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P32769

- HBS1_YEAST

UniProt

P32769 - HBS1_YEAST

Protein

Elongation factor 1 alpha-like protein

Gene

HBS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 2 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Involved in protein translation. Together with DOM34, may function in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi174 – 1818GTPBy similarity
    Nucleotide bindingi251 – 2555GTPBy similarity
    Nucleotide bindingi313 – 3164GTPBy similarity

    GO - Molecular functioni

    1. GTPase activity Source: SGD
    2. GTP binding Source: UniProtKB-KW
    3. protein binding Source: IntAct
    4. translation elongation factor activity Source: UniProtKB-KW

    GO - Biological processi

    1. GTP catabolic process Source: GOC
    2. nonfunctional rRNA decay Source: SGD
    3. nuclear-transcribed mRNA catabolic process, no-go decay Source: SGD
    4. positive regulation of translation Source: SGD
    5. ribosome disassembly Source: SGD

    Keywords - Molecular functioni

    Elongation factor

    Keywords - Biological processi

    Protein biosynthesis, Translation regulation

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32047-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Elongation factor 1 alpha-like protein
    Gene namesi
    Name:HBS1
    Ordered Locus Names:YKR084C
    ORF Names:YKR404
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKR084c.
    SGDiS000001792. HBS1.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi176 – 1761V → G: Loss of function. 1 Publication
    Mutagenesisi255 – 2551H → E: Loss of function. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 611611Elongation factor 1 alpha-like proteinPRO_0000091490Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei124 – 1241Phosphoserine3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP32769.
    PaxDbiP32769.
    PeptideAtlasiP32769.

    Expressioni

    Gene expression databases

    GenevestigatoriP32769.

    Interactioni

    Subunit structurei

    Interacts with DOM34.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    DOM34P333093EBI-8194,EBI-6012

    Protein-protein interaction databases

    BioGridi34215. 34 interactions.
    DIPiDIP-762N.
    IntActiP32769. 1 interaction.
    MINTiMINT-4493888.
    STRINGi4932.YKR084C.

    Structurei

    Secondary structure

    1
    611
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi157 – 1637
    Beta strandi167 – 1748
    Helixi176 – 1783
    Helixi180 – 19011
    Helixi196 – 2027
    Helixi219 – 2224
    Beta strandi237 – 2415
    Beta strandi246 – 2505
    Helixi256 – 2583
    Helixi259 – 2668
    Beta strandi270 – 2778
    Helixi291 – 30212
    Beta strandi308 – 3136
    Helixi315 – 3184
    Helixi322 – 33918
    Helixi343 – 3453
    Beta strandi346 – 3505
    Beta strandi353 – 3553
    Beta strandi359 – 3624
    Helixi366 – 3716
    Helixi377 – 39216
    Beta strandi397 – 3993
    Beta strandi402 – 4098
    Beta strandi419 – 43012
    Beta strandi435 – 4395
    Turni440 – 4423
    Beta strandi443 – 45210
    Turni453 – 4553
    Beta strandi464 – 4663
    Beta strandi473 – 4808
    Helixi483 – 4853
    Beta strandi491 – 4933
    Beta strandi504 – 5129
    Beta strandi524 – 5296
    Beta strandi532 – 54716
    Beta strandi564 – 5718
    Turni583 – 5853
    Turni587 – 5904
    Beta strandi591 – 5966
    Beta strandi599 – 60911

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3IZQelectron microscopy-11-611[»]
    3P26X-ray2.50A/B135-611[»]
    3P27X-ray2.95A/B135-611[»]
    ProteinModelPortaliP32769.
    SMRiP32769. Positions 20-86, 161-611.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini165 – 390226tr-type GPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni174 – 1818G1PROSITE-ProRule annotation
    Regioni230 – 2345G2PROSITE-ProRule annotation
    Regioni251 – 2544G3PROSITE-ProRule annotation
    Regioni313 – 3164G4PROSITE-ProRule annotation
    Regioni352 – 3543G5PROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family.PROSITE-ProRule annotation
    Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5256.
    GeneTreeiENSGT00600000085333.
    HOGENOMiHOG000229291.
    KOiK14416.
    OMAiICATDFE.
    OrthoDBiEOG715QCW.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR000795. EF_GTP-bd_dom.
    IPR015033. HBS1-like_N.
    IPR027417. P-loop_NTPase.
    IPR009000. Transl_B-barrel.
    IPR009001. Transl_elong_EF1A/Init_IF2_C.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view]
    PfamiPF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF08938. HBS1_N. 1 hit.
    [Graphical view]
    PRINTSiPR00315. ELONGATNFCT.
    SUPFAMiSSF50447. SSF50447. 1 hit.
    SSF50465. SSF50465. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEiPS00301. G_TR_1. 1 hit.
    PS51722. G_TR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32769-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAYSDYSDGA DDMPDFHDEG EFDDYLNDDE YDLMNEVFPT LKAQLQDYQG    50
    WDNLSLKLAL FDNNFDLEST LAELKKTLKK KKTPKKPIAA ANGSANVTQK 100
    LANISISQQR PNDRLPDWLD EEESEGERNG EEANDEKTVQ RYYKTTVPTK 150
    PKKPHDISAF VKSALPHLSF VVLGHVDAGK STLMGRLLYD LNIVNQSQLR 200
    KLQRESETMG KSSFKFAWIM DQTNEERERG VTVSICTSHF STHRANFTIV 250
    DAPGHRDFVP NAIMGISQAD MAILCVDCST NAFESGFDLD GQTKEHMLLA 300
    SSLGIHNLII AMNKMDNVDW SQQRFEEIKS KLLPYLVDIG FFEDNINWVP 350
    ISGFSGEGVY KIEYTDEVRQ WYNGPNLMST LENAAFKISK ENEGINKDDP 400
    FLFSVLEIIP SKKTSNDLAL VSGKLESGSI QPGESLTIYP SEQSCIVDKI 450
    QVGSQQGQST NHEETDVAIK GDFVTLKLRK AYPEDIQNGD LAASVDYSSI 500
    HSAQCFVLEL TTFDMNRPLL PGTPFILFIG VKEQPARIKR LISFIDKGNT 550
    ASKKKIRHLG SKQRAFVEIE LIEVKRWIPL LTAHENDRLG RVVLRKDGRT 600
    IAAGKISEIT Q 611
    Length:611
    Mass (Da):68,730
    Last modified:June 1, 1994 - v2
    Checksum:i11EC2CB6936396EE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti92 – 943NGS → MGV in M98437. (PubMed:1394434)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M98437 Genomic DNA. No translation available.
    Z27116 Genomic DNA. Translation: CAA81635.1.
    Z28309 Genomic DNA. Translation: CAA82163.1.
    BK006944 Genomic DNA. Translation: DAA09234.1.
    PIRiS38162.
    RefSeqiNP_013010.3. NM_001179874.3.

