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Protein

S-(hydroxymethyl)glutathione dehydrogenase

Gene

SFA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Oxidizes long-chain alcohols and, in the presence of glutathione, is able to oxidize formaldehyde. Is responsible for yeast resistance to formaldehyde.

Miscellaneous

Requires GSH for oxidation of formaldehyde or of methylglyoxal, while oxidation of long-chain alcohols is independent of GSH and enzyme activity improves with chain length.
Present with 8370 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

S-(hydroxymethyl)glutathione + NAD(P)+ = S-formylglutathione + NAD(P)H.
A primary alcohol + NAD+ = an aldehyde + NADH.
A secondary alcohol + NAD+ = a ketone + NADH.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi49Zinc 1; catalyticBy similarity1
Metal bindingi71Zinc 1; catalyticBy similarity1
Metal bindingi101Zinc 2By similarity1
Metal bindingi104Zinc 2By similarity1
Metal bindingi107Zinc 2By similarity1
Metal bindingi115Zinc 2By similarity1
Metal bindingi179Zinc 1; catalyticBy similarity1

GO - Molecular functioni

  • alcohol dehydrogenase (NAD) activity Source: SGD
  • hydroxymethylfurfural reductase (NADH) activity Source: SGD
  • S-(hydroxymethyl)glutathione dehydrogenase activity Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • amino acid catabolic process to alcohol via Ehrlich pathway Source: SGD
  • ethanol oxidation Source: InterPro
  • formaldehyde catabolic process Source: SGD
  • furaldehyde metabolic process Source: SGD

Keywordsi

Molecular functionOxidoreductase
LigandMetal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:YDL168W-MONOMER
YEAST:YDL168W-MONOMER
ReactomeiR-SCE-2161541 Abacavir metabolism
R-SCE-5365859 RA biosynthesis pathway
R-SCE-71384 Ethanol oxidation

Names & Taxonomyi

Protein namesi
Recommended name:
S-(hydroxymethyl)glutathione dehydrogenase (EC:1.1.1.284)
Alternative name(s):
Alcohol dehydrogenase SFA (EC:1.1.1.1)
Glutathione-dependent formaldehyde dehydrogenase (EC:1.1.1.-)
Short name:
FALDH
Short name:
FDH
Short name:
FLD
Short name:
GSH-FDH
Gene namesi
Name:SFA1
Synonyms:SFA
Ordered Locus Names:YDL168W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL168W
SGDiS000002327 SFA1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001607851 – 386S-(hydroxymethyl)glutathione dehydrogenaseAdd BLAST386

Proteomic databases

MaxQBiP32771
PaxDbiP32771
PRIDEiP32771

Expressioni

Inductioni

By formaldehyde, ethanol and methyl methanesulphonate.

Interactioni

Protein-protein interaction databases

BioGridi31897, 158 interactors
DIPiDIP-5366N
IntActiP32771, 1 interactor
MINTiP32771
STRINGi4932.YDL168W

Structurei

3D structure databases

ProteinModelPortaliP32771
SMRiP32771
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00430000030800
HOGENOMiHOG000294674
InParanoidiP32771
KOiK00121
OMAiCIGNVNT
OrthoDBiEOG092C2RA1

Family and domain databases

CDDicd08300 alcohol_DH_class_III, 1 hit
InterProiView protein in InterPro
IPR014183 ADH_3
IPR013149 ADH_C
IPR013154 ADH_N
IPR002328 ADH_Zn_CS
IPR011032 GroES-like_sf
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF08240 ADH_N, 1 hit
PF00107 ADH_zinc_N, 1 hit
SUPFAMiSSF50129 SSF50129, 2 hits
SSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR02818 adh_III_F_hyde, 1 hit
PROSITEiView protein in PROSITE
PS00059 ADH_ZINC, 1 hit

Sequencei

Sequence statusi: Complete.

P32771-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAATVGKPI KCIAAVAYDA KKPLSVEEIT VDAPKAHEVR IKIEYTAVCH
60 70 80 90 100
TDAYTLSGSD PEGLFPCVLG HEGAGIVESV GDDVITVKPG DHVIALYTAE
110 120 130 140 150
CGKCKFCTSG KTNLCGAVRA TQGKGVMPDG TTRFHNAKGE DIYHFMGCST
160 170 180 190 200
FSEYTVVADV SVVAIDPKAP LDAACLLGCG VTTGFGAALK TANVQKGDTV
210 220 230 240 250
AVFGCGTVGL SVIQGAKLRG ASKIIAIDIN NKKKQYCSQF GATDFVNPKE
260 270 280 290 300
DLAKDQTIVE KLIEMTDGGL DFTFDCTGNT KIMRDALEAC HKGWGQSIII
310 320 330 340 350
GVAAAGEEIS TRPFQLVTGR VWKGSAFGGI KGRSEMGGLI KDYQKGALKV
360 370 380
EEFITHRRPF KEINQAFEDL HNGDCLRTVL KSDEIK
Length:386
Mass (Da):41,042
Last modified:October 1, 1993 - v1
Checksum:iA495AFD7B47E4B11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68020 Genomic DNA Translation: CAA48161.1
Z67750 Genomic DNA Translation: CAA91578.1
Z74216 Genomic DNA Translation: CAA98742.1
BK006938 Genomic DNA Translation: DAA11693.1
PIRiS31140
RefSeqiNP_010113.1, NM_001180228.1

Genome annotation databases

EnsemblFungiiYDL168W; YDL168W; YDL168W
GeneIDi851386
KEGGisce:YDL168W

Similar proteinsi

Entry informationi

Entry nameiFADH_YEAST
AccessioniPrimary (citable) accession number: P32771
Secondary accession number(s): D6VRI3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 23, 2018
This is version 162 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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