P32784 (GPT1_YEAST) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 110.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Glycerol-3-phosphate O-acyltransferase 1 Short name=G-3-P acyltransferase 1 EC=2.3.1.15 Alternative name(s): Dihydroxyacetone phosphate acyltransferase 1 Short name=DHAP-AT 1 EC=2.3.1.42 Glycerol-3-phosphate / dihydroxyacetone phosphate acyltransferase 1 Suppressor of choline-transport mutants 1 | ||||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome] | ||||||||
| Taxonomic identifier | 559292 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › ![]() |
Protein attributes
| Sequence length | 759 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | G-3-P/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase. Ref.2 |
| Catalytic activity | Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. Acyl-CoA + glycerone phosphate = CoA + acylglycerone phosphate. |
| Pathway | |
| Subcellular location | Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.7. |
| Domain | The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity. |
| Miscellaneous | Present with 1050 molecules/cell in log phase SD medium. |
| Sequence similarities | Belongs to the GPAT/DAPAT family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 759 | 759 | Glycerol-3-phosphate O-acyltransferase 1 | PRO_0000195257 | |||||
Regions | |||||||||
| Transmembrane | 49 – 66 | 18 | Helical; Potential | ||||||
| Transmembrane | 123 – 139 | 17 | Helical; Potential | ||||||
| Transmembrane | 260 – 276 | 17 | Helical; Potential | ||||||
| Transmembrane | 440 – 463 | 24 | Helical; Potential | ||||||
| Transmembrane | 494 – 516 | 23 | Helical; Potential | ||||||
| Transmembrane | 524 – 545 | 22 | Helical; Potential | ||||||
| Motif | 414 – 419 | 6 | HXXXXD motif | ||||||
| Compositional bias | 736 – 753 | 18 | Poly-Glu | ||||||
Amino acid modifications | |||||||||
| Modified residue | 641 | 1 | Phosphoserine Ref.9 | ||||||
Experimental info | |||||||||
| Sequence conflict | 10 | 1 | F → S in BAA07409. Ref.1 | ||||||
| Sequence conflict | 10 | 1 | F → S in CAC85390. Ref.2 | ||||||
| Sequence conflict | 30 – 38 | 9 | Missing in CAC85390. Ref.2 | ||||||
| Sequence conflict | 88 | 1 | A → R in BAA07409. Ref.1 | ||||||
| Sequence conflict | 125 | 1 | P → A in BAA07409. Ref.1 | ||||||
| Sequence conflict | 278 | 1 | K → R in CAC85390. Ref.2 | ||||||
| Sequence conflict | 324 | 1 | P → S in CAC85390. Ref.2 | ||||||
| Sequence conflict | 574 | 1 | D → N in CAC85390. Ref.2 | ||||||
| Sequence conflict | 730 | 1 | G → S in BAA07409. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Isolation and characterization of a SCT1 gene which can suppress a choline-transport mutant of Saccharomyces cerevisiae." Matsushita M., Nikawa J. J. Biochem. 117:447-451(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "The initial step of the glycerolipid pathway: identification of glycerol-3-phosphate / dihydroxyacetone phosphate dual substrate acyltransferases in Saccharomyces cerevisiae." Zheng Z., Zou J. J. Biol. Chem. 276:41710-41716(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: ATCC 204660 / DBY746. |
| [3] | "The sequence of an 8 kb segment on the left arm of chromosome II from Saccharomyces cerevisiae identifies five new open reading frames of unknown functions, two tRNA genes and two transposable elements." Skala J., van Dyck L., Purnelle B., Goffeau A. Yeast 8:777-785(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [4] | "Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. Kleine K.EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [5] | Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c. |
| [6] | "Sequence of a 12.7 kb segment of yeast chromosome II identifies a PDR-like gene and several new open reading frames." Delaveau T., Jacq C., Perea J. Yeast 8:761-768(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 609-759. Strain: ATCC 204508 / S288c. |
| [7] | "Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
| [8] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [9] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-641, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | D38256 Genomic DNA. Translation: BAA07409.1. AJ314608 Genomic DNA. Translation: CAC85390.1. Z35773 Genomic DNA. Translation: CAA84831.1. S47695 Genomic DNA. Translation: AAB23987.1. BK006936 Genomic DNA. Translation: DAA07108.1. |
| PIR | S25330. |
| RefSeq | NP_009542.1. NM_001178251.1. |
3D structure databases | |
| ProteinModelPortal | P32784. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P32784. 1 interaction. |
| MINT | MINT-4475988. |
| STRING | 4932.YBL011W. |
Proteomic databases | |
| PaxDb | P32784. |
| PeptideAtlas | P32784. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblFungi | YBL011W; YBL011W; YBL011W. |
| GeneID | 852271. |
| KEGG | sce:YBL011W. |
Organism-specific databases | |
| CYGD | YBL011w. |
| SGD | S000000107. SCT1. |
Phylogenomic databases | |
| eggNOG | COG0204. |
| GeneTree | ENSGT00530000067105. |
| HOGENOM | HOG000191288. |
| KO | K13507. |
| OMA | PCGMNYF. |
| OrthoDB | EOG4W6S49. |
Enzyme and pathway databases | |
| BRENDA | 2.3.1.15. 984. |
| SABIO-RK | P32784. |
| UniPathway | UPA00557; UER00612. |
Gene expression databases | |
| Genevestigator | P32784. |
| GermOnline | YBL011W. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR002123. Plipid/glycerol_acylTrfase. [Graphical view] |
| Pfam | PF01553. Acyltransferase. 1 hit. [Graphical view] |
| SMART | SM00563. PlsC. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 970881. |
Entry information
| Entry name | GPT1_YEAST | ||||||||
| Accession | Primary (citable) accession number: P32784 Secondary accession number(s): D6VPY8, Q07062, Q96TV1 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome II Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with
