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P32768 (FLO1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Flocculation protein FLO1

Short name=Flocculin-1
Gene names
Name:FLO1
Synonyms:FLO2, FLO4, FLO8
Ordered Locus Names:YAR050W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1537 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell wall protein that participates directly in adhesive cell-cell interactions during yeast flocculation, a reversible, asexual and Ca2+-dependent process in which cells adhere to form aggregates (flocs) consisting of thousands of cells. The lectin-like protein sticks out of the cell wall of flocculent cells and selectively binds mannose residues in the cell walls of adjacent cells. Activity is inhibited by mannose, but not by glucose, maltose, sucrose or galactose. Also involved in cell-substrate adhesion. Ref.5 Ref.12 Ref.13 Ref.14

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor. Secretedcell wall. Note: Identified as GPI-anchored plasma membrane protein (GPI-PMP) as well as covalently-linked GPI-modified cell wall protein (GPI-CWP) in the outer cell wall layer. Ref.2 Ref.10 Ref.11 Ref.13 Ref.17

Domain

The number of the intragenic tandem repeats varies between different S.cerevisiae strains. There is a linear correlation between protein size and the extend of adhesion: the more repeats, the stronger the adhesion properties and the greater the fraction of flocculating cells. The Ser/Thr-rich repeats are also important for proper cell wall targeting of the protein.

Post-translational modification

Extensively N- and O-glycosylated Probable.

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

Biotechnological use

For many industrial applications in which the yeast Saccharomyces cerevisiae is used, e.g. beer, wine and alcohol production, appropriate flocculation behavior is one of the most important characteristics of a good production strain. The ability of yeast cells to flocculate is of considerable importance, as it provides an effective, environment-friendly, simple and cost-free way to separate yeast cells from the fermentation product at the end of fermentation. Ref.15 Ref.16

Sequence similarities

Belongs to the flocculin family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 15141490Flocculation protein FLO1
PRO_0000021273
Propeptide1515 – 153723Removed in mature form Potential
PRO_0000021274

Regions

Repeat278 – 322451-1
Repeat323 – 367451-2
Repeat368 – 412451-3
Repeat413 – 457451-4
Repeat458 – 502451-5
Repeat503 – 547451-6
Repeat548 – 592451-7
Repeat593 – 637451-8
Repeat638 – 682451-9
Repeat683 – 727451-10
Repeat728 – 772451-11
Repeat773 – 817451-12
Repeat818 – 862451-13
Repeat863 – 907451-14
Repeat908 – 952451-15
Repeat953 – 997451-16
Repeat998 – 1042451-17
Repeat1043 – 1087451-18
Repeat1118 – 1137202-1
Repeat1138 – 1157202-2
Repeat1226 – 1276513-1
Repeat1291 – 1341513-2
Repeat1342 – 1392513-3
Repeat1408 – 141694-1
Repeat1417 – 142594-2
Repeat1426 – 143494-3
Region197 – 24044Sugar recognition
Region278 – 108781018 X 45 AA approximate tandem repeats, Thr-rich
Region1118 – 1157402 X 20 AA approximate tandem repeats, Ser/Thr-rich
Region1226 – 13921673 X 51 AA approximate repeats, Ser/Thr-rich
Region1408 – 1434273 X 9 AA approximate tandem repeats, Thr-rich

Amino acid modifications

Lipidation15141GPI-anchor amidated glycine Potential
Glycosylation1351N-linked (GlcNAc...) Potential
Glycosylation1871N-linked (GlcNAc...) Potential
Glycosylation2621N-linked (GlcNAc...) Potential
Glycosylation3291N-linked (GlcNAc...) Potential
Glycosylation3741N-linked (GlcNAc...) Potential
Glycosylation4191N-linked (GlcNAc...) Potential
Glycosylation4641N-linked (GlcNAc...) Potential
Glycosylation5091N-linked (GlcNAc...) Potential
Glycosylation5541N-linked (GlcNAc...) Potential
Glycosylation5991N-linked (GlcNAc...) Potential
Glycosylation6441N-linked (GlcNAc...) Potential
Glycosylation6891N-linked (GlcNAc...) Potential
Glycosylation7341N-linked (GlcNAc...) Potential
Glycosylation11141N-linked (GlcNAc...) Potential

Natural variations

Natural variant303 – 797495Missing in strain: S288c / KV295.
Natural variant317 – 946630Missing in strain: S288c / KV333.
Natural variant317 – 901585Missing in strain: S288c / KV291.

Experimental info

Sequence conflict3301S → G in AAX47297. Ref.4
Sequence conflict3491R → P in AAX47297. Ref.4
Sequence conflict3751S → G in AAX47297. Ref.4
Sequence conflict3841L → M in AAX47297. Ref.4
Sequence conflict416 – 4227QPWNDTF → HHGTTLL in AAX47297. Ref.4
Sequence conflict4291L → M in CAA55024. Ref.2
Sequence conflict4291L → M in AAX47297. Ref.4
Sequence conflict4361N → K in AAX47297. Ref.4
Sequence conflict4641N → D in CAA55024. Ref.2
Sequence conflict4691S → P in AAX47297. Ref.4
Sequence conflict4741L → M in CAA55024. Ref.2
Sequence conflict5191I → M in CAA55024. Ref.2
Sequence conflict5501P → T in CAA55024. Ref.2
Sequence conflict6091M → L in CAA55024. Ref.2
Sequence conflict6371I → M in CAA55024. Ref.2
Sequence conflict6991I → M in CAA55024. Ref.2
Sequence conflict7061T → N in CAA55024. Ref.2
Sequence conflict9261H → T in CAA55024. Ref.2
Sequence conflict9261H → T in AAX47294. Ref.4
Sequence conflict9261H → T in AAX47295. Ref.4
Sequence conflict9261H → T in AAX47297. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P32768 [UniParc].

Last modified December 12, 2006. Version 4.
Checksum: C7D4213C46ED23EF

FASTA1,537160,668
        10         20         30         40         50         60 
MTMPHRYMFL AVFTLLALTS VASGATEACL PAGQRKSGMN INFYQYSLKD SSTYSNAAYM 

        70         80         90        100        110        120 
AYGYASKTKL GSVGGQTDIS IDYNIPCVSS SGTFPCPQED SYGNWGCKGM GACSNSQGIA 

       130        140        150        160        170        180 
YWSTDLFGFY TTPTNVTLEM TGYFLPPQTG SYTFKFATVD DSAILSVGGA TAFNCCAQQQ 

       190        200        210        220        230        240 
PPITSTNFTI DGIKPWGGSL PPNIEGTVYM YAGYYYPMKV VYSNAVSWGT LPISVTLPDG 

       250        260        270        280        290        300 
TTVSDDFEGY VYSFDDDLSQ SNCTVPDPSN YAVSTTTTTT EPWTGTFTST STEMTTVTGT 

       310        320        330        340        350        360 
NGVPTDETVI VIRTPTTAST IITTTEPWNS TFTSTSTELT TVTGTNGVRT DETIIVIRTP 

       370        380        390        400        410        420 
TTATTAITTT EPWNSTFTST STELTTVTGT NGLPTDETII VIRTPTTATT AMTTTQPWND 

       430        440        450        460        470        480 
TFTSTSTELT TVTGTNGLPT DETIIVIRTP TTATTAMTTT QPWNDTFTST STELTTVTGT 

       490        500        510        520        530        540 
NGLPTDETII VIRTPTTATT AMTTTQPWND TFTSTSTEIT TVTGTNGLPT DETIIVIRTP 

       550        560        570        580        590        600 
TTATTAMTTP QPWNDTFTST STEMTTVTGT NGLPTDETII VIRTPTTATT AITTTEPWNS 

       610        620        630        640        650        660 
TFTSTSTEMT TVTGTNGLPT DETIIVIRTP TTATTAITTT QPWNDTFTST STEMTTVTGT 

       670        680        690        700        710        720 
NGLPTDETII VIRTPTTATT AMTTTQPWND TFTSTSTEIT TVTGTTGLPT DETIIVIRTP 

       730        740        750        760        770        780 
TTATTAMTTT QPWNDTFTST STEMTTVTGT NGVPTDETVI VIRTPTSEGL ISTTTEPWTG 

