Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transcription initiation factor IIA subunit 2

Gene

TOA2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity.1 Publication

Miscellaneous

Present with 3910 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • RNA polymerase II core promoter sequence-specific DNA binding Source: CAFA
  • TBP-class protein binding Source: CAFA
  • transcription coactivator activity Source: GO_Central

GO - Biological processi

Keywordsi

Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-31857-MONOMER
ReactomeiR-SCE-674695 RNA Polymerase II Pre-transcription Events
R-SCE-6807505 RNA polymerase II transcribes snRNA genes
R-SCE-73776 RNA Polymerase II Promoter Escape
R-SCE-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-SCE-75953 RNA Polymerase II Transcription Initiation
R-SCE-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-SCE-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor IIA subunit 2
Alternative name(s):
General transcription factor IIA subunit 2
TFIIA 13.5 kDa subunit
Transcription initiation factor IIA small chain
Transcription initiation factor IIA small subunit
Gene namesi
Name:TOA2
Ordered Locus Names:YKL058W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL058W
SGDiS000001541 TOA2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi27I → A or K: Decreases ability to interact with TAF11 and support growth on galactose-containing medium. Unable to support cell viability in a strain deleted for TOA2; when associated with A-69. 1 Publication1
Mutagenesisi41L → D: Decreases ability to interact with Toa1 and TAF11, display mutant growth phenotypes and defects in transcription in vivo. 1 Publication1
Mutagenesisi69Y → A: Unable to support cell viability in a strain deleted for TOA2; when associated with A-27 or K-27. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001940531 – 122Transcription initiation factor IIA subunit 2Add BLAST122

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei95PhosphoserineCombined sources1
Modified residuei102PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32774
PaxDbiP32774
PRIDEiP32774

PTM databases

iPTMnetiP32774

Interactioni

Subunit structurei

TFIIA is a heterodimer composed of the large TOA1 and a small TOA2 subunits. Interacts with TBP. Interacts with TAF11. Interacts with KAP122.4 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • TBP-class protein binding Source: CAFA

Protein-protein interaction databases

BioGridi34075, 36 interactors
DIPiDIP-2348N
IntActiP32774, 9 interactors
MINTiP32774
STRINGi4932.YKL058W

Structurei

Secondary structure

1122
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 12Combined sources4
Helixi14 – 28Combined sources15
Helixi34 – 54Combined sources21
Beta strandi59 – 72Combined sources14
Beta strandi75 – 88Combined sources14
Beta strandi104 – 119Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NH2X-ray1.90D2-122[»]
1RM1X-ray2.50B1-122[»]
1YTFX-ray2.50D2-122[»]
5FMFelectron microscopy6.00O1-122[»]
5FYWelectron microscopy4.35V1-122[»]
5FZ5electron microscopy8.80V1-122[»]
5SVAelectron microscopy15.30e1-122[»]
DisProtiDP00009
ProteinModelPortaliP32774
SMRiP32774
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32774

Family & Domainsi

Sequence similaritiesi

Belongs to the TFIIA subunit 2 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000014572
InParanoidiP32774
KOiK03123
OMAiFKMESNE
OrthoDBiEOG092C5KFB

Family and domain databases

CDDicd10014 TFIIA_gamma_C, 1 hit
cd10145 TFIIA_gamma_N, 1 hit
Gene3Di1.10.287.190, 1 hit
2.30.18.10, 1 hit
InterProiView protein in InterPro
IPR009083 TFIIA_a-hlx
IPR009088 TFIIA_b-brl
IPR003194 TFIIA_gsu
IPR015871 TFIIA_gsu_C
IPR015872 TFIIA_gsu_N
PANTHERiPTHR10966 PTHR10966, 1 hit
PfamiView protein in Pfam
PF02751 TFIIA_gamma_C, 1 hit
PF02268 TFIIA_gamma_N, 1 hit
PIRSFiPIRSF009415 Hum_TFIIA_gamma, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD009224 TFIIA_gsu_N, 1 hit
SUPFAMiSSF50784 SSF50784, 1 hit

Sequencei

Sequence statusi: Complete.

P32774-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVPGYYELY RRSTIGNSLV DALDTLISDG RIEASLAMRV LETFDKVVAE
60 70 80 90 100
TLKDNTQSKL TVKGNLDTYG FCDDVWTFIV KNCQVTVEDS HRDASQNGSG
110 120
DSQSVISVDK LRIVACNSKK SE
Length:122
Mass (Da):13,458
Last modified:October 1, 1993 - v1
Checksum:i5EE90C1BE414F949
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85249 Unassigned DNA Translation: AAA19653.1
X75781 Genomic DNA Translation: CAA53423.1
Z28058 Genomic DNA Translation: CAA81895.1
AY557890 Genomic DNA Translation: AAS56216.1
BK006944 Genomic DNA Translation: DAA09099.1
PIRiA41810
RefSeqiNP_012865.1, NM_001179624.1

Genome annotation databases

EnsemblFungiiYKL058W; YKL058W; YKL058W
GeneIDi853807
KEGGisce:YKL058W

Similar proteinsi

Entry informationi

Entry nameiT2AG_YEAST
AccessioniPrimary (citable) accession number: P32774
Secondary accession number(s): D6VXM9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 23, 2018
This is version 173 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health