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P32771

- FADH_YEAST

UniProt

P32771 - FADH_YEAST

Protein

S-(hydroxymethyl)glutathione dehydrogenase

Gene

SFA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Oxidizes long-chain alcohols and, in the presence of glutathione, is able to oxidize formaldehyde. Is responsible for yeast resistance to formaldehyde.

    Catalytic activityi

    S-(hydroxymethyl)glutathione + NAD(P)+ = S-formylglutathione + NAD(P)H.
    An alcohol + NAD+ = an aldehyde or ketone + NADH.

    Cofactori

    Binds 2 zinc ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi49 – 491Zinc 1; catalyticBy similarity
    Metal bindingi71 – 711Zinc 1; catalyticBy similarity
    Metal bindingi101 – 1011Zinc 2By similarity
    Metal bindingi104 – 1041Zinc 2By similarity
    Metal bindingi107 – 1071Zinc 2By similarity
    Metal bindingi115 – 1151Zinc 2By similarity
    Metal bindingi179 – 1791Zinc 1; catalyticBy similarity

    GO - Molecular functioni

    1. alcohol dehydrogenase (NAD) activity Source: SGD
    2. hydroxymethylfurfural reductase (NADH) activity Source: SGD
    3. S-(hydroxymethyl)glutathione dehydrogenase activity Source: SGD
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. amino acid catabolic process to alcohol via Ehrlich pathway Source: SGD
    2. ethanol oxidation Source: InterPro
    3. formaldehyde catabolic process Source: SGD
    4. furaldehyde metabolic process Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Metal-binding, NAD, Zinc

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-11840.
    YEAST:YDL168W-MONOMER.
    ReactomeiREACT_188930. Abacavir metabolism.
    REACT_86838. Ethanol oxidation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    S-(hydroxymethyl)glutathione dehydrogenase (EC:1.1.1.284)
    Alternative name(s):
    Alcohol dehydrogenase SFA (EC:1.1.1.1)
    Glutathione-dependent formaldehyde dehydrogenase (EC:1.1.1.-)
    Short name:
    FALDH
    Short name:
    FDH
    Short name:
    FLD
    Short name:
    GSH-FDH
    Gene namesi
    Name:SFA1
    Synonyms:SFA
    Ordered Locus Names:YDL168W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDL168w.
    SGDiS000002327. SFA1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 386386S-(hydroxymethyl)glutathione dehydrogenasePRO_0000160785Add
    BLAST

    Proteomic databases

    MaxQBiP32771.
    PaxDbiP32771.
    PeptideAtlasiP32771.

    Expressioni

    Inductioni

    By formaldehyde, ethanol and methyl methanesulphonate.

    Gene expression databases

    GenevestigatoriP32771.

    Interactioni

    Protein-protein interaction databases

    BioGridi31897. 77 interactions.
    DIPiDIP-5366N.
    IntActiP32771. 2 interactions.
    MINTiMINT-539686.
    STRINGi4932.YDL168W.

