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Protein

Flocculation protein FLO1

Gene

FLO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell wall protein that participates directly in adhesive cell-cell interactions during yeast flocculation, a reversible, asexual and Ca2+-dependent process in which cells adhere to form aggregates (flocs) consisting of thousands of cells. The lectin-like protein sticks out of the cell wall of flocculent cells and selectively binds mannose residues in the cell walls of adjacent cells. Activity is inhibited by mannose, but not by glucose, maltose, sucrose or galactose. Also involved in cell-substrate adhesion.4 Publications

GO - Molecular functioni

  • mannose binding Source: SGD

GO - Biological processi

  • cellular response to ethanol Source: SGD
  • cellular response to heat Source: SGD
  • cellular response to hydrogen peroxide Source: SGD
  • coflocculation Source: SGD
  • flocculation Source: SGD
  • flocculation via cell wall protein-carbohydrate interaction Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-28884-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Flocculation protein FLO1
Short name:
Flocculin-1
Gene namesi
Name:FLO1
Synonyms:FLO2, FLO4, FLO8
Ordered Locus Names:YAR050W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:YAR050W.
SGDiS000000084. FLO1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell wall, Membrane, Secreted

Pathology & Biotechi

Biotechnological usei

For many industrial applications in which the yeast Saccharomyces cerevisiae is used, e.g. beer, wine and alcohol production, appropriate flocculation behavior is one of the most important characteristics of a good production strain. The ability of yeast cells to flocculate is of considerable importance, as it provides an effective, environment-friendly, simple and cost-free way to separate yeast cells from the fermentation product at the end of fermentation.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000002127325 – 1514Flocculation protein FLO1Add BLAST1490
PropeptideiPRO_00000212741515 – 1537Removed in mature formSequence analysisAdd BLAST23

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi135N-linked (GlcNAc...)Sequence analysis1
Glycosylationi187N-linked (GlcNAc...)Sequence analysis1
Glycosylationi262N-linked (GlcNAc...)Sequence analysis1
Glycosylationi329N-linked (GlcNAc...)Sequence analysis1
Glycosylationi374N-linked (GlcNAc...)Sequence analysis1
Glycosylationi419N-linked (GlcNAc...)Sequence analysis1
Glycosylationi464N-linked (GlcNAc...)Sequence analysis1
Glycosylationi509N-linked (GlcNAc...)Sequence analysis1
Glycosylationi554N-linked (GlcNAc...)Sequence analysis1
Glycosylationi599N-linked (GlcNAc...)Sequence analysis1
Glycosylationi644N-linked (GlcNAc...)Sequence analysis1
Glycosylationi689N-linked (GlcNAc...)Sequence analysis1
Glycosylationi734N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1114N-linked (GlcNAc...)Sequence analysis1
Lipidationi1514GPI-anchor amidated glycineSequence analysis1

Post-translational modificationi

Extensively N- and O-glycosylated.Curated
The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PRIDEiP32768.

Interactioni

Protein-protein interaction databases

BioGridi31813. 15 interactors.

