P32775 (GLGB_YEAST) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 106.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: 1,4-alpha-glucan-branching enzyme EC=2.4.1.18 Alternative name(s): Glycogen-branching enzyme | ||||
| Gene names |
| ||||
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | ||||
| Taxonomic identifier | 559292 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 704 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Catalytic activity | Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. |
| Pathway | |
| Developmental stage | Expressed during the transition between the late exponential and stationary growth phases, coincident with maximal glycogen accumulation. |
| Miscellaneous | Present with 1230 molecules/cell in log phase SD medium. Ref.4 |
| Sequence similarities | Belongs to the glycosyl hydrolase 13 family. GlgB subfamily. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Glycogen biosynthesis |
| Molecular function | Glycosyltransferase Transferase |
| PTM | Phosphoprotein |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | glycogen biosynthetic process Inferred from mutant phenotype. Source: SGD |
| Cellular component | cytoplasm Inferred from direct assay. Source: SGD |
| Molecular function | 1,4-alpha-glucan branching enzyme activity Inferred from genetic interaction. Source: SGD cation bindingInferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compoundsInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 704 | 704 | 1,4-alpha-glucan-branching enzyme | PRO_0000188786 | |||||
Sites | |||||||||
| Active site | 356 | 1 | Nucleophile By similarity | ||||||
| Active site | 417 | 1 | Proton donor By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 190 | 1 | Phosphoserine Ref.5 | ||||||
Experimental info | |||||||||
| Sequence conflict | 564 | 1 | S → T in AAA34632. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Coordinate regulation of glycogen metabolism in the yeast Saccharomyces cerevisiae. Induction of glycogen branching enzyme." Thon V.J., Vigneron-Lesens C., Marianne-Pepin T., Montreuil J., Decq A., Rachez C., Ball S.G., Cannon J.F. J. Biol. Chem. 267:15224-15228(1992) [PubMed: 1634552] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W.Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [3] | Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c. |
| [4] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [5] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-190, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | M76739 Genomic DNA. Translation: AAA34632.1. U18530 Genomic DNA. Translation: AAB64488.1. BK006939 Genomic DNA. Translation: DAA07642.1. |
| PIR | S50448. |
| RefSeq | NP_010905.1. NM_001178826.1. |
3D structure databases | |
| ProteinModelPortal | P32775. |
| SMR | P32775. Positions 8-704. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-3873N. |
| IntAct | P32775. 10 interactions. |
| MINT | MINT-573503. |
| STRING | P32775. |
Protein family/group databases | |
| CAZy | CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13. |
Proteomic databases | |
| PeptideAtlas | P32775. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblFungi | YEL011W; YEL011W; YEL011W. |
| GeneID | 856705. |
| KEGG | sce:YEL011W. |
| NMPDR | fig|4932.3.peg.1961. |
Organism-specific databases | |
| CYGD | YEL011w. |
| SGD | S000000737. GLC3. |
Phylogenomic databases | |
| eggNOG | fuNOG05596. |
| GeneTree | EFGT00050000004871. |
| HOGENOM | HBG302308. |
| OMA | YEAHIGM. |
| OrthoDB | EOG47M562. |
Gene expression databases | |
| ArrayExpress | P32775. |
| Genevestigator | P32775. |
| GermOnline | YEL011W. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view] |
| Gene3D | G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit. |
| KO | K00700. |
| PANTHER | PTHR10357. Alpha_amylase. 1 hit. |
| Pfam | PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view] |
| PIRSF | PIRSF000463. GlgB. 1 hit. |
| SUPFAM | SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit. |
| ProtoNet | Search... |
Other | |
| NextBio | 982774. |
Entry information
| Entry name | GLGB_YEAST | ||||||||
| Accession | Primary (citable) accession number: P32775 Secondary accession number(s): D3DLN8 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| Recent format changes Overview of recent format changes |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome V Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names |
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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