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P32771

- FADH_YEAST

UniProt

P32771 - FADH_YEAST

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Protein
S-(hydroxymethyl)glutathione dehydrogenase
Gene
SFA1, SFA, YDL168W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Oxidizes long-chain alcohols and, in the presence of glutathione, is able to oxidize formaldehyde. Is responsible for yeast resistance to formaldehyde.

Catalytic activityi

S-(hydroxymethyl)glutathione + NAD(P)+ = S-formylglutathione + NAD(P)H.
An alcohol + NAD+ = an aldehyde or ketone + NADH.

Cofactori

Binds 2 zinc ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi49 – 491Zinc 1; catalytic By similarity
Metal bindingi71 – 711Zinc 1; catalytic By similarity
Metal bindingi101 – 1011Zinc 2 By similarity
Metal bindingi104 – 1041Zinc 2 By similarity
Metal bindingi107 – 1071Zinc 2 By similarity
Metal bindingi115 – 1151Zinc 2 By similarity
Metal bindingi179 – 1791Zinc 1; catalytic By similarity

GO - Molecular functioni

  1. S-(hydroxymethyl)glutathione dehydrogenase activity Source: SGD
  2. alcohol dehydrogenase (NAD) activity Source: SGD
  3. hydroxymethylfurfural reductase (NADH) activity Source: SGD
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. amino acid catabolic process to alcohol via Ehrlich pathway Source: SGD
  2. ethanol oxidation Source: InterPro
  3. formaldehyde catabolic process Source: SGD
  4. furaldehyde metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-11840.
YEAST:YDL168W-MONOMER.
ReactomeiREACT_188930. Abacavir metabolism.
REACT_86838. Ethanol oxidation.

Names & Taxonomyi

Protein namesi
Recommended name:
S-(hydroxymethyl)glutathione dehydrogenase (EC:1.1.1.284)
Alternative name(s):
Alcohol dehydrogenase SFA (EC:1.1.1.1)
Glutathione-dependent formaldehyde dehydrogenase (EC:1.1.1.-)
Short name:
FALDH
Short name:
FDH
Short name:
FLD
Short name:
GSH-FDH
Gene namesi
Name:SFA1
Synonyms:SFA
Ordered Locus Names:YDL168W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDL168w.
SGDiS000002327. SFA1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 386386S-(hydroxymethyl)glutathione dehydrogenase
PRO_0000160785Add
BLAST

Proteomic databases

MaxQBiP32771.
PaxDbiP32771.
PeptideAtlasiP32771.

Expressioni

Inductioni

By formaldehyde, ethanol and methyl methanesulphonate.

Gene expression databases

GenevestigatoriP32771.

Interactioni

Protein-protein interaction databases

BioGridi31897. 77 interactions.
DIPiDIP-5366N.
IntActiP32771. 2 interactions.
MINTiMINT-539686.
STRINGi4932.YDL168W.

Structurei

3D structure databases

ProteinModelPortaliP32771.
SMRiP32771. Positions 10-381.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1062.
GeneTreeiENSGT00430000030800.
HOGENOMiHOG000294674.
KOiK00121.
OMAiAWKSGAP.
OrthoDBiEOG75F4PS.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR014183. ADH_3.
IPR013149. ADH_C.
IPR013154. ADH_GroES-like.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 2 hits.
TIGRFAMsiTIGR02818. adh_III_F_hyde. 1 hit.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32771-1 [UniParc]FASTAAdd to Basket

« Hide

MSAATVGKPI KCIAAVAYDA KKPLSVEEIT VDAPKAHEVR IKIEYTAVCH    50
TDAYTLSGSD PEGLFPCVLG HEGAGIVESV GDDVITVKPG DHVIALYTAE 100
CGKCKFCTSG KTNLCGAVRA TQGKGVMPDG TTRFHNAKGE DIYHFMGCST 150
FSEYTVVADV SVVAIDPKAP LDAACLLGCG VTTGFGAALK TANVQKGDTV 200
AVFGCGTVGL SVIQGAKLRG ASKIIAIDIN NKKKQYCSQF GATDFVNPKE 250
DLAKDQTIVE KLIEMTDGGL DFTFDCTGNT KIMRDALEAC HKGWGQSIII 300
GVAAAGEEIS TRPFQLVTGR VWKGSAFGGI KGRSEMGGLI KDYQKGALKV 350
EEFITHRRPF KEINQAFEDL HNGDCLRTVL KSDEIK 386
Length:386
Mass (Da):41,042
Last modified:October 1, 1993 - v1
Checksum:iA495AFD7B47E4B11
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X68020 Genomic DNA. Translation: CAA48161.1.
Z67750 Genomic DNA. Translation: CAA91578.1.
Z74216 Genomic DNA. Translation: CAA98742.1.
BK006938 Genomic DNA. Translation: DAA11693.1.
PIRiS31140.
RefSeqiNP_010113.1. NM_001180228.1.

Genome annotation databases

EnsemblFungiiYDL168W; YDL168W; YDL168W.
GeneIDi851386.
KEGGisce:YDL168W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X68020 Genomic DNA. Translation: CAA48161.1 .
Z67750 Genomic DNA. Translation: CAA91578.1 .
Z74216 Genomic DNA. Translation: CAA98742.1 .
BK006938 Genomic DNA. Translation: DAA11693.1 .
PIRi S31140.
RefSeqi NP_010113.1. NM_001180228.1.

3D structure databases

ProteinModelPortali P32771.
SMRi P32771. Positions 10-381.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31897. 77 interactions.
DIPi DIP-5366N.
IntActi P32771. 2 interactions.
MINTi MINT-539686.
STRINGi 4932.YDL168W.

Proteomic databases

MaxQBi P32771.
PaxDbi P32771.
PeptideAtlasi P32771.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDL168W ; YDL168W ; YDL168W .
GeneIDi 851386.
KEGGi sce:YDL168W.

Organism-specific databases

CYGDi YDL168w.
SGDi S000002327. SFA1.

Phylogenomic databases

eggNOGi COG1062.
GeneTreei ENSGT00430000030800.
HOGENOMi HOG000294674.
KOi K00121.
OMAi AWKSGAP.
OrthoDBi EOG75F4PS.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-11840.
YEAST:YDL168W-MONOMER.
Reactomei REACT_188930. Abacavir metabolism.
REACT_86838. Ethanol oxidation.

Miscellaneous databases

NextBioi 968534.
PROi P32771.

Gene expression databases

Genevestigatori P32771.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProi IPR014183. ADH_3.
IPR013149. ADH_C.
IPR013154. ADH_GroES-like.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR11695. PTHR11695. 1 hit.
Pfami PF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view ]
SUPFAMi SSF50129. SSF50129. 2 hits.
TIGRFAMsi TIGR02818. adh_III_F_hyde. 1 hit.
PROSITEi PS00059. ADH_ZINC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular structure and genetic regulation of SFA, a gene responsible for resistance to formaldehyde in Saccharomyces cerevisiae, and characterization of its protein product."
    Wehner E.P., Rao E., Brendel M.
    Mol. Gen. Genet. 237:351-358(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 38626 / AH22 / NRRL Y-12843.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiFADH_YEAST
AccessioniPrimary (citable) accession number: P32771
Secondary accession number(s): D6VRI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: September 3, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Requires GSH for oxidation of formaldehyde or of methylglyoxal, while oxidation of long-chain alcohols is independent of GSH and enzyme activity improves with chain length.
Present with 8370 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

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