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Protein

Protein YSC84

Gene

YSC84

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential for the organization of the actin cytoskeleton, fluid phase endocytosis and vesicle trafficking, together with LSB5.1 Publication

GO - Molecular functioni

  1. actin filament binding Source: SGD

GO - Biological processi

  1. actin cortical patch localization Source: SGD
  2. actin cytoskeleton organization Source: SGD
  3. actin filament bundle assembly Source: SGD
  4. actin filament organization Source: SGD
  5. endocytosis Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-31078-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein YSC84
Alternative name(s):
LAS seventeen-binding protein 4
Short name:
LAS17-binding protein 4
Gene namesi
Name:YSC84
Synonyms:LSB4
Ordered Locus Names:YHR016C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHR016c.
SGDiS000001058. YSC84.

Subcellular locationi

Cytoplasmcytoskeletonactin patch 1 Publication
Note: Cortical actin patches.

GO - Cellular componenti

  1. actin cortical patch Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 468468Protein YSC84PRO_0000202887Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei301 – 3011Phosphoserine3 Publications
Modified residuei311 – 3111Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32793.
PaxDbiP32793.
PeptideAtlasiP32793.

Expressioni

Inductioni

Induced during sporulation.1 Publication

Gene expression databases

GenevestigatoriP32793.

Interactioni

Subunit structurei

Interacts with LAS17 and SLA1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ABP1P158913EBI-24460,EBI-2036
ACF2Q121687EBI-24460,EBI-32973
ACF4P471294EBI-24460,EBI-25556
AGP2P380902EBI-24460,EBI-2362
AIM3P382665EBI-24460,EBI-21584
APP1P539336EBI-24460,EBI-28798
BSP1Q066046EBI-24460,EBI-37047
CMR1Q125102EBI-24460,EBI-35343
CUE5Q084127EBI-24460,EBI-37580
ERT1P381404EBI-24460,EBI-21048
GTS1P409564EBI-24460,EBI-7968
GYL1Q043226EBI-24460,EBI-27427
GYP5Q123442EBI-24460,EBI-38508
HUA1P403253EBI-24460,EBI-23614
LAS17Q124467EBI-24460,EBI-10022
NBA1Q082292EBI-24460,EBI-36841
POM33Q121642EBI-24460,EBI-30000
RIM101P334002EBI-24460,EBI-14422
SEC8P328554EBI-24460,EBI-16896
SLA1P327908EBI-24460,EBI-17313
STP22P256042EBI-24460,EBI-411625

Protein-protein interaction databases

BioGridi36445. 75 interactions.
DIPiDIP-2026N.
IntActiP32793. 86 interactions.
MINTiMINT-374921.
STRINGi4932.YHR016C.

Structurei

Secondary structure

1
468
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi413 – 4186Combined sources
Beta strandi435 – 4406Combined sources
Beta strandi447 – 4537Combined sources
Beta strandi456 – 4616Combined sources
Helixi462 – 4643Combined sources
Beta strandi465 – 4673Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2A08X-ray1.54A/B413-468[»]
ProteinModelPortaliP32793.
SMRiP32793. Positions 413-468.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32793.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini409 – 46860SH3PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi222 – 30786Asp-richAdd
BLAST

Sequence similaritiesi

Belongs to the SH3YL1 family.Curated
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiCOG2930.
GeneTreeiENSGT00510000048137.
HOGENOMiHOG000215564.
InParanoidiP32793.
OMAiERKEANS.
OrthoDBiEOG7X9GH6.

