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Protein

Transcription initiation factor IIA subunit 2

Gene

TOA2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-31857-MONOMER.
ReactomeiR-SCE-674695. RNA Polymerase II Pre-transcription Events.
R-SCE-73776. RNA Polymerase II Promoter Escape.
R-SCE-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SCE-75953. RNA Polymerase II Transcription Initiation.
R-SCE-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor IIA subunit 2
Alternative name(s):
General transcription factor IIA subunit 2
TFIIA 13.5 kDa subunit
Transcription initiation factor IIA small chain
Transcription initiation factor IIA small subunit
Gene namesi
Name:TOA2
Ordered Locus Names:YKL058W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL058W.
SGDiS000001541. TOA2.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

  • Note: Imported to nucleus via interaction with KAP122.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • transcription factor TFIIA complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi27 – 271I → A or K: Decreases ability to interact with TAF11 and support growth on galactose-containing medium. Unable to support cell viability in a strain deleted for TOA2; when associated with A-69. 1 Publication
Mutagenesisi41 – 411L → D: Decreases ability to interact with Toa1 and TAF11, display mutant growth phenotypes and defects in transcription in vivo. 1 Publication
Mutagenesisi69 – 691Y → A: Unable to support cell viability in a strain deleted for TOA2; when associated with A-27 or K-27.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 122122Transcription initiation factor IIA subunit 2PRO_0000194053Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei95 – 951PhosphoserineCombined sources
Modified residuei102 – 1021PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32774.

PTM databases

iPTMnetiP32774.

Interactioni

Subunit structurei

TFIIA is a heterodimer composed of the large TOA1 and a small TOA2 subunits. Interacts with TBP. Interacts with TAF11. Interacts with KAP122.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SPT15P133932EBI-19323,EBI-19129
TOA1P327733EBI-19323,EBI-19316

Protein-protein interaction databases

BioGridi34075. 32 interactions.
DIPiDIP-2348N.
IntActiP32774. 9 interactions.
MINTiMINT-509274.

Structurei

Secondary structure

1
122
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 124Combined sources
Helixi14 – 2815Combined sources
Helixi34 – 5421Combined sources
Beta strandi59 – 7214Combined sources
Beta strandi75 – 8814Combined sources
Beta strandi104 – 11916Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NH2X-ray1.90D2-122[»]
1RM1X-ray2.50B1-122[»]
1YTFX-ray2.50D2-122[»]
5FMFelectron microscopy6.00O1-122[»]
5FYWelectron microscopy4.35V1-122[»]
5FZ5electron microscopy8.80V1-122[»]
DisProtiDP00009.
ProteinModelPortaliP32774.
SMRiP32774. Positions 5-121.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32774.

Family & Domainsi

Sequence similaritiesi

Belongs to the TFIIA subunit 2 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000014572.
InParanoidiP32774.
KOiK03123.
OMAiKEHGTSN.
OrthoDBiEOG7K6Q74.

Family and domain databases

Gene3Di1.10.287.190. 1 hit.
2.30.18.10. 1 hit.
InterProiIPR009083. TFIIA_a-hlx.
IPR009088. TFIIA_b-brl.
IPR003194. TFIIA_gsu.
IPR015871. TFIIA_gsu_C.
IPR015872. TFIIA_gsu_N.
[Graphical view]
PANTHERiPTHR10966. PTHR10966. 1 hit.
PfamiPF02751. TFIIA_gamma_C. 1 hit.
PF02268. TFIIA_gamma_N. 1 hit.
[Graphical view]
PIRSFiPIRSF009415. Hum_TFIIA_gamma. 1 hit.
ProDomiPD009224. TFIIA_gsu_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF47396. SSF47396. 1 hit.
SSF50784. SSF50784. 1 hit.

Sequencei

Sequence statusi: Complete.

P32774-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVPGYYELY RRSTIGNSLV DALDTLISDG RIEASLAMRV LETFDKVVAE
60 70 80 90 100
TLKDNTQSKL TVKGNLDTYG FCDDVWTFIV KNCQVTVEDS HRDASQNGSG
110 120
DSQSVISVDK LRIVACNSKK SE
Length:122
Mass (Da):13,458
Last modified:October 1, 1993 - v1
Checksum:i5EE90C1BE414F949
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85249 Unassigned DNA. Translation: AAA19653.1.
X75781 Genomic DNA. Translation: CAA53423.1.
Z28058 Genomic DNA. Translation: CAA81895.1.
AY557890 Genomic DNA. Translation: AAS56216.1.
BK006944 Genomic DNA. Translation: DAA09099.1.
PIRiA41810.
RefSeqiNP_012865.1. NM_001179624.1.

