P32781 (AGA2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
A-agglutinin-binding subunit

Gene names

Name:	AGA2
Ordered Locus Names:	YGL032C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	87 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor binding subunit of the a-agglutinin heterodimer. S.cerevisiae a and alpha cells express the complementary cell surface glycoproteins a-agglutinin and alpha-agglutinin, respectively, which interact with one another to promote cellular aggregation during mating. Ref.1
Subunit structure	Heterodimer; disulfide-linked Probable. Interacts with SAG1. Ref.4 Ref.5
Miscellaneous	Present with 1160 molecules/cell in log phase SD medium. Ref.6

Ontologies

Keywords
Biological process	Cell adhesion
Domain	Signal
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	agglutination involved in conjugation with cellular fusion Traceable author statement Ref.4. Source: SGD
Cellular component	fungal-type cell wall Traceable author statement Ref.4. Source: SGD
Molecular function	cell adhesion molecule binding Traceable author statement Ref.5Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18
	Chain	19 – 87	69	A-agglutinin-binding subunit		PRO_0000020642
Amino acid modifications
	Glycosylation	22	1	O-linked (Man...)
	Glycosylation	30	1	O-linked (Man...)
	Glycosylation	32	1	O-linked (Man...)
	Glycosylation	39	1	O-linked (Man...)
	Glycosylation	63	1	O-linked (Man...)
	Glycosylation	66	1	O-linked (Man...)
	Glycosylation	75	1	O-linked (Man...)
	Disulfide bond	25		Interchain (with AGA1) Probable
	Disulfide bond	68		Interchain (with AGA1) Probable
Experimental info
	Mutagenesis	25	1	C → S: Abolishes agglutination; when associated with S-68. Ref.4
	Mutagenesis	68	1	C → S: Abolishes agglutination; when associated with S-25. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P32781 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: EA7DA943FAD743C3	FASTA	87	9,464
	10 20 30 40 50 60 MQLLRCFSIF SVIASVLAQE LTTICEQIPS PTLESTPYSL STTTILANGK AMQGVFEYYK 70 80 SVTFVSNCGS HPSTTSKGSP INTQYVF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Saccharomyces cerevisiae a- and alpha-agglutinin: characterization of their molecular interaction." Cappellaro C., Hauser K., Mrsa V., Watzele M., Watzele G., Gruber C., Tanner W. EMBO J. 10:4081-4088(1991) [PubMed: 1756718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Strain: ATCC 26786 / X2180-1A.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Mating type-specific cell-cell recognition of Saccharomyces cerevisiae: cell wall attachment and active sites of a- and alpha-agglutinin." Cappellaro C., Baldermann C., Rachel R., Tanner W. EMBO J. 13:4737-4744(1994) [PubMed: 7957044] [Abstract] Cited for: SUBUNIT, MUTAGENESIS OF CYS-25 AND CYS-68.
[5]	"Interaction of alpha-agglutinin and a-agglutinin, Saccharomyces cerevisiae sexual cell adhesion molecules." Zhao H., Shen Z.M., Kahn P.C., Lipke P.N. J. Bacteriol. 183:2874-2880(2001) [PubMed: 11292808] [Abstract] Cited for: INTERACTION WITH SAG1.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X62877 mRNA. Translation: CAA44670.1. Z72554 Genomic DNA. Translation: CAA96733.1. BK006941 Genomic DNA. Translation: DAA08067.1.
PIR	S18838.
RefSeq	NP_011483.1. NM_001180897.1.
3D structure databases
ProteinModelPortal	P32781.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5451N.
IntAct	P32781. 1 interaction.
MINT	MINT-559655.
STRING	P32781.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL032C; YGL032C; YGL032C.
GeneID	852851.
KEGG	sce:YGL032C.
NMPDR	fig|4932.3.peg.2590.
Organism-specific databases
CYGD	YGL032c.
SGD	S000003000. AGA2.
Phylogenomic databases
eggNOG	fuNOG15980.
OrthoDB	EOG4M3DKG.
Gene expression databases
ArrayExpress	P32781.
Genevestigator	P32781.
GermOnline	YGL032C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR014404. A-agglutinin. [Graphical view]
PIRSF	PIRSF002695. A-agglutinin. 1 hit.
ProDom	PD062190. A-agglutinin. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...
Other
NextBio	972443.

Entry information

Entry name

AGA2_YEAST

Accession

Primary (citable) accession number: P32781
Secondary accession number(s): D6VUA6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	December 14, 2011
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program