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Protein

Elongation factor 1 alpha-like protein

Gene

HBS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in protein translation. Together with DOM34, may function in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi174 – 181GTPBy similarity8
Nucleotide bindingi251 – 255GTPBy similarity5
Nucleotide bindingi313 – 316GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW
  • translation elongation factor activity Source: UniProtKB-KW

GO - Biological processi

  • nonfunctional rRNA decay Source: SGD
  • nuclear-transcribed mRNA catabolic process, no-go decay Source: SGD
  • positive regulation of translation Source: SGD
  • ribosome disassembly Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis, Translation regulation

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32047-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 1 alpha-like protein
Gene namesi
Name:HBS1
Ordered Locus Names:YKR084C
ORF Names:YKR404
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKR084C.
SGDiS000001792. HBS1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • Dom34-Hbs1 complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi176V → G: Loss of function. 1 Publication1
Mutagenesisi255H → E: Loss of function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000914901 – 611Elongation factor 1 alpha-like proteinAdd BLAST611

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei124PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32769.
PRIDEiP32769.

PTM databases

iPTMnetiP32769.

Interactioni

Subunit structurei

Interacts with DOM34.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
DOM34P333093EBI-8194,EBI-6012

Protein-protein interaction databases

BioGridi34215. 36 interactors.
DIPiDIP-762N.
IntActiP32769. 1 interactor.
MINTiMINT-4493888.

Structurei

Secondary structure

1611
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi157 – 163Combined sources7
Beta strandi167 – 174Combined sources8
Helixi176 – 178Combined sources3
Helixi180 – 190Combined sources11
Helixi196 – 202Combined sources7
Helixi219 – 222Combined sources4
Beta strandi237 – 241Combined sources5
Beta strandi246 – 250Combined sources5
Helixi256 – 258Combined sources3
Helixi259 – 266Combined sources8
Beta strandi270 – 277Combined sources8
Helixi291 – 302Combined sources12
Beta strandi308 – 313Combined sources6
Helixi315 – 318Combined sources4
Helixi322 – 339Combined sources18
Helixi343 – 345Combined sources3
Beta strandi346 – 350Combined sources5
Beta strandi353 – 355Combined sources3
Beta strandi359 – 362Combined sources4
Helixi366 – 371Combined sources6
Helixi377 – 392Combined sources16
Beta strandi397 – 399Combined sources3
Beta strandi402 – 409Combined sources8
Beta strandi419 – 430Combined sources12
Beta strandi435 – 439Combined sources5
Turni440 – 442Combined sources3
Beta strandi443 – 452Combined sources10
Turni453 – 455Combined sources3
Beta strandi464 – 466Combined sources3
Beta strandi473 – 480Combined sources8
Helixi483 – 485Combined sources3
Beta strandi491 – 493Combined sources3
Beta strandi504 – 512Combined sources9
Beta strandi524 – 529Combined sources6
Beta strandi532 – 547Combined sources16
Beta strandi564 – 571Combined sources8
Turni583 – 585Combined sources3
Turni587 – 590Combined sources4
Beta strandi591 – 596Combined sources6
Beta strandi599 – 609Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3IZQelectron microscopy-11-611[»]
3P26X-ray2.50A/B135-611[»]
3P27X-ray2.95A/B135-611[»]
ProteinModelPortaliP32769.
SMRiP32769.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini165 – 390tr-type GPROSITE-ProRule annotationAdd BLAST226

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni174 – 181G1PROSITE-ProRule annotation8
Regioni230 – 234G2PROSITE-ProRule annotation5
Regioni251 – 254G3PROSITE-ProRule annotation4
Regioni313 – 316G4PROSITE-ProRule annotation4
Regioni352 – 354G5PROSITE-ProRule annotation3