    Structurei

    3D structure databases

    ProteinModelPortaliP32771.
    SMRiP32771. Positions 10-381.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1062.
    GeneTreeiENSGT00430000030800.
    HOGENOMiHOG000294674.
    KOiK00121.
    OMAiAWKSGAP.
    OrthoDBiEOG75F4PS.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.180.10. 1 hit.
    InterProiIPR014183. ADH_3.
    IPR013149. ADH_C.
    IPR013154. ADH_GroES-like.
    IPR002085. ADH_SF_Zn-type.
    IPR002328. ADH_Zn_CS.
    IPR011032. GroES-like.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11695. PTHR11695. 1 hit.
    PfamiPF08240. ADH_N. 1 hit.
    PF00107. ADH_zinc_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF50129. SSF50129. 2 hits.
    TIGRFAMsiTIGR02818. adh_III_F_hyde. 1 hit.
    PROSITEiPS00059. ADH_ZINC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32771-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAATVGKPI KCIAAVAYDA KKPLSVEEIT VDAPKAHEVR IKIEYTAVCH    50
    TDAYTLSGSD PEGLFPCVLG HEGAGIVESV GDDVITVKPG DHVIALYTAE 100
    CGKCKFCTSG KTNLCGAVRA TQGKGVMPDG TTRFHNAKGE DIYHFMGCST 150
    FSEYTVVADV SVVAIDPKAP LDAACLLGCG VTTGFGAALK TANVQKGDTV 200
    AVFGCGTVGL SVIQGAKLRG ASKIIAIDIN NKKKQYCSQF GATDFVNPKE 250
    DLAKDQTIVE KLIEMTDGGL DFTFDCTGNT KIMRDALEAC HKGWGQSIII 300
    GVAAAGEEIS TRPFQLVTGR VWKGSAFGGI KGRSEMGGLI KDYQKGALKV 350
    EEFITHRRPF KEINQAFEDL HNGDCLRTVL KSDEIK 386
    Length:386
    Mass (Da):41,042
    Last modified:October 1, 1993 - v1
    Checksum:iA495AFD7B47E4B11
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68020 Genomic DNA. Translation: CAA48161.1.
    Z67750 Genomic DNA. Translation: CAA91578.1.
    Z74216 Genomic DNA. Translation: CAA98742.1.
    BK006938 Genomic DNA. Translation: DAA11693.1.
    PIRiS31140.
    RefSeqiNP_010113.1. NM_001180228.1.

    Genome annotation databases

    EnsemblFungiiYDL168W; YDL168W; YDL168W.
    GeneIDi851386.
    KEGGisce:YDL168W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68020 Genomic DNA. Translation: CAA48161.1 .
    Z67750 Genomic DNA. Translation: CAA91578.1 .
    Z74216 Genomic DNA. Translation: CAA98742.1 .
    BK006938 Genomic DNA. Translation: DAA11693.1 .
    PIRi S31140.
    RefSeqi NP_010113.1. NM_001180228.1.

    3D structure databases

    ProteinModelPortali P32771.
    SMRi P32771. Positions 10-381.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31897. 77 interactions.
    DIPi DIP-5366N.
    IntActi P32771. 2 interactions.
    MINTi MINT-539686.
    STRINGi 4932.YDL168W.

    Proteomic databases

    MaxQBi P32771.
    PaxDbi P32771.
    PeptideAtlasi P32771.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDL168W ; YDL168W ; YDL168W .
    GeneIDi 851386.
    KEGGi sce:YDL168W.

    Organism-specific databases

    CYGDi YDL168w.
    SGDi S000002327. SFA1.

    Phylogenomic databases

    eggNOGi COG1062.
    GeneTreei ENSGT00430000030800.
    HOGENOMi HOG000294674.
    KOi K00121.
    OMAi AWKSGAP.
    OrthoDBi EOG75F4PS.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-11840.
    YEAST:YDL168W-MONOMER.
    Reactomei REACT_188930. Abacavir metabolism.
    REACT_86838. Ethanol oxidation.

    Miscellaneous databases

    NextBioi 968534.
    PROi P32771.

    Gene expression databases

    Genevestigatori P32771.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.180.10. 1 hit.
    InterProi IPR014183. ADH_3.
    IPR013149. ADH_C.
    IPR013154. ADH_GroES-like.
    IPR002085. ADH_SF_Zn-type.
    IPR002328. ADH_Zn_CS.
    IPR011032. GroES-like.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11695. PTHR11695. 1 hit.
    Pfami PF08240. ADH_N. 1 hit.
    PF00107. ADH_zinc_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50129. SSF50129. 2 hits.
    TIGRFAMsi TIGR02818. adh_III_F_hyde. 1 hit.
    PROSITEi PS00059. ADH_ZINC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular structure and genetic regulation of SFA, a gene responsible for resistance to formaldehyde in Saccharomyces cerevisiae, and characterization of its protein product."
      Wehner E.P., Rao E., Brendel M.
      Mol. Gen. Genet. 237:351-358(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 38626 / AH22 / NRRL Y-12843.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiFADH_YEAST
    AccessioniPrimary (citable) accession number: P32771
    Secondary accession number(s): D6VRI3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 132 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Requires GSH for oxidation of formaldehyde or of methylglyoxal, while oxidation of long-chain alcohols is independent of GSH and enzyme activity improves with chain length.
    Present with 8370 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3