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Protein

RNA polymerase II transcription factor B subunit 1

Gene

TFB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as component of the general transcription and DNA repair factor IIH (TFIIH) core, which is essential for both basal and activated transcription, and is involved in nucleotide excision repair (NER) of damaged DNA. TFIIH has CTD kinase and DNA-dependent ATPase activity, and is essential for polymerase II transcription in vitro.2 Publications

GO - Molecular functioni

  • phosphatidylinositol-3-phosphate binding Source: SGD
  • phosphatidylinositol-5-phosphate binding Source: SGD

GO - Biological processi

  • nucleotide-excision repair Source: SGD
  • phosphorylation of RNA polymerase II C-terminal domain Source: SGD
  • transcription from RNA polymerase II promoter Source: SGD
  • transcription from RNA polymerase I promoter Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-29870-MONOMER.
ReactomeiR-SCE-113418. Formation of the Early Elongation Complex.
R-SCE-5696395. Formation of Incision Complex in GG-NER.
R-SCE-5696400. Dual Incision in GG-NER.
R-SCE-674695. RNA Polymerase II Pre-transcription Events.
R-SCE-6781823. Formation of TC-NER Pre-Incision Complex.
R-SCE-6782135. Dual incision in TC-NER.
R-SCE-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-SCE-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-SCE-72086. mRNA Capping.
R-SCE-73776. RNA Polymerase II Promoter Escape.
R-SCE-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SCE-75953. RNA Polymerase II Transcription Initiation.
R-SCE-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-SCE-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase II transcription factor B subunit 1
Alternative name(s):
General transcription and DNA repair factor IIH subunit TFB1
Short name:
TFIIH subunit TFB1
RNA polymerase II transcription factor B 73 kDa subunit
RNA polymerase II transcription factor B p73 subunit
Gene namesi
Name:TFB1
Ordered Locus Names:YDR311W
ORF Names:D9740.3
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR311W.
SGDiS000002719. TFB1.

Subcellular locationi

GO - Cellular componenti

  • core TFIIH complex Source: SGD
  • cytosol Source: SGD
  • holo TFIIH complex Source: SGD
  • nucleotide-excision repair factor 3 complex Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001192611 – 642RNA polymerase II transcription factor B subunit 1Add BLAST642

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei150PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32776.
PRIDEiP32776.

PTM databases

iPTMnetiP32776.

Interactioni

Subunit structurei

Component of the TFIIH core complex, which is composed of RAD3, SSL1, SSL2, TFB1, TFB2, TFB4 and TFB5.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TFA1P361003EBI-19146,EBI-18903

Protein-protein interaction databases

BioGridi32364. 75 interactors.
DIPiDIP-1702N.
IntActiP32776. 21 interactors.
MINTiMINT-387816.

Structurei

Secondary structure

1642
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 11Combined sources9
Beta strandi13 – 19Combined sources7
Beta strandi21 – 24Combined sources4
Beta strandi26 – 35Combined sources10
Beta strandi37 – 42Combined sources6
Beta strandi48 – 50Combined sources3
Beta strandi53 – 55Combined sources3
Beta strandi56 – 64Combined sources9
Beta strandi69 – 71Combined sources3
Beta strandi75 – 77Combined sources3
Beta strandi85 – 92Combined sources8
Helixi94 – 114Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Y5ONMR-A1-115[»]
2GS0NMR-A1-115[»]
2K2UNMR-A1-115[»]
2L2INMR-A2-115[»]
2LOXNMR-A2-115[»]
2M14NMR-A2-115[»]
2MKRNMR-A1-115[»]
2N0YNMR-A1-115[»]
2N22NMR-A2-115[»]
2N23NMR-A2-115[»]
ProteinModelPortaliP32776.
SMRiP32776.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32776.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini165 – 221BSD 1PROSITE-ProRule annotationAdd BLAST57
Domaini243 – 295BSD 2PROSITE-ProRule annotationAdd BLAST53

Sequence similaritiesi

Contains 2 BSD domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00390000015066.
HOGENOMiHOG000248691.
InParanoidiP32776.
KOiK03141.
OMAiWRSTDGD.
OrthoDBiEOG092C337H.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR005607. BSD_dom.
IPR011993. PH_dom-like.
IPR027079. Tfb1/p62.
IPR013876. TFIIH_BTF_p62_N.
[Graphical view]
PANTHERiPTHR12856. PTHR12856. 3 hits.
PfamiPF03909. BSD. 2 hits.
PF08567. PH_TFIIH. 1 hit.
[Graphical view]
SMARTiSM00751. BSD. 2 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS50858. BSD. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32776-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHSGAAIFE KVSGIIAINE DVSPAELTWR STDGDKVHTV VLSTIDKLQA
60 70 80 90 100
TPASSEKMML RLIGKVDESK KRKDNEGNEV VPKPQRHMFS FNNRTVMDNI
110 120 130 140 150
KMTLQQIISR YKDADIYEEK RRREESAQHT ETPMSSSSVT AGTPTPHLDT
160 170 180 190 200
PQLNNGAPLI NTAKLDDSLS KEKLLTNLKL QQSLLKGNKV LMKVFQETVI
210 220 230 240 250
NAGLPPSEFW STRIPLLRAF ALSTSQKVGP YNVLSTIKPV ASSENKVNVN
260 270 280 290 300
LSREKILNIF ENYPIVKKAY TDNVPKNFKE PEFWARFFSS KLFRKLRGEK
310 320 330 340 350
IMQNDRGDVI IDRYLTLDQE FDRKDDDMLL HPVKKIIDLD GNIQDDPVVR
360 370 380 390 400
GNRPDFTMQP GVDINGNSDG TVDILKGMNR LSEKMIMALK NEYSRTNLQN
410 420 430 440 450
KSNITNDEED EDNDERNELK IDDLNESYKT NYAIIHLKRN AHEKTTDNDA
460 470 480 490 500
KSSADSIKNA DLKVSNQQML QQLSLVMDNL INKLDLNQVV PNNEVSNKIN
510 520 530 540 550
KRVITAIKIN AKQAKHNNVN SALGSFVDNT SQANELEVKS TLPIDLLESC
560 570 580 590 600
RMLHTTCCEF LKHFYIHFQS GEQKQASTVK KLYNHLKDCI EKLNELFQDV
610 620 630 640
LNGDGESMSN TCTAYLKPVL NSITLATHKY DEYFNEYNNN SN
Length:642
Mass (Da):72,894
Last modified:October 1, 1993 - v1
Checksum:iDAE369FE90A224F8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti209F → L in AAU09707 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95750 Genomic DNA. Translation: AAA35143.1.
U28374 Genomic DNA. Translation: AAB64747.1.
AY723790 Genomic DNA. Translation: AAU09707.1.
BK006938 Genomic DNA. Translation: DAA12150.1.
PIRiS31285.
RefSeqiNP_010597.3. NM_001180619.3.

Genome annotation databases

EnsemblFungiiYDR311W; YDR311W; YDR311W.
GeneIDi851906.
KEGGisce:YDR311W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95750 Genomic DNA. Translation: AAA35143.1.
U28374 Genomic DNA. Translation: AAB64747.1.
AY723790 Genomic DNA. Translation: AAU09707.1.
BK006938 Genomic DNA. Translation: DAA12150.1.
PIRiS31285.
RefSeqiNP_010597.3. NM_001180619.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Y5ONMR-A1-115[»]
2GS0NMR-A1-115[»]
2K2UNMR-A1-115[»]
2L2INMR-A2-115[»]
2LOXNMR-A2-115[»]
2M14NMR-A2-115[»]
2MKRNMR-A1-115[»]
2N0YNMR-A1-115[»]
2N22NMR-A2-115[»]
2N23NMR-A2-115[»]
ProteinModelPortaliP32776.
SMRiP32776.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32364. 75 interactors.
DIPiDIP-1702N.
IntActiP32776. 21 interactors.
MINTiMINT-387816.

PTM databases

iPTMnetiP32776.

Proteomic databases

MaxQBiP32776.
PRIDEiP32776.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR311W; YDR311W; YDR311W.
GeneIDi851906.
KEGGisce:YDR311W.

Organism-specific databases

EuPathDBiFungiDB:YDR311W.
SGDiS000002719. TFB1.

Phylogenomic databases

GeneTreeiENSGT00390000015066.
HOGENOMiHOG000248691.
InParanoidiP32776.
KOiK03141.
OMAiWRSTDGD.
OrthoDBiEOG092C337H.

Enzyme and pathway databases

BioCyciYEAST:G3O-29870-MONOMER.
ReactomeiR-SCE-113418. Formation of the Early Elongation Complex.
R-SCE-5696395. Formation of Incision Complex in GG-NER.
R-SCE-5696400. Dual Incision in GG-NER.
R-SCE-674695. RNA Polymerase II Pre-transcription Events.
R-SCE-6781823. Formation of TC-NER Pre-Incision Complex.
R-SCE-6782135. Dual incision in TC-NER.
R-SCE-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-SCE-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-SCE-72086. mRNA Capping.
R-SCE-73776. RNA Polymerase II Promoter Escape.
R-SCE-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SCE-75953. RNA Polymerase II Transcription Initiation.
R-SCE-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-SCE-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Miscellaneous databases

EvolutionaryTraceiP32776.
PROiP32776.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR005607. BSD_dom.
IPR011993. PH_dom-like.
IPR027079. Tfb1/p62.
IPR013876. TFIIH_BTF_p62_N.
[Graphical view]
PANTHERiPTHR12856. PTHR12856. 3 hits.
PfamiPF03909. BSD. 2 hits.
PF08567. PH_TFIIH. 1 hit.
[Graphical view]
SMARTiSM00751. BSD. 2 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS50858. BSD. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTFB1_YEAST
AccessioniPrimary (citable) accession number: P32776
Secondary accession number(s): D6VSU0, E9P948
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 2, 2016
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5150 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.