       790        800        810        820        830        840 
TFTSTSTEMT TVTGTNGQPT DETVIVIRTP TSEGLVTTTT EPWTGTFTST STEMTTITGT 

       850        860        870        880        890        900 
NGVPTDETVI VIRTPTSEGL ISTTTEPWTG TFTSTSTEMT TITGTNGQPT DETVIVIRTP 

       910        920        930        940        950        960 
TSEGLISTTT EPWTGTFTST STEMTHVTGT NGVPTDETVI VIRTPTSEGL ISTTTEPWTG 

       970        980        990       1000       1010       1020 
TFTSTSTEVT TITGTNGQPT DETVIVIRTP TSEGLISTTT EPWTGTFTST STEMTTVTGT 

      1030       1040       1050       1060       1070       1080 
NGQPTDETVI VIRTPTSEGL VTTTTEPWTG TFTSTSTEMS TVTGTNGLPT DETVIVVKTP 

      1090       1100       1110       1120       1130       1140 
TTAISSSLSS SSSGQITSSI TSSRPIITPF YPSNGTSVIS SSVISSSVTS SLFTSSPVIS 

      1150       1160       1170       1180       1190       1200 
SSVISSSTTT STSIFSESSK SSVIPTSSST SGSSESETSS AGSVSSSSFI SSESSKSPTY 

      1210       1220       1230       1240       1250       1260 
SSSSLPLVTS ATTSQETASS LPPATTTKTS EQTTLVTVTS CESHVCTESI SPAIVSTATV 

      1270       1280       1290       1300       1310       1320 
TVSGVTTEYT TWCPISTTET TKQTKGTTEQ TTETTKQTTV VTISSCESDV CSKTASPAIV 

      1330       1340       1350       1360       1370       1380 
STSTATINGV TTEYTTWCPI STTESRQQTT LVTVTSCESG VCSETASPAI VSTATATVND 

      1390       1400       1410       1420       1430       1440 
VVTVYPTWRP QTANEESVSS KMNSATGETT TNTLAAETTT NTVAAETITN TGAAETKTVV 

      1450       1460       1470       1480       1490       1500 
TSSLSRSNHA ETQTASATDV IGHSSSVVSV SETGNTKSLT SSGLSTMSQQ PRSTPASSMV 

