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P32768

- FLO1_YEAST

UniProt

P32768 - FLO1_YEAST

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Protein

Flocculation protein FLO1

Gene
FLO1, FLO2, FLO4, FLO8, YAR050W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Cell wall protein that participates directly in adhesive cell-cell interactions during yeast flocculation, a reversible, asexual and Ca2+-dependent process in which cells adhere to form aggregates (flocs) consisting of thousands of cells. The lectin-like protein sticks out of the cell wall of flocculent cells and selectively binds mannose residues in the cell walls of adjacent cells. Activity is inhibited by mannose, but not by glucose, maltose, sucrose or galactose. Also involved in cell-substrate adhesion.4 Publications

GO - Molecular functioni

  1. mannose binding Source: SGD

GO - Biological processi

  1. cellular response to ethanol Source: SGD
  2. cellular response to heat Source: SGD
  3. cellular response to hydrogen peroxide Source: SGD
  4. flocculation Source: SGD
  5. flocculation via cell wall protein-carbohydrate interaction Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-28884-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Flocculation protein FLO1
Short name:
Flocculin-1
Gene namesi
Name:FLO1
Synonyms:FLO2, FLO4, FLO8
Ordered Locus Names:YAR050W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome I

Organism-specific databases

CYGDiYAR050w.
SGDiS000000084. FLO1.

Subcellular locationi

Cell membrane; Lipid-anchorGPI-anchor. Secretedcell wall
Note: Identified as GPI-anchored plasma membrane protein (GPI-PMP) as well as covalently-linked GPI-modified cell wall protein (GPI-CWP) in the outer cell wall layer.5 Publications

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. extracellular region Source: UniProtKB-KW
  3. fungal-type cell wall Source: SGD
  4. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell wall, Membrane, Secreted

Pathology & Biotechi

Biotechnological usei

For many industrial applications in which the yeast Saccharomyces cerevisiae is used, e.g. beer, wine and alcohol production, appropriate flocculation behavior is one of the most important characteristics of a good production strain. The ability of yeast cells to flocculate is of considerable importance, as it provides an effective, environment-friendly, simple and cost-free way to separate yeast cells from the fermentation product at the end of fermentation.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424 Reviewed predictionAdd
BLAST
Chaini25 – 15141490Flocculation protein FLO1PRO_0000021273Add
BLAST
Propeptidei1515 – 153723Removed in mature form Reviewed predictionPRO_0000021274Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi135 – 1351N-linked (GlcNAc...) Reviewed prediction
Glycosylationi187 – 1871N-linked (GlcNAc...) Reviewed prediction
Glycosylationi262 – 2621N-linked (GlcNAc...) Reviewed prediction
Glycosylationi329 – 3291N-linked (GlcNAc...) Reviewed prediction
Glycosylationi374 – 3741N-linked (GlcNAc...) Reviewed prediction
Glycosylationi419 – 4191N-linked (GlcNAc...) Reviewed prediction
Glycosylationi464 – 4641N-linked (GlcNAc...) Reviewed prediction
Glycosylationi509 – 5091N-linked (GlcNAc...) Reviewed prediction
Glycosylationi554 – 5541N-linked (GlcNAc...) Reviewed prediction
Glycosylationi599 – 5991N-linked (GlcNAc...) Reviewed prediction
Glycosylationi644 – 6441N-linked (GlcNAc...) Reviewed prediction
Glycosylationi689 – 6891N-linked (GlcNAc...) Reviewed prediction
Glycosylationi734 – 7341N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1114 – 11141N-linked (GlcNAc...) Reviewed prediction
Lipidationi1514 – 15141GPI-anchor amidated glycine Reviewed prediction

Post-translational modificationi

Extensively N- and O-glycosylated Inferred.
The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Expressioni

Gene expression databases

GenevestigatoriP32768.

Interactioni

Protein-protein interaction databases

BioGridi31813. 15 interactions.
STRINGi4932.YAR050W.

