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Protein

Transcription initiation factor IIA large subunit

Gene

TOA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

TFIIA is a component of the transcription machinery of RNA polymerase II and implicated in the regulation of basal transcription. Interacts with TBP (the TATA-binding protein).

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription

Enzyme and pathway databases

BioCyciYEAST:G3O-33703-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor IIA large subunit
Short name:
TFIIA large subunit
Alternative name(s):
TFIIA 32 kDa subunit
Gene namesi
Name:TOA1
Ordered Locus Names:YOR194C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR194C.
SGDiS000005720. TOA1.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

  • Note: Imported to nucleus via interaction with KAP122.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • transcription factor TFIIA complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 286286Transcription initiation factor IIA large subunitPRO_0000072617Add
BLAST

Proteomic databases

MaxQBiP32773.
TopDownProteomicsiP32773.

PTM databases

iPTMnetiP32773.

Interactioni

Subunit structurei

TFIIA is a heterodimer composed of the large TOA1 and a small TOA2 subunits. Interacts with KAP122.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
TOA2P327743EBI-19316,EBI-19323

Protein-protein interaction databases

BioGridi34592. 21 interactions.
DIPiDIP-2347N.
IntActiP32773. 2 interactions.
MINTiMINT-503105.

Structurei

Secondary structure

1
286
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 2017Combined sources
Helixi22 – 276Combined sources
Helixi32 – 4716Combined sources
Helixi57 – 604Combined sources
Beta strandi243 – 25412Combined sources
Beta strandi257 – 26913Combined sources
Beta strandi272 – 28413Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NH2X-ray1.90B2-54[»]
C210-286[»]
1RM1X-ray2.50C1-286[»]
1YTFX-ray2.50B2-54[»]
C210-286[»]
5FMFelectron microscopy6.00M2-286[»]
5FYWelectron microscopy4.35U1-286[»]
5FZ5electron microscopy8.80U1-286[»]
DisProtiDP00104.
ProteinModelPortaliP32773.
SMRiP32773. Positions 2-47, 182-286.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32773.

Family & Domainsi

Sequence similaritiesi

Belongs to the TFIIA subunit 1 family.Curated

Phylogenomic databases

HOGENOMiHOG000246665.
InParanoidiP32773.
KOiK03122.
OMAiDSRQDFE.
OrthoDBiEOG092C56EC.

Family and domain databases

CDDicd07976. TFIIA_alpha_beta_like. 1 hit.
Gene3Di1.10.287.100. 1 hit.
2.30.18.10. 1 hit.
InterProiIPR009083. TFIIA_a-hlx.
IPR004855. TFIIA_asu/bsu.
IPR013028. TFIIA_asu_N.
IPR009088. TFIIA_b-brl.
[Graphical view]
PANTHERiPTHR12694. PTHR12694. 1 hit.
PfamiPF03153. TFIIA. 2 hits.
[Graphical view]
SMARTiSM01371. TFIIA. 1 hit.
[Graphical view]
SUPFAMiSSF47396. SSF47396. 1 hit.
SSF50784. SSF50784. 1 hit.

Sequencei

Sequence statusi: Complete.

P32773-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNAEASRVY EIIVESVVNE VREDFENAGI DEQTLQDLKN IWQKKLTETK
60 70 80 90 100
VTTFSWDNQF NEGNINGVQN DLNFNLATPG VNSSEFNIKE ENTGNEGLIL
110 120 130 140 150
PNINSNNNIP HSGETNINTN TVEATNNSGA TLNTNTSGNT NADVTSQPKI
160 170 180 190 200
EVKPEIELTI NNANITTVEN IDDESEKKDD EEKEEDVEKT RKEKEQIEQV
210 220 230 240 250
KLQAKKEKRS ALLDTDEVGS ELDDSDDDYL ISEGEEDGPD ENLMLCLYDK
260 270 280
VTRTKARWKC SLKDGVVTIN RNDYTFQKAQ VEAEWV
Length:286
Mass (Da):32,201
Last modified:October 1, 1993 - v1
Checksum:i5BF0C39581866C98
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti15 – 151E → Q in AAT93242 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85248 Unassigned DNA. Translation: AAA19654.1.
Z75102 Genomic DNA. Translation: CAA99407.1.
AY693223 Genomic DNA. Translation: AAT93242.1.
BK006948 Genomic DNA. Translation: DAA10969.1.
PIRiB41810.
RefSeqiNP_014837.1. NM_001183613.1.

Genome annotation databases

EnsemblFungiiYOR194C; YOR194C; YOR194C.
GeneIDi854369.
KEGGisce:YOR194C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85248 Unassigned DNA. Translation: AAA19654.1.
Z75102 Genomic DNA. Translation: CAA99407.1.
AY693223 Genomic DNA. Translation: AAT93242.1.
BK006948 Genomic DNA. Translation: DAA10969.1.
PIRiB41810.
RefSeqiNP_014837.1. NM_001183613.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NH2X-ray1.90B2-54[»]
C210-286[»]
1RM1X-ray2.50C1-286[»]
1YTFX-ray2.50B2-54[»]
C210-286[»]
5FMFelectron microscopy6.00M2-286[»]
5FYWelectron microscopy4.35U1-286[»]
5FZ5electron microscopy8.80U1-286[»]
DisProtiDP00104.
ProteinModelPortaliP32773.
SMRiP32773. Positions 2-47, 182-286.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34592. 21 interactions.
DIPiDIP-2347N.
IntActiP32773. 2 interactions.
MINTiMINT-503105.

PTM databases

iPTMnetiP32773.

Proteomic databases

MaxQBiP32773.
TopDownProteomicsiP32773.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR194C; YOR194C; YOR194C.
GeneIDi854369.
KEGGisce:YOR194C.

Organism-specific databases

EuPathDBiFungiDB:YOR194C.
SGDiS000005720. TOA1.

Phylogenomic databases

HOGENOMiHOG000246665.
InParanoidiP32773.
KOiK03122.
OMAiDSRQDFE.
OrthoDBiEOG092C56EC.

Enzyme and pathway databases

BioCyciYEAST:G3O-33703-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP32773.
PROiP32773.

Family and domain databases

CDDicd07976. TFIIA_alpha_beta_like. 1 hit.
Gene3Di1.10.287.100. 1 hit.
2.30.18.10. 1 hit.
InterProiIPR009083. TFIIA_a-hlx.
IPR004855. TFIIA_asu/bsu.
IPR013028. TFIIA_asu_N.
IPR009088. TFIIA_b-brl.
[Graphical view]
PANTHERiPTHR12694. PTHR12694. 1 hit.
PfamiPF03153. TFIIA. 2 hits.
[Graphical view]
SMARTiSM01371. TFIIA. 1 hit.
[Graphical view]
SUPFAMiSSF47396. SSF47396. 1 hit.
SSF50784. SSF50784. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTOA1_YEAST
AccessioniPrimary (citable) accession number: P32773
Secondary accession number(s): D6W2Q3, E9P936
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: September 7, 2016
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.