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Protein

Actin cytoskeleton-regulatory complex protein SLA1

Gene

SLA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the PAN1 actin cytoskeleton-regulatory complex required for the internalization of endosomes during actin-coupled endocytosis. The complex links the site of endocytosis to the cell membrane-associated actin cytoskeleton. Mediates uptake of external molecules and vacuolar degradation of plasma membrane proteins. Plays a role in the proper organization of the cell membrane-associated actin cytoskeleton and promotes its destabilization.15 Publications

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • protein binding, bridging Source: SGD
  • ubiquitin binding Source: SGD

GO - Biological processi

  • actin cortical patch assembly Source: SGD
  • actin filament polymerization Source: SGD
  • endocytosis Source: SGD
Complete GO annotation...

Keywords - Biological processi

Endocytosis

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-28913-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Actin cytoskeleton-regulatory complex protein SLA1
Gene namesi
Name:SLA1
Ordered Locus Names:YBL007C
ORF Names:YBL0321
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBL007C.
SGDiS000000103. SLA1.

Subcellular locationi

GO - Cellular componenti

  • actin cortical patch Source: SGD
  • cell cortex Source: SGD
  • endosome membrane Source: UniProtKB-SubCell
  • nucleus Source: SGD
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Endosome, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12441244Actin cytoskeleton-regulatory complex protein SLA1PRO_0000071943Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei447 – 4471PhosphoserineCombined sources
Modified residuei449 – 4491PhosphoserineCombined sources
Modified residuei454 – 4541PhosphoserineCombined sources
Cross-linki471 – 471Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki548 – 548Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei799 – 7991PhosphoserineCombined sources
Modified residuei831 – 8311PhosphothreonineCombined sources
Modified residuei858 – 8581PhosphothreonineCombined sources
Modified residuei887 – 8871PhosphothreonineCombined sources
Modified residuei904 – 9041PhosphothreonineCombined sources
Modified residuei984 – 9841PhosphothreonineCombined sources
Modified residuei993 – 9931PhosphothreonineCombined sources
Modified residuei996 – 9961PhosphoserineCombined sources
Modified residuei1075 – 10751PhosphothreonineCombined sources

Post-translational modificationi

Phosphorylated by PRK1.2 Publications

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP32790.

PTM databases

iPTMnetiP32790.

Interactioni

Subunit structurei

Component of the PAN1 actin cytoskeleton-regulatory complex. Interacts with ABP1, KRE6, LAS17, LSB5, RSP5, RVS167, VPS1 and YSC84.10 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself11EBI-17313,EBI-17313
ABP1P158914EBI-17313,EBI-2036
AIM21P405634EBI-17313,EBI-25376
APP1P539334EBI-17313,EBI-28798
BSP1Q066046EBI-17313,EBI-37047
CHC1P221374EBI-17313,EBI-4766
ECM25P325255EBI-17313,EBI-26215
END3P390134EBI-17313,EBI-6460
INP52P509423EBI-17313,EBI-28834
KEX2P1313416EBI-17313,EBI-9658
LAS17Q124466EBI-17313,EBI-10022
LSB3P436038EBI-17313,EBI-22980
LSB5P253695EBI-17313,EBI-10218
MPS1P541992EBI-17313,EBI-11224
PAN1P325215EBI-17313,EBI-12875
RRP7P253683EBI-17313,EBI-16019
STD1Q027943EBI-17313,EBI-18344
SYP1P256233EBI-17313,EBI-21900
VRP1P373703EBI-17313,EBI-20502
YSC84P327938EBI-17313,EBI-24460
ZDS2P547863EBI-17313,EBI-29637

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • protein binding, bridging Source: SGD
  • ubiquitin binding Source: SGD

Protein-protein interaction databases

BioGridi32692. 423 interactions.
DIPiDIP-695N.
IntActiP32790. 157 interactions.
MINTiMINT-410065.

