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P32768

- FLO1_YEAST

UniProt

P32768 - FLO1_YEAST

Protein

Flocculation protein FLO1

Gene

FLO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 4 (12 Dec 2006)
      Previous versions | rss
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    Functioni

    Cell wall protein that participates directly in adhesive cell-cell interactions during yeast flocculation, a reversible, asexual and Ca2+-dependent process in which cells adhere to form aggregates (flocs) consisting of thousands of cells. The lectin-like protein sticks out of the cell wall of flocculent cells and selectively binds mannose residues in the cell walls of adjacent cells. Activity is inhibited by mannose, but not by glucose, maltose, sucrose or galactose. Also involved in cell-substrate adhesion.4 Publications

    GO - Molecular functioni

    1. mannose binding Source: SGD

    GO - Biological processi

    1. cellular response to ethanol Source: SGD
    2. cellular response to heat Source: SGD
    3. cellular response to hydrogen peroxide Source: SGD
    4. flocculation Source: SGD
    5. flocculation via cell wall protein-carbohydrate interaction Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28884-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Flocculation protein FLO1
    Short name:
    Flocculin-1
    Gene namesi
    Name:FLO1
    Synonyms:FLO2, FLO4, FLO8
    Ordered Locus Names:YAR050W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome I

    Organism-specific databases

    CYGDiYAR050w.
    SGDiS000000084. FLO1.

    Subcellular locationi

    Cell membrane; Lipid-anchorGPI-anchor. Secretedcell wall
    Note: Identified as GPI-anchored plasma membrane protein (GPI-PMP) as well as covalently-linked GPI-modified cell wall protein (GPI-CWP) in the outer cell wall layer.

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. extracellular region Source: UniProtKB-KW
    3. fungal-type cell wall Source: SGD
    4. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cell wall, Membrane, Secreted

    Pathology & Biotechi

    Biotechnological usei

    For many industrial applications in which the yeast Saccharomyces cerevisiae is used, e.g. beer, wine and alcohol production, appropriate flocculation behavior is one of the most important characteristics of a good production strain. The ability of yeast cells to flocculate is of considerable importance, as it provides an effective, environment-friendly, simple and cost-free way to separate yeast cells from the fermentation product at the end of fermentation.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 15141490Flocculation protein FLO1PRO_0000021273Add
    BLAST
    Propeptidei1515 – 153723Removed in mature formSequence AnalysisPRO_0000021274Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi135 – 1351N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi187 – 1871N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi262 – 2621N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi329 – 3291N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi374 – 3741N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi419 – 4191N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi464 – 4641N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi509 – 5091N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi554 – 5541N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi599 – 5991N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi644 – 6441N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi689 – 6891N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi734 – 7341N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1114 – 11141N-linked (GlcNAc...)Sequence Analysis
    Lipidationi1514 – 15141GPI-anchor amidated glycineSequence Analysis

    Post-translational modificationi

    Extensively N- and O-glycosylated.Curated
    The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

    Keywords - PTMi

    Glycoprotein, GPI-anchor, Lipoprotein

    Expressioni

    Gene expression databases

    GenevestigatoriP32768.

    Interactioni

    Protein-protein interaction databases

    BioGridi31813. 15 interactions.
    STRINGi4932.YAR050W.

    Structurei

    3D structure databases

    ProteinModelPortaliP32768.
    SMRiP32768. Positions 23-269.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati278 – 322451-1Add
    BLAST
    Repeati323 – 367451-2Add
    BLAST
    Repeati368 – 412451-3Add
    BLAST
    Repeati413 – 457451-4Add
    BLAST
    Repeati458 – 502451-5Add
    BLAST
    Repeati503 – 547451-6Add
    BLAST
    Repeati548 – 592451-7Add
    BLAST
    Repeati593 – 637451-8Add
    BLAST
    Repeati638 – 682451-9Add
    BLAST
    Repeati683 – 727451-10Add
    BLAST
    Repeati728 – 772451-11Add
    BLAST
    Repeati773 – 817451-12Add
    BLAST
    Repeati818 – 862451-13Add
    BLAST
    Repeati863 – 907451-14Add
    BLAST
    Repeati908 – 952451-15Add
    BLAST
    Repeati953 – 997451-16Add
    BLAST
    Repeati998 – 1042451-17Add
    BLAST
    Repeati1043 – 1087451-18Add
    BLAST
    Repeati1118 – 1137202-1Add
    BLAST
    Repeati1138 – 1157202-2Add
    BLAST
    Repeati1226 – 1276513-1Add
    BLAST
    Repeati1291 – 1341513-2Add
    BLAST
    Repeati1342 – 1392513-3Add
    BLAST
    Repeati1408 – 141694-1
    Repeati1417 – 142594-2
    Repeati1426 – 143494-3

