P32787 (MG101_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial genome maintenance protein MGM101

Gene names

Name:	MGM101
Synonyms:	MGM9
Ordered Locus Names:	YJR144W
ORF Names:	J2181

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Performs an essential function in the repair of oxidatively damaged mtDNA that is required for the maintenance of the mitochondrial genome. Binds to DNA. Ref.4
Subcellular location	Mitochondrion matrix › mitochondrion nucleoid Ref.4 Ref.5.
Sequence similarities	Belongs to the MGM101 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Mitochondrion Mitochondrion nucleoid
Domain	Transit peptide
Ligand	DNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA strand renaturation Inferred from direct assay PubMed 22027892. Source: SGD interstrand cross-link repair Inferred from genetic interaction PubMed 22912599. Source: SGD mitochondrial genome maintenance Inferred from electronic annotation. Source: InterPro recombinational repair Inferred from mutant phenotype PubMed 22027892. Source: SGD
Cellular_component	mitochondrial chromosome Inferred from electronic annotation. Source: InterPro mitochondrial nucleoid Inferred from direct assay PubMed 10869431. Source: SGD
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RIM1	P32445	1	EBI-10299,EBI-15206

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 30	30	Mitochondrion Potential
	Chain	31 – 269	239	Mitochondrial genome maintenance protein MGM101		PRO_0000021719

Sequences

	Sequence		Length	Mass (Da)
	P32787 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: E14C17F2E837D6F0	FASTA	269	30,091
	10 20 30 40 50 60 MKSIFKVRGC VSHAAQFCQK RTVVSTGTSN TATAGAVRKS FNSTETKPVF ATKSEAGNGS 70 80 90 100 110 120 HMKEYSSGIN SKLGGTPLET RSTADDSLNN SYKQVKGDID WYTSWYGLGM KPFEAKVQKD 130 140 150 160 170 180 LIEPLDPKDI EIKPDGLIYL PEIKYRRILN KAFGAGGWGL VPRSQTIVTS KLVTREYGLI 190 200 210 220 230 240 CHGQLISVAR GEQDYFNEAG IPTATEGCKS NALMRCCKDL GVGSELWDPV FIKKFKVDHC 250 260 TEKFVEHVTT KRKKKIWLRK DRQVEYPYK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"MGM101, a nuclear gene involved in maintenance of the mitochondrial genome in Saccharomyces cerevisiae." Chen X.-J., Guan M.-X., Clark-Walker G.D. Nucleic Acids Res. 21:3473-3477(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Mgm101p is a novel component of the mitochondrial nucleoid that binds DNA and is required for the repair of oxidatively damaged mitochondrial DNA." Meeusen S., Tieu Q., Wong E., Weiss E., Schieltz D., Yates J.R. III, Nunnari J. J. Cell Biol. 145:291-304(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, MASS SPECTROMETRY.
[5]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X68482 Genomic DNA. Translation: CAA48502.1. Z49644 Genomic DNA. Translation: CAA89677.1. BK006943 Genomic DNA. Translation: DAA08929.1.
PIR	S34849.
RefSeq	NP_012678.1. NM_001181802.1.
3D structure databases
ProteinModelPortal	P32787.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5044N.
IntAct	P32787. 11 interactions.
MINT	MINT-511113.
STRING	4932.YJR144W.
2D gel databases
UCD-2DPAGE	P32787.
Proteomic databases
PaxDb	P32787.
PeptideAtlas	P32787.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJR144W; YJR144W; YJR144W.
GeneID	853609.
KEGG	sce:YJR144W.
Organism-specific databases
CYGD	YJR144w.
SGD	S000003905. MGM101.
Phylogenomic databases
eggNOG	NOG80257.
HOGENOM	HOG000214596.
OMA	KLVTREY.
OrthoDB	EOG4D5672.
Enzyme and pathway databases
BioCyc	YEAST:G3O-31758-MONOMER.
Gene expression databases
Genevestigator	P32787.
GermOnline	YJR144W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR009446. Mgm101p. [Graphical view]
Pfam	PF06420. Mgm101p. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974451.

Entry information

Entry name

MG101_YEAST

Accession

Primary (citable) accession number: P32787
Secondary accession number(s): D6VWW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	May 29, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program