Structurei

Secondary structure

1
1244
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi357 – 3604Combined sources
Beta strandi379 – 3879Combined sources
Beta strandi389 – 3968Combined sources
Turni397 – 3993Combined sources
Beta strandi402 – 4065Combined sources
Helixi407 – 4093Combined sources
Beta strandi410 – 4123Combined sources
Turni413 – 4153Combined sources
Beta strandi497 – 5059Combined sources
Beta strandi508 – 51710Combined sources
Beta strandi520 – 5245Combined sources
Beta strandi530 – 5345Combined sources
Helixi540 – 55011Combined sources
Helixi555 – 5573Combined sources
Helixi657 – 6637Combined sources
Helixi668 – 68013Combined sources
Helixi685 – 6906Combined sources
Helixi693 – 6986Combined sources
Helixi703 – 71614Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SSHX-ray1.40B191-202[»]
1Z9ZX-ray1.95A/B357-413[»]
2HBPNMR-A495-560[»]
2JT4NMR-A350-420[»]
2V1QX-ray1.20A/B357-413[»]
3IDWX-ray1.85A653-724[»]
ProteinModelPortaliP32790.
SMRiP32790. Positions 7-67, 76-129, 350-420, 495-560, 654-719.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32790.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 6962SH3 1PROSITE-ProRule annotationAdd
BLAST
Domaini70 – 13263SH3 2PROSITE-ProRule annotationAdd
BLAST
Domaini353 – 41563SH3 3PROSITE-ProRule annotationAdd
BLAST
Repeati868 – 87471
Repeati877 – 88372
Repeati887 – 89373
Repeati923 – 92974
Repeati945 – 95175
Repeati1003 – 100976
Repeati1020 – 102677
Repeati1031 – 103778
Repeati1048 – 105479
Repeati1065 – 1071710
Repeati1084 – 1090711
Repeati1129 – 1135712
Repeati1155 – 1161713
Repeati1170 – 1176714
Repeati1185 – 1191715
Repeati1200 – 1206716

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni868 – 120533816 X 7 AA approximate repeats of T-G-G-A-M-M-PAdd
BLAST

Sequence similaritiesi

Belongs to the SLA1 family.Curated
Contains 3 SH3 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

GeneTreeiENSGT00730000114400.
HOGENOMiHOG000157527.
InParanoidiP32790.
KOiK20046.
OMAiSFGAQMT.
OrthoDBiEOG092C14N7.

Family and domain databases

InterProiIPR001452. SH3_domain.
IPR007131. SHD1.
[Graphical view]
PfamiPF00018. SH3_1. 2 hits.
PF14604. SH3_9. 1 hit.
PF03983. SHD1. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 3 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 3 hits.
PROSITEiPS50002. SH3. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32790-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVFLGIYRA VYAYEPQTPE ELAIQEDDLL YLLQKSDIDD WWTVKKRVIG
60 70 80 90 100
SDSEEPVGLV PSTYIEEAPV LKKVRAIYDY EQVQNADEEL TFHENDVFDV
110 120 130 140 150
FDDKDADWLL VKSTVSNEFG FIPGNYVEPE NGSTSKQEQA PAAAEAPAAT
160 170 180 190 200
PAAAPASAAV LPTNFLPPPQ HNDRARMMQS KEDQAPDEDE EGPPPAMPAR
210 220 230 240 250
PTATTETTDA TAAAVRSRTR LSYSDNDNDD EEDDYYYNSN SNNVGNHEYN
260 270 280 290 300
TEYHSWNVTE IEGRKKKKAK LSIGNNKINF IPQKGTPHEW SIDKLVSYDN
310 320 330 340 350
EKKHMFLEFV DPYRSLELHT GNTTTCEEIM NIIGEYKGAS RDPGLREVEM
360 370 380 390 400
ASKSKKRGIV QYDFMAESQD ELTIKSGDKV YILDDKKSKD WWMCQLVDSG
410 420 430 440 450
KSGLVPAQFI EPVRDKKHTE STASGIIKSI KKNFTKSPSR SRSRSRSKSN
460 470 480 490 500
ANASWKDDEL QNDVVGSAAG KRSRKSSLSS HKKNSSATKD FPNPKKSRLW
510 520 530 540 550
VDRSGTFKVD AEFIGCAKGK IHLHKANGVK IAVAADKLSN EDLAYVEKIT
560 570 580 590 600
GFSLEKFKAN DGSSSRGTDS RDSERERRRR LKEQEEKERD RRLKERELYE
610 620 630 640 650
LKKARELLDE ERSRLQEKEL PPIKPPRPTS TTSVPNTTSV PPAESSNNNN
660 670 680 690 700
SSNKYDWFEF FLNCGVDVSN CQRYTINFDR EQLTEDMMPD INNSMLRTLG
710 720 730 740 750
LREGDIVRVM KHLDKKFGRE NIASIPTNAT GNMFSQPDGS LNVATSPETS
760 770 780 790 800
LPQQLLPQTT SPAQTAPSTS AETDDAWTVK PASKSESNLL SKKSEFTGSM
810 820 830 840 850
QDLLDLQPLE PKKAAASTPE PNLKDLEPVK TGGTTVPAAP VSSAPVSSAP
860 870 880 890 900
APLDPFKTGG NNILPLSTGF VMMPMITGGD MLPMQRTGGF VVPQTTFGMQ
910 920 930 940 950
SQVTGGILPV QKTGNGLIPI SNTGGAMMPQ TTFGAAATVL PLQKTGGGLI
960 970 980 990 1000
PIATTGGAQF PQTSFNVQGQ QQLPTGSILP VQKTANGLIS ANTGVSMPTV
1010 1020 1030 1040 1050
QRTGGTMIPQ TSFGVSQQLT GGAMMTQPQN TGSAMMPQTS FNAVPQITGG
1060 1070 1080 1090 1100
AMMPQTSFNA LPQVTGGAMM PLQRTGGALN TFNTGGAMIP QTSFSSQAQN
1110 1120 1130 1140 1150
TGGFRPQSQF GLTLQKTGGI APLNQNQFTG GAMNTLSTGG VLQQQQPQTM
1160 1170 1180 1190 1200
NTFNTGGVMQ ELQMMTTFNT GGAMQQPQMM NTFNTDGIMQ QPQMMNTFNT
1210 1220 1230 1240
GGAMQQPQQQ ALQNQPTGFG FGNGPQQSRQ ANIFNATASN PFGF
Length:1,244
Mass (Da):135,848
Last modified:October 1, 1993 - v1
Checksum:i7FD85AA776407624
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22810 Genomic DNA. Translation: CAA80463.1.
Z35768 Genomic DNA. Translation: CAA84826.1.
S47695 Genomic DNA. Translation: AAB23985.1.
BK006936 Genomic DNA. Translation: DAA07113.1.
PIRiS25327.
RefSeqiNP_009546.1. NM_001178247.1.

