Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P32775 (GLGB_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan-branching enzyme

EC=2.4.1.18
Alternative name(s):
Glycogen-branching enzyme
Gene names
Name:GLC3
Ordered Locus Names:YEL011W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length704 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathway

Glycan biosynthesis; glycogen biosynthesis.

Developmental stage

Expressed during the transition between the late exponential and stationary growth phases, coincident with maximal glycogen accumulation.

Miscellaneous

Present with 1230 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7047041,4-alpha-glucan-branching enzyme
PRO_0000188786

Sites

Active site3561Nucleophile By similarity
Active site4171Proton donor By similarity

Amino acid modifications

Modified residue1901Phosphoserine Ref.5

Experimental info

Sequence conflict5641S → T in AAA34632. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P32775 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: 9C227E107B825F27

FASTA70481,116
        10         20         30         40         50         60 
MYNIPDNVKG AVEFDPWLKP FADVLSERRY LADKWLYDIT HATPDGSYQS LSKFARDSYK 

        70         80         90        100        110        120 
SYGLHANPET KEITYKEWAP NAERAFLVGD FNNWDTTSHE LKNKDEFGNF TITLHPLPNG 

       130        140        150        160        170        180 
DFAIPHDSKI KVMFILPDGS KIFRLPAWIT RATQPSKETS KQFGPAYEGR FWNPENPYKF 

       190        200        210        220        230        240 
VHPRPKFSES VDSLRIYEAH VGISSPEPKI TTYKEFTEKV LPRIKYLGYD AIQLMAIMEH 

       250        260        270        280        290        300 
AYYASFGYQV TNFFAASSRF GTPEELKELI DTAHSMGILV LLDVVHSHAS KNVEDGLNMF 

       310        320        330        340        350        360 
DGSDHQYFHS ISSGRGEHPL WDSRLFNYGK FEVQRFLLAN LAFYVDVYQF DGFRFDGVTS 

       370        380        390        400        410        420 
MLYVHHGVGA GGSFSGDYNE YLSRDRSFVD HEALAYLMLA NDLVHEMLPN LAVTVAEDVS 

       430        440        450        460        470        480 
GYPTLCLPRS IGGTGFDYRL AMALPDMWIK LIKEKKDDEW EMGSIVYTLT NRRYGEKVVA 

       490        500        510        520        530        540 
YCESHDQALV GDKTLAFWLM DAAMYTDMTV LKEPSIVIDR GIALHKMIRL ITHSLGGEAY 

       550        560        570        580        590        600 
LNFEGNEFGH PEWLDFPNVN NGDSYKYARR QFNLADDPLL RYQNLNEFDR SMQLCEKRHK 

       610        620        630        640        650        660 
WLNTKQAYVS LKHEGDKMIV FERNNLLFIF NFHPTNSYSD YRVGVEKAGT YHIVLNSDRA 

       670        680        690        700 
EFGGHNRINE SSEFFTTDLE WNNRKNFLQV YIPSRVALVL ALKE 

« Hide

References

« Hide 'large scale' references
[1]"Coordinate regulation of glycogen metabolism in the yeast Saccharomyces cerevisiae. Induction of glycogen branching enzyme."
Thon V.J., Vigneron-Lesens C., Marianne-Pepin T., Montreuil J., Decq A., Rachez C., Ball S.G., Cannon J.F.
J. Biol. Chem. 267:15224-15228(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-190, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M76739 Genomic DNA. Translation: AAA34632.1.
U18530 Genomic DNA. Translation: AAB64488.1.
BK006939 Genomic DNA. Translation: DAA07642.1.
PIRS50448.
RefSeqNP_010905.1. NM_001178826.1.

3D structure databases

ProteinModelPortalP32775.
SMRP32775. Positions 7-704.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36720. 11 interactions.
DIPDIP-3873N.
IntActP32775. 10 interactions.
MINTMINT-573503.
STRING4932.YEL011W.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Proteomic databases

PaxDbP32775.
PeptideAtlasP32775.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYEL011W; YEL011W; YEL011W.
GeneID856705.
KEGGsce:YEL011W.

Organism-specific databases

CYGDYEL011w.
SGDS000000737. GLC3.

Phylogenomic databases

eggNOGCOG0296.
GeneTreeENSGT00390000017040.
HOGENOMHOG000175159.
KOK00700.
OMAEIDPYLK.
OrthoDBEOG7647B5.

Enzyme and pathway databases

BioCycYEAST:YEL011W-MONOMER.
UniPathwayUPA00164.

Gene expression databases

GenevestigatorP32775.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
ProtoNetSearch...

Other

NextBio982774.
PROP32775.

Entry information

Entry nameGLGB_YEAST
AccessionPrimary (citable) accession number: P32775
Secondary accession number(s): D3DLN8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1995
Last modified: March 19, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries