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P32793 (YSC84_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein YSC84
Alternative name(s):
LAS seventeen-binding protein 4
Short name=LAS17-binding protein 4
Gene names
Name:YSC84
Synonyms:LSB4
Ordered Locus Names:YHR016C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length468 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential for the organization of the actin cytoskeleton, fluid phase endocytosis and vesicle trafficking, together with LSB5. Ref.5

Subunit structure

Interacts with LAS17 and SLA1. Ref.4 Ref.5

Subcellular location

Cytoplasmcytoskeletonactin patch. Note: Cortical actin patches. Ref.5

Induction

Induced during sporulation. Ref.1

Sequence similarities

Belongs to the SH3YL1 family.

Contains 1 SH3 domain.

Sequence caution

The sequence AAA56990.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 468468Protein YSC84
PRO_0000202887

Regions

Domain409 – 46860SH3
Compositional bias222 – 30786Asp-rich

Amino acid modifications

Modified residue3011Phosphoserine Ref.6 Ref.7 Ref.8
Modified residue3111Phosphoserine Ref.7 Ref.8

Secondary structure

........... 468
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P32793 [UniParc].

Last modified April 18, 2006. Version 2.
Checksum: DE42E8ECF5B83C92

FASTA46850,902
        10         20         30         40         50         60 
MGINNPIPRS LKSETKKAAK VLRSFVKPNQ VFGADQVIPP YVLKRAKGLA IITVLKAGFL 

        70         80         90        100        110        120 
FSGRAGSGVI VARLKDGTWS APSAIAMAGA GAGGMVGVEL TDFVFILNSE EAVRSFSEFG 

       130        140        150        160        170        180 
TITLGGNVSV SAGPLGRSAE AAASASTGGV SAVFAYSKSK GLFAGVSVEG SAILERREAN 

       190        200        210        220        230        240 
RKFYGDNCTS KMILSGRVKV PPAADPLLRI LESRAFNFTR HDHDDNASGD DFYDDGQYSD 

       250        260        270        280        290        300 
NTSHYYDDIP DSFDSTDESS TRPNTRSSRR RGMSLGSRSR YDDDYDDDGY GRGRGYGDFD 

       310        320        330        340        350        360 
SEDEDYDYGR SPNRNSSRNR GPQIDRGTKP RANTRWEDDL YDRDTEYSRP NHSGRDYDNT 

       370        380        390        400        410        420 
RGNRRGYGRE RGYSLGHGPT HPSNMSNVDD LSHKMSKTGL GNESTATNSA TPTAVALYNF 

       430        440        450        460 
AGEQPGDLAF KKGDVITILK KSDSQNDWWT GRTNGKEGIF PANYVRVS 

« Hide

References

« Hide 'large scale' references
[1]"Identification of two divergently transcribed genes centromere-proximal to the ARG4 locus on chromosome VIII of Saccharomyces cerevisiae."
Rocco V., Daly M.J., Matre V., Lichten M., Nicolas A.
Yeast 9:1111-1120(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"The Saccharomyces cerevisiae homologue of human Wiskott-Aldrich syndrome protein Las17p interacts with the Arp2/3 complex."
Madania A., Dumoulin P., Grava S., Kitamoto H., Scharer-Brodbeck C., Soulard A., Moreau V., Winsor B.
Mol. Biol. Cell 10:3521-3538(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH LAS17.
[5]"Novel proteins linking the actin cytoskeleton to the endocytic machinery in Saccharomyces cerevisiae."
Dewar H., Warren D.T., Gardiner F.C., Gourlay C.G., Satish N., Richardson M.R., Andrews P.D., Ayscough K.R.
Mol. Biol. Cell 13:3646-3661(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SLA1, SUBCELLULAR LOCATION.
[6]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-301, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-301 AND SER-311, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-301 AND SER-311, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U10400 Genomic DNA. Translation: AAB68945.1.
L06795 Genomic DNA. Translation: AAA56990.1. Sequence problems.
BK006934 Genomic DNA. Translation: DAA06705.1.
PIRS46791.
RefSeqNP_011880.1. NM_001179146.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2A08X-ray1.54A/B413-468[»]
ProteinModelPortalP32793.
SMRP32793. Positions 413-468.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36445. 74 interactions.
DIPDIP-2026N.
IntActP32793. 86 interactions.
MINTMINT-374921.
STRING4932.YHR016C.

Proteomic databases

PaxDbP32793.
PeptideAtlasP32793.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHR016C; YHR016C; YHR016C.
GeneID856409.
KEGGsce:YHR016C.

Organism-specific databases

CYGDYHR016c.
SGDS000001058. YSC84.

Phylogenomic databases

eggNOGCOG2930.
GeneTreeENSGT00510000048137.
HOGENOMHOG000215564.
OMAERKEANS.
OrthoDBEOG7X9GH6.

Enzyme and pathway databases

BioCycYEAST:G3O-31078-MONOMER.

Gene expression databases

GenevestigatorP32793.

Family and domain databases

InterProIPR001452. SH3_domain.
IPR013315. Spectrin_alpha_SH3.
IPR007461. Ysc84_actin-binding.
[Graphical view]
PfamPF04366. DUF500. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSPR00452. SH3DOMAIN.
PR01887. SPECTRNALPHA.
SMARTSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF50044. SSF50044. 1 hit.
PROSITEPS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP32793.
NextBio981956.

Entry information

Entry nameYSC84_YEAST
AccessionPrimary (citable) accession number: P32793
Secondary accession number(s): D3DKW1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: April 18, 2006
Last modified: April 16, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references