Structurei

3D structure databases

ProteinModelPortaliP32768.
SMRiP32768. Positions 23-269.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati278 – 322451-1Add
BLAST
Repeati323 – 367451-2Add
BLAST
Repeati368 – 412451-3Add
BLAST
Repeati413 – 457451-4Add
BLAST
Repeati458 – 502451-5Add
BLAST
Repeati503 – 547451-6Add
BLAST
Repeati548 – 592451-7Add
BLAST
Repeati593 – 637451-8Add
BLAST
Repeati638 – 682451-9Add
BLAST
Repeati683 – 727451-10Add
BLAST
Repeati728 – 772451-11Add
BLAST
Repeati773 – 817451-12Add
BLAST
Repeati818 – 862451-13Add
BLAST
Repeati863 – 907451-14Add
BLAST
Repeati908 – 952451-15Add
BLAST
Repeati953 – 997451-16Add
BLAST
Repeati998 – 1042451-17Add
BLAST
Repeati1043 – 1087451-18Add
BLAST
Repeati1118 – 1137202-1Add
BLAST
Repeati1138 – 1157202-2Add
BLAST
Repeati1226 – 1276513-1Add
BLAST
Repeati1291 – 1341513-2Add
BLAST
Repeati1342 – 1392513-3Add
BLAST
Repeati1408 – 141694-1
Repeati1417 – 142594-2
Repeati1426 – 143494-3

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni197 – 24044Sugar recognitionAdd
BLAST
Regioni278 – 108781018 X 45 AA approximate tandem repeats, Thr-richAdd
BLAST
Regioni1118 – 1157402 X 20 AA approximate tandem repeats, Ser/Thr-richAdd
BLAST
Regioni1226 – 13921673 X 51 AA approximate repeats, Ser/Thr-richAdd
BLAST
Regioni1408 – 1434273 X 9 AA approximate tandem repeats, Thr-richAdd
BLAST

Domaini

The number of the intragenic tandem repeats varies between different S.cerevisiae strains. There is a linear correlation between protein size and the extend of adhesion: the more repeats, the stronger the adhesion properties and the greater the fraction of flocculating cells. The Ser/Thr-rich repeats are also important for proper cell wall targeting of the protein.

Sequence similaritiesi

Belongs to the flocculin family.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

GeneTreeiENSGT00660000095872.
OMAiCDIHKDG.
OrthoDBiEOG7H4F6F.

Family and domain databases

Gene3Di3.90.182.10. 1 hit.
InterProiIPR001389. Flocculin.
IPR011658. PA14.
[Graphical view]
PfamiPF00624. Flocculin. 18 hits.
PF07691. PA14. 1 hit.
[Graphical view]
SMARTiSM00758. PA14. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32768-1 [UniParc]FASTAAdd to Basket