      1510       1520       1530 
GYSTASLEIS TYAGSANSLL AGSGLSVFIA SLLLAII 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of the open reading frame of the FLO1 gene from Saccharomyces cerevisiae."
Teunissen A.W.R.H., Holub E., van der Hucht J., van den Berg J.A., Steensma H.Y.
Yeast 9:423-427(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The Saccharomyces cerevisiae FLO1 flocculation gene encodes for a cell surface protein."
Bidard F., Bony M., Blondin B., Dequin S., Barre P.
Yeast 11:809-822(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, REPEATS.
Strain: STX347-1D.
[3]"Molecular cloning and analysis of the yeast flocculation gene FLO1."
Watari J., Takata Y., Ogawa M., Sahara H., Koshino S., Onnela M.-L., Airaksinen U., Jaatinen R., Penttilae M., Keraenen S.
Yeast 10:211-225(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Intragenic tandem repeats generate functional variability."
Verstrepen K.J., Jansen A., Lewitter F., Fink G.R.
Nat. Genet. 37:986-990(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], REPEATS.
Strain: S288c / KV1, S288c / KV291, S288c / KV295 and S288c / KV333.
[5]"Differential Flo8p-dependent regulation of FLO1 and FLO11 for cell-cell and cell-substrate adherence of S.cerevisiae S288c."
Fichtner L., Schulze F., Braus G.H.
Mol. Microbiol. 66:1276-1289(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
Strain: Sigma 1278B.
[6]"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae."
Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K.
Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[7]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[8]"Localization of the dominant flocculation genes FLO5 and FLO8 of Saccharomyces cerevisiae."
Teunissen A.W.R.H., van den Berg J.A., Steensma H.Y.
Yeast 11:735-745(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE MAPPING.
[9]"Review: the dominant flocculation genes of Saccharomyces cerevisiae constitute a new subtelomeric gene family."
Teunissen A.W.R.H., Steensma H.Y.
Yeast 11:1001-1013(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[10]"The retention mechanism of cell wall proteins in Saccharomyces cerevisiae. Wall-bound Cwp2p is beta-1,6-glucosylated."
van der Vaart J.M., van Schagen F.S., Mooren A.T.A., Chapman J.W., Klis F.M., Verrips C.T.
Biochim. Biophys. Acta 1291:206-214(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[11]"Localization and cell surface anchoring of the Saccharomyces cerevisiae flocculation protein Flo1p."
Bony M., Thines-Sempoux D., Barre P., Blondin B.
J. Bacteriol. 179:4929-4936(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[12]"Region of FLO1 proteins responsible for sugar recognition."
Kobayashi O., Hayashi N., Kuroki R., Sone H.
J. Bacteriol. 180:6503-6510(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[13]"Distribution of the flocculation protein, flop, at the cell surface during yeast growth: the availability of flop determines the flocculation level."
Bony M., Barre P., Blondin B.
Yeast 14:25-35(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[14]"A Saccharomyces gene family involved in invasive growth, cell-cell adhesion, and mating."
Guo B., Styles C.A., Feng Q., Fink G.R.
Proc. Natl. Acad. Sci. U.S.A. 97:12158-12163(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[15]"Late fermentation expression of FLO1 in Saccharomyces cerevisiae."
Verstrepen K.J., Michiels C., Derdelinckx G., Delvaux F.R., Winderickx J., Thevelein J.M., Bauer F.F., Pretorius I.S.
J. Am. Soc. Brew. Chem. 59:69-76(2001)
Cited for: BIOTECHNOLOGY.
[16]"Yeast flocculation: what brewers should know."
Verstrepen K.J., Derdelinckx G., Verachtert H., Delvaux F.R.
Appl. Microbiol. Biotechnol. 61:197-205(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: BIOTECHNOLOGY.
[17]"Multiple sequence signals determine the distribution of glycosylphosphatidylinositol proteins between the plasma membrane and cell wall in Saccharomyces cerevisiae."
Frieman M.B., Cormack B.P.
Microbiology 150:3105-3114(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, REPEATS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X78160 Genomic DNA. Translation: CAA55024.1.
AY949845 Genomic DNA. Translation: AAX47294.1.
AY949846 Genomic DNA. Translation: AAX47295.1.
AY949847 Genomic DNA. Translation: AAX47296.1.
AY949848 Genomic DNA. Translation: AAX47297.1.
EF670005 Genomic DNA. Translation: ABS87371.1.
L28920 Genomic DNA. Translation: AAC09499.1. Sequence problems.
BK006935 Genomic DNA. Translation: DAA07007.1.
PIRS53465.
RefSeqNP_009424.1. NM_001178230.1.

3D structure databases

ProteinModelPortalP32768.
SMRP32768. Positions 23-269.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31813. 15 interactions.
STRING4932.YAR050W.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYAR050W; YAR050W; YAR050W.
GeneID851289.
KEGGsce:YAR050W.

Organism-specific databases

CYGDYAR050w.
SGDS000000084. FLO1.

Phylogenomic databases

GeneTreeENSGT00660000095872.
OMAKTLYAFA.
OrthoDBEOG7H4F6F.

Enzyme and pathway databases

BioCycYEAST:G3O-28884-MONOMER.

Gene expression databases

GenevestigatorP32768.

Family and domain databases

Gene3D3.90.182.10. 1 hit.
InterProIPR001389. Flocculin.
IPR011658. PA14.
[Graphical view]
PfamPF00624. Flocculin. 18 hits.
PF07691. PA14. 1 hit.
[Graphical view]
SMARTSM00758. PA14. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio968291.

Entry information

Entry nameFLO1_YEAST
AccessionPrimary (citable) accession number: P32768
Secondary accession number(s): A7U4Y7 expand/collapse secondary AC list , D6VPN7, Q58HH7, Q58HH8, Q58HH9, Q58HI0
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: December 12, 2006
Last modified: April 16, 2014
This is version 111 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome I

Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families