Structurei

Secondary structure

11537
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi35 – 44Combined sources10
Helixi53 – 55Combined sources3
Helixi57 – 61Combined sources5
Helixi63 – 66Combined sources4
Beta strandi69 – 76Combined sources8
Beta strandi81 – 83Combined sources3
Beta strandi86 – 89Combined sources4
Beta strandi92 – 95Combined sources4
Helixi98 – 100Combined sources3
Turni103 – 105Combined sources3
Turni108 – 110Combined sources3
Turni125 – 128Combined sources4
Beta strandi135 – 144Combined sources10
Beta strandi147 – 157Combined sources11
Beta strandi160 – 168Combined sources9
Turni169 – 171Combined sources3
Beta strandi189 – 192Combined sources4
Beta strandi204 – 210Combined sources7
Beta strandi215 – 224Combined sources10
Beta strandi229 – 231Combined sources3
Beta strandi233 – 236Combined sources4
Beta strandi242 – 246Combined sources5
Turni248 – 250Combined sources3
Beta strandi251 – 254Combined sources4
Beta strandi264 – 266Combined sources3
Helixi268 – 270Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LHLX-ray1.43A23-271[»]
4LHNX-ray2.12A23-271[»]
ProteinModelPortaliP32768.
SMRiP32768.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati278 – 3221-1Add BLAST45
Repeati323 – 3671-2Add BLAST45
Repeati368 – 4121-3Add BLAST45
Repeati413 – 4571-4Add BLAST45
Repeati458 – 5021-5Add BLAST45
Repeati503 – 5471-6Add BLAST45
Repeati548 – 5921-7Add BLAST45
Repeati593 – 6371-8Add BLAST45
Repeati638 – 6821-9Add BLAST45
Repeati683 – 7271-10Add BLAST45
Repeati728 – 7721-11Add BLAST45
Repeati773 – 8171-12Add BLAST45
Repeati818 – 8621-13Add BLAST45
Repeati863 – 9071-14Add BLAST45
Repeati908 – 9521-15Add BLAST45
Repeati953 – 9971-16Add BLAST45
Repeati998 – 10421-17Add BLAST45
Repeati1043 – 10871-18Add BLAST45
Repeati1118 – 11372-1Add BLAST20
Repeati1138 – 11572-2Add BLAST20
Repeati1226 – 12763-1Add BLAST51
Repeati1291 – 13413-2Add BLAST51
Repeati1342 – 13923-3Add BLAST51
Repeati1408 – 14164-19
Repeati1417 – 14254-29
Repeati1426 – 14344-39

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni197 – 240Sugar recognitionAdd BLAST44
Regioni278 – 108718 X 45 AA approximate tandem repeats, Thr-richAdd BLAST810
Regioni1118 – 11572 X 20 AA approximate tandem repeats, Ser/Thr-richAdd BLAST40
Regioni1226 – 13923 X 51 AA approximate repeats, Ser/Thr-richAdd BLAST167
Regioni1408 – 14343 X 9 AA approximate tandem repeats, Thr-richAdd BLAST27

Domaini

The number of the intragenic tandem repeats varies between different S.cerevisiae strains. There is a linear correlation between protein size and the extend of adhesion: the more repeats, the stronger the adhesion properties and the greater the fraction of flocculating cells. The Ser/Thr-rich repeats are also important for proper cell wall targeting of the protein.

Sequence similaritiesi

Belongs to the flocculin family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

GeneTreeiENSGT00660000095872.
InParanoidiP32768.
OMAiYATNDNT.
OrthoDBiEOG092C2UYM.

