Compositional bias

222 – 307

Asp-rich

Amino acid modifications

Modified residue

301

Phosphoserine Ref.6 Ref.7 Ref.8 Ref.9

Modified residue

311

Phosphoserine Ref.9

Modified residue

374

Phosphoserine Ref.8 Ref.9

Secondary structure

468

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P32793 [UniParc].

Last modified April 18, 2006. Version 2.
Checksum: DE42E8ECF5B83C92
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FASTA

468

50,902

        10         20         30         40         50         60 
MGINNPIPRS LKSETKKAAK VLRSFVKPNQ VFGADQVIPP YVLKRAKGLA IITVLKAGFL 

        70         80         90        100        110        120 
FSGRAGSGVI VARLKDGTWS APSAIAMAGA GAGGMVGVEL TDFVFILNSE EAVRSFSEFG 

       130        140        150        160        170        180 
TITLGGNVSV SAGPLGRSAE AAASASTGGV SAVFAYSKSK GLFAGVSVEG SAILERREAN 

       190        200        210        220        230        240 
RKFYGDNCTS KMILSGRVKV PPAADPLLRI LESRAFNFTR HDHDDNASGD DFYDDGQYSD 

       250        260        270        280        290        300 
NTSHYYDDIP DSFDSTDESS TRPNTRSSRR RGMSLGSRSR YDDDYDDDGY GRGRGYGDFD 

       310        320        330        340        350        360 
SEDEDYDYGR SPNRNSSRNR GPQIDRGTKP RANTRWEDDL YDRDTEYSRP NHSGRDYDNT 

       370        380        390        400        410        420 
RGNRRGYGRE RGYSLGHGPT HPSNMSNVDD LSHKMSKTGL GNESTATNSA TPTAVALYNF 

       430        440        450        460 
AGEQPGDLAF KKGDVITILK KSDSQNDWWT GRTNGKEGIF PANYVRVS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of two divergently transcribed genes centromere-proximal to the ARG4 locus on chromosome VIII of Saccharomyces cerevisiae." Rocco V., Daly M.J., Matre V., Lichten M., Nicolas A. Yeast 9:1111-1120(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The Saccharomyces cerevisiae homologue of human Wiskott-Aldrich syndrome protein Las17p interacts with the Arp2/3 complex." Madania A., Dumoulin P., Grava S., Kitamoto H., Scharer-Brodbeck C., Soulard A., Moreau V., Winsor B. Mol. Biol. Cell 10:3521-3538(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH LAS17.
[5]	"Novel proteins linking the actin cytoskeleton to the endocytic machinery in Saccharomyces cerevisiae." Dewar H., Warren D.T., Gardiner F.C., Gourlay C.G., Satish N., Richardson M.R., Andrews P.D., Ayscough K.R. Mol. Biol. Cell 13:3646-3661(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SLA1, SUBCELLULAR LOCATION.
[6]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-301, MASS SPECTROMETRY. Strain: 2124.
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-301, MASS SPECTROMETRY. Strain: ADR376.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-301 AND SER-374, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-301; SER-311 AND SER-374, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U10400 Genomic DNA. Translation: AAB68945.1.
L06795 Genomic DNA. Translation: AAA56990.1. Sequence problems.
BK006934 Genomic DNA. Translation: DAA06705.1.

PIR

S46791.

RefSeq

NP_011880.1. NM_001179146.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2A08	X-ray	1.54	A/B	413-468	[»]

ProteinModelPortal

P32793.

SMR

P32793. Positions 413-468.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-2026N.

IntAct

P32793. 51 interactions.

MINT

MINT-374921.

STRING

4932.YHR016C.

Proteomic databases

PaxDb

P32793.

PeptideAtlas

P32793.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YHR016C; YHR016C; YHR016C.

GeneID

856409.

KEGG

sce:YHR016C.

Organism-specific databases

CYGD

YHR016c.

SGD

S000001058. YSC84.

Phylogenomic databases

eggNOG

COG2930.

GeneTree

ENSGT00510000048137.

HOGENOM

HOG000215564.

OMA

MERRGAN.

OrthoDB

EOG4643NB.

Gene expression databases

Genevestigator

P32793.

GermOnline

YHR016C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR001452. SH3_domain.
IPR013315. Spectrin_alpha_SH3.
IPR007461. Ysc84_actin-binding.
[Graphical view]

Pfam

PF04366. DUF500. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]

PRINTS

PR00452. SH3DOMAIN.
PR01887. SPECTRNALPHA.

SMART

SM00326. SH3. 1 hit.
[Graphical view]

SUPFAM

SSF50044. SH3. 1 hit.

PROSITE

PS50002. SH3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P32793.

NextBio

981956.

Entry information

Entry name

YSC84_YEAST

Accession

Primary (citable) accession number: P32793
Secondary accession number(s): D3DKW1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	April 18, 2006
Last modified:	April 3, 2013
This is version 117 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents