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P32774 (T2AG_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 139. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription initiation factor IIA subunit 2
Alternative name(s):
General transcription factor IIA subunit 2
TFIIA 13.5 kDa subunit
Transcription initiation factor IIA small chain
Transcription initiation factor IIA small subunit
Gene names
Name:TOA2
Ordered Locus Names:YKL058W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length122 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity. Ref.6

Subunit structure

TFIIA is a heterodimer composed of the large TOA1 and a small TOA2 subunits. Interacts with TBP. Interacts with TAF11. Interacts with KAP122. Ref.6 Ref.7 Ref.8

Subcellular location

Cytoplasm. Nucleus. Note: Imported to nucleus via interaction with KAP122. Ref.7

Miscellaneous

Present with 3910 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the TFIIA subunit 2 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SPT15P133932EBI-19323,EBI-19129
TOA1P327733EBI-19323,EBI-19316

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 122122Transcription initiation factor IIA subunit 2
PRO_0000194053

Amino acid modifications

Modified residue951Phosphoserine Ref.10 Ref.11
Modified residue1021Phosphoserine Ref.10

Experimental info

Mutagenesis271I → A or K: Decreases ability to interact with TAF11 and support growth on galactose-containing medium. Unable to support cell viability in a strain deleted for TOA2; when associated with A-69. Ref.8
Mutagenesis411L → D: Decreases ability to interact with Toa1 and TAF11, display mutant growth phenotypes and defects in transcription in vivo. Ref.8
Mutagenesis691Y → A: Unable to support cell viability in a strain deleted for TOA2; when associated with A-27 or K-27.

Secondary structure

............. 122
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P32774 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 5EE90C1BE414F949

FASTA12213,458
        10         20         30         40         50         60 
MAVPGYYELY RRSTIGNSLV DALDTLISDG RIEASLAMRV LETFDKVVAE TLKDNTQSKL 

        70         80         90        100        110        120 
TVKGNLDTYG FCDDVWTFIV KNCQVTVEDS HRDASQNGSG DSQSVISVDK LRIVACNSKK 


SE 

« Hide

References

« Hide 'large scale' references
[1]"Isolation of two genes that encode subunits of the yeast transcription factor IIA."
Ranish J.A., Lane W.S., Hahn S.
Science 255:1127-1129(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 15-30 AND 40-58.
[2]"Sequence of a 28.6 kb region of yeast chromosome XI includes the FBA1 and TOA2 genes, an open reading frame (ORF) similar to a translationally controlled tumour protein, one ORF containing motifs also found in plant storage proteins and 13 ORFs with weak or no homology to known proteins."
Rasmussen S.W.
Yeast 10:S63-S68(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"The yeast general transcription factor TFIIA is composed of two polypeptide subunits."
Ranish J.A., Hahn S.
J. Biol. Chem. 266:19320-19327(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT.
[7]"The karyopherin Kap122p/Pdr6p imports both subunits of the transcription factor IIA into the nucleus."
Titov A.A., Blobel G.
J. Cell Biol. 147:235-246(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH KAP122 AND TOA1, SUBCELLULAR LOCATION.
[8]"TFIIA interacts with TFIID via association with TATA-binding protein and TAF40."
Kraemer S.M., Ranallo R.T., Ogg R.C., Stargell L.A.
Mol. Cell. Biol. 21:1737-1746(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF ILE-27 AND LEU-41, INTERACTION WITH TAF11.
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-95 AND SER-102, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-95, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Crystal structure of a yeast TFIIA/TBP/DNA complex."
Tan S., Hunziker Y., Sargent D.F., Richmond T.J.
Nature 381:127-134(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) IN COMPLEX WITH TOA1; TBP AND DNA.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M85249 Unassigned DNA. Translation: AAA19653.1.
X75781 Genomic DNA. Translation: CAA53423.1.
Z28058 Genomic DNA. Translation: CAA81895.1.
AY557890 Genomic DNA. Translation: AAS56216.1.
BK006944 Genomic DNA. Translation: DAA09099.1.
PIRA41810.
RefSeqNP_012865.1. NM_001179624.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1NH2X-ray1.90D2-122[»]
1RM1X-ray2.50B1-122[»]
1YTFX-ray2.50D2-122[»]
DisProtDP00009.
ProteinModelPortalP32774.
SMRP32774. Positions 5-121.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34075. 33 interactions.
DIPDIP-2348N.
IntActP32774. 9 interactions.
MINTMINT-509274.
STRING4932.YKL058W.

Proteomic databases

PaxDbP32774.
PeptideAtlasP32774.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL058W; YKL058W; YKL058W.
GeneID853807.
KEGGsce:YKL058W.

Organism-specific databases

CYGDYKL058w.
SGDS000001541. TOA2.

Phylogenomic databases

eggNOGCOG5123.
GeneTreeENSGT00390000014572.
KOK03123.
OMAWTFIVKN.
OrthoDBEOG7K6Q74.

Enzyme and pathway databases

BioCycYEAST:G3O-31857-MONOMER.

Gene expression databases

GenevestigatorP32774.

Family and domain databases

Gene3D1.10.287.190. 1 hit.
2.30.18.10. 1 hit.
InterProIPR009083. TFIIA_a-hlx.
IPR009088. TFIIA_b-brl.
IPR003194. TFIIA_gsu.
IPR015871. TFIIA_gsu_C.
IPR015872. TFIIA_gsu_N.
[Graphical view]
PANTHERPTHR10966. PTHR10966. 1 hit.
PfamPF02751. TFIIA_gamma_C. 1 hit.
PF02268. TFIIA_gamma_N. 1 hit.
[Graphical view]
PIRSFPIRSF009415. Hum_TFIIA_gamma. 1 hit.
ProDomPD009224. TFIIA_gsu_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF47396. SSF47396. 1 hit.
SSF50784. SSF50784. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP32774.
NextBio974966.
PROP32774.

Entry information

Entry nameT2AG_YEAST
AccessionPrimary (citable) accession number: P32774
Secondary accession number(s): D6VXM9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: April 16, 2014
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references