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Protein

Transcription initiation factor IIA subunit 2

Gene

TOA2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-31857-MONOMER.
ReactomeiR-SCE-674695. RNA Polymerase II Pre-transcription Events.
R-SCE-73776. RNA Polymerase II Promoter Escape.
R-SCE-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SCE-75953. RNA Polymerase II Transcription Initiation.
R-SCE-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor IIA subunit 2
Alternative name(s):
General transcription factor IIA subunit 2
TFIIA 13.5 kDa subunit
Transcription initiation factor IIA small chain
Transcription initiation factor IIA small subunit
Gene namesi
Name:TOA2
Ordered Locus Names:YKL058W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL058W.
SGDiS000001541. TOA2.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

  • Note: Imported to nucleus via interaction with KAP122.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • transcription factor TFIIA complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi27I → A or K: Decreases ability to interact with TAF11 and support growth on galactose-containing medium. Unable to support cell viability in a strain deleted for TOA2; when associated with A-69. 1 Publication1
Mutagenesisi41L → D: Decreases ability to interact with Toa1 and TAF11, display mutant growth phenotypes and defects in transcription in vivo. 1 Publication1
Mutagenesisi69Y → A: Unable to support cell viability in a strain deleted for TOA2; when associated with A-27 or K-27. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001940531 – 122Transcription initiation factor IIA subunit 2Add BLAST122

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei95PhosphoserineCombined sources1
Modified residuei102PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32774.
PRIDEiP32774.

PTM databases

iPTMnetiP32774.

Interactioni

Subunit structurei

TFIIA is a heterodimer composed of the large TOA1 and a small TOA2 subunits. Interacts with TBP. Interacts with TAF11. Interacts with KAP122.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SPT15P133932EBI-19323,EBI-19129
TOA1P327733EBI-19323,EBI-19316

Protein-protein interaction databases

BioGridi34075. 32 interactors.
DIPiDIP-2348N.
IntActiP32774. 9 interactors.
MINTiMINT-509274.

Structurei

Secondary structure

1122
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 12Combined sources4
Helixi14 – 28Combined sources15
Helixi34 – 54Combined sources21
Beta strandi59 – 72Combined sources14
Beta strandi75 – 88Combined sources14
Beta strandi104 – 119Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NH2X-ray1.90D2-122[»]
1RM1X-ray2.50B1-122[»]
1YTFX-ray2.50D2-122[»]
5FMFelectron microscopy6.00O1-122[»]
5FYWelectron microscopy4.35V1-122[»]
5FZ5electron microscopy8.80V1-122[»]
5SVAelectron microscopy15.30e1-122[»]
DisProtiDP00009.
ProteinModelPortaliP32774.
SMRiP32774.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32774.

Family & Domainsi

Sequence similaritiesi

Belongs to the TFIIA subunit 2 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000014572.
InParanoidiP32774.
KOiK03123.
OMAiASAYYEF.
OrthoDBiEOG092C5KFB.

Family and domain databases

CDDicd10014. TFIIA_gamma_C. 1 hit.
cd10145. TFIIA_gamma_N. 1 hit.
Gene3Di1.10.287.190. 1 hit.
2.30.18.10. 1 hit.
InterProiIPR009083. TFIIA_a-hlx.
IPR009088. TFIIA_b-brl.
IPR003194. TFIIA_gsu.
IPR015871. TFIIA_gsu_C.
IPR015872. TFIIA_gsu_N.
[Graphical view]
PANTHERiPTHR10966. PTHR10966. 1 hit.
PfamiPF02751. TFIIA_gamma_C. 1 hit.
PF02268. TFIIA_gamma_N. 1 hit.
[Graphical view]
PIRSFiPIRSF009415. Hum_TFIIA_gamma. 1 hit.
ProDomiPD009224. TFIIA_gsu_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF47396. SSF47396. 1 hit.
SSF50784. SSF50784. 1 hit.

Sequencei

Sequence statusi: Complete.