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family.PROSITE-ProRule annotation
Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00600000085333.
HOGENOMiHOG000229291.
InParanoidiP32769.
KOiK14416.
OMAiDYQGWDN.
OrthoDBiEOG092C2HV8.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR031157. G_TR_CS.
IPR015033. HBS1-like_N.
IPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03143. GTP_EFTU_D3. 1 hit.
PF08938. HBS1_N. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32769-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYSDYSDGA DDMPDFHDEG EFDDYLNDDE YDLMNEVFPT LKAQLQDYQG
60 70 80 90 100
WDNLSLKLAL FDNNFDLEST LAELKKTLKK KKTPKKPIAA ANGSANVTQK
110 120 130 140 150
LANISISQQR PNDRLPDWLD EEESEGERNG EEANDEKTVQ RYYKTTVPTK
160 170 180 190 200
PKKPHDISAF VKSALPHLSF VVLGHVDAGK STLMGRLLYD LNIVNQSQLR
210 220 230 240 250
KLQRESETMG KSSFKFAWIM DQTNEERERG VTVSICTSHF STHRANFTIV
260 270 280 290 300
DAPGHRDFVP NAIMGISQAD MAILCVDCST NAFESGFDLD GQTKEHMLLA
310 320 330 340 350
SSLGIHNLII AMNKMDNVDW SQQRFEEIKS KLLPYLVDIG FFEDNINWVP
360 370 380 390 400
ISGFSGEGVY KIEYTDEVRQ WYNGPNLMST LENAAFKISK ENEGINKDDP
410 420 430 440 450
FLFSVLEIIP SKKTSNDLAL VSGKLESGSI QPGESLTIYP SEQSCIVDKI
460 470 480 490 500
QVGSQQGQST NHEETDVAIK GDFVTLKLRK AYPEDIQNGD LAASVDYSSI
510 520 530 540 550
HSAQCFVLEL TTFDMNRPLL PGTPFILFIG VKEQPARIKR LISFIDKGNT
560 570 580 590 600
ASKKKIRHLG SKQRAFVEIE LIEVKRWIPL LTAHENDRLG RVVLRKDGRT
610
IAAGKISEIT Q
Length:611
Mass (Da):68,730
Last modified:June 1, 1994 - v2
Checksum:i11EC2CB6936396EE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti92 – 94NGS → MGV in M98437 (PubMed:1394434).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98437 Genomic DNA. No translation available.
Z27116 Genomic DNA. Translation: CAA81635.1.
Z28309 Genomic DNA. Translation: CAA82163.1.
BK006944 Genomic DNA. Translation: DAA09234.1.
PIRiS38162.
RefSeqiNP_013010.3. NM_001179874.3.

Genome annotation databases

EnsemblFungiiYKR084C; YKR084C; YKR084C.
GeneIDi853959.
KEGGisce:YKR084C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98437 Genomic DNA. No translation available.
Z27116 Genomic DNA. Translation: CAA81635.1.
Z28309 Genomic DNA. Translation: CAA82163.1.
BK006944 Genomic DNA. Translation: DAA09234.1.
PIRiS38162.
RefSeqiNP_013010.3. NM_001179874.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3IZQelectron microscopy-11-611[»]
3P26X-ray2.50A/B135-611[»]
3P27X-ray2.95A/B135-611[»]
ProteinModelPortaliP32769.
SMRiP32769.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34215. 36 interactors.
DIPiDIP-762N.
IntActiP32769. 1 interactor.
MINTiMINT-4493888.

PTM databases

iPTMnetiP32769.

Proteomic databases

MaxQBiP32769.
PRIDEiP32769.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKR084C; YKR084C; YKR084C.
GeneIDi853959.
KEGGisce:YKR084C.

Organism-specific databases

EuPathDBiFungiDB:YKR084C.
SGDiS000001792. HBS1.

Phylogenomic databases

GeneTreeiENSGT00600000085333.
HOGENOMiHOG000229291.
InParanoidiP32769.
KOiK14416.
OMAiDYQGWDN.
OrthoDBiEOG092C2HV8.

Enzyme and pathway databases

BioCyciYEAST:G3O-32047-MONOMER.

Miscellaneous databases

PROiP32769.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR031157. G_TR_CS.
IPR015033. HBS1-like_N.
IPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03143. GTP_EFTU_D3. 1 hit.
PF08938. HBS1_N. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHBS1_YEAST
AccessioniPrimary (citable) accession number: P32769
Secondary accession number(s): D6VXE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: June 1, 1994
Last modified: November 2, 2016
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2750 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.