Family and domain databases

Gene3Di3.90.182.10. 1 hit.
InterProiIPR001389. Flocculin.
IPR011658. PA14_dom.
[Graphical view]
PfamiPF00624. Flocculin. 18 hits.
PF07691. PA14. 1 hit.
[Graphical view]
SMARTiSM00758. PA14. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32768-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMPHRYMFL AVFTLLALTS VASGATEACL PAGQRKSGMN INFYQYSLKD
60 70 80 90 100
SSTYSNAAYM AYGYASKTKL GSVGGQTDIS IDYNIPCVSS SGTFPCPQED
110 120 130 140 150
SYGNWGCKGM GACSNSQGIA YWSTDLFGFY TTPTNVTLEM TGYFLPPQTG
160 170 180 190 200
SYTFKFATVD DSAILSVGGA TAFNCCAQQQ PPITSTNFTI DGIKPWGGSL
210 220 230 240 250
PPNIEGTVYM YAGYYYPMKV VYSNAVSWGT LPISVTLPDG TTVSDDFEGY
260 270 280 290 300
VYSFDDDLSQ SNCTVPDPSN YAVSTTTTTT EPWTGTFTST STEMTTVTGT
310 320 330 340 350
NGVPTDETVI VIRTPTTAST IITTTEPWNS TFTSTSTELT TVTGTNGVRT
360 370 380 390 400
DETIIVIRTP TTATTAITTT EPWNSTFTST STELTTVTGT NGLPTDETII
410 420 430 440 450
VIRTPTTATT AMTTTQPWND TFTSTSTELT TVTGTNGLPT DETIIVIRTP
460 470 480 490 500
TTATTAMTTT QPWNDTFTST STELTTVTGT NGLPTDETII VIRTPTTATT
510 520 530 540 550
AMTTTQPWND TFTSTSTEIT TVTGTNGLPT DETIIVIRTP TTATTAMTTP
560 570 580 590 600
QPWNDTFTST STEMTTVTGT NGLPTDETII VIRTPTTATT AITTTEPWNS
610 620 630 640 650
TFTSTSTEMT TVTGTNGLPT DETIIVIRTP TTATTAITTT QPWNDTFTST
660 670 680 690 700
STEMTTVTGT NGLPTDETII VIRTPTTATT AMTTTQPWND TFTSTSTEIT
710 720 730 740 750
TVTGTTGLPT DETIIVIRTP TTATTAMTTT QPWNDTFTST STEMTTVTGT
760 770 780 790 800
NGVPTDETVI VIRTPTSEGL ISTTTEPWTG TFTSTSTEMT TVTGTNGQPT
810 820 830 840 850
DETVIVIRTP TSEGLVTTTT EPWTGTFTST STEMTTITGT NGVPTDETVI
860 870 880 890 900
VIRTPTSEGL ISTTTEPWTG TFTSTSTEMT TITGTNGQPT DETVIVIRTP
910 920 930 940 950
TSEGLISTTT EPWTGTFTST STEMTHVTGT NGVPTDETVI VIRTPTSEGL
960 970 980 990 1000
ISTTTEPWTG TFTSTSTEVT TITGTNGQPT DETVIVIRTP TSEGLISTTT
1010 1020 1030 1040 1050
EPWTGTFTST STEMTTVTGT NGQPTDETVI VIRTPTSEGL VTTTTEPWTG
1060 1070 1080 1090 1100
TFTSTSTEMS TVTGTNGLPT DETVIVVKTP TTAISSSLSS SSSGQITSSI
1110 1120 1130 1140 1150
TSSRPIITPF YPSNGTSVIS SSVISSSVTS SLFTSSPVIS SSVISSSTTT
1160 1170 1180 1190 1200
STSIFSESSK SSVIPTSSST SGSSESETSS AGSVSSSSFI SSESSKSPTY
1210 1220 1230 1240 1250
SSSSLPLVTS ATTSQETASS LPPATTTKTS EQTTLVTVTS CESHVCTESI
1260 1270 1280 1290 1300
SPAIVSTATV TVSGVTTEYT TWCPISTTET TKQTKGTTEQ TTETTKQTTV
1310 1320 1330 1340 1350
VTISSCESDV CSKTASPAIV STSTATINGV TTEYTTWCPI STTESRQQTT
1360 1370 1380 1390 1400
LVTVTSCESG VCSETASPAI VSTATATVND VVTVYPTWRP QTANEESVSS
1410 1420 1430 1440 1450
KMNSATGETT TNTLAAETTT NTVAAETITN TGAAETKTVV TSSLSRSNHA
1460 1470 1480 1490 1500
ETQTASATDV IGHSSSVVSV SETGNTKSLT SSGLSTMSQQ PRSTPASSMV
1510 1520 1530
GYSTASLEIS TYAGSANSLL AGSGLSVFIA SLLLAII
Length:1,537
Mass (Da):160,668
Last modified:December 12, 2006 - v4
Checksum:iC7D4213C46ED23EF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti330S → G in AAX47297 (PubMed:16086015).Curated1
Sequence conflicti349R → P in AAX47297 (PubMed:16086015).Curated1
Sequence conflicti375S → G in AAX47297 (PubMed:16086015).Curated1
Sequence conflicti384L → M in AAX47297 (PubMed:16086015).Curated1
Sequence conflicti416 – 422QPWNDTF → HHGTTLL in AAX47297 (PubMed:16086015).Curated7
Sequence conflicti429L → M in CAA55024 (PubMed:7483845).Curated1
Sequence conflicti429L → M in AAX47297 (PubMed:16086015).Curated1
Sequence conflicti436N → K in AAX47297 (PubMed:16086015).Curated1
Sequence conflicti464N → D in CAA55024 (PubMed:7483845).Curated1
Sequence conflicti469S → P in AAX47297 (PubMed:16086015).Curated1
Sequence conflicti474L → M in CAA55024 (PubMed:7483845).Curated1
Sequence conflicti519I → M in CAA55024 (PubMed:7483845).Curated1
Sequence conflicti550P → T in CAA55024 (PubMed:7483845).Curated1
Sequence conflicti609M → L in CAA55024 (PubMed:7483845).Curated1
Sequence conflicti637I → M in CAA55024 (PubMed:7483845).Curated1
Sequence conflicti699I → M in CAA55024 (PubMed:7483845).Curated1
Sequence conflicti706T → N in CAA55024 (PubMed:7483845).Curated1
Sequence conflicti926H → T in CAA55024 (PubMed:7483845).Curated1
Sequence conflicti926H → T in AAX47294 (PubMed:16086015).Curated1
Sequence conflicti926H → T in AAX47295 (PubMed:16086015).Curated1
Sequence conflicti926H → T in AAX47297 (PubMed:16086015).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti303 – 797Missing in strain: S288c / KV295. Add BLAST495
Natural varianti317 – 946Missing in strain: S288c / KV333. Add BLAST630
Natural varianti317 – 901Missing in strain: S288c / KV291. Add BLAST585

