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P32770

- NRP1_YEAST

UniProt

P32770 - NRP1_YEAST

Protein

Asparagine-rich protein

Gene

NRP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri355 – 38430RanBP2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri581 – 61030RanBP2-type 2PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. nucleotide binding Source: InterPro
    2. RNA binding Source: UniProtKB-KW
    3. zinc ion binding Source: InterPro

    Keywords - Ligandi

    Metal-binding, RNA-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29559-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Asparagine-rich protein
    Short name:
    Protein ARP
    Gene namesi
    Name:NRP1
    Synonyms:ARP, ARP1
    Ordered Locus Names:YDL167C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDL167c.
    SGDiS000002326. NRP1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasmic stress granule Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 719719Asparagine-rich proteinPRO_0000081689Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei345 – 3451Phosphoserine2 Publications
    Modified residuei455 – 4551Phosphoserine2 Publications
    Modified residuei630 – 6301Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP32770.
    PaxDbiP32770.
    PeptideAtlasiP32770.
    PRIDEiP32770.

    Expressioni

    Gene expression databases

    GenevestigatoriP32770.

    Interactioni

    Protein-protein interaction databases

    BioGridi31898. 45 interactions.
    DIPiDIP-5159N.
    IntActiP32770. 13 interactions.
    MINTiMINT-513079.
    STRINGi4932.YDL167C.

    Structurei

    3D structure databases

    ProteinModelPortaliP32770.
    SMRiP32770. Positions 226-310, 349-385, 581-609.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini226 – 32297RRMPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi490 – 56475Asn-richAdd
    BLAST

    Sequence similaritiesi

    Contains 2 RanBP2-type zinc fingers.PROSITE-ProRule annotation
    Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri355 – 38430RanBP2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri581 – 61030RanBP2-type 2PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG317445.
    OMAiANTKQPR.
    OrthoDBiEOG7NPG2Z.

    Family and domain databases

    Gene3Di3.30.70.330. 1 hit.
    4.10.1060.10. 2 hits.
    InterProiIPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    IPR001876. Znf_RanBP2.
    [Graphical view]
    PfamiPF00641. zf-RanBP. 2 hits.
    [Graphical view]
    SMARTiSM00360. RRM. 1 hit.
    SM00547. ZnF_RBZ. 2 hits.
    [Graphical view]
    PROSITEiPS50102. RRM. 1 hit.
    PS01358. ZF_RANBP2_1. 2 hits.
    PS50199. ZF_RANBP2_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32770-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHYVVLELQV AHLPDTPKDQ CRIANIAFQI VNAETLVCHY GTNSLPSIEV    50
    NGTTKSLESA MVQLDKDIHD VIGNDDFVLV SLYSTWHIRV TLPRQARDDG 100
    FILTSYLQHP KVFDLWKEFD RWCVNHPEIL GQKKAISNNN CNTKSISINA 150
    AKNTKDLDEI VRILEVSIPT EEAGSVPEIY SLLKRTTDIL IQLHKKCTSP 200
    EDMESVLTKP YDSHTDIRAF LQEKSKILYM NNLPPDTTQS ELESWFTQYG 250
    VRPVGFWTVK NIVEDTSNVN NNWSLNNSPY VEDQDSISGF VVFQTHEEAT 300
    EVLALNGRSI LSNLANTKQP RVVEHVLELQ PSSTGVLDKA QEILSPFPQS 350
    KNKPRPGDWN CPSCGFSNFQ RRTACFRCSF PAPSNSQIHT ANSNNNVNSS 400
    RNNLNNRVNS GSSSNISNTA ANHPYGAPEF NMIANNTPAA LTYNRAHFPA 450
    ITPLSRQNSL NMAPSNSGSP IIIADHFSGN NNIAPNYRYN NNINNNNNNI 500
    NNMTNNRYNI NNNINGNGNG NGNNSNNNNN HNNNHNNNHH NGSINSNSNT 550
    NNNNNNNNGN NSNNCNSNIG MGGCGSNMPF RAGDWKCSTC TYHNFAKNVV 600
    CLRCGGPKSI SGDASETNHY IDSSTFGPAS RTPSNNNISV NTNGGSNAGR 650
    TDGNDNKGRD ISLMEFMSPP LSMATKSMKE GDGNGSSFNE FKSDKANVNF 700
    SNVGDNSAFG NGFNSSIRW 719
    Length:719
    Mass (Da):79,299
    Last modified:November 1, 1997 - v2
    Checksum:iADA9BC09FD582669
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti493 – 4931I → N in CAA48159. (PubMed:8483449)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68020 Genomic DNA. Translation: CAA48159.1.
    Z67750 Genomic DNA. Translation: CAA91579.1.
    Z74215 Genomic DNA. Translation: CAA98741.1.
    BK006938 Genomic DNA. Translation: DAA11694.1.
    PIRiS61046.
    RefSeqiNP_010114.1. NM_001180227.1.

    Genome annotation databases

    EnsemblFungiiYDL167C; YDL167C; YDL167C.
    GeneIDi851387.
    KEGGisce:YDL167C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68020 Genomic DNA. Translation: CAA48159.1 .
    Z67750 Genomic DNA. Translation: CAA91579.1 .
    Z74215 Genomic DNA. Translation: CAA98741.1 .
    BK006938 Genomic DNA. Translation: DAA11694.1 .
    PIRi S61046.
    RefSeqi NP_010114.1. NM_001180227.1.

    3D structure databases

    ProteinModelPortali P32770.
    SMRi P32770. Positions 226-310, 349-385, 581-609.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31898. 45 interactions.
    DIPi DIP-5159N.
    IntActi P32770. 13 interactions.
    MINTi MINT-513079.
    STRINGi 4932.YDL167C.

    Proteomic databases

    MaxQBi P32770.
    PaxDbi P32770.
    PeptideAtlasi P32770.
    PRIDEi P32770.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDL167C ; YDL167C ; YDL167C .
    GeneIDi 851387.
    KEGGi sce:YDL167C.

    Organism-specific databases

    CYGDi YDL167c.
    SGDi S000002326. NRP1.

    Phylogenomic databases

    eggNOGi NOG317445.
    OMAi ANTKQPR.
    OrthoDBi EOG7NPG2Z.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29559-MONOMER.

    Miscellaneous databases

    NextBioi 968537.

    Gene expression databases

    Genevestigatori P32770.

    Family and domain databases

    Gene3Di 3.30.70.330. 1 hit.
    4.10.1060.10. 2 hits.
    InterProi IPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    IPR001876. Znf_RanBP2.
    [Graphical view ]
    Pfami PF00641. zf-RanBP. 2 hits.
    [Graphical view ]
    SMARTi SM00360. RRM. 1 hit.
    SM00547. ZnF_RBZ. 2 hits.
    [Graphical view ]
    PROSITEi PS50102. RRM. 1 hit.
    PS01358. ZF_RANBP2_1. 2 hits.
    PS50199. ZF_RANBP2_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular structure and genetic regulation of SFA, a gene responsible for resistance to formaldehyde in Saccharomyces cerevisiae, and characterization of its protein product."
      Wehner E.P., Rao E., Brendel M.
      Mol. Gen. Genet. 237:351-358(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 38626 / AH22 / NRRL Y-12843.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-345, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-455, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-345 AND SER-455, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-630, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNRP1_YEAST
    AccessioniPrimary (citable) accession number: P32770
    Secondary accession number(s): D6VRI4, Q12228
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 130 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3570 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3