Reviewed, UniProtKB/Swiss-Prot P32789 (ALK2_YEAST)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase Haspin homolog ALK2
    EC=2.7.11.1

Gene names

Name:	ALK2
Ordered Locus Names:	YBL009W
ORF Names:	YBL0317

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	676 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Serine/threonine haspin-like protein kinase involved in cell cycle regulation. Ref.3
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Induction	Absent in G1-arrested cells and accumulates in G2-M. Ref.3
Domain	The KEN and D (destructive) boxes are required for the cell cycle-controled ALK2 degradation by the anaphase promoting complex (APC) pathway. Ref.3 The protein kinase domain is predicted to be catalytically inactive. However, weak kinase activity was experimentally demonstrated. Ref.3
Post-translational modification	Periodically phosphorylated during the cell cycle with a phosphorylation peak during mitosis and hyperphosphorylated after DNA damage. Ref.3
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Haspin subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle DNA damage
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	meiosis Inferred from expression pattern. Source: SGD mitosis Ref.3 Inferred from expression pattern. Source: SGD protein amino acid phosphorylation Ref.3 Inferred from direct assay. Source: SGD response to DNA damage stimulus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 676	676	Serine/threonine-protein kinase Haspin homolog ALK2		PRO_0000202464
Regions
	Domain	383 – 672	290	Protein kinase
	Nucleotide binding	389 – 397	9	ATP By similarity
	Motif	116 – 118	3	KEN box
	Motif	150 – 158	9	D box
	Compositional bias	29 – 309	281	Ser-rich
Sites
	Binding site	430	1	ATP By similarity
Experimental info
	Mutagenesis	528 – 532	5	EHRNL → AAAAA: Reduces strongly kinase activity. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P32789-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 0E7B84FC74142206	FASTA	676	76,372
	10 20 30 40 50 60 MNFDAVADQQ MTDRRYFALE VAESDDADSS LNSSSMGSPA VDVGRKVYKI TSHKGSAEDE 70 80 90 100 110 120 SQSFFTSSDS PTSKTRPVGK TIENDDYYGK RSSTGSSLKQ LFNKININDT AHSSNKENVS 130 140 150 160 170 180 QSVLSENKLL SPSKRLSKQG LTKVTNSKFR TPLRPISNQS TLSRDEPVKD FRSLKFRSGS 190 200 210 220 230 240 DFKCWGDEKT SSHVHSSSVN SVNSFTSTTS SSKWKFWKND NLLSRSLSSR SVNDQDPNFV 250 260 270 280 290 300 QPKPTNSLQK KSSISSFHNS IFGGGKHTEK KRNSGFIMPD HQSTKELNHK HSSSNLSFRS 310 320 330 340 350 360 LKHKTSHSSL NKLKVRRKGN TQELNHPIKK TCQISLPVPD QVSKDKIQLK LKNSTSLASL 370 380 390 400 410 420 SSEVTPINTL DYNDSILQQI LQLCDVKYIL HDLREAQSLG LFTLNTRSVQ LSHNFWQTYH 430 440 450 460 470 480 SDMQTSLICK KVCLGALSDL TTSNLISLHE LKSLRLIQGT SGVANLLQAY VVPSNQCEND 490 500 510 520 530 540 QNLILYLFFK YQGTPLSRCS NIDYSQALSI FWQCSSILYV AESKFQLEHR NLTLDHILID 550 560 570 580 590 600 SKGNVTLIDM KCCRFLNIDN NKASYTRLDH HYFFQGRGTL QFEIYELMRS MLPQPISWAT 610 620 630 640 650 660 FEPRTNLLWL YHLSSSLLKM AKKAVVSGAL NREENILIEL THLLDPARKH SKTIFKKELV 670 IRTCGDLLSL KGEIMQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of a 12.7 kb segment of yeast chromosome II identifies a PDR-like gene and several new open reading frames." Delaveau T., Jacq C., Perea J. Yeast 8:761-768(1992) [PubMed: 1441753] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Alk1 and Alk2 are two new cell cycle-regulated haspin-like proteins in budding yeast." Nespoli A., Vercillo R., di Nola L., Diani L., Giannattasio M., Plevani P., Muzi-Falconi M. Cell Cycle 5:1464-1471(2006) [PubMed: 16855400] [Abstract] Cited for: FUNCTION, INDUCTION, PHOSPHORYLATION, DOMAINS, MUTAGENESIS OF 528-E--L-532.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z35770 Genomic DNA. Translation: CAA84828.1. S47695 Genomic DNA. Translation: AAB23988.1.
PIR	S41217.
RefSeq	NP_009544.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5484N.
IntAct	P32789. 4 interactions.
Genome annotation databases
Ensembl	YBL009W; YBL009W; YBL009W; Saccharomyces cerevisiae. [Genome view]
GeneID	852274.
GenomeReviews	Gene locus YBL009W in contig Y13134_GR.
KEGG	sce:YBL009W.
NMPDR	fig|4932.3.peg.237.
Organism-specific databases
CYGD	YBL009w.
SGD	S000000105. ALK2.
Enzyme and pathway databases
BRENDA	2.7.11.1. 250.
Gene expression databases
ArrayExpress	P32789.
Genevestigator	P32789.
GermOnline	YBL009W. Saccharomyces cerevisiae.
Family and domain databases
PROSITE	PS50011. PROTEIN_KINASE_DOM. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	970887.

Entry information

Entry name

ALK2_YEAST

Accession

Primary (citable) accession number: P32789

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	October 13, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)