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni197 – 24044Sugar recognitionAdd
    BLAST
    Regioni278 – 108781018 X 45 AA approximate tandem repeats, Thr-richAdd
    BLAST
    Regioni1118 – 1157402 X 20 AA approximate tandem repeats, Ser/Thr-richAdd
    BLAST
    Regioni1226 – 13921673 X 51 AA approximate repeats, Ser/Thr-richAdd
    BLAST
    Regioni1408 – 1434273 X 9 AA approximate tandem repeats, Thr-richAdd
    BLAST

    Domaini

    The number of the intragenic tandem repeats varies between different S.cerevisiae strains. There is a linear correlation between protein size and the extend of adhesion: the more repeats, the stronger the adhesion properties and the greater the fraction of flocculating cells. The Ser/Thr-rich repeats are also important for proper cell wall targeting of the protein.

    Sequence similaritiesi

    Belongs to the flocculin family.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    GeneTreeiENSGT00660000095872.
    OMAiCDIHKDG.
    OrthoDBiEOG7H4F6F.

    Family and domain databases

    Gene3Di3.90.182.10. 1 hit.
    InterProiIPR001389. Flocculin.
    IPR011658. PA14.
    [Graphical view]
    PfamiPF00624. Flocculin. 18 hits.
    PF07691. PA14. 1 hit.
    [Graphical view]
    SMARTiSM00758. PA14. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P32768-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTMPHRYMFL AVFTLLALTS VASGATEACL PAGQRKSGMN INFYQYSLKD     50
    SSTYSNAAYM AYGYASKTKL GSVGGQTDIS IDYNIPCVSS SGTFPCPQED 100
    SYGNWGCKGM GACSNSQGIA YWSTDLFGFY TTPTNVTLEM TGYFLPPQTG 150
    SYTFKFATVD DSAILSVGGA TAFNCCAQQQ PPITSTNFTI DGIKPWGGSL 200
    PPNIEGTVYM YAGYYYPMKV VYSNAVSWGT LPISVTLPDG TTVSDDFEGY 250
    VYSFDDDLSQ SNCTVPDPSN YAVSTTTTTT EPWTGTFTST STEMTTVTGT 300
    NGVPTDETVI VIRTPTTAST IITTTEPWNS TFTSTSTELT TVTGTNGVRT 350
    DETIIVIRTP TTATTAITTT EPWNSTFTST STELTTVTGT NGLPTDETII 400
    VIRTPTTATT AMTTTQPWND TFTSTSTELT TVTGTNGLPT DETIIVIRTP 450
    TTATTAMTTT QPWNDTFTST STELTTVTGT NGLPTDETII VIRTPTTATT 500
    AMTTTQPWND TFTSTSTEIT TVTGTNGLPT DETIIVIRTP TTATTAMTTP 550
    QPWNDTFTST STEMTTVTGT NGLPTDETII VIRTPTTATT AITTTEPWNS 600
    TFTSTSTEMT TVTGTNGLPT DETIIVIRTP TTATTAITTT QPWNDTFTST 650
    STEMTTVTGT NGLPTDETII VIRTPTTATT AMTTTQPWND TFTSTSTEIT 700
    TVTGTTGLPT DETIIVIRTP TTATTAMTTT QPWNDTFTST STEMTTVTGT 750
    NGVPTDETVI VIRTPTSEGL ISTTTEPWTG TFTSTSTEMT TVTGTNGQPT 800
    DETVIVIRTP TSEGLVTTTT EPWTGTFTST STEMTTITGT NGVPTDETVI 850
    VIRTPTSEGL ISTTTEPWTG TFTSTSTEMT TITGTNGQPT DETVIVIRTP 900
    TSEGLISTTT EPWTGTFTST STEMTHVTGT NGVPTDETVI VIRTPTSEGL 950
    ISTTTEPWTG TFTSTSTEVT TITGTNGQPT DETVIVIRTP TSEGLISTTT 1000
    EPWTGTFTST STEMTTVTGT NGQPTDETVI VIRTPTSEGL VTTTTEPWTG 1050
    TFTSTSTEMS TVTGTNGLPT DETVIVVKTP TTAISSSLSS SSSGQITSSI 1100
    TSSRPIITPF YPSNGTSVIS SSVISSSVTS SLFTSSPVIS SSVISSSTTT 1150
    STSIFSESSK SSVIPTSSST SGSSESETSS AGSVSSSSFI SSESSKSPTY 1200
    SSSSLPLVTS ATTSQETASS LPPATTTKTS EQTTLVTVTS CESHVCTESI 1250
    SPAIVSTATV TVSGVTTEYT TWCPISTTET TKQTKGTTEQ TTETTKQTTV 1300
    VTISSCESDV CSKTASPAIV STSTATINGV TTEYTTWCPI STTESRQQTT 1350
    LVTVTSCESG VCSETASPAI VSTATATVND VVTVYPTWRP QTANEESVSS 1400
    KMNSATGETT TNTLAAETTT NTVAAETITN TGAAETKTVV TSSLSRSNHA 1450
    ETQTASATDV IGHSSSVVSV SETGNTKSLT SSGLSTMSQQ PRSTPASSMV 1500
