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P32767 (KA122_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Importin beta-like protein KAP122
Alternative name(s):
Karyopherin-122
Pleiotropic drug resistance regulatory protein 6
Gene names
Name:KAP122
Synonyms:PDR6
Ordered Locus Names:YGL016W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1081 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm. Required for the nuclear import of the complex composed the large subunit (TOA1) and the small subunit (TOA2) of the general transcription factor IIA (TFIIA). Required for the nuclear import of the RNR2-RNR4 heterodimer, also called beta-beta' subunit, which corresponds to the small subunit of the ribonucleotide reductase (RNR). May play a role in regulation of pleiotropic drug resistance. Ref.1 Ref.4 Ref.7 Ref.9

Subunit structure

Associates with the nuclear pore complex (NPC). Interacts with GSP2, NUP1, NUP2, TOA1, TOA2 and WTM1. Ref.4 Ref.5 Ref.7

Subcellular location

Nucleus envelope Ref.4 Ref.5.

Miscellaneous

Present with 9760 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the importin beta family.

Ontologies

Keywords
   Biological processTransport
   Cellular componentNucleus
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein import into nucleus

Inferred from direct assay Ref.4. Source: SGD

   Cellular_componentcytoplasm

Inferred from direct assay Ref.6. Source: SGD

nuclear envelope

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionprotein transporter activity

Inferred from mutant phenotype Ref.4. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10811081Importin beta-like protein KAP122
PRO_0000058276

Sequences

Sequence LengthMass (Da)Tools
P32767 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: EEBC5ADD4E16D9D3

FASTA1,081123,531
        10         20         30         40         50         60 
MSSIHEVVAL IEELYSPHPK HDVNQIQQSL QSIQKSEQGF HLANELLSDD KYSANVKYFG 

        70         80         90        100        110        120 
ALTLTVQLNT RGENDYETLW NVFRSNLLYL TKFSTLYVSN PNMYGQSLII IKKLMSNLSL 

       130        140        150        160        170        180 
IFTKINDPQL NNAGNENMIK QWNNPINTFI QLMSVQNQNI NADQLLLDSI NCSLTYEQLS 

       190        200        210        220        230        240 
QFVSLSQKHN ELALTFTEVI VEDLTKFQTK RHSMSQIHEV VHEHLYISTM ALINLNLTAQ 

       250        260        270        280        290        300 
AVFNPTVFDC ITAWINYISL TRSVSSSGRM DLSEIFQNLI DLMYQSTEGS DGYENAEKIL 

       310        320        330        340        350        360 
TIFGNVFAND PLLMSYDLRQ QIECIFLGVV RPDSGITDIS NKNSWMLQYM NYLVTNDFFS 

       370        380        390        400        410        420 
ELKELAICIV DFLQINTLSV CNKLFTNIQA ADNGQVQDEY IQEYIKVLLQ MTNFPLTPVL 

       430        440        450        460        470        480 
QEFFSVRMVD FWLDLSDAYT NLASETLRPN SIELSTQIFQ QLINIYLPKI SLSVKQRIIE 

       490        500        510        520        530        540 
EEGESTSVNE FEDFRNAVSD LAQSLWSILG NDNLTNVLID GMGQMPAASD ETLIIKDTDV 

       550        560        570        580        590        600 
LFRIETMCFV LNTILVDMTL SESPWIKNIV DANKFFNQNV ISVFQTGFQT SASTKVSQIL 

       610        620        630        640        650        660 
KLDFVRTSTT LIGTLAGYFK QEPFQLNPYV EALFQGLHTC TNFTSKNEQE KISNDKLEVM 

       670        680        690        700        710        720 
VIKTVSTLCE TCREELTPYL MHFISFLNTV IMPDSNVSHF TRTKLVRSIG YVVQCQVSNG 

       730        740        750        760        770        780 
PEEQAKYILQ LTNLLSGSIE HCLASSVQLQ EQQDYINCLL YCISELATSL IQPTEIIEND 

       790        800        810        820        830        840 
ALLQRLSEFQ SFWSSDPLQI RSKIMCTIDK VLDNSIYCKN SAFVEIGCLI VGKGLNLPDG 