Genome annotation databases

EnsemblFungiiYKL058W; YKL058W; YKL058W.
GeneIDi853807.
KEGGisce:YKL058W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85249 Unassigned DNA. Translation: AAA19653.1.
X75781 Genomic DNA. Translation: CAA53423.1.
Z28058 Genomic DNA. Translation: CAA81895.1.
AY557890 Genomic DNA. Translation: AAS56216.1.
BK006944 Genomic DNA. Translation: DAA09099.1.
PIRiA41810.
RefSeqiNP_012865.1. NM_001179624.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NH2X-ray1.90D2-122[»]
1RM1X-ray2.50B1-122[»]
1YTFX-ray2.50D2-122[»]
5FMFelectron microscopy6.00O1-122[»]
5FYWelectron microscopy4.35V1-122[»]
5FZ5electron microscopy8.80V1-122[»]
DisProtiDP00009.
ProteinModelPortaliP32774.
SMRiP32774. Positions 5-121.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34075. 32 interactions.
DIPiDIP-2348N.
IntActiP32774. 9 interactions.
MINTiMINT-509274.

PTM databases

iPTMnetiP32774.

Proteomic databases

MaxQBiP32774.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL058W; YKL058W; YKL058W.
GeneIDi853807.
KEGGisce:YKL058W.

Organism-specific databases

EuPathDBiFungiDB:YKL058W.
SGDiS000001541. TOA2.

Phylogenomic databases

GeneTreeiENSGT00390000014572.
InParanoidiP32774.
KOiK03123.
OMAiKEHGTSN.
OrthoDBiEOG7K6Q74.

Enzyme and pathway databases

BioCyciYEAST:G3O-31857-MONOMER.
ReactomeiR-SCE-674695. RNA Polymerase II Pre-transcription Events.
R-SCE-73776. RNA Polymerase II Promoter Escape.
R-SCE-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SCE-75953. RNA Polymerase II Transcription Initiation.
R-SCE-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Miscellaneous databases

EvolutionaryTraceiP32774.
PROiP32774.

Family and domain databases

Gene3Di1.10.287.190. 1 hit.
2.30.18.10. 1 hit.
InterProiIPR009083. TFIIA_a-hlx.
IPR009088. TFIIA_b-brl.
IPR003194. TFIIA_gsu.
IPR015871. TFIIA_gsu_C.
IPR015872. TFIIA_gsu_N.
[Graphical view]
PANTHERiPTHR10966. PTHR10966. 1 hit.
PfamiPF02751. TFIIA_gamma_C. 1 hit.
PF02268. TFIIA_gamma_N. 1 hit.
[Graphical view]
PIRSFiPIRSF009415. Hum_TFIIA_gamma. 1 hit.
ProDomiPD009224. TFIIA_gsu_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF47396. SSF47396. 1 hit.
SSF50784. SSF50784. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of two genes that encode subunits of the yeast transcription factor IIA."
    Ranish J.A., Lane W.S., Hahn S.
    Science 255:1127-1129(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 15-30 AND 40-58.
  2. "Sequence of a 28.6 kb region of yeast chromosome XI includes the FBA1 and TOA2 genes, an open reading frame (ORF) similar to a translationally controlled tumour protein, one ORF containing motifs also found in plant storage proteins and 13 ORFs with weak or no homology to known proteins."
    Rasmussen S.W.
    Yeast 10:S63-S68(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "The yeast general transcription factor TFIIA is composed of two polypeptide subunits."
    Ranish J.A., Hahn S.
    J. Biol. Chem. 266:19320-19327(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  7. "The karyopherin Kap122p/Pdr6p imports both subunits of the transcription factor IIA into the nucleus."
    Titov A.A., Blobel G.
    J. Cell Biol. 147:235-246(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KAP122 AND TOA1, SUBCELLULAR LOCATION.
  8. "TFIIA interacts with TFIID via association with TATA-binding protein and TAF40."
    Kraemer S.M., Ranallo R.T., Ogg R.C., Stargell L.A.
    Mol. Cell. Biol. 21:1737-1746(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ILE-27 AND LEU-41, INTERACTION WITH TAF11.
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-95 AND SER-102, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-95, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Crystal structure of a yeast TFIIA/TBP/DNA complex."
    Tan S., Hunziker Y., Sargent D.F., Richmond T.J.
    Nature 381:127-134(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) IN COMPLEX WITH TOA1; TBP AND DNA.

Entry informationi

Entry nameiT2AG_YEAST
AccessioniPrimary (citable) accession number: P32774
Secondary accession number(s): D6VXM9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: July 6, 2016
This is version 158 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3910 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.