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P32775

- GLGB_YEAST

UniProt

P32775 - GLGB_YEAST

Protein

1,4-alpha-glucan-branching enzyme

Gene

GLC3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 2 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei356 – 3561NucleophileBy similarity
    Active sitei417 – 4171Proton donorBy similarity

    GO - Molecular functioni

    1. 1,4-alpha-glucan branching enzyme activity Source: SGD
    2. cation binding Source: InterPro
    3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

    GO - Biological processi

    1. glycogen biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Glycogen biosynthesis

    Enzyme and pathway databases

    BioCyciYEAST:YEL011W-MONOMER.
    UniPathwayiUPA00164.

    Protein family/group databases

    CAZyiCBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1,4-alpha-glucan-branching enzyme (EC:2.4.1.18)
    Alternative name(s):
    Glycogen-branching enzyme
    Gene namesi
    Name:GLC3
    Ordered Locus Names:YEL011W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYEL011w.
    SGDiS000000737. GLC3.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 7047041,4-alpha-glucan-branching enzymePRO_0000188786Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei190 – 1901Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP32775.
    PaxDbiP32775.
    PeptideAtlasiP32775.

    Expressioni

    Developmental stagei

    Expressed during the transition between the late exponential and stationary growth phases, coincident with maximal glycogen accumulation.

    Gene expression databases

    GenevestigatoriP32775.

    Interactioni

    Protein-protein interaction databases

    BioGridi36720. 11 interactions.
    DIPiDIP-3873N.
    IntActiP32775. 10 interactions.
    MINTiMINT-573503.
    STRINGi4932.YEL011W.

    Structurei

    3D structure databases

    ProteinModelPortaliP32775.
    SMRiP32775. Positions 51-700.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0296.
    GeneTreeiENSGT00390000017040.
    HOGENOMiHOG000175159.
    KOiK00700.
    OMAiNEVIHFL.
    OrthoDBiEOG7647B5.

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000463. GlgB. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P32775-1 [UniParc]FASTAAdd to Basket

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    MYNIPDNVKG AVEFDPWLKP FADVLSERRY LADKWLYDIT HATPDGSYQS    50
    LSKFARDSYK SYGLHANPET KEITYKEWAP NAERAFLVGD FNNWDTTSHE 100
    LKNKDEFGNF TITLHPLPNG DFAIPHDSKI KVMFILPDGS KIFRLPAWIT 150
    RATQPSKETS KQFGPAYEGR FWNPENPYKF VHPRPKFSES VDSLRIYEAH 200
    VGISSPEPKI TTYKEFTEKV LPRIKYLGYD AIQLMAIMEH AYYASFGYQV 250
    TNFFAASSRF GTPEELKELI DTAHSMGILV LLDVVHSHAS KNVEDGLNMF 300
    DGSDHQYFHS ISSGRGEHPL WDSRLFNYGK FEVQRFLLAN LAFYVDVYQF 350
    DGFRFDGVTS MLYVHHGVGA GGSFSGDYNE YLSRDRSFVD HEALAYLMLA 400
    NDLVHEMLPN LAVTVAEDVS GYPTLCLPRS IGGTGFDYRL AMALPDMWIK 450
    LIKEKKDDEW EMGSIVYTLT NRRYGEKVVA YCESHDQALV GDKTLAFWLM 500
    DAAMYTDMTV LKEPSIVIDR GIALHKMIRL ITHSLGGEAY LNFEGNEFGH 550
    PEWLDFPNVN NGDSYKYARR QFNLADDPLL RYQNLNEFDR SMQLCEKRHK 600
    WLNTKQAYVS LKHEGDKMIV FERNNLLFIF NFHPTNSYSD YRVGVEKAGT 650
    YHIVLNSDRA EFGGHNRINE SSEFFTTDLE WNNRKNFLQV YIPSRVALVL 700
    ALKE 704
    Length:704
    Mass (Da):81,116
    Last modified:February 1, 1995 - v2
    Checksum:i9C227E107B825F27
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti564 – 5641S → T in AAA34632. (PubMed:1634552)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M76739 Genomic DNA. Translation: AAA34632.1.
    U18530 Genomic DNA. Translation: AAB64488.1.
    BK006939 Genomic DNA. Translation: DAA07642.1.
    PIRiS50448.
    RefSeqiNP_010905.1. NM_001178826.1.

    Genome annotation databases

    EnsemblFungiiYEL011W; YEL011W; YEL011W.
    GeneIDi856705.
    KEGGisce:YEL011W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M76739 Genomic DNA. Translation: AAA34632.1 .
    U18530 Genomic DNA. Translation: AAB64488.1 .
    BK006939 Genomic DNA. Translation: DAA07642.1 .
    PIRi S50448.
    RefSeqi NP_010905.1. NM_001178826.1.

    3D structure databases

    ProteinModelPortali P32775.
    SMRi P32775. Positions 51-700.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36720. 11 interactions.
    DIPi DIP-3873N.
    IntActi P32775. 10 interactions.
    MINTi MINT-573503.
    STRINGi 4932.YEL011W.

    Protein family/group databases

    CAZyi CBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Proteomic databases

    MaxQBi P32775.
    PaxDbi P32775.
    PeptideAtlasi P32775.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YEL011W ; YEL011W ; YEL011W .
    GeneIDi 856705.
    KEGGi sce:YEL011W.

    Organism-specific databases

    CYGDi YEL011w.
    SGDi S000000737. GLC3.

    Phylogenomic databases

    eggNOGi COG0296.
    GeneTreei ENSGT00390000017040.
    HOGENOMi HOG000175159.
    KOi K00700.
    OMAi NEVIHFL.
    OrthoDBi EOG7647B5.

    Enzyme and pathway databases

    UniPathwayi UPA00164 .
    BioCyci YEAST:YEL011W-MONOMER.

    Miscellaneous databases

    NextBioi 982774.
    PROi P32775.

    Gene expression databases

    Genevestigatori P32775.

    Family and domain databases

    Gene3Di 2.60.40.10. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000463. GlgB. 1 hit.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Coordinate regulation of glycogen metabolism in the yeast Saccharomyces cerevisiae. Induction of glycogen branching enzyme."
      Thon V.J., Vigneron-Lesens C., Marianne-Pepin T., Montreuil J., Decq A., Rachez C., Ball S.G., Cannon J.F.
      J. Biol. Chem. 267:15224-15228(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-190, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGLGB_YEAST
    AccessioniPrimary (citable) accession number: P32775
    Secondary accession number(s): D3DLN8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 128 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1230 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3