       850        860        870        880        890        900 
EPYFLKYNMS EVMNFVLRHV PNCELATCLP YFVYLLEKLI SEFRKELTPQ EFDFMFEKIL 

       910        920        930        940        950        960 
LVYYDAYIIN DPDLLQMTIG FVNNVLDVKP GLAIGSKHWT SFILPQFLKL IPSREKFTIV 

       970        980        990       1000       1010       1020 
AVAKFWTKLI NNKKYNQEEL TTVRQQVSSI GGDLVYQIMY GLFHTQRSDL NSYTDLLRAL 

      1030       1040       1050       1060       1070       1080 
VAKFPIEARE WLVAVLPQIC NNPAGHEKFI NKLLITRGSR AAGNVILQWW LDCTTLPNYQ 


G 

« Hide

References

« Hide 'large scale' references
[1]"The DNA sequencing of the 17 kb HindIII fragment spanning the LEU1 and ATE1 loci on chromosome VII from Saccharomyces cerevisiae reveals the PDR6 gene, a new member of the genetic network controlling pleiotropic drug resistance."
Chen W., Balzi E., Capieaux E., Choder M., Goffeau A.
Yeast 7:287-299(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
Strain: ATCC 46191 / IL125-2B.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"The karyopherin Kap122p/Pdr6p imports both subunits of the transcription factor IIA into the nucleus."
Titov A.A., Blobel G.
J. Cell Biol. 147:235-246(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH NUP1; NUP2; TOA1 AND TOA2, FUNCTION.
[5]"Purification of protein A-tagged yeast ran reveals association with a novel karyopherin beta family member, Pdr6p."
Lau D., Kunzler M., Braunwarth A., Hellmuth K., Podtelejnikov A., Mann M., Hurt E.
J. Biol. Chem. 275:467-471(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH GSP2.
[6]"The yeast nuclear pore complex: composition, architecture, and transport mechanism."
Rout M.P., Aitchison J.D., Suprapto A., Hjertaas K., Zhao Y., Chait B.T.
J. Cell Biol. 148:635-651(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, ASSOCIATION WITH THE NUCLEAR PORE COMPLEX.
[7]"Nuclear localization of the Saccharomyces cerevisiae ribonucleotide reductase small subunit requires a karyopherin and a WD40 repeat protein."
Zhang Z., An X., Yang K., Perlstein D.L., Hicks L., Kelleher N., Stubbe J., Huang M.
Proc. Natl. Acad. Sci. U.S.A. 103:1422-1427(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH WTM1.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Dif1 controls subcellular localization of ribonucleotide reductase by mediating nuclear import of the R2 subunit."
Wu X., Huang M.
Mol. Cell. Biol. 28:7156-7167(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S58126 Genomic DNA. Translation: AAD13901.1.
S57895 Genomic DNA. Translation: AAB19613.1.
Z72538 Genomic DNA. Translation: CAA96716.1.
BK006941 Genomic DNA. Translation: DAA08082.1.
PIRS15040.
RefSeqNP_011499.1. NM_001180881.1.

3D structure databases

ProteinModelPortalP32767.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33230. 88 interactions.
DIPDIP-2449N.
IntActP32767. 13 interactions.
MINTMINT-707770.
STRING4932.YGL016W.

Proteomic databases

MaxQBP32767.
PaxDbP32767.
PeptideAtlasP32767.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL016W; YGL016W; YGL016W.
GeneID852868.
KEGGsce:YGL016W.

Organism-specific databases

CYGDYGL016w.
SGDS000002984. KAP122.

Phylogenomic databases

eggNOGNOG260621.
OMAHLYISTM.
OrthoDBEOG7XSTP0.

Enzyme and pathway databases

BioCycYEAST:G3O-30537-MONOMER.

Gene expression databases

GenevestigatorP32767.

Family and domain databases

InterProIPR016024. ARM-type_fold.
[Graphical view]
SUPFAMSSF48371. SSF48371. 3 hits.
ProtoNetSearch...

Other

NextBio972490.

Entry information

Entry nameKA122_YEAST
AccessionPrimary (citable) accession number: P32767
Secondary accession number(s): D6VUC1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 14, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families