    GYSTASLEIS TYAGSANSLL AGSGLSVFIA SLLLAII 1537
    Length:1,537
    Mass (Da):160,668
    Last modified:December 12, 2006 - v4
    Checksum:iC7D4213C46ED23EF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti330 – 3301S → G in AAX47297. (PubMed:16086015)Curated
    Sequence conflicti349 – 3491R → P in AAX47297. (PubMed:16086015)Curated
    Sequence conflicti375 – 3751S → G in AAX47297. (PubMed:16086015)Curated
    Sequence conflicti384 – 3841L → M in AAX47297. (PubMed:16086015)Curated
    Sequence conflicti416 – 4227QPWNDTF → HHGTTLL in AAX47297. (PubMed:16086015)Curated
    Sequence conflicti429 – 4291L → M in CAA55024. (PubMed:7483845)Curated
    Sequence conflicti429 – 4291L → M in AAX47297. (PubMed:16086015)Curated
    Sequence conflicti436 – 4361N → K in AAX47297. (PubMed:16086015)Curated
    Sequence conflicti464 – 4641N → D in CAA55024. (PubMed:7483845)Curated
    Sequence conflicti469 – 4691S → P in AAX47297. (PubMed:16086015)Curated
    Sequence conflicti474 – 4741L → M in CAA55024. (PubMed:7483845)Curated
    Sequence conflicti519 – 5191I → M in CAA55024. (PubMed:7483845)Curated
    Sequence conflicti550 – 5501P → T in CAA55024. (PubMed:7483845)Curated
    Sequence conflicti609 – 6091M → L in CAA55024. (PubMed:7483845)Curated
    Sequence conflicti637 – 6371I → M in CAA55024. (PubMed:7483845)Curated
    Sequence conflicti699 – 6991I → M in CAA55024. (PubMed:7483845)Curated
    Sequence conflicti706 – 7061T → N in CAA55024. (PubMed:7483845)Curated
    Sequence conflicti926 – 9261H → T in CAA55024. (PubMed:7483845)Curated
    Sequence conflicti926 – 9261H → T in AAX47294. (PubMed:16086015)Curated
    Sequence conflicti926 – 9261H → T in AAX47295. (PubMed:16086015)Curated
    Sequence conflicti926 – 9261H → T in AAX47297. (PubMed:16086015)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti303 – 797495Missing in strain: S288c / KV295.
    Add
    BLAST
    Natural varianti317 – 946630Missing in strain: S288c / KV333.
    Add
    BLAST
    Natural varianti317 – 901585Missing in strain: S288c / KV291.
    Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78160 Genomic DNA. Translation: CAA55024.1.
    AY949845 Genomic DNA. Translation: AAX47294.1.
    AY949846 Genomic DNA. Translation: AAX47295.1.
    AY949847 Genomic DNA. Translation: AAX47296.1.
    AY949848 Genomic DNA. Translation: AAX47297.1.
    EF670005 Genomic DNA. Translation: ABS87371.1.
    L28920 Genomic DNA. Translation: AAC09499.1. Sequence problems.
    BK006935 Genomic DNA. Translation: DAA07007.1.
    PIRiS53465.
    RefSeqiNP_009424.1. NM_001178230.1.

    Genome annotation databases

    EnsemblFungiiYAR050W; YAR050W; YAR050W.
    GeneIDi851289.
    KEGGisce:YAR050W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78160 Genomic DNA. Translation: CAA55024.1 .
    AY949845 Genomic DNA. Translation: AAX47294.1 .
    AY949846 Genomic DNA. Translation: AAX47295.1 .
    AY949847 Genomic DNA. Translation: AAX47296.1 .
    AY949848 Genomic DNA. Translation: AAX47297.1 .
    EF670005 Genomic DNA. Translation: ABS87371.1 .
    L28920 Genomic DNA. Translation: AAC09499.1 . Sequence problems.
    BK006935 Genomic DNA. Translation: DAA07007.1 .
    PIRi S53465.
    RefSeqi NP_009424.1. NM_001178230.1.

