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Protein

mRNA cap guanine-N7 methyltransferase

Gene

ABD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Responsible for methylating the 5'-cap structure of mRNAs.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.PROSITE-ProRule annotationBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei154S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei172S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation1
Sitei175mRNA cap bindingPROSITE-ProRule annotation1
Sitei181mRNA cap bindingPROSITE-ProRule annotation1
Binding sitei194S-adenosyl-L-methioninePROSITE-ProRule annotation1
Sitei206mRNA cap bindingPROSITE-ProRule annotation1
Binding sitei223S-adenosyl-L-methionine; via amide nitrogenBy similarity1
Binding sitei249S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei253mRNA capPROSITE-ProRule annotation1
Binding sitei254S-adenosyl-L-methionineBy similarity1
Binding sitei347mRNA capPROSITE-ProRule annotation1
Binding sitei416mRNA capPROSITE-ProRule annotation1

GO - Molecular functioni

  • mRNA (guanine-N7-)-methyltransferase activity Source: SGD
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • 7-methylguanosine mRNA capping Source: SGD

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase
Biological processmRNA capping, mRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:G3O-29167-MONOMER
YEAST:G3O-29167-MONOMER
BRENDAi2.1.1.56 984
ReactomeiR-SCE-72086 mRNA Capping
R-SCE-77075 RNA Pol II CTD phosphorylation and interaction with CE

Names & Taxonomyi

Protein namesi
Recommended name:
mRNA cap guanine-N7 methyltransferase (EC:2.1.1.56By similarity)
Alternative name(s):
mRNA (guanine-N(7)-)-methyltransferase
mRNA cap methyltransferase
Gene namesi
Name:ABD1
Ordered Locus Names:YBR236C
ORF Names:YBR1602
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR236C
SGDiS000000440 ABD1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi168V → A: Still viable; normal growth. 1 Publication1
Mutagenesisi170E → A: Non-viable; reduced enzyme activity to 8% of wild-type. 2 Publications1
Mutagenesisi170E → D: Still viable; increase in activity. 2 Publications1
Mutagenesisi170E → Q: Non-viable; reduced enzyme activity to 8% of wild-type. 2 Publications1
Mutagenesisi172G → A: Still viable; normal growth. 1 Publication1
Mutagenesisi174G → A: Non viable; no growth. 1 Publication1
Mutagenesisi176G → A: Still viable; normal growth. 1 Publication1
Mutagenesisi178D → A: Microcolony formation. 1 Publication1
Mutagenesisi181K → A: Still viable; normal growth. 1 Publication1
Mutagenesisi182Y → A: Still viable; normal growth. 1 Publication1
Mutagenesisi194D → A: Lethal; no enzyme activity. 2 Publications1
Mutagenesisi194D → E: Still viable; activity near to wild-type. 2 Publications1
Mutagenesisi194D → N: Lethal; no enzyme activity. 2 Publications1
Mutagenesisi206R → A: Lethal. 2 Publications1
Mutagenesisi206R → K: Still viable; little change in enzyme activity. 2 Publications1
Mutagenesisi253H → A: Still viable; normal growth. 1 Publication1
Mutagenesisi254Y → A: Non viable; no growth. 2 Publications1
Mutagenesisi254Y → F: Still viable; slow growth; near to wild-type enzyme activity. 2 Publications1
Mutagenesisi254Y → S: Lethal; Enzyme activity 10% of wild-type. 2 Publications1
Mutagenesisi276G → A: Still viable; normal growth. 1 Publication1
Mutagenesisi277G → A: Still viable; normal growth. 1 Publication1
Mutagenesisi282T → A: Still viable; normal growth. 1 Publication1
Mutagenesisi287E → A: Still viable; normal growth. 1 Publication1
Mutagenesisi347E → A: Still viable; normal growth. 1 Publication1
Mutagenesisi348Y → A: Still viable; normal growth. 1 Publication1
Mutagenesisi349V → A: Still viable; normal growth. 1 Publication1
Mutagenesisi350V → A: Still viable; normal growth. 1 Publication1
Mutagenesisi361E → A: Still viable; normal growth. 1 Publication1
Mutagenesisi362Y → A: Still viable; normal growth. 1 Publication1
Mutagenesisi363G → A: Still viable; normal growth. 1 Publication1
Mutagenesisi366L → A: Still viable; normal growth. 1 Publication1
Mutagenesisi367V → A: Still viable; normal growth. 1 Publication1
Mutagenesisi372F → A: Still viable; normal growth. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002101341 – 436mRNA cap guanine-N7 methyltransferaseAdd BLAST436

