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P32767

- KA122_YEAST

UniProt

P32767 - KA122_YEAST

Protein

Importin beta-like protein KAP122

Gene

KAP122

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm. Required for the nuclear import of the complex composed the large subunit (TOA1) and the small subunit (TOA2) of the general transcription factor IIA (TFIIA). Required for the nuclear import of the RNR2-RNR4 heterodimer, also called beta-beta' subunit, which corresponds to the small subunit of the ribonucleotide reductase (RNR). May play a role in regulation of pleiotropic drug resistance.4 Publications

    GO - Molecular functioni

    1. protein transporter activity Source: SGD

    GO - Biological processi

    1. protein import into nucleus Source: SGD

    Keywords - Biological processi

    Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30537-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Importin beta-like protein KAP122
    Alternative name(s):
    Karyopherin-122
    Pleiotropic drug resistance regulatory protein 6
    Gene namesi
    Name:KAP122
    Synonyms:PDR6
    Ordered Locus Names:YGL016W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL016w.
    SGDiS000002984. KAP122.

    Subcellular locationi

    Nucleus envelope 2 Publications

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. nuclear envelope Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10811081Importin beta-like protein KAP122PRO_0000058276Add
    BLAST

    Proteomic databases

    MaxQBiP32767.
    PaxDbiP32767.
    PeptideAtlasiP32767.

    Expressioni

    Gene expression databases

    GenevestigatoriP32767.

    Interactioni

    Subunit structurei

    Associates with the nuclear pore complex (NPC). Interacts with GSP2, NUP1, NUP2, TOA1, TOA2 and WTM1.3 Publications

    Protein-protein interaction databases

    BioGridi33230. 89 interactions.
    DIPiDIP-2449N.
    IntActiP32767. 13 interactions.
    MINTiMINT-707770.
    STRINGi4932.YGL016W.

    Structurei

    3D structure databases

    ProteinModelPortaliP32767.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the importin beta family.Curated

    Phylogenomic databases

    eggNOGiNOG260621.
    OMAiHLYISTM.
    OrthoDBiEOG7XSTP0.

    Family and domain databases

    InterProiIPR016024. ARM-type_fold.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 3 hits.

    Sequencei

    Sequence statusi: Complete.

    P32767-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSIHEVVAL IEELYSPHPK HDVNQIQQSL QSIQKSEQGF HLANELLSDD     50
    KYSANVKYFG ALTLTVQLNT RGENDYETLW NVFRSNLLYL TKFSTLYVSN 100
    PNMYGQSLII IKKLMSNLSL IFTKINDPQL NNAGNENMIK QWNNPINTFI 150
    QLMSVQNQNI NADQLLLDSI NCSLTYEQLS QFVSLSQKHN ELALTFTEVI 200
    VEDLTKFQTK RHSMSQIHEV VHEHLYISTM ALINLNLTAQ AVFNPTVFDC 250
    ITAWINYISL TRSVSSSGRM DLSEIFQNLI DLMYQSTEGS DGYENAEKIL 300
    TIFGNVFAND PLLMSYDLRQ QIECIFLGVV RPDSGITDIS NKNSWMLQYM 350
    NYLVTNDFFS ELKELAICIV DFLQINTLSV CNKLFTNIQA ADNGQVQDEY 400
    IQEYIKVLLQ MTNFPLTPVL QEFFSVRMVD FWLDLSDAYT NLASETLRPN 450
    SIELSTQIFQ QLINIYLPKI SLSVKQRIIE EEGESTSVNE FEDFRNAVSD 500
    LAQSLWSILG NDNLTNVLID GMGQMPAASD ETLIIKDTDV LFRIETMCFV 550
    LNTILVDMTL SESPWIKNIV DANKFFNQNV ISVFQTGFQT SASTKVSQIL 600
    KLDFVRTSTT LIGTLAGYFK QEPFQLNPYV EALFQGLHTC TNFTSKNEQE 650
    KISNDKLEVM VIKTVSTLCE TCREELTPYL MHFISFLNTV IMPDSNVSHF 700
    TRTKLVRSIG YVVQCQVSNG PEEQAKYILQ LTNLLSGSIE HCLASSVQLQ 750
    EQQDYINCLL YCISELATSL IQPTEIIEND ALLQRLSEFQ SFWSSDPLQI 800
    RSKIMCTIDK VLDNSIYCKN SAFVEIGCLI VGKGLNLPDG EPYFLKYNMS 850
    EVMNFVLRHV PNCELATCLP YFVYLLEKLI SEFRKELTPQ EFDFMFEKIL 900
    LVYYDAYIIN DPDLLQMTIG FVNNVLDVKP GLAIGSKHWT SFILPQFLKL 950
    IPSREKFTIV AVAKFWTKLI NNKKYNQEEL TTVRQQVSSI GGDLVYQIMY 1000
    GLFHTQRSDL NSYTDLLRAL VAKFPIEARE WLVAVLPQIC NNPAGHEKFI 1050
    NKLLITRGSR AAGNVILQWW LDCTTLPNYQ G 1081
    Length:1,081
    Mass (Da):123,531
    Last modified:October 1, 1993 - v1
    Checksum:iEEBC5ADD4E16D9D3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S58126 Genomic DNA. Translation: AAD13901.1.
    S57895 Genomic DNA. Translation: AAB19613.1.
    Z72538 Genomic DNA. Translation: CAA96716.1.
    BK006941 Genomic DNA. Translation: DAA08082.1.
    PIRiS15040.
    RefSeqiNP_011499.1. NM_001180881.1.

