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P32776 (TFB1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA polymerase II transcription factor B subunit 1
Alternative name(s):
General transcription and DNA repair factor IIH subunit TFB1
Short name=TFIIH subunit TFB1
RNA polymerase II transcription factor B 73 kDa subunit
RNA polymerase II transcription factor B p73 subunit
Gene names
Name:TFB1
Ordered Locus Names:YDR311W
ORF Names:D9740.3
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length642 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as component of the general transcription and DNA repair factor IIH (TFIIH) core, which is essential for both basal and activated transcription, and is involved in nucleotide excision repair (NER) of damaged DNA. TFIIH has CTD kinase and DNA-dependent ATPase activity, and is essential for polymerase II transcription in vitro. Ref.5 Ref.6

Subunit structure

Component of the TFIIH core complex, which is composed of RAD3, SSL1, SSL2, TFB1, TFB2, TFB4 and TFB5. Ref.5 Ref.7

Subcellular location

Nucleus.

Miscellaneous

Present with 5150 molecules/cell in log phase SD medium.

Sequence similarities

Contains 2 BSD domains.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

TFA1P361003EBI-19146,EBI-18903

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 642642RNA polymerase II transcription factor B subunit 1
PRO_0000119261

Regions

Domain165 – 22157BSD 1
Domain243 – 29553BSD 2

Amino acid modifications

Modified residue1501Phosphothreonine Ref.9

Experimental info

Sequence conflict2091F → L in AAU09707. Ref.4

Secondary structure

........................ 642
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P32776 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: DAE369FE90A224F8

FASTA64272,894
        10         20         30         40         50         60 
MSHSGAAIFE KVSGIIAINE DVSPAELTWR STDGDKVHTV VLSTIDKLQA TPASSEKMML 

        70         80         90        100        110        120 
RLIGKVDESK KRKDNEGNEV VPKPQRHMFS FNNRTVMDNI KMTLQQIISR YKDADIYEEK 

       130        140        150        160        170        180 
RRREESAQHT ETPMSSSSVT AGTPTPHLDT PQLNNGAPLI NTAKLDDSLS KEKLLTNLKL 

       190        200        210        220        230        240 
QQSLLKGNKV LMKVFQETVI NAGLPPSEFW STRIPLLRAF ALSTSQKVGP YNVLSTIKPV 

       250        260        270        280        290        300 
ASSENKVNVN LSREKILNIF ENYPIVKKAY TDNVPKNFKE PEFWARFFSS KLFRKLRGEK 

       310        320        330        340        350        360 
IMQNDRGDVI IDRYLTLDQE FDRKDDDMLL HPVKKIIDLD GNIQDDPVVR GNRPDFTMQP 

       370        380        390        400        410        420 
GVDINGNSDG TVDILKGMNR LSEKMIMALK NEYSRTNLQN KSNITNDEED EDNDERNELK 

       430        440        450        460        470        480 
IDDLNESYKT NYAIIHLKRN AHEKTTDNDA KSSADSIKNA DLKVSNQQML QQLSLVMDNL 

       490        500        510        520        530        540 
INKLDLNQVV PNNEVSNKIN KRVITAIKIN AKQAKHNNVN SALGSFVDNT SQANELEVKS 

       550        560        570        580        590        600 
TLPIDLLESC RMLHTTCCEF LKHFYIHFQS GEQKQASTVK KLYNHLKDCI EKLNELFQDV 

       610        620        630        640 
LNGDGESMSN TCTAYLKPVL NSITLATHKY DEYFNEYNNN SN 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of a subunit of yeast RNA polymerase II transcription factor b and CTD kinase."
Gileadi O., Feaver W.J., Kornberg R.D.
Science 257:1389-1392(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 314-322 AND 630-639.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"RNA polymerase transcription factor IIH holoenzyme from yeast."
Svejstrup J.Q., Feaver W.J., LaPointe J., Kornberg R.D.
J. Biol. Chem. 269:28044-28048(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF TFIIH IN RNA POLYMERASE II TRANSCRIPTION, IDENTIFICATION IN THE TFIIH COMPLEX.
[6]"Reconstitution of TFIIH and requirement of its DNA helicase subunits, Rad3 and Rad25, in the incision step of nucleotide excision repair."
Sung P., Guzder S.N., Prakash L., Prakash S.
J. Biol. Chem. 271:10821-10826(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE TFIIH CORE COMPLEX IN DNA REPAIR.
[7]"Revised subunit structure of yeast transcription factor IIH (TFIIH) and reconciliation with human TFIIH."
Takagi Y., Komori H., Chang W.-H., Hudmon A., Erdjument-Bromage H., Tempst P., Kornberg R.D.
J. Biol. Chem. 278:43897-43900(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE TFIIH CORE COMPLEX.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-150, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M95750 Genomic DNA. Translation: AAA35143.1.
U28374 Genomic DNA. Translation: AAB64747.1.
AY723790 Genomic DNA. Translation: AAU09707.1.
BK006938 Genomic DNA. Translation: DAA12150.1.
PIRS31285.
RefSeqNP_010597.3. NM_001180619.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1Y5ONMR-A1-115[»]
2GS0NMR-A1-115[»]
2K2UNMR-A1-115[»]
2L2INMR-A2-115[»]
2LOXNMR-A2-115[»]
2M14NMR-A2-115[»]
2MKRNMR-A1-115[»]
ProteinModelPortalP32776.
SMRP32776. Positions 2-115.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32364. 73 interactions.
DIPDIP-1702N.
IntActP32776. 21 interactions.
MINTMINT-387816.
STRING4932.YDR311W.

Proteomic databases

MaxQBP32776.
PaxDbP32776.
PeptideAtlasP32776.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDR311W; YDR311W; YDR311W.
GeneID851906.
KEGGsce:YDR311W.

Organism-specific databases

CYGDYDR311w.
SGDS000002719. TFB1.

Phylogenomic databases

eggNOGNOG315835.
GeneTreeENSGT00390000015066.
HOGENOMHOG000248691.
KOK03141.
OMAEFWARFF.
OrthoDBEOG7QRR3J.

Enzyme and pathway databases

BioCycYEAST:G3O-29870-MONOMER.

Gene expression databases

GenevestigatorP32776.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
InterProIPR005607. BSD.
IPR011993. PH_like_dom.
IPR027079. Tfb1/p62.
IPR013876. TFIIH_BTF_p62_N.
[Graphical view]
PANTHERPTHR12856. PTHR12856. 1 hit.
PfamPF03909. BSD. 2 hits.
PF08567. TFIIH_BTF_p62_N. 1 hit.
[Graphical view]
SMARTSM00751. BSD. 2 hits.
[Graphical view]
PROSITEPS50858. BSD. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP32776.
NextBio969921.
PROP32776.

Entry information

Entry nameTFB1_YEAST
AccessionPrimary (citable) accession number: P32776
Secondary accession number(s): D6VSU0, E9P948
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: July 9, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references