    3D structure databases

    ProteinModelPortali P32768.
    SMRi P32768. Positions 23-269.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31813. 15 interactions.
    STRINGi 4932.YAR050W.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YAR050W ; YAR050W ; YAR050W .
    GeneIDi 851289.
    KEGGi sce:YAR050W.

    Organism-specific databases

    CYGDi YAR050w.
    SGDi S000000084. FLO1.

    Phylogenomic databases

    GeneTreei ENSGT00660000095872.
    OMAi CDIHKDG.
    OrthoDBi EOG7H4F6F.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28884-MONOMER.

    Miscellaneous databases

    NextBioi 968291.

    Gene expression databases

    Genevestigatori P32768.

    Family and domain databases

    Gene3Di 3.90.182.10. 1 hit.
    InterProi IPR001389. Flocculin.
    IPR011658. PA14.
    [Graphical view ]
    Pfami PF00624. Flocculin. 18 hits.
    PF07691. PA14. 1 hit.
    [Graphical view ]
    SMARTi SM00758. PA14. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of the open reading frame of the FLO1 gene from Saccharomyces cerevisiae."
      Teunissen A.W.R.H., Holub E., van der Hucht J., van den Berg J.A., Steensma H.Y.
      Yeast 9:423-427(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The Saccharomyces cerevisiae FLO1 flocculation gene encodes for a cell surface protein."
      Bidard F., Bony M., Blondin B., Dequin S., Barre P.
      Yeast 11:809-822(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, REPEATS.
      Strain: STX347-1D.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Intragenic tandem repeats generate functional variability."
      Verstrepen K.J., Jansen A., Lewitter F., Fink G.R.
      Nat. Genet. 37:986-990(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], REPEATS.
      Strain: S288c / KV1, S288c / KV291, S288c / KV295 and S288c / KV333.
    5. "Differential Flo8p-dependent regulation of FLO1 and FLO11 for cell-cell and cell-substrate adherence of S.cerevisiae S288c."
      Fichtner L., Schulze F., Braus G.H.
      Mol. Microbiol. 66:1276-1289(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
      Strain: Sigma 1278B.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    7. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    8. "Localization of the dominant flocculation genes FLO5 and FLO8 of Saccharomyces cerevisiae."
      Teunissen A.W.R.H., van den Berg J.A., Steensma H.Y.
      Yeast 11:735-745(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE MAPPING.
    9. "Review: the dominant flocculation genes of Saccharomyces cerevisiae constitute a new subtelomeric gene family."
      Teunissen A.W.R.H., Steensma H.Y.
      Yeast 11:1001-1013(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    10. "The retention mechanism of cell wall proteins in Saccharomyces cerevisiae. Wall-bound Cwp2p is beta-1,6-glucosylated."
      van der Vaart J.M., van Schagen F.S., Mooren A.T.A., Chapman J.W., Klis F.M., Verrips C.T.
      Biochim. Biophys. Acta 1291:206-214(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    11. "Localization and cell surface anchoring of the Saccharomyces cerevisiae flocculation protein Flo1p."
      Bony M., Thines-Sempoux D., Barre P., Blondin B.
      J. Bacteriol. 179:4929-4936(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    12. "Region of FLO1 proteins responsible for sugar recognition."
      Kobayashi O., Hayashi N., Kuroki R., Sone H.
      J. Bacteriol. 180:6503-6510(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    13. "Distribution of the flocculation protein, flop, at the cell surface during yeast growth: the availability of flop determines the flocculation level."
      Bony M., Barre P., Blondin B.
      Yeast 14:25-35(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    14. "A Saccharomyces gene family involved in invasive growth, cell-cell adhesion, and mating."
      Guo B., Styles C.A., Feng Q., Fink G.R.
      Proc. Natl. Acad. Sci. U.S.A. 97:12158-12163(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    15. Cited for: BIOTECHNOLOGY.
    16. Cited for: BIOTECHNOLOGY.
    17. "Multiple sequence signals determine the distribution of glycosylphosphatidylinositol proteins between the plasma membrane and cell wall in Saccharomyces cerevisiae."
      Frieman M.B., Cormack B.P.
      Microbiology 150:3105-3114(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, REPEATS.

    Entry informationi

    Entry nameiFLO1_YEAST
    AccessioniPrimary (citable) accession number: P32768
    Secondary accession number(s): A7U4Y7
    , D6VPN7, Q58HH7, Q58HH8, Q58HH9, Q58HI0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: December 12, 2006
    Last modified: October 1, 2014
    This is version 113 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome I
      Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

    External Data

    Dasty 3