    Genome annotation databases

    EnsemblFungiiYKR084C; YKR084C; YKR084C.
    GeneIDi853959.
    KEGGisce:YKR084C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M98437 Genomic DNA. No translation available.
    Z27116 Genomic DNA. Translation: CAA81635.1 .
    Z28309 Genomic DNA. Translation: CAA82163.1 .
    BK006944 Genomic DNA. Translation: DAA09234.1 .
    PIRi S38162.
    RefSeqi NP_013010.3. NM_001179874.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3IZQ electron microscopy - 1 1-611 [» ]
    3P26 X-ray 2.50 A/B 135-611 [» ]
    3P27 X-ray 2.95 A/B 135-611 [» ]
    ProteinModelPortali P32769.
    SMRi P32769. Positions 20-86, 161-611.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34215. 34 interactions.
    DIPi DIP-762N.
    IntActi P32769. 1 interaction.
    MINTi MINT-4493888.
    STRINGi 4932.YKR084C.

    Proteomic databases

    MaxQBi P32769.
    PaxDbi P32769.
    PeptideAtlasi P32769.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKR084C ; YKR084C ; YKR084C .
    GeneIDi 853959.
    KEGGi sce:YKR084C.

    Organism-specific databases

    CYGDi YKR084c.
    SGDi S000001792. HBS1.

    Phylogenomic databases

    eggNOGi COG5256.
    GeneTreei ENSGT00600000085333.
    HOGENOMi HOG000229291.
    KOi K14416.
    OMAi ICATDFE.
    OrthoDBi EOG715QCW.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32047-MONOMER.

    Miscellaneous databases

    NextBioi 975380.

    Gene expression databases

    Genevestigatori P32769.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR000795. EF_GTP-bd_dom.
    IPR015033. HBS1-like_N.
    IPR027417. P-loop_NTPase.
    IPR009000. Transl_B-barrel.
    IPR009001. Transl_elong_EF1A/Init_IF2_C.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view ]
    Pfami PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF08938. HBS1_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00315. ELONGATNFCT.
    SUPFAMi SSF50447. SSF50447. 1 hit.
    SSF50465. SSF50465. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEi PS00301. G_TR_1. 1 hit.
    PS51722. G_TR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete sequence of an 18,002 bp segment of Saccharomyces cerevisiae chromosome XI contains the HBS1, MRP-L20 and PRP16 genes, and six new open reading frames."
      Garcia-Cantalejo J.M., Baladron V., Esteban P.F., Santos M.A., Bou G., Remacha M.A., Revuelta J.L., Ballesta J.P.G., Jimenez A., del Rey F.
      Yeast 10:231-245(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The translation machinery and 70 kd heat shock protein cooperate in protein synthesis."
      Nelson R.J., Ziegelhoffer T., Nicolet C., Werner-Washburne M., Craig E.A.
      Cell 71:97-105(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 92-611.
    5. "Novel G-protein complex whose requirement is linked to the translational status of the cell."
      Carr-Schmid A., Pfund C., Craig E.A., Kinzy T.G.
      Mol. Cell. Biol. 22:2564-2574(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DOM34, MUTAGENESIS OF VAL-176 AND HIS-255.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Endonucleolytic cleavage of eukaryotic mRNAs with stalls in translation elongation."
      Doma M.K., Parker R.
      Nature 440:561-564(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-124, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-124, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-124, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiHBS1_YEAST
    AccessioniPrimary (citable) accession number: P32769
    Secondary accession number(s): D6VXE4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 127 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2750 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3