Proteomic databases

MaxQBiP32783
PaxDbiP32783
PRIDEiP32783

PTM databases

iPTMnetiP32783

Interactioni

Protein-protein interaction databases

BioGridi32931, 343 interactors
DIPiDIP-2503N
IntActiP32783, 10 interactors
MINTiP32783
STRINGi4932.YBR236C

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IC3model-A140-424[»]
ProteinModelPortaliP32783
SMRiP32783
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini101 – 430mRNA cap 0 methyltransferasePROSITE-ProRule annotationAdd BLAST330

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni150 – 151mRNA cap bindingPROSITE-ProRule annotation2

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0 methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000002368
HOGENOMiHOG000242614
InParanoidiP32783
KOiK00565
OMAiLNLVSCQ
OrthoDBiEOG092C2SMS

Family and domain databases

InterProiView protein in InterPro
IPR016899 mRNA_G-N7_MeTrfase
IPR004971 mRNA_G-N7_MeTrfase_dom
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF03291 Pox_MCEL, 1 hit
PIRSFiPIRSF028762 ABD1, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51562 RNA_CAP0_MT, 1 hit

Sequencei

Sequence statusi: Complete.

P32783-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTKPEKPIW MSQEDYDRQY GSITGDESST VSKKDSKVTA NAPGDGNGSL
60 70 80 90 100
PVLQSSSILT SKVSDLPIEA ESGFKIQKRR HERYDQEERL RKQRAQKLRE
110 120 130 140 150
EQLKRHEIEM TANRSINVDQ IVREHYNERT IIANRAKRNL SPIIKLRNFN
160 170 180 190 200
NAIKYMLIDK YTKPGDVVLE LGCGKGGDLR KYGAAGISQF IGIDISNASI
210 220 230 240 250
QEAHKRYRSM RNLDYQVVLI TGDCFGESLG VAVEPFPDCR FPCDIVSTQF
260 270 280 290 300
CLHYAFETEE KARRALLNVA KSLKIGGHFF GTIPDSEFIR YKLNKFPKEV
310 320 330 340 350
EKPSWGNSIY KVTFENNSYQ KNDYEFTSPY GQMYTYWLED AIDNVPEYVV
360 370 380 390 400
PFETLRSLAD EYGLELVSQM PFNKFFVQEI PKWIERFSPK MREGLQRSDG
410 420 430
RYGVEGDEKE AASYFYTMFA FRKVKQYIEP ESVKPN
Length:436
Mass (Da):50,341
Last modified:October 1, 1993 - v1
Checksum:iEA5A68F0C4A57C4C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti126Y → C in AAT92789 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12000 Genomic DNA Translation: AAA34383.1
Z36105 Genomic DNA Translation: CAA85199.1
AY692770 Genomic DNA Translation: AAT92789.1
BK006936 Genomic DNA Translation: DAA07352.1
PIRiS41782
RefSeqiNP_009795.3, NM_001178584.3

Genome annotation databases

EnsemblFungiiYBR236C; YBR236C; YBR236C
GeneIDi852538
KEGGisce:YBR236C

Similar proteinsi

Entry informationi

Entry nameiMCES_YEAST
AccessioniPrimary (citable) accession number: P32783
Secondary accession number(s): D6VQN2, Q6B2G0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 23, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

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