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P32775

- GLGB_YEAST

UniProt

P32775 - GLGB_YEAST

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Protein

1,4-alpha-glucan-branching enzyme

Gene

GLC3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei356 – 3561NucleophileBy similarity
Active sitei417 – 4171Proton donorBy similarity

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: SGD
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Glycogen biosynthesis

Enzyme and pathway databases

BioCyciYEAST:YEL011W-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan-branching enzyme (EC:2.4.1.18)
Alternative name(s):
Glycogen-branching enzyme
Gene namesi
Name:GLC3
Ordered Locus Names:YEL011W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYEL011w.
SGDiS000000737. GLC3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7047041,4-alpha-glucan-branching enzymePRO_0000188786Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei190 – 1901Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32775.
PaxDbiP32775.
PeptideAtlasiP32775.

Expressioni

Developmental stagei

Expressed during the transition between the late exponential and stationary growth phases, coincident with maximal glycogen accumulation.

Gene expression databases

GenevestigatoriP32775.

Interactioni

Protein-protein interaction databases

BioGridi36720. 12 interactions.
DIPiDIP-3873N.
IntActiP32775. 10 interactions.
MINTiMINT-573503.
STRINGi4932.YEL011W.

Structurei

3D structure databases

ProteinModelPortaliP32775.
SMRiP32775. Positions 51-700.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0296.
GeneTreeiENSGT00390000017040.
HOGENOMiHOG000175159.
InParanoidiP32775.
KOiK00700.
OMAiNEVIHFL.
OrthoDBiEOG7647B5.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

P32775-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYNIPDNVKG AVEFDPWLKP FADVLSERRY LADKWLYDIT HATPDGSYQS
60 70 80 90 100
LSKFARDSYK SYGLHANPET KEITYKEWAP NAERAFLVGD FNNWDTTSHE
110 120 130 140 150
LKNKDEFGNF TITLHPLPNG DFAIPHDSKI KVMFILPDGS KIFRLPAWIT
160 170 180 190 200
RATQPSKETS KQFGPAYEGR FWNPENPYKF VHPRPKFSES VDSLRIYEAH
210 220 230 240 250
VGISSPEPKI TTYKEFTEKV LPRIKYLGYD AIQLMAIMEH AYYASFGYQV
260 270 280 290 300
TNFFAASSRF GTPEELKELI DTAHSMGILV LLDVVHSHAS KNVEDGLNMF
310 320 330 340 350
DGSDHQYFHS ISSGRGEHPL WDSRLFNYGK FEVQRFLLAN LAFYVDVYQF
360 370 380 390 400
DGFRFDGVTS MLYVHHGVGA GGSFSGDYNE YLSRDRSFVD HEALAYLMLA
410 420 430 440 450
NDLVHEMLPN LAVTVAEDVS GYPTLCLPRS IGGTGFDYRL AMALPDMWIK
460 470 480 490 500
LIKEKKDDEW EMGSIVYTLT NRRYGEKVVA YCESHDQALV GDKTLAFWLM
510 520 530 540 550
DAAMYTDMTV LKEPSIVIDR GIALHKMIRL ITHSLGGEAY LNFEGNEFGH
560 570 580 590 600
PEWLDFPNVN NGDSYKYARR QFNLADDPLL RYQNLNEFDR SMQLCEKRHK
610 620 630 640 650
WLNTKQAYVS LKHEGDKMIV FERNNLLFIF NFHPTNSYSD YRVGVEKAGT
660 670 680 690 700
YHIVLNSDRA EFGGHNRINE SSEFFTTDLE WNNRKNFLQV YIPSRVALVL

ALKE
Length:704
Mass (Da):81,116
Last modified:February 1, 1995 - v2
Checksum:i9C227E107B825F27
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti564 – 5641S → T in AAA34632. (PubMed:1634552)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M76739 Genomic DNA. Translation: AAA34632.1.
U18530 Genomic DNA. Translation: AAB64488.1.
BK006939 Genomic DNA. Translation: DAA07642.1.
PIRiS50448.
RefSeqiNP_010905.1. NM_001178826.1.

Genome annotation databases

EnsemblFungiiYEL011W; YEL011W; YEL011W.
GeneIDi856705.
KEGGisce:YEL011W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M76739 Genomic DNA. Translation: AAA34632.1 .
U18530 Genomic DNA. Translation: AAB64488.1 .
BK006939 Genomic DNA. Translation: DAA07642.1 .
PIRi S50448.
RefSeqi NP_010905.1. NM_001178826.1.

3D structure databases

ProteinModelPortali P32775.
SMRi P32775. Positions 51-700.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36720. 12 interactions.
DIPi DIP-3873N.
IntActi P32775. 10 interactions.
MINTi MINT-573503.
STRINGi 4932.YEL011W.

Protein family/group databases

CAZyi CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Proteomic databases

MaxQBi P32775.
PaxDbi P32775.
PeptideAtlasi P32775.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YEL011W ; YEL011W ; YEL011W .
GeneIDi 856705.
KEGGi sce:YEL011W.

Organism-specific databases

CYGDi YEL011w.
SGDi S000000737. GLC3.

Phylogenomic databases

eggNOGi COG0296.
GeneTreei ENSGT00390000017040.
HOGENOMi HOG000175159.
InParanoidi P32775.
KOi K00700.
OMAi NEVIHFL.
OrthoDBi EOG7647B5.

Enzyme and pathway databases

UniPathwayi UPA00164 .
BioCyci YEAST:YEL011W-MONOMER.

Miscellaneous databases

NextBioi 982774.
PROi P32775.

Gene expression databases

Genevestigatori P32775.

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view ]
PIRSFi PIRSF000463. GlgB. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Coordinate regulation of glycogen metabolism in the yeast Saccharomyces cerevisiae. Induction of glycogen branching enzyme."
    Thon V.J., Vigneron-Lesens C., Marianne-Pepin T., Montreuil J., Decq A., Rachez C., Ball S.G., Cannon J.F.
    J. Biol. Chem. 267:15224-15228(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-190, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGLGB_YEAST
AccessioniPrimary (citable) accession number: P32775
Secondary accession number(s): D3DLN8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1230 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3