P32785 (FMT_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionyl-tRNA formyltransferase, mitochondrial
Short name=MtFMT
EC=2.1.2.9

Gene names

Name:	FMT1
Ordered Locus Names:	YBL013W
ORF Names:	YBL0313, YBL0311

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Formylates methionyl-tRNA in mitochondria. Ref.5
Catalytic activity	10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) + H2O = tetrahydrofolate + N-formylmethionyl-tRNA(fMet).
Subcellular location	Mitochondrion Ref.6 Ref.8.
Miscellaneous	Formylation of the initiator Met-tRNA is not essential for mitochondrial protein synthesis in S.cerevisiae. Present with 1070 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the fmt family.
Sequence caution	The sequence CAA84832.1 differs from that shown. Reason: Frameshift at position 387.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from direct assay Ref.5. Source: SGD
Cellular component	mitochondrion Inferred from direct assay Ref.8. Source: SGD
Molecular function	methionyl-tRNA formyltransferase activity Inferred from direct assay Ref.5. Source: SGD methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TDH3	P00359	1	EBI-7033,EBI-7218

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 26	26	Mitochondrion Potential
	Chain	27 – 401	375	Methionyl-tRNA formyltransferase, mitochondrial		PRO_0000010096
Regions
	Region	159 – 162	4	Tetrahydrofolate (THF) binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	P32785 [UniParc]. Last modified February 21, 2006. Version 2. Checksum: 95E1C61C4E6D3AE1	FASTA	401	44,617
	10 20 30 40 50 60 MVKMRRITPT RLLFTCRYIS NNASPPVQPL NVLFFGSDTF SNFSLQALNE LRQNNGSCGI 70 80 90 100 110 120 VDNIQVVTRS PKWCGRQKSI LKYPPIFDMA EKLQLPRPIT CDTKQEMLAL SKLTPSRQGN 130 140 150 160 170 180 PENDGSGAPF NAIIAVSFGK LIPGDLIRAV PLALNVHPSL LPRHKGSAPI QRALLEGDTY 190 200 210 220 230 240 TGVTIQTLHP DRFDHGAIVA QTEPLAIATM LSKGRVNDST ADFNSEGLPR RTAILMDQLG 250 260 270 280 290 300 ALGAQLLGQT LRERLYLPQN RVQAPTAYKP SYAHRITTED KRIHWARDSA AELLNKLETL 310 320 330 340 350 360 GPLHAFKEAT AARKDAQNSV LKRILFHECK VMRDARLDNG SKPGMFKYDD IKDCILVTCR 370 380 390 400 GNLLLCVSRL QFEGFAVERA GQFMARLRKR CGALSEKLVF L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast mitochondrial translation initiation." Williams E.H., Butler C.A., Fox T.D. Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 24657 / D273-10B and ATCC 90840 / EAY235 / FY23.
[2]	"The sequence of an 8 kb segment on the left arm of chromosome II from Saccharomyces cerevisiae identifies five new open reading frames of unknown functions, two tRNA genes and two transposable elements." Skala J., van Dyck L., Purnelle B., Goffeau A. Yeast 8:777-785(1992) [PubMed: 1332308] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Initiation of protein synthesis in Saccharomyces cerevisiae mitochondria without formylation of the initiator tRNA." Li Y., Holmes W.B., Appling D.R., RajBhandary U.L. J. Bacteriol. 182:2886-2892(2000) [PubMed: 10781559] [Abstract] Cited for: FUNCTION.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed: 14576278] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY490279 Genomic DNA. Translation: AAR86694.1. AY492339 Genomic DNA. Translation: AAR86695.1. Z35774 Genomic DNA. Translation: CAA84832.1. Frameshift. BK006936 Genomic DNA. Translation: DAA07107.1.
PIR	S25331.
RefSeq	NP_009540.2. NM_001178253.1.
3D structure databases
ProteinModelPortal	P32785.
SMR	P32785. Positions 27-396.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-8221N.
IntAct	P32785. 3 interactions.
STRING	P32785.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBL013W; YBL013W; YBL013W.
GeneID	852270.
KEGG	sce:YBL013W.
NMPDR	fig|4932.3.peg.233.
Organism-specific databases
CYGD	YBL013w.
SGD	S000000109. FMT1.
Phylogenomic databases
eggNOG	fuNOG09641.
GeneTree	EFGT00050000005149.
HOGENOM	HBG272957.
OMA	KYSGSSP.
OrthoDB	EOG4KD9W4.
Enzyme and pathway databases
BRENDA	2.1.2.9. 984.
Gene expression databases
ArrayExpress	P32785.
Genevestigator	P32785.
GermOnline	YBL013W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005794. Fmt. IPR005793. Formyl_trans_C. IPR002376. Formyl_transf_N. IPR015518. Met_tRNA_Form_TA-like. [Graphical view]
Gene3D	G3DSA:3.40.50.170. Formyl_transf_N. 1 hit.
KO	K00604.
PANTHER	PTHR11138. Met_tRNA_Form_TA-like. 1 hit.
Pfam	PF02911. Formyl_trans_C. 1 hit. PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00460. Fmt. 1 hit.
ProtoNet	Search...
Other
NextBio	970878.

Entry information

Entry name

FMT_YEAST

Accession

Primary (citable) accession number: P32785
Secondary accession number(s): D6VPY7, Q6RUA6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	February 21, 2006
Last modified:	December 14, 2011
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program