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P32785 (FMT_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methionyl-tRNA formyltransferase, mitochondrial

Short name=MtFMT
EC=2.1.2.9
Gene names
Name:FMT1
Ordered Locus Names:YBL013W
ORF Names:YBL0311, YBL0313
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length401 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Formylates methionyl-tRNA in mitochondria. Ref.5

Catalytic activity

10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet).

Subcellular location

Mitochondrion Ref.6 Ref.8.

Miscellaneous

Formylation of the initiator Met-tRNA is not essential for mitochondrial protein synthesis in S.cerevisiae.

Present with 1070 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the Fmt family.

Sequence caution

The sequence CAA84832.1 differs from that shown. Reason: Frameshift at position 387.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

TDH3P003591EBI-7033,EBI-7218

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2626Mitochondrion Potential
Chain27 – 401375Methionyl-tRNA formyltransferase, mitochondrial
PRO_0000010096

Regions

Region159 – 1624Tetrahydrofolate (THF) binding By similarity

Sequences

Sequence LengthMass (Da)Tools
P32785 [UniParc].

Last modified February 21, 2006. Version 2.
Checksum: 95E1C61C4E6D3AE1

FASTA40144,617
        10         20         30         40         50         60 
MVKMRRITPT RLLFTCRYIS NNASPPVQPL NVLFFGSDTF SNFSLQALNE LRQNNGSCGI 

        70         80         90        100        110        120 
VDNIQVVTRS PKWCGRQKSI LKYPPIFDMA EKLQLPRPIT CDTKQEMLAL SKLTPSRQGN 

       130        140        150        160        170        180 
PENDGSGAPF NAIIAVSFGK LIPGDLIRAV PLALNVHPSL LPRHKGSAPI QRALLEGDTY 

       190        200        210        220        230        240 
TGVTIQTLHP DRFDHGAIVA QTEPLAIATM LSKGRVNDST ADFNSEGLPR RTAILMDQLG 

       250        260        270        280        290        300 
ALGAQLLGQT LRERLYLPQN RVQAPTAYKP SYAHRITTED KRIHWARDSA AELLNKLETL 

       310        320        330        340        350        360 
GPLHAFKEAT AARKDAQNSV LKRILFHECK VMRDARLDNG SKPGMFKYDD IKDCILVTCR 

       370        380        390        400 
GNLLLCVSRL QFEGFAVERA GQFMARLRKR CGALSEKLVF L 

« Hide

References

« Hide 'large scale' references
[1]"Yeast mitochondrial translation initiation."
Williams E.H., Butler C.A., Fox T.D.
Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 24657 / D273-10B and ATCC 90840 / EAY235 / FY23.
[2]"The sequence of an 8 kb segment on the left arm of chromosome II from Saccharomyces cerevisiae identifies five new open reading frames of unknown functions, two tRNA genes and two transposable elements."
Skala J., van Dyck L., Purnelle B., Goffeau A.
Yeast 8:777-785(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Initiation of protein synthesis in Saccharomyces cerevisiae mitochondria without formylation of the initiator tRNA."
Li Y., Holmes W.B., Appling D.R., RajBhandary U.L.
J. Bacteriol. 182:2886-2892(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"The proteome of Saccharomyces cerevisiae mitochondria."
Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C.
Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: ATCC 76625 / YPH499.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY490279 Genomic DNA. Translation: AAR86694.1.
AY492339 Genomic DNA. Translation: AAR86695.1.
Z35774 Genomic DNA. Translation: CAA84832.1. Frameshift.
BK006936 Genomic DNA. Translation: DAA07107.1.
PIRS25331.
RefSeqNP_009540.2. NM_001178253.1.

3D structure databases

ProteinModelPortalP32785.
SMRP32785. Positions 28-395.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32687. 55 interactions.
DIPDIP-8221N.
IntActP32785. 3 interactions.
MINTMINT-4476015.
STRING4932.YBL013W.

Proteomic databases

PaxDbP32785.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBL013W; YBL013W; YBL013W.
GeneID852270.
KEGGsce:YBL013W.

Organism-specific databases

CYGDYBL013w.
SGDS000000109. FMT1.

Phylogenomic databases

eggNOGCOG0223.
GeneTreeENSGT00390000017828.
HOGENOMHOG000111151.
KOK00604.
OMAIKPTGRN.
OrthoDBEOG7V1G2Z.

Enzyme and pathway databases

BioCycYEAST:G3O-28918-MONOMER.
BRENDA2.1.2.9. 984.

Gene expression databases

GenevestigatorP32785.

Family and domain databases

Gene3D3.40.50.170. 1 hit.
InterProIPR005794. Fmt.
IPR005793. Formyl_trans_C.
IPR002376. Formyl_transf_N.
IPR015518. Met_tRNA_Form_TA-like.
[Graphical view]
PANTHERPTHR11138. PTHR11138. 1 hit.
PfamPF02911. Formyl_trans_C. 1 hit.
PF00551. Formyl_trans_N. 1 hit.
[Graphical view]
SUPFAMSSF53328. SSF53328. 1 hit.
TIGRFAMsTIGR00460. fmt. 1 hit.
ProtoNetSearch...

Other

NextBio970878.
PROP32785.

Entry information

Entry nameFMT_YEAST
AccessionPrimary (citable) accession number: P32785
Secondary accession number(s): D6VPY7, Q6RUA6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 21, 2006
Last modified: April 16, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families