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P32785

- FMT_YEAST

UniProt

P32785 - FMT_YEAST

Protein

Methionyl-tRNA formyltransferase, mitochondrial

Gene

FMT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 2 (21 Feb 2006)
      Previous versions | rss
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    Functioni

    Formylates methionyl-tRNA in mitochondria.1 Publication

    Catalytic activityi

    10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet).

    GO - Molecular functioni

    1. methionyl-tRNA formyltransferase activity Source: SGD

    GO - Biological processi

    1. conversion of methionyl-tRNA to N-formyl-methionyl-tRNA Source: SGD
    2. translational initiation Source: GOC

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Protein biosynthesis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28918-MONOMER.
    BRENDAi2.1.2.9. 984.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methionyl-tRNA formyltransferase, mitochondrial (EC:2.1.2.9)
    Short name:
    MtFMT
    Gene namesi
    Name:FMT1
    Ordered Locus Names:YBL013W
    ORF Names:YBL0311, YBL0313
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBL013w.
    SGDiS000000109. FMT1.

    Subcellular locationi

    Mitochondrion 2 Publications

    GO - Cellular componenti

    1. mitochondrion Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2626MitochondrionSequence AnalysisAdd
    BLAST
    Chaini27 – 401375Methionyl-tRNA formyltransferase, mitochondrialPRO_0000010096Add
    BLAST

    Proteomic databases

    PaxDbiP32785.

    Expressioni

    Gene expression databases

    GenevestigatoriP32785.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    TDH3P003591EBI-7033,EBI-7218

    Protein-protein interaction databases

    BioGridi32687. 55 interactions.
    DIPiDIP-8221N.
    IntActiP32785. 3 interactions.
    MINTiMINT-4476015.
    STRINGi4932.YBL013W.

    Structurei

    3D structure databases

    ProteinModelPortaliP32785.
    SMRiP32785. Positions 30-302.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni159 – 1624Tetrahydrofolate (THF) bindingBy similarity

    Sequence similaritiesi

    Belongs to the Fmt family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0223.
    GeneTreeiENSGT00390000017828.
    HOGENOMiHOG000111151.
    KOiK00604.
    OMAiIKPTGRN.
    OrthoDBiEOG7V1G2Z.

    Family and domain databases

    Gene3Di3.40.50.170. 1 hit.
    InterProiIPR005794. Fmt.
    IPR005793. Formyl_trans_C.
    IPR002376. Formyl_transf_N.
    IPR015518. Met_tRNA_Form_TA-like.
    [Graphical view]
    PANTHERiPTHR11138. PTHR11138. 1 hit.
    PfamiPF02911. Formyl_trans_C. 1 hit.
    PF00551. Formyl_trans_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF53328. SSF53328. 1 hit.
    TIGRFAMsiTIGR00460. fmt. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P32785-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVKMRRITPT RLLFTCRYIS NNASPPVQPL NVLFFGSDTF SNFSLQALNE    50
    LRQNNGSCGI VDNIQVVTRS PKWCGRQKSI LKYPPIFDMA EKLQLPRPIT 100
    CDTKQEMLAL SKLTPSRQGN PENDGSGAPF NAIIAVSFGK LIPGDLIRAV 150
    PLALNVHPSL LPRHKGSAPI QRALLEGDTY TGVTIQTLHP DRFDHGAIVA 200
    QTEPLAIATM LSKGRVNDST ADFNSEGLPR RTAILMDQLG ALGAQLLGQT 250
    LRERLYLPQN RVQAPTAYKP SYAHRITTED KRIHWARDSA AELLNKLETL 300
    GPLHAFKEAT AARKDAQNSV LKRILFHECK VMRDARLDNG SKPGMFKYDD 350
    IKDCILVTCR GNLLLCVSRL QFEGFAVERA GQFMARLRKR CGALSEKLVF 400
    L 401
    Length:401
    Mass (Da):44,617
    Last modified:February 21, 2006 - v2
    Checksum:i95E1C61C4E6D3AE1
    GO

