Glycerol-3-phosphate O-acyltransferase 1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P32784 (GPT1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 110. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate O-acyltransferase 1
Short name=G-3-P acyltransferase 1
EC=2.3.1.15

Alternative name(s):
Dihydroxyacetone phosphate acyltransferase 1
Short name=DHAP-AT 1
EC=2.3.1.42
Glycerol-3-phosphate / dihydroxyacetone phosphate acyltransferase 1
Suppressor of choline-transport mutants 1

Gene names

Name:	SCT1
Synonyms:	GAT2, GPT1
Ordered Locus Names:	YBL011W
ORF Names:	YBL0309, YBL0315

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	759 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	G-3-P/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase. Ref.2
Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. Acyl-CoA + glycerone phosphate = CoA + acylglycerone phosphate.
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.7.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Miscellaneous	Present with 1050 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Phospholipid biosynthesis Phospholipid metabolism
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Acyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	CDP-diacylglycerol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway phospholipid biosynthetic process Inferred from genetic interaction Ref.2. Source: SGD
Cellular_component	endoplasmic reticulum Inferred from direct assay Ref.7 PubMed 19525420. Source: SGD endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD
Molecular_function	glycerol-3-phosphate O-acyltransferase activity Inferred from direct assay Ref.2. Source: SGD glycerone-phosphate O-acyltransferase activity Inferred from direct assay Ref.2. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 759

759

Glycerol-3-phosphate O-acyltransferase 1

PRO_0000195257

Regions

Transmembrane

49 – 66

Helical; Potential

Transmembrane

123 – 139

Helical; Potential

Transmembrane

260 – 276

Helical; Potential

Transmembrane

440 – 463

Helical; Potential

Transmembrane

494 – 516

Helical; Potential

Transmembrane

524 – 545

Helical; Potential

Motif

414 – 419

HXXXXD motif

Compositional bias

736 – 753

Poly-Glu

Amino acid modifications

Modified residue

641

Phosphoserine Ref.9

Experimental info

Sequence conflict

F → S in BAA07409. Ref.1

Sequence conflict

F → S in CAC85390. Ref.2

Sequence conflict

30 – 38

Missing in CAC85390. Ref.2

Sequence conflict

A → R in BAA07409. Ref.1

Sequence conflict

125

P → A in BAA07409. Ref.1

Sequence conflict

278

K → R in CAC85390. Ref.2

Sequence conflict

324

P → S in CAC85390. Ref.2

Sequence conflict

574

D → N in CAC85390. Ref.2

Sequence conflict

730

G → S in BAA07409. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P32784 [UniParc].

Last modified August 31, 2004. Version 3.
Checksum: CCB0D11E8ED7D728
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FASTA

759

85,694

        10         20         30         40         50         60 
MPAPKLTEKF ASSKSTQKTT NYSSIEAKSV KTSADQAYIY QEPSATKKIL YSIATWLLYN 

        70         80         90        100        110        120 
IFHCFFREIR GRGSFKVPQQ GPVIFVAAPH ANQFVDPVIL MGEVKKSVNR RVSFLIAESS 

       130        140        150        160        170        180 
LKQPPIGFLA SFFMAIGVVR PQDNLKPAEG TIRVDPTDYK RVIGHDTHFL TDCMPKGLIG 

       190        200        210        220        230        240 
LPKSMGFGEI QSIESDTSLT LRKEFKMAKP EIKTALLTGT TYKYAAKVDQ SCVYHRVFEH 

       250        260        270        280        290        300 
LAHNNCIGIF PEGGSHDRTN LLPLKAGVAI MALGCMDKHP DVNVKIVPCG MNYFHPHKFR 

       310        320        330        340        350        360 
SRAVVEFGDP IEIPKELVAK YHNPETNRDA VKELLDTISK GLQSVTVTCS DYETLMVVQT 

