Reviewed, UniProtKB/Swiss-Prot P32775 (GLGB_YEAST)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme

Gene names

Name:	GLC3
Ordered Locus Names:	YEL011W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	704 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.
Pathway	Glycan biosynthesis; glycogen biosynthesis.
Developmental stage	Expressed during the transition between the late exponential and stationary growth phases, coincident with maximal glycogen accumulation.
Miscellaneous	Present with 1230 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from genetic interaction. Source: SGD cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 704	704	1,4-alpha-glucan-branching enzyme		PRO_0000188786
Sites
	Active site	248	1	By similarity
	Active site	283	1	By similarity
	Active site	288	1	By similarity
	Active site	354	1	By similarity
	Active site	356	1	By similarity
	Active site	417	1	By similarity
	Active site	485	1	By similarity
	Active site	486	1	By similarity
Amino acid modifications
	Modified residue	190	1	Phosphoserine Ref.4
Experimental info
	Sequence conflict	564	1	S → T in AAA34632. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P32775-1 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: 9C227E107B825F27	FASTA	704	81,116
	10 20 30 40 50 60 MYNIPDNVKG AVEFDPWLKP FADVLSERRY LADKWLYDIT HATPDGSYQS LSKFARDSYK 70 80 90 100 110 120 SYGLHANPET KEITYKEWAP NAERAFLVGD FNNWDTTSHE LKNKDEFGNF TITLHPLPNG 130 140 150 160 170 180 DFAIPHDSKI KVMFILPDGS KIFRLPAWIT RATQPSKETS KQFGPAYEGR FWNPENPYKF 190 200 210 220 230 240 VHPRPKFSES VDSLRIYEAH VGISSPEPKI TTYKEFTEKV LPRIKYLGYD AIQLMAIMEH 250 260 270 280 290 300 AYYASFGYQV TNFFAASSRF GTPEELKELI DTAHSMGILV LLDVVHSHAS KNVEDGLNMF 310 320 330 340 350 360 DGSDHQYFHS ISSGRGEHPL WDSRLFNYGK FEVQRFLLAN LAFYVDVYQF DGFRFDGVTS 370 380 390 400 410 420 MLYVHHGVGA GGSFSGDYNE YLSRDRSFVD HEALAYLMLA NDLVHEMLPN LAVTVAEDVS 430 440 450 460 470 480 GYPTLCLPRS IGGTGFDYRL AMALPDMWIK LIKEKKDDEW EMGSIVYTLT NRRYGEKVVA 490 500 510 520 530 540 YCESHDQALV GDKTLAFWLM DAAMYTDMTV LKEPSIVIDR GIALHKMIRL ITHSLGGEAY 550 560 570 580 590 600 LNFEGNEFGH PEWLDFPNVN NGDSYKYARR QFNLADDPLL RYQNLNEFDR SMQLCEKRHK 610 620 630 640 650 660 WLNTKQAYVS LKHEGDKMIV FERNNLLFIF NFHPTNSYSD YRVGVEKAGT YHIVLNSDRA 670 680 690 700 EFGGHNRINE SSEFFTTDLE WNNRKNFLQV YIPSRVALVL ALKE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Coordinate regulation of glycogen metabolism in the yeast Saccharomyces cerevisiae. Induction of glycogen branching enzyme." Thon V.J., Vigneron-Lesens C., Marianne-Pepin T., Montreuil J., Decq A., Rachez C., Ball S.G., Cannon J.F. J. Biol. Chem. 267:15224-15228(1992) [PubMed: 1634552] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-190, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M76739 Genomic DNA. Translation: AAA34632.1. U18530 Genomic DNA. Translation: AAB64488.1.
PIR	S50448.
RefSeq	NP_010905.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3873N.
IntAct	P32775. 14 interactions.
STRING	P32775.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Proteomic databases
PeptideAtlas	P32775.
Genome annotation databases
Ensembl	YEL011W; YEL011W; YEL011W; Saccharomyces cerevisiae. [Genome view]
GeneID	856705.
KEGG	sce:YEL011W.
NMPDR	fig|4932.3.peg.1961.
Organism-specific databases
CYGD	YEL011w.
SGD	S000000737. GLC3.
Phylogenomic databases
eggNOG	fuNOG05596.
HOGENOM	HBG302308.
OMA	YNEYLSR.
OrthoDB	EOG9MCZGQ.
PhylomeDB	P32775.
Enzyme and pathway databases
BRENDA	2.4.1.18. 250.
Gene expression databases
ArrayExpress	P32775.
Genevestigator	P32775.
GermOnline	YEL011W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	982774.

Entry information

Entry name

GLGB_YEAST

Accession

Primary (citable) accession number: P32775

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	February 1, 1995
Last modified:	January 19, 2010
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)