    Sequence cautioni

    The sequence CAA84832.1 differs from that shown. Reason: Frameshift at position 387.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY490279 Genomic DNA. Translation: AAR86694.1.
    AY492339 Genomic DNA. Translation: AAR86695.1.
    Z35774 Genomic DNA. Translation: CAA84832.1. Frameshift.
    BK006936 Genomic DNA. Translation: DAA07107.1.
    PIRiS25331.
    RefSeqiNP_009540.2. NM_001178253.1.

    Genome annotation databases

    EnsemblFungiiYBL013W; YBL013W; YBL013W.
    GeneIDi852270.
    KEGGisce:YBL013W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY490279 Genomic DNA. Translation: AAR86694.1 .
    AY492339 Genomic DNA. Translation: AAR86695.1 .
    Z35774 Genomic DNA. Translation: CAA84832.1 . Frameshift.
    BK006936 Genomic DNA. Translation: DAA07107.1 .
    PIRi S25331.
    RefSeqi NP_009540.2. NM_001178253.1.

    3D structure databases

    ProteinModelPortali P32785.
    SMRi P32785. Positions 30-302.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32687. 55 interactions.
    DIPi DIP-8221N.
    IntActi P32785. 3 interactions.
    MINTi MINT-4476015.
    STRINGi 4932.YBL013W.

    Proteomic databases

    PaxDbi P32785.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBL013W ; YBL013W ; YBL013W .
    GeneIDi 852270.
    KEGGi sce:YBL013W.

    Organism-specific databases

    CYGDi YBL013w.
    SGDi S000000109. FMT1.

    Phylogenomic databases

    eggNOGi COG0223.
    GeneTreei ENSGT00390000017828.
    HOGENOMi HOG000111151.
    KOi K00604.
    OMAi IKPTGRN.
    OrthoDBi EOG7V1G2Z.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28918-MONOMER.
    BRENDAi 2.1.2.9. 984.

    Miscellaneous databases

    NextBioi 970878.
    PROi P32785.

    Gene expression databases

    Genevestigatori P32785.

    Family and domain databases

    Gene3Di 3.40.50.170. 1 hit.
    InterProi IPR005794. Fmt.
    IPR005793. Formyl_trans_C.
    IPR002376. Formyl_transf_N.
    IPR015518. Met_tRNA_Form_TA-like.
    [Graphical view ]
    PANTHERi PTHR11138. PTHR11138. 1 hit.
    Pfami PF02911. Formyl_trans_C. 1 hit.
    PF00551. Formyl_trans_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53328. SSF53328. 1 hit.
    TIGRFAMsi TIGR00460. fmt. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Yeast mitochondrial translation initiation."
      Williams E.H., Butler C.A., Fox T.D.
      Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 24657 / D273-10B and ATCC 90840 / EAY235 / FY23.
    2. "The sequence of an 8 kb segment on the left arm of chromosome II from Saccharomyces cerevisiae identifies five new open reading frames of unknown functions, two tRNA genes and two transposable elements."
      Skala J., van Dyck L., Purnelle B., Goffeau A.
      Yeast 8:777-785(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Initiation of protein synthesis in Saccharomyces cerevisiae mitochondria without formylation of the initiator tRNA."
      Li Y., Holmes W.B., Appling D.R., RajBhandary U.L.
      J. Bacteriol. 182:2886-2892(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: ATCC 76625 / YPH499.

    Entry informationi

    Entry nameiFMT_YEAST
    AccessioniPrimary (citable) accession number: P32785
    Secondary accession number(s): D6VPY7, Q6RUA6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: February 21, 2006
    Last modified: October 1, 2014
    This is version 124 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Formylation of the initiator Met-tRNA is not essential for mitochondrial protein synthesis in S.cerevisiae.
    Present with 1070 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3