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78160 Genomic DNA. Translation: CAA55024.1.
AY949845 Genomic DNA. Translation: AAX47294.1.
AY949846 Genomic DNA. Translation: AAX47295.1.
AY949847 Genomic DNA. Translation: AAX47296.1.
AY949848 Genomic DNA. Translation: AAX47297.1.
EF670005 Genomic DNA. Translation: ABS87371.1.
L28920 Genomic DNA. Translation: AAC09499.1. Sequence problems.
BK006935 Genomic DNA. Translation: DAA07007.1.
PIRiS53465.
RefSeqiNP_009424.1. NM_001178230.1.

Genome annotation databases

EnsemblFungiiYAR050W; YAR050W; YAR050W.
GeneIDi851289.
KEGGisce:YAR050W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78160 Genomic DNA. Translation: CAA55024.1.
AY949845 Genomic DNA. Translation: AAX47294.1.
AY949846 Genomic DNA. Translation: AAX47295.1.
AY949847 Genomic DNA. Translation: AAX47296.1.
AY949848 Genomic DNA. Translation: AAX47297.1.
EF670005 Genomic DNA. Translation: ABS87371.1.
L28920 Genomic DNA. Translation: AAC09499.1. Sequence problems.
BK006935 Genomic DNA. Translation: DAA07007.1.
PIRiS53465.
RefSeqiNP_009424.1. NM_001178230.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LHLX-ray1.43A23-271[»]
4LHNX-ray2.12A23-271[»]
ProteinModelPortaliP32768.
SMRiP32768.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31813. 15 interactors.

Proteomic databases

PRIDEiP32768.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYAR050W; YAR050W; YAR050W.
GeneIDi851289.
KEGGisce:YAR050W.

Organism-specific databases

EuPathDBiFungiDB:YAR050W.
SGDiS000000084. FLO1.

Phylogenomic databases

GeneTreeiENSGT00660000095872.
InParanoidiP32768.
OMAiYATNDNT.
OrthoDBiEOG092C2UYM.

Enzyme and pathway databases

BioCyciYEAST:G3O-28884-MONOMER.

Miscellaneous databases

PROiP32768.

Family and domain databases

Gene3Di3.90.182.10. 1 hit.
InterProiIPR001389. Flocculin.
IPR011658. PA14_dom.
[Graphical view]
PfamiPF00624. Flocculin. 18 hits.
PF07691. PA14. 1 hit.
[Graphical view]
SMARTiSM00758. PA14. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFLO1_YEAST
AccessioniPrimary (citable) accession number: P32768
Secondary accession number(s): A7U4Y7
, D6VPN7, Q58HH7, Q58HH8, Q58HH9, Q58HI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: December 12, 2006
Last modified: November 2, 2016
This is version 130 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.