P32767 (KA122_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Importin beta-like protein KAP122

Alternative name(s):
Karyopherin-122
Pleiotropic drug resistance regulatory protein 6

Gene names

Name:	KAP122
Synonyms:	PDR6
Ordered Locus Names:	YGL016W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1081 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm. Required for the nuclear import of the complex composed the large subunit (TOA1) and the small subunit (TOA2) of the general transcription factor IIA (TFIIA). Required for the nuclear import of the RNR2-RNR4 heterodimer, also called beta-beta' subunit, which corresponds to the small subunit of the ribonucleotide reductase (RNR). May play a role in regulation of pleiotropic drug resistance. Ref.1 Ref.4 Ref.7 Ref.9
Subunit structure	Associates with the nuclear pore complex (NPC). Interacts with GSP2, NUP1, NUP2, TOA1, TOA2 and WTM1. Ref.4 Ref.5 Ref.7
Subcellular location	Nucleus envelope Ref.4 Ref.5.
Miscellaneous	Present with 9760 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the importin beta family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Nucleus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein import into nucleus Inferred from direct assay Ref.4. Source: SGD regulation of cell size Inferred from mutant phenotype PubMed 12089449. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay Ref.6. Source: SGD nuclear pore Inferred from direct assay Ref.6. Source: SGD
Molecular_function	protein transporter activity Inferred from mutant phenotype Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1081	1081	Importin beta-like protein KAP122		PRO_0000058276

Sequences

	Sequence		Length	Mass (Da)
	P32767 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: EEBC5ADD4E16D9D3	FASTA	1,081	123,531
	10 20 30 40 50 60 MSSIHEVVAL IEELYSPHPK HDVNQIQQSL QSIQKSEQGF HLANELLSDD KYSANVKYFG 70 80 90 100 110 120 ALTLTVQLNT RGENDYETLW NVFRSNLLYL TKFSTLYVSN PNMYGQSLII IKKLMSNLSL 130 140 150 160 170 180 IFTKINDPQL NNAGNENMIK QWNNPINTFI QLMSVQNQNI NADQLLLDSI NCSLTYEQLS 190 200 210 220 230 240 QFVSLSQKHN ELALTFTEVI VEDLTKFQTK RHSMSQIHEV VHEHLYISTM ALINLNLTAQ 250 260 270 280 290 300 AVFNPTVFDC ITAWINYISL TRSVSSSGRM DLSEIFQNLI DLMYQSTEGS DGYENAEKIL 310 320 330 340 350 360 TIFGNVFAND PLLMSYDLRQ QIECIFLGVV RPDSGITDIS NKNSWMLQYM NYLVTNDFFS 370 380 390 400 410 420 ELKELAICIV DFLQINTLSV CNKLFTNIQA ADNGQVQDEY IQEYIKVLLQ MTNFPLTPVL 430 440 450 460 470 480 QEFFSVRMVD FWLDLSDAYT NLASETLRPN SIELSTQIFQ QLINIYLPKI SLSVKQRIIE 490 500 510 520 530 540 EEGESTSVNE FEDFRNAVSD LAQSLWSILG NDNLTNVLID GMGQMPAASD ETLIIKDTDV 550 560 570 580 590 600 LFRIETMCFV LNTILVDMTL SESPWIKNIV DANKFFNQNV ISVFQTGFQT SASTKVSQIL 610 620 630 640 650 660 KLDFVRTSTT LIGTLAGYFK QEPFQLNPYV EALFQGLHTC TNFTSKNEQE KISNDKLEVM 670 680 690 700 710 720 VIKTVSTLCE TCREELTPYL MHFISFLNTV IMPDSNVSHF TRTKLVRSIG YVVQCQVSNG 730 740 750 760 770 780 PEEQAKYILQ LTNLLSGSIE HCLASSVQLQ EQQDYINCLL YCISELATSL IQPTEIIEND 790 800 810 820 830 840 ALLQRLSEFQ SFWSSDPLQI RSKIMCTIDK VLDNSIYCKN SAFVEIGCLI VGKGLNLPDG 850 860 870 880 890 900 EPYFLKYNMS EVMNFVLRHV PNCELATCLP YFVYLLEKLI SEFRKELTPQ EFDFMFEKIL 910 920 930 940 950 960 LVYYDAYIIN DPDLLQMTIG FVNNVLDVKP GLAIGSKHWT SFILPQFLKL IPSREKFTIV 970 980 990 1000 1010 1020 AVAKFWTKLI NNKKYNQEEL TTVRQQVSSI GGDLVYQIMY GLFHTQRSDL NSYTDLLRAL 1030 1040 1050 1060 1070 1080 VAKFPIEARE WLVAVLPQIC NNPAGHEKFI NKLLITRGSR AAGNVILQWW LDCTTLPNYQ G

