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Protein

Transcription initiation factor IIA large subunit

Gene

TOA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

TFIIA is a component of the transcription machinery of RNA polymerase II and implicated in the regulation of basal transcription. Interacts with TBP (the TATA-binding protein).

GO - Molecular functioni

  • RNA polymerase II core promoter sequence-specific DNA binding Source: CAFA
  • TBP-class protein binding Source: CAFA

GO - Biological processi

Keywordsi

Biological processTranscription

Enzyme and pathway databases

BioCyciYEAST:G3O-33703-MONOMER
ReactomeiR-SCE-674695 RNA Polymerase II Pre-transcription Events
R-SCE-6807505 RNA polymerase II transcribes snRNA genes
R-SCE-73776 RNA Polymerase II Promoter Escape
R-SCE-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-SCE-75953 RNA Polymerase II Transcription Initiation
R-SCE-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-SCE-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor IIA large subunit
Short name:
TFIIA large subunit
Alternative name(s):
TFIIA 32 kDa subunit
Gene namesi
Name:TOA1
Ordered Locus Names:YOR194C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi

Organism-specific databases

EuPathDBiFungiDB:YOR194C
SGDiS000005720 TOA1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000726171 – 286Transcription initiation factor IIA large subunitAdd BLAST286

Proteomic databases

MaxQBiP32773
PaxDbiP32773
PRIDEiP32773
TopDownProteomicsiP32773

PTM databases

iPTMnetiP32773

Interactioni

Subunit structurei

TFIIA is a heterodimer composed of the large TOA1 and a small TOA2 subunits. Interacts with KAP122.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
TOA2P327742EBI-19316,EBI-19323

GO - Molecular functioni

  • TBP-class protein binding Source: CAFA

Protein-protein interaction databases

BioGridi34592, 269 interactors
DIPiDIP-2347N
IntActiP32773, 2 interactors
MINTiP32773
STRINGi4932.YOR194C

Structurei

Secondary structure

1286
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 20Combined sources17
Helixi22 – 27Combined sources6
Helixi32 – 47Combined sources16
Helixi57 – 60Combined sources4
Beta strandi243 – 254Combined sources12
Beta strandi257 – 269Combined sources13
Beta strandi272 – 284Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NH2X-ray1.90B2-54[»]
C210-286[»]
1RM1X-ray2.50C1-286[»]
1YTFX-ray2.50B2-54[»]
C210-286[»]
5FMFelectron microscopy6.00M2-286[»]
5FYWelectron microscopy4.35U1-286[»]
5FZ5electron microscopy8.80U1-286[»]
5SVAelectron microscopy15.30d1-286[»]
DisProtiDP00104
ProteinModelPortaliP32773
SMRiP32773
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32773

Family & Domainsi

Sequence similaritiesi

Belongs to the TFIIA subunit 1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00900000141061
HOGENOMiHOG000246665
InParanoidiP32773
KOiK03122
OMAiVAKFSWD
OrthoDBiEOG092C56EC

Family and domain databases

CDDicd07976 TFIIA_alpha_beta_like, 1 hit
Gene3Di2.30.18.10, 1 hit
InterProiView protein in InterPro
IPR004855 TFIIA_asu/bsu
IPR009088 TFIIA_b-brl
PfamiView protein in Pfam
PF03153 TFIIA, 2 hits
SMARTiView protein in SMART
SM01371 TFIIA, 1 hit
SUPFAMiSSF50784 SSF50784, 1 hit

Sequencei

Sequence statusi: Complete.

P32773-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNAEASRVY EIIVESVVNE VREDFENAGI DEQTLQDLKN IWQKKLTETK
60 70 80 90 100
VTTFSWDNQF NEGNINGVQN DLNFNLATPG VNSSEFNIKE ENTGNEGLIL
110 120 130 140 150
PNINSNNNIP HSGETNINTN TVEATNNSGA TLNTNTSGNT NADVTSQPKI
160 170 180 190 200
EVKPEIELTI NNANITTVEN IDDESEKKDD EEKEEDVEKT RKEKEQIEQV
210 220 230 240 250
KLQAKKEKRS ALLDTDEVGS ELDDSDDDYL ISEGEEDGPD ENLMLCLYDK
260 270 280
VTRTKARWKC SLKDGVVTIN RNDYTFQKAQ VEAEWV
Length:286
Mass (Da):32,201
Last modified:October 1, 1993 - v1
Checksum:i5BF0C39581866C98
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15E → Q in AAT93242 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85248 Unassigned DNA Translation: AAA19654.1
Z75102 Genomic DNA Translation: CAA99407.1
AY693223 Genomic DNA Translation: AAT93242.1
BK006948 Genomic DNA Translation: DAA10969.1
PIRiB41810
RefSeqiNP_014837.1, NM_001183613.1

Genome annotation databases

EnsemblFungiiYOR194C; YOR194C; YOR194C
GeneIDi854369
KEGGisce:YOR194C

Similar proteinsi

Entry informationi

Entry nameiTOA1_YEAST
AccessioniPrimary (citable) accession number: P32773
Secondary accession number(s): D6W2Q3, E9P936
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 23, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

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