P32787 (MG101_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial genome maintenance protein MGM101

Gene names

Name:	MGM101
Synonyms:	MGM9
Ordered Locus Names:	YJR144W
ORF Names:	J2181

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Performs an essential function in the repair of oxidatively damaged mtDNA that is required for the maintenance of the mitochondrial genome. Binds to DNA. Ref.4
Subcellular location	Mitochondrion matrix › mitochondrion nucleoid Ref.4 Ref.5.
Sequence similarities	Belongs to the MGM101 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Mitochondrion Mitochondrion nucleoid
Domain	Transit peptide
Ligand	DNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mitochondrial genome maintenance Inferred from electronic annotation. Source: InterPro recombinational repair Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrial chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	DNA strand annealing activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RIM1	P32445	1	EBI-10299,EBI-15206

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 30	30	Mitochondrion Potential
	Chain	31 – 269	239	Mitochondrial genome maintenance protein MGM101		PRO_0000021719

Sequences

	Sequence		Length	Mass (Da)
	P32787 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: E14C17F2E837D6F0	FASTA	269	30,091
	10 20 30 40 50 60 MKSIFKVRGC VSHAAQFCQK RTVVSTGTSN TATAGAVRKS FNSTETKPVF ATKSEAGNGS 70 80 90 100 110 120 HMKEYSSGIN SKLGGTPLET RSTADDSLNN SYKQVKGDID WYTSWYGLGM KPFEAKVQKD 130 140 150 160 170 180 LIEPLDPKDI EIKPDGLIYL PEIKYRRILN KAFGAGGWGL VPRSQTIVTS KLVTREYGLI 190 200 210 220 230 240 CHGQLISVAR GEQDYFNEAG IPTATEGCKS NALMRCCKDL GVGSELWDPV FIKKFKVDHC 250 260 TEKFVEHVTT KRKKKIWLRK DRQVEYPYK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"MGM101, a nuclear gene involved in maintenance of the mitochondrial genome in Saccharomyces cerevisiae." Chen X.-J., Guan M.-X., Clark-Walker G.D. Nucleic Acids Res. 21:3473-3477(1993) [PubMed: 8346025] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Mgm101p is a novel component of the mitochondrial nucleoid that binds DNA and is required for the repair of oxidatively damaged mitochondrial DNA." Meeusen S., Tieu Q., Wong E., Weiss E., Schieltz D., Yates J.R. III, Nunnari J. J. Cell Biol. 145:291-304(1999) [PubMed: 10209025] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, MASS SPECTROMETRY.
[5]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed: 14576278] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X68482 Genomic DNA. Translation: CAA48502.1. Z49644 Genomic DNA. Translation: CAA89677.1. BK006943 Genomic DNA. Translation: DAA08929.1.
PIR	S34849.
RefSeq	NP_012678.1. NM_001181802.1.
3D structure databases
ProteinModelPortal	P32787.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5044N.
IntAct	P32787. 11 interactions.
MINT	MINT-511113.
STRING	P32787.
2D gel databases
UCD-2DPAGE	P32787.
Proteomic databases
PeptideAtlas	P32787.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJR144W; YJR144W; YJR144W.
GeneID	853609.
KEGG	sce:YJR144W.
NMPDR	fig|4932.3.peg.3655.
Organism-specific databases
CYGD	YJR144w.
SGD	S000003905. MGM101.
Phylogenomic databases
eggNOG	fuNOG05515.
GeneTree	EFGT00050000005714.
HOGENOM	HBG448424.
OMA	KLVTREY.
OrthoDB	EOG4D5672.
Gene expression databases
ArrayExpress	P32787.
Genevestigator	P32787.
GermOnline	YJR144W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR009446. Mgm101p. [Graphical view]
Pfam	PF06420. Mgm101p. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974451.

Entry information

Entry name

MG101_YEAST

Accession

Primary (citable) accession number: P32787
Secondary accession number(s): D6VWW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	January 25, 2012
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program