« Hide

MTMPHRYMFL AVFTLLALTS VASGATEACL PAGQRKSGMN INFYQYSLKD     50
SSTYSNAAYM AYGYASKTKL GSVGGQTDIS IDYNIPCVSS SGTFPCPQED 100
SYGNWGCKGM GACSNSQGIA YWSTDLFGFY TTPTNVTLEM TGYFLPPQTG 150
SYTFKFATVD DSAILSVGGA TAFNCCAQQQ PPITSTNFTI DGIKPWGGSL 200
PPNIEGTVYM YAGYYYPMKV VYSNAVSWGT LPISVTLPDG TTVSDDFEGY 250
VYSFDDDLSQ SNCTVPDPSN YAVSTTTTTT EPWTGTFTST STEMTTVTGT 300
NGVPTDETVI VIRTPTTAST IITTTEPWNS TFTSTSTELT TVTGTNGVRT 350
DETIIVIRTP TTATTAITTT EPWNSTFTST STELTTVTGT NGLPTDETII 400
VIRTPTTATT AMTTTQPWND TFTSTSTELT TVTGTNGLPT DETIIVIRTP 450
TTATTAMTTT QPWNDTFTST STELTTVTGT NGLPTDETII VIRTPTTATT 500
AMTTTQPWND TFTSTSTEIT TVTGTNGLPT DETIIVIRTP TTATTAMTTP 550
QPWNDTFTST STEMTTVTGT NGLPTDETII VIRTPTTATT AITTTEPWNS 600
TFTSTSTEMT TVTGTNGLPT DETIIVIRTP TTATTAITTT QPWNDTFTST 650
STEMTTVTGT NGLPTDETII VIRTPTTATT AMTTTQPWND TFTSTSTEIT 700
TVTGTTGLPT DETIIVIRTP TTATTAMTTT QPWNDTFTST STEMTTVTGT 750
NGVPTDETVI VIRTPTSEGL ISTTTEPWTG TFTSTSTEMT TVTGTNGQPT 800
DETVIVIRTP TSEGLVTTTT EPWTGTFTST STEMTTITGT NGVPTDETVI 850
VIRTPTSEGL ISTTTEPWTG TFTSTSTEMT TITGTNGQPT DETVIVIRTP 900
TSEGLISTTT EPWTGTFTST STEMTHVTGT NGVPTDETVI VIRTPTSEGL 950
ISTTTEPWTG TFTSTSTEVT TITGTNGQPT DETVIVIRTP TSEGLISTTT 1000
EPWTGTFTST STEMTTVTGT NGQPTDETVI VIRTPTSEGL VTTTTEPWTG 1050
TFTSTSTEMS TVTGTNGLPT DETVIVVKTP TTAISSSLSS SSSGQITSSI 1100
TSSRPIITPF YPSNGTSVIS SSVISSSVTS SLFTSSPVIS SSVISSSTTT 1150
STSIFSESSK SSVIPTSSST SGSSESETSS AGSVSSSSFI SSESSKSPTY 1200
SSSSLPLVTS ATTSQETASS LPPATTTKTS EQTTLVTVTS CESHVCTESI 1250
SPAIVSTATV TVSGVTTEYT TWCPISTTET TKQTKGTTEQ TTETTKQTTV 1300
VTISSCESDV CSKTASPAIV STSTATINGV TTEYTTWCPI STTESRQQTT 1350
LVTVTSCESG VCSETASPAI VSTATATVND VVTVYPTWRP QTANEESVSS 1400
KMNSATGETT TNTLAAETTT NTVAAETITN TGAAETKTVV TSSLSRSNHA 1450
ETQTASATDV IGHSSSVVSV SETGNTKSLT SSGLSTMSQQ PRSTPASSMV 1500
GYSTASLEIS TYAGSANSLL AGSGLSVFIA SLLLAII 1537
Length:1,537
Mass (Da):160,668
Last modified:December 12, 2006 - v4
Checksum:iC7D4213C46ED23EF
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti303 – 797495Missing in strain: S288c / KV295.
Add
BLAST
Natural varianti317 – 946630Missing in strain: S288c / KV333.
Add
BLAST
Natural varianti317 – 901585Missing in strain: S288c / KV291.
Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti330 – 3301S → G in AAX47297. 1 Publication
Sequence conflicti349 – 3491R → P in AAX47297. 1 Publication
Sequence conflicti375 – 3751S → G in AAX47297. 1 Publication
Sequence conflicti384 – 3841L → M in AAX47297. 1 Publication
Sequence conflicti416 – 4227QPWNDTF → HHGTTLL in AAX47297. 1 Publication
Sequence conflicti429 – 4291L → M in CAA55024. 1 Publication
Sequence conflicti429 – 4291L → M in AAX47297. 1 Publication
Sequence conflicti436 – 4361N → K in AAX47297. 1 Publication
Sequence conflicti464 – 4641N → D in CAA55024. 1 Publication
Sequence conflicti469 – 4691S → P in AAX47297. 1 Publication
Sequence conflicti474 – 4741L → M in CAA55024. 1 Publication
Sequence conflicti519 – 5191I → M in CAA55024. 1 Publication
Sequence conflicti550 – 5501P → T in CAA55024. 1 Publication
Sequence conflicti609 – 6091M → L in CAA55024. 1 Publication
Sequence conflicti637 – 6371I → M in CAA55024. 1 Publication
Sequence conflicti699 – 6991I → M in CAA55024. 1 Publication
Sequence conflicti706 – 7061T → N in CAA55024. 1 Publication
Sequence conflicti926 – 9261H → T in CAA55024. 1 Publication
Sequence conflicti926 – 9261H → T in AAX47294. 1 Publication
Sequence conflicti926 – 9261H → T in AAX47295. 1 Publication
Sequence conflicti926 – 9261H → T in AAX47297. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78160 Genomic DNA. Translation: CAA55024.1.
AY949845 Genomic DNA. Translation: AAX47294.1.
AY949846 Genomic DNA. Translation: AAX47295.1.
AY949847 Genomic DNA. Translation: AAX47296.1.
AY949848 Genomic DNA. Translation: AAX47297.1.
EF670005 Genomic DNA. Translation: ABS87371.1.
L28920 Genomic DNA. Translation: AAC09499.1. Sequence problems.
BK006935 Genomic DNA. Translation: DAA07007.1.
PIRiS53465.
RefSeqiNP_009424.1. NM_001178230.1.

Genome annotation databases

EnsemblFungiiYAR050W; YAR050W; YAR050W.
GeneIDi851289.
KEGGisce:YAR050W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78160 Genomic DNA. Translation: CAA55024.1 .
AY949845 Genomic DNA. Translation: AAX47294.1 .
AY949846 Genomic DNA. Translation: AAX47295.1 .
AY949847 Genomic DNA. Translation: AAX47296.1 .
AY949848 Genomic DNA. Translation: AAX47297.1 .
EF670005 Genomic DNA. Translation: ABS87371.1 .
L28920 Genomic DNA. Translation: AAC09499.1 . Sequence problems.
BK006935 Genomic DNA. Translation: DAA07007.1 .
PIRi S53465.
RefSeqi NP_009424.1. NM_001178230.1.