P32774-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVPGYYELY RRSTIGNSLV DALDTLISDG RIEASLAMRV LETFDKVVAE
60 70 80 90 100
TLKDNTQSKL TVKGNLDTYG FCDDVWTFIV KNCQVTVEDS HRDASQNGSG
110 120
DSQSVISVDK LRIVACNSKK SE
Length:122
Mass (Da):13,458
Last modified:October 1, 1993 - v1
Checksum:i5EE90C1BE414F949
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85249 Unassigned DNA. Translation: AAA19653.1.
X75781 Genomic DNA. Translation: CAA53423.1.
Z28058 Genomic DNA. Translation: CAA81895.1.
AY557890 Genomic DNA. Translation: AAS56216.1.
BK006944 Genomic DNA. Translation: DAA09099.1.
PIRiA41810.
RefSeqiNP_012865.1. NM_001179624.1.

Genome annotation databases

EnsemblFungiiYKL058W; YKL058W; YKL058W.
GeneIDi853807.
KEGGisce:YKL058W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85249 Unassigned DNA. Translation: AAA19653.1.
X75781 Genomic DNA. Translation: CAA53423.1.
Z28058 Genomic DNA. Translation: CAA81895.1.
AY557890 Genomic DNA. Translation: AAS56216.1.
BK006944 Genomic DNA. Translation: DAA09099.1.
PIRiA41810.
RefSeqiNP_012865.1. NM_001179624.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NH2X-ray1.90D2-122[»]
1RM1X-ray2.50B1-122[»]
1YTFX-ray2.50D2-122[»]
5FMFelectron microscopy6.00O1-122[»]
5FYWelectron microscopy4.35V1-122[»]
5FZ5electron microscopy8.80V1-122[»]
5SVAelectron microscopy15.30e1-122[»]
DisProtiDP00009.
ProteinModelPortaliP32774.
SMRiP32774.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34075. 32 interactors.
DIPiDIP-2348N.
IntActiP32774. 9 interactors.
MINTiMINT-509274.

PTM databases

iPTMnetiP32774.

Proteomic databases

MaxQBiP32774.
PRIDEiP32774.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL058W; YKL058W; YKL058W.
GeneIDi853807.
KEGGisce:YKL058W.

Organism-specific databases

EuPathDBiFungiDB:YKL058W.
SGDiS000001541. TOA2.

Phylogenomic databases

GeneTreeiENSGT00390000014572.
InParanoidiP32774.
KOiK03123.
OMAiASAYYEF.
OrthoDBiEOG092C5KFB.

Enzyme and pathway databases

BioCyciYEAST:G3O-31857-MONOMER.
ReactomeiR-SCE-674695. RNA Polymerase II Pre-transcription Events.
R-SCE-73776. RNA Polymerase II Promoter Escape.
R-SCE-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SCE-75953. RNA Polymerase II Transcription Initiation.
R-SCE-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Miscellaneous databases

EvolutionaryTraceiP32774.
PROiP32774.

Family and domain databases

CDDicd10014. TFIIA_gamma_C. 1 hit.
cd10145. TFIIA_gamma_N. 1 hit.
Gene3Di1.10.287.190. 1 hit.
2.30.18.10. 1 hit.
InterProiIPR009083. TFIIA_a-hlx.
IPR009088. TFIIA_b-brl.
IPR003194. TFIIA_gsu.
IPR015871. TFIIA_gsu_C.
IPR015872. TFIIA_gsu_N.
[Graphical view]
PANTHERiPTHR10966. PTHR10966. 1 hit.
PfamiPF02751. TFIIA_gamma_C. 1 hit.
PF02268. TFIIA_gamma_N. 1 hit.
[Graphical view]
PIRSFiPIRSF009415. Hum_TFIIA_gamma. 1 hit.
ProDomiPD009224. TFIIA_gsu_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF47396. SSF47396. 1 hit.
SSF50784. SSF50784. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiT2AG_YEAST
AccessioniPrimary (citable) accession number: P32774
Secondary accession number(s): D6VXM9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 30, 2016
This is version 162 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3910 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.