    Genome annotation databases

    EnsemblFungiiYGL016W; YGL016W; YGL016W.
    GeneIDi852868.
    KEGGisce:YGL016W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S58126 Genomic DNA. Translation: AAD13901.1 .
    S57895 Genomic DNA. Translation: AAB19613.1 .
    Z72538 Genomic DNA. Translation: CAA96716.1 .
    BK006941 Genomic DNA. Translation: DAA08082.1 .
    PIRi S15040.
    RefSeqi NP_011499.1. NM_001180881.1.

    3D structure databases

    ProteinModelPortali P32767.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33230. 89 interactions.
    DIPi DIP-2449N.
    IntActi P32767. 13 interactions.
    MINTi MINT-707770.
    STRINGi 4932.YGL016W.

    Proteomic databases

    MaxQBi P32767.
    PaxDbi P32767.
    PeptideAtlasi P32767.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL016W ; YGL016W ; YGL016W .
    GeneIDi 852868.
    KEGGi sce:YGL016W.

    Organism-specific databases

    CYGDi YGL016w.
    SGDi S000002984. KAP122.

    Phylogenomic databases

    eggNOGi NOG260621.
    OMAi HLYISTM.
    OrthoDBi EOG7XSTP0.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30537-MONOMER.

    Miscellaneous databases

    NextBioi 972490.

    Gene expression databases

    Genevestigatori P32767.

    Family and domain databases

    InterProi IPR016024. ARM-type_fold.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 3 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The DNA sequencing of the 17 kb HindIII fragment spanning the LEU1 and ATE1 loci on chromosome VII from Saccharomyces cerevisiae reveals the PDR6 gene, a new member of the genetic network controlling pleiotropic drug resistance."
      Chen W., Balzi E., Capieaux E., Choder M., Goffeau A.
      Yeast 7:287-299(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
      Strain: ATCC 46191 / IL125-2B.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The karyopherin Kap122p/Pdr6p imports both subunits of the transcription factor IIA into the nucleus."
      Titov A.A., Blobel G.
      J. Cell Biol. 147:235-246(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH NUP1; NUP2; TOA1 AND TOA2, FUNCTION.
    5. "Purification of protein A-tagged yeast ran reveals association with a novel karyopherin beta family member, Pdr6p."
      Lau D., Kunzler M., Braunwarth A., Hellmuth K., Podtelejnikov A., Mann M., Hurt E.
      J. Biol. Chem. 275:467-471(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH GSP2.
    6. "The yeast nuclear pore complex: composition, architecture, and transport mechanism."
      Rout M.P., Aitchison J.D., Suprapto A., Hjertaas K., Zhao Y., Chait B.T.
      J. Cell Biol. 148:635-651(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, ASSOCIATION WITH THE NUCLEAR PORE COMPLEX.
    7. "Nuclear localization of the Saccharomyces cerevisiae ribonucleotide reductase small subunit requires a karyopherin and a WD40 repeat protein."
      Zhang Z., An X., Yang K., Perlstein D.L., Hicks L., Kelleher N., Stubbe J., Huang M.
      Proc. Natl. Acad. Sci. U.S.A. 103:1422-1427(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH WTM1.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Dif1 controls subcellular localization of ribonucleotide reductase by mediating nuclear import of the R2 subunit."
      Wu X., Huang M.
      Mol. Cell. Biol. 28:7156-7167(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiKA122_YEAST
    AccessioniPrimary (citable) accession number: P32767
    Secondary accession number(s): D6VUC1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 9760 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3