       370        380        390        400        410        420 
IRRLYMTQFS TKLPLPLIVE MNRRMVKGYE FYRNDPKIAD LTKDIMAYNA ALRHYNLPDH 

       430        440        450        460        470        480 
LVEEAKVNFA KNLGLVFFRS IGLCILFSLA MPGIIMFSPV FILAKRISQE KARTALSKST 

       490        500        510        520        530        540 
VKIKANDVIA TWKILIGMGF APLLYIFWSV LITYYLRHKP WNKIYVFSGS YISCVIVTYS 

       550        560        570        580        590        600 
ALIVGDIGMD GFKSLRPLVL SLTSPKGLQK LQKDRRNLAE RIIEVVNNFG SELFPDFDSA 

       610        620        630        640        650        660 
ALREEFDVID EEEEDRKTSE LNRRKMLRKQ KIKRQEKDSS SPIISQRDNH DAYEHHNQDS 

       670        680        690        700        710        720 
DGVSLVNSDN SLSNIPLFSS TFHRKSESSL ASTSVAPSSS SEFEVENEIL EEKNGLASKI 

       730        740        750 
AQAVLNKRIG ENTAREEEEE EEEEEEEEEE EEEGKEGDA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of a SCT1 gene which can suppress a choline-transport mutant of Saccharomyces cerevisiae." Matsushita M., Nikawa J. J. Biochem. 117:447-451(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The initial step of the glycerolipid pathway: identification of glycerol-3-phosphate / dihydroxyacetone phosphate dual substrate acyltransferases in Saccharomyces cerevisiae." Zheng Z., Zou J. J. Biol. Chem. 276:41710-41716(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: ATCC 204660 / DBY746.
[3]	"The sequence of an 8 kb segment on the left arm of chromosome II from Saccharomyces cerevisiae identifies five new open reading frames of unknown functions, two tRNA genes and two transposable elements." Skala J., van Dyck L., Purnelle B., Goffeau A. Yeast 8:777-785(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Sequence of a 12.7 kb segment of yeast chromosome II identifies a PDR-like gene and several new open reading frames." Delaveau T., Jacq C., Perea J. Yeast 8:761-768(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 609-759. Strain: ATCC 204508 / S288c.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-641, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D38256 Genomic DNA. Translation: BAA07409.1. AJ314608 Genomic DNA. Translation: CAC85390.1. Z35773 Genomic DNA. Translation: CAA84831.1. S47695 Genomic DNA. Translation: AAB23987.1. BK006936 Genomic DNA. Translation: DAA07108.1.
PIR	S25330.
RefSeq	NP_009542.1. NM_001178251.1.
3D structure databases
ProteinModelPortal	P32784.
ModBase	Search...
Protein-protein interaction databases
IntAct	P32784. 1 interaction.
MINT	MINT-4475988.
STRING	4932.YBL011W.
Proteomic databases
PaxDb	P32784.
PeptideAtlas	P32784.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBL011W; YBL011W; YBL011W.
GeneID	852271.
KEGG	sce:YBL011W.
Organism-specific databases
CYGD	YBL011w.
SGD	S000000107. SCT1.
Phylogenomic databases
eggNOG	COG0204.
GeneTree	ENSGT00530000067105.
HOGENOM	HOG000191288.
KO	K13507.
OMA	PCGMNYF.
OrthoDB	EOG4W6S49.
Enzyme and pathway databases
BRENDA	2.3.1.15. 984.
SABIO-RK	P32784.
UniPathway	UPA00557; UER00612.
Gene expression databases
Genevestigator	P32784.
GermOnline	YBL011W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002123. Plipid/glycerol_acylTrfase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	970881.

Entry information

Entry name

GPT1_YEAST

Accession

Primary (citable) accession number: P32784
Secondary accession number(s): D6VPY8, Q07062, Q96TV1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	August 31, 2004
Last modified:	May 1, 2013
This is version 110 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents