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P32769 (HBS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Elongation factor 1 alpha-like protein
Gene names
Name:HBS1
Ordered Locus Names:YKR084C
ORF Names:YKR404
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length611 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in protein translation. Together with DOM34, may function in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs. Ref.8

Subunit structure

Interacts with DOM34. Ref.5

Subcellular location

Cytoplasm Ref.6.

Miscellaneous

Present with 2750 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the GTP-binding elongation factor family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

DOM34P333093EBI-8194,EBI-6012

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 611611Elongation factor 1 alpha-like protein
PRO_0000091490

Regions

Nucleotide binding174 – 1818GTP By similarity
Nucleotide binding251 – 2555GTP By similarity
Nucleotide binding313 – 3164GTP By similarity

Amino acid modifications

Modified residue1241Phosphoserine Ref.9 Ref.10 Ref.11

Experimental info

Mutagenesis1761V → G: Loss of function. Ref.5
Mutagenesis2551H → E: Loss of function. Ref.5
Sequence conflict92 – 943NGS → MGV in M98437. Ref.4

Secondary structure

........................................................................... 611
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P32769 [UniParc].

Last modified June 1, 1994. Version 2.
Checksum: 11EC2CB6936396EE

FASTA61168,730
        10         20         30         40         50         60 
MAYSDYSDGA DDMPDFHDEG EFDDYLNDDE YDLMNEVFPT LKAQLQDYQG WDNLSLKLAL 

        70         80         90        100        110        120 
FDNNFDLEST LAELKKTLKK KKTPKKPIAA ANGSANVTQK LANISISQQR PNDRLPDWLD 

       130        140        150        160        170        180 
EEESEGERNG EEANDEKTVQ RYYKTTVPTK PKKPHDISAF VKSALPHLSF VVLGHVDAGK 

       190        200        210        220        230        240 
STLMGRLLYD LNIVNQSQLR KLQRESETMG KSSFKFAWIM DQTNEERERG VTVSICTSHF 

       250        260        270        280        290        300 
STHRANFTIV DAPGHRDFVP NAIMGISQAD MAILCVDCST NAFESGFDLD GQTKEHMLLA 

       310        320        330        340        350        360 
SSLGIHNLII AMNKMDNVDW SQQRFEEIKS KLLPYLVDIG FFEDNINWVP ISGFSGEGVY 

       370        380        390        400        410        420 
KIEYTDEVRQ WYNGPNLMST LENAAFKISK ENEGINKDDP FLFSVLEIIP SKKTSNDLAL 

       430        440        450        460        470        480 
VSGKLESGSI QPGESLTIYP SEQSCIVDKI QVGSQQGQST NHEETDVAIK GDFVTLKLRK 

       490        500        510        520        530        540 
AYPEDIQNGD LAASVDYSSI HSAQCFVLEL TTFDMNRPLL PGTPFILFIG VKEQPARIKR 

       550        560        570        580        590        600 
LISFIDKGNT ASKKKIRHLG SKQRAFVEIE LIEVKRWIPL LTAHENDRLG RVVLRKDGRT 

       610 
IAAGKISEIT Q 

« Hide

References

« Hide 'large scale' references
[1]"The complete sequence of an 18,002 bp segment of Saccharomyces cerevisiae chromosome XI contains the HBS1, MRP-L20 and PRP16 genes, and six new open reading frames."
Garcia-Cantalejo J.M., Baladron V., Esteban P.F., Santos M.A., Bou G., Remacha M.A., Revuelta J.L., Ballesta J.P.G., Jimenez A., del Rey F.
Yeast 10:231-245(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"The translation machinery and 70 kd heat shock protein cooperate in protein synthesis."
Nelson R.J., Ziegelhoffer T., Nicolet C., Werner-Washburne M., Craig E.A.
Cell 71:97-105(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 92-611.
[5]"Novel G-protein complex whose requirement is linked to the translational status of the cell."
Carr-Schmid A., Pfund C., Craig E.A., Kinzy T.G.
Mol. Cell. Biol. 22:2564-2574(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DOM34, MUTAGENESIS OF VAL-176 AND HIS-255.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Endonucleolytic cleavage of eukaryotic mRNAs with stalls in translation elongation."
Doma M.K., Parker R.
Nature 440:561-564(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-124, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-124, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-124, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M98437 Genomic DNA. No translation available.
Z27116 Genomic DNA. Translation: CAA81635.1.
Z28309 Genomic DNA. Translation: CAA82163.1.
BK006944 Genomic DNA. Translation: DAA09234.1.
PIRS38162.
RefSeqNP_013010.3. NM_001179874.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3IZQelectron microscopy-11-611[»]
3P26X-ray2.50A/B135-611[»]
3P27X-ray2.95A/B135-611[»]
ProteinModelPortalP32769.
SMRP32769. Positions 20-86, 161-611.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34215. 34 interactions.
DIPDIP-762N.
IntActP32769. 1 interaction.
MINTMINT-4493888.
STRING4932.YKR084C.

Proteomic databases

MaxQBP32769.
PaxDbP32769.
PeptideAtlasP32769.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKR084C; YKR084C; YKR084C.
GeneID853959.
KEGGsce:YKR084C.

Organism-specific databases

CYGDYKR084c.
SGDS000001792. HBS1.

Phylogenomic databases

eggNOGCOG5256.
GeneTreeENSGT00600000085333.
HOGENOMHOG000229291.
KOK14416.
OMAICATDFE.
OrthoDBEOG715QCW.

Enzyme and pathway databases

BioCycYEAST:G3O-32047-MONOMER.

Gene expression databases

GenevestigatorP32769.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR000795. EF_GTP-bd_dom.
IPR015033. HBS1-like_N.
IPR027417. P-loop_NTPase.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF08938. HBS1_N. 1 hit.
[Graphical view]
PRINTSPR00315. ELONGATNFCT.
SUPFAMSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio975380.

Entry information

Entry nameHBS1_YEAST
AccessionPrimary (citable) accession number: P32769
Secondary accession number(s): D6VXE4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: June 1, 1994
Last modified: June 11, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references