Family and domain databases

InterProiIPR001452. SH3_domain.
IPR013315. Spectrin_alpha_SH3.
IPR007461. Ysc84_actin-binding.
[Graphical view]
PfamiPF04366. DUF500. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
PR01887. SPECTRNALPHA.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32793-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGINNPIPRS LKSETKKAAK VLRSFVKPNQ VFGADQVIPP YVLKRAKGLA
60 70 80 90 100
IITVLKAGFL FSGRAGSGVI VARLKDGTWS APSAIAMAGA GAGGMVGVEL
110 120 130 140 150
TDFVFILNSE EAVRSFSEFG TITLGGNVSV SAGPLGRSAE AAASASTGGV
160 170 180 190 200
SAVFAYSKSK GLFAGVSVEG SAILERREAN RKFYGDNCTS KMILSGRVKV
210 220 230 240 250
PPAADPLLRI LESRAFNFTR HDHDDNASGD DFYDDGQYSD NTSHYYDDIP
260 270 280 290 300
DSFDSTDESS TRPNTRSSRR RGMSLGSRSR YDDDYDDDGY GRGRGYGDFD
310 320 330 340 350
SEDEDYDYGR SPNRNSSRNR GPQIDRGTKP RANTRWEDDL YDRDTEYSRP
360 370 380 390 400
NHSGRDYDNT RGNRRGYGRE RGYSLGHGPT HPSNMSNVDD LSHKMSKTGL
410 420 430 440 450
GNESTATNSA TPTAVALYNF AGEQPGDLAF KKGDVITILK KSDSQNDWWT
460
GRTNGKEGIF PANYVRVS
Length:468
Mass (Da):50,902
Last modified:April 18, 2006 - v2
Checksum:iDE42E8ECF5B83C92
GO

Sequence cautioni

The sequence AAA56990.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10400 Genomic DNA. Translation: AAB68945.1.
L06795 Genomic DNA. Translation: AAA56990.1. Sequence problems.
BK006934 Genomic DNA. Translation: DAA06705.1.
PIRiS46791.
RefSeqiNP_011880.1. NM_001179146.1.

Genome annotation databases

EnsemblFungiiYHR016C; YHR016C; YHR016C.
GeneIDi856409.
KEGGisce:YHR016C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10400 Genomic DNA. Translation: AAB68945.1.
L06795 Genomic DNA. Translation: AAA56990.1. Sequence problems.
BK006934 Genomic DNA. Translation: DAA06705.1.
PIRiS46791.
RefSeqiNP_011880.1. NM_001179146.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2A08X-ray1.54A/B413-468[»]
ProteinModelPortaliP32793.
SMRiP32793. Positions 413-468.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36445. 75 interactions.
DIPiDIP-2026N.
IntActiP32793. 86 interactions.
MINTiMINT-374921.
STRINGi4932.YHR016C.

Proteomic databases

MaxQBiP32793.
PaxDbiP32793.
PeptideAtlasiP32793.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR016C; YHR016C; YHR016C.
GeneIDi856409.
KEGGisce:YHR016C.

Organism-specific databases

CYGDiYHR016c.
SGDiS000001058. YSC84.

Phylogenomic databases

eggNOGiCOG2930.
GeneTreeiENSGT00510000048137.
HOGENOMiHOG000215564.
InParanoidiP32793.
OMAiERKEANS.
OrthoDBiEOG7X9GH6.

Enzyme and pathway databases

BioCyciYEAST:G3O-31078-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP32793.
NextBioi981956.

Gene expression databases

GenevestigatoriP32793.

Family and domain databases

InterProiIPR001452. SH3_domain.
IPR013315. Spectrin_alpha_SH3.
IPR007461. Ysc84_actin-binding.
[Graphical view]
PfamiPF04366. DUF500. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
PR01887. SPECTRNALPHA.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of two divergently transcribed genes centromere-proximal to the ARG4 locus on chromosome VIII of Saccharomyces cerevisiae."
    Rocco V., Daly M.J., Matre V., Lichten M., Nicolas A.
    Yeast 9:1111-1120(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The Saccharomyces cerevisiae homologue of human Wiskott-Aldrich syndrome protein Las17p interacts with the Arp2/3 complex."
    Madania A., Dumoulin P., Grava S., Kitamoto H., Scharer-Brodbeck C., Soulard A., Moreau V., Winsor B.
    Mol. Biol. Cell 10:3521-3538(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LAS17.
  5. "Novel proteins linking the actin cytoskeleton to the endocytic machinery in Saccharomyces cerevisiae."
    Dewar H., Warren D.T., Gardiner F.C., Gourlay C.G., Satish N., Richardson M.R., Andrews P.D., Ayscough K.R.
    Mol. Biol. Cell 13:3646-3661(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SLA1, SUBCELLULAR LOCATION.
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-301, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-301 AND SER-311, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-301 AND SER-311, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiYSC84_YEAST
AccessioniPrimary (citable) accession number: P32793
Secondary accession number(s): D3DKW1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: April 18, 2006
Last modified: March 4, 2015
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.