3D structure databases

ProteinModelPortali P32768.
SMRi P32768. Positions 23-269.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31813. 15 interactions.
STRINGi 4932.YAR050W.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YAR050W ; YAR050W ; YAR050W .
GeneIDi 851289.
KEGGi sce:YAR050W.

Organism-specific databases

CYGDi YAR050w.
SGDi S000000084. FLO1.

Phylogenomic databases

GeneTreei ENSGT00660000095872.
OMAi CDIHKDG.
OrthoDBi EOG7H4F6F.

Enzyme and pathway databases

BioCyci YEAST:G3O-28884-MONOMER.

Miscellaneous databases

NextBioi 968291.

Gene expression databases

Genevestigatori P32768.

Family and domain databases

Gene3Di 3.90.182.10. 1 hit.
InterProi IPR001389. Flocculin.
IPR011658. PA14.
[Graphical view ]
Pfami PF00624. Flocculin. 18 hits.
PF07691. PA14. 1 hit.
[Graphical view ]
SMARTi SM00758. PA14. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of the open reading frame of the FLO1 gene from Saccharomyces cerevisiae."
    Teunissen A.W.R.H., Holub E., van der Hucht J., van den Berg J.A., Steensma H.Y.
    Yeast 9:423-427(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The Saccharomyces cerevisiae FLO1 flocculation gene encodes for a cell surface protein."
    Bidard F., Bony M., Blondin B., Dequin S., Barre P.
    Yeast 11:809-822(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, REPEATS.
    Strain: STX347-1D.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Intragenic tandem repeats generate functional variability."
    Verstrepen K.J., Jansen A., Lewitter F., Fink G.R.
    Nat. Genet. 37:986-990(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], REPEATS.
    Strain: S288c / KV1, S288c / KV291, S288c / KV295 and S288c / KV333.
  5. "Differential Flo8p-dependent regulation of FLO1 and FLO11 for cell-cell and cell-substrate adherence of S.cerevisiae S288c."
    Fichtner L., Schulze F., Braus G.H.
    Mol. Microbiol. 66:1276-1289(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: Sigma 1278B.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  7. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  8. "Localization of the dominant flocculation genes FLO5 and FLO8 of Saccharomyces cerevisiae."
    Teunissen A.W.R.H., van den Berg J.A., Steensma H.Y.
    Yeast 11:735-745(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE MAPPING.
  9. "Review: the dominant flocculation genes of Saccharomyces cerevisiae constitute a new subtelomeric gene family."
    Teunissen A.W.R.H., Steensma H.Y.
    Yeast 11:1001-1013(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  10. "The retention mechanism of cell wall proteins in Saccharomyces cerevisiae. Wall-bound Cwp2p is beta-1,6-glucosylated."
    van der Vaart J.M., van Schagen F.S., Mooren A.T.A., Chapman J.W., Klis F.M., Verrips C.T.
    Biochim. Biophys. Acta 1291:206-214(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  11. "Localization and cell surface anchoring of the Saccharomyces cerevisiae flocculation protein Flo1p."
    Bony M., Thines-Sempoux D., Barre P., Blondin B.
    J. Bacteriol. 179:4929-4936(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  12. "Region of FLO1 proteins responsible for sugar recognition."
    Kobayashi O., Hayashi N., Kuroki R., Sone H.
    J. Bacteriol. 180:6503-6510(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "Distribution of the flocculation protein, flop, at the cell surface during yeast growth: the availability of flop determines the flocculation level."
    Bony M., Barre P., Blondin B.
    Yeast 14:25-35(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  14. "A Saccharomyces gene family involved in invasive growth, cell-cell adhesion, and mating."
    Guo B., Styles C.A., Feng Q., Fink G.R.
    Proc. Natl. Acad. Sci. U.S.A. 97:12158-12163(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  15. Cited for: BIOTECHNOLOGY.
  16. Cited for: BIOTECHNOLOGY.
  17. "Multiple sequence signals determine the distribution of glycosylphosphatidylinositol proteins between the plasma membrane and cell wall in Saccharomyces cerevisiae."
    Frieman M.B., Cormack B.P.
    Microbiology 150:3105-3114(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, REPEATS.

Entry informationi

Entry nameiFLO1_YEAST
AccessioniPrimary (citable) accession number: P32768
Secondary accession number(s): A7U4Y7
, D6VPN7, Q58HH7, Q58HH8, Q58HH9, Q58HI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: December 12, 2006
Last modified: May 14, 2014
This is version 112 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

External Data

Dasty 3

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