Genome annotation databases

EnsemblFungiiYBL007C; YBL007C; YBL007C.
GeneIDi852276.
KEGGisce:YBL007C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22810 Genomic DNA. Translation: CAA80463.1.
Z35768 Genomic DNA. Translation: CAA84826.1.
S47695 Genomic DNA. Translation: AAB23985.1.
BK006936 Genomic DNA. Translation: DAA07113.1.
PIRiS25327.
RefSeqiNP_009546.1. NM_001178247.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SSHX-ray1.40B191-202[»]
1Z9ZX-ray1.95A/B357-413[»]
2HBPNMR-A495-560[»]
2JT4NMR-A350-420[»]
2V1QX-ray1.20A/B357-413[»]
3IDWX-ray1.85A653-724[»]
ProteinModelPortaliP32790.
SMRiP32790. Positions 7-67, 76-129, 350-420, 495-560, 654-719.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32692. 423 interactions.
DIPiDIP-695N.
IntActiP32790. 157 interactions.
MINTiMINT-410065.

PTM databases

iPTMnetiP32790.

Proteomic databases

MaxQBiP32790.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBL007C; YBL007C; YBL007C.
GeneIDi852276.
KEGGisce:YBL007C.

Organism-specific databases

EuPathDBiFungiDB:YBL007C.
SGDiS000000103. SLA1.

Phylogenomic databases

GeneTreeiENSGT00730000114400.
HOGENOMiHOG000157527.
InParanoidiP32790.
KOiK20046.
OMAiSFGAQMT.
OrthoDBiEOG092C14N7.

Enzyme and pathway databases

BioCyciYEAST:G3O-28913-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP32790.
PROiP32790.

Family and domain databases

InterProiIPR001452. SH3_domain.
IPR007131. SHD1.
[Graphical view]
PfamiPF00018. SH3_1. 2 hits.
PF14604. SH3_9. 1 hit.
PF03983. SHD1. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 3 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 3 hits.
PROSITEiPS50002. SH3. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSLA1_YEAST
AccessioniPrimary (citable) accession number: P32790
Secondary accession number(s): D6VPZ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: September 7, 2016
This is version 169 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 952 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.