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The DNA sequencing of the 17 kb HindIII fragment spanning the LEU1 and ATE1 loci on chromosome VII from Saccharomyces cerevisiae reveals the PDR6 gene, a new member of the genetic network controlling pleiotropic drug resistance." Chen W., Balzi E., Capieaux E., Choder M., Goffeau A. Yeast 7:287-299(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: ATCC 46191 / IL125-2B.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The karyopherin Kap122p/Pdr6p imports both subunits of the transcription factor IIA into the nucleus." Titov A.A., Blobel G. J. Cell Biol. 147:235-246(1999) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH NUP1; NUP2; TOA1 AND TOA2, FUNCTION.
[5]	"Purification of protein A-tagged yeast ran reveals association with a novel karyopherin beta family member, Pdr6p." Lau D., Kunzler M., Braunwarth A., Hellmuth K., Podtelejnikov A., Mann M., Hurt E. J. Biol. Chem. 275:467-471(2000) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH GSP2.
[6]	"The yeast nuclear pore complex: composition, architecture, and transport mechanism." Rout M.P., Aitchison J.D., Suprapto A., Hjertaas K., Zhao Y., Chait B.T. J. Cell Biol. 148:635-651(2000) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, ASSOCIATION WITH THE NUCLEAR PORE COMPLEX.
[7]	"Nuclear localization of the Saccharomyces cerevisiae ribonucleotide reductase small subunit requires a karyopherin and a WD40 repeat protein." Zhang Z., An X., Yang K., Perlstein D.L., Hicks L., Kelleher N., Stubbe J., Huang M. Proc. Natl. Acad. Sci. U.S.A. 103:1422-1427(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH WTM1.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Dif1 controls subcellular localization of ribonucleotide reductase by mediating nuclear import of the R2 subunit." Wu X., Huang M. Mol. Cell. Biol. 28:7156-7167(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S58126 Genomic DNA. Translation: AAD13901.1. S57895 Genomic DNA. Translation: AAB19613.1. Z72538 Genomic DNA. Translation: CAA96716.1. BK006941 Genomic DNA. Translation: DAA08082.1.
PIR	S15040.
RefSeq	NP_011499.1. NM_001180881.1.
3D structure databases
ProteinModelPortal	P32767.
SMR	P32767. Positions 656-687.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2449N.
IntAct	P32767. 16 interactions.
MINT	MINT-707770.
STRING	4932.YGL016W.
Proteomic databases
PaxDb	P32767.
PeptideAtlas	P32767.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL016W; YGL016W; YGL016W.
GeneID	852868.
KEGG	sce:YGL016W.
Organism-specific databases
CYGD	YGL016w.
SGD	S000002984. KAP122.
Phylogenomic databases
eggNOG	NOG260621.
OMA	HEHLYIS.
OrthoDB	EOG405W89.
Gene expression databases
Genevestigator	P32767.
GermOnline	YGL016W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016024. ARM-type_fold. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
ProtoNet	Search...
Other
NextBio	972490.

Entry information

Entry name

KA122_YEAST

Accession

Primary (citable) accession number: P32767
Secondary accession number(s): D6VUC1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program