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Protein

Cystatin-8

Gene

Cst8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Performs a specialized role during sperm development and maturation.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Thiol protease inhibitor

Protein family/group databases

MEROPSiI25.027.

Names & Taxonomyi

Protein namesi
Recommended name:
Cystatin-8
Alternative name(s):
Cystatin-related epididymal spermatogenic protein
Cystatin-related epididymal-specific protein
Gene namesi
Name:Cst8
Synonyms:Cres
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:107161. Cst8.

Subcellular locationi

GO - Cellular componenti

  • cell surface Source: MGI
  • cytoplasm Source: MGI
  • extracellular region Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Chaini20 – 142123Cystatin-8PRO_0000006654Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi39 – 391N-linked (GlcNAc...)Sequence analysis
Disulfide bondi95 ↔ 105By similarity
Glycosylationi100 – 1001N-linked (GlcNAc...)Sequence analysis
Disulfide bondi119 ↔ 139By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP32766.
PRIDEiP32766.

PTM databases

PhosphoSiteiP32766.

Expressioni

Tissue specificityi

Proximal caput region of the epididymis. Lower expression in the testis. Within the testis it is localized to the elongating spermatids, whereas within the epididymis it is exclusively synthesized by the proximal caput epithelium.

Inductioni

Testicular factors or hormones other than androgens present in the testicular fluid may be involved in the regulation of CRES gene expression.

Gene expression databases

BgeeiP32766.
GenevisibleiP32766. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000028931.

Structurei

3D structure databases

ProteinModelPortaliP32766.
SMRiP32766. Positions 39-141.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi77 – 815Secondary area of contactSequence analysis

Sequence similaritiesi

Belongs to the cystatin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IXH4. Eukaryota.
ENOG41113F6. LUCA.
GeneTreeiENSGT00840000129787.
HOGENOMiHOG000231754.
HOVERGENiHBG095685.
InParanoidiP32766.
KOiK13904.
OMAiKQCLWFA.
OrthoDBiEOG7M98J9.
PhylomeDBiP32766.

Family and domain databases

InterProiIPR027214. Cystatin.
IPR000010. Cystatin_dom.
[Graphical view]
PANTHERiPTHR11413. PTHR11413. 1 hit.
PfamiPF00031. Cystatin. 1 hit.
[Graphical view]
SMARTiSM00043. CY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32766-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKPLWLSLI LFIIPVALAV GVDQSKNEVK AQNYFGSINI SNANVKQCVW
60 70 80 90 100
FAMKEYNKES EDKYVFLVDK ILHAKLQITD RMEYQIDVQI SRSNCKKPLN
110 120 130 140
NTENCIPQKK PELEKKMSCS FLVGALPWNG EFNLLSKECK DV
Length:142
Mass (Da):16,288
Last modified:May 30, 2000 - v2
Checksum:i50B446E98F66673E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 1512PLWLS…LFIIP → GTRDEQVGESQK in AAC35390 (PubMed:1280328).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF091503 Genomic DNA. Translation: AAC61754.1.
AK018943 mRNA. Translation: BAB31489.1.
AK054409 mRNA. Translation: BAC35768.1.
AK161808 mRNA. Translation: BAE36585.1.
BC049753 mRNA. Translation: AAH49753.1.
AF090691 mRNA. Translation: AAC36316.1.
S49926 mRNA. Translation: AAC35390.1.
CCDSiCCDS16848.1.
PIRiA45361.
RefSeqiNP_034108.1. NM_009978.2.
XP_006498721.1. XM_006498658.2.
XP_006498722.1. XM_006498659.2.
UniGeneiMm.20435.

Genome annotation databases

EnsembliENSMUST00000028931; ENSMUSP00000028931; ENSMUSG00000027442.
ENSMUST00000109947; ENSMUSP00000105573; ENSMUSG00000027442.
GeneIDi13012.
KEGGimmu:13012.
UCSCiuc008mto.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF091503 Genomic DNA. Translation: AAC61754.1.
AK018943 mRNA. Translation: BAB31489.1.
AK054409 mRNA. Translation: BAC35768.1.
AK161808 mRNA. Translation: BAE36585.1.
BC049753 mRNA. Translation: AAH49753.1.
AF090691 mRNA. Translation: AAC36316.1.
S49926 mRNA. Translation: AAC35390.1.
CCDSiCCDS16848.1.
PIRiA45361.
RefSeqiNP_034108.1. NM_009978.2.
XP_006498721.1. XM_006498658.2.
XP_006498722.1. XM_006498659.2.
UniGeneiMm.20435.

3D structure databases

ProteinModelPortaliP32766.
SMRiP32766. Positions 39-141.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000028931.

Protein family/group databases

MEROPSiI25.027.

PTM databases

PhosphoSiteiP32766.

Proteomic databases

PaxDbiP32766.
PRIDEiP32766.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028931; ENSMUSP00000028931; ENSMUSG00000027442.
ENSMUST00000109947; ENSMUSP00000105573; ENSMUSG00000027442.
GeneIDi13012.
KEGGimmu:13012.
UCSCiuc008mto.1. mouse.

Organism-specific databases

CTDi10047.
MGIiMGI:107161. Cst8.

Phylogenomic databases

eggNOGiENOG410IXH4. Eukaryota.
ENOG41113F6. LUCA.
GeneTreeiENSGT00840000129787.
HOGENOMiHOG000231754.
HOVERGENiHBG095685.
InParanoidiP32766.
KOiK13904.
OMAiKQCLWFA.
OrthoDBiEOG7M98J9.
PhylomeDBiP32766.

Miscellaneous databases

PROiP32766.
SOURCEiSearch...

Gene expression databases

BgeeiP32766.
GenevisibleiP32766. MM.

Family and domain databases

InterProiIPR027214. Cystatin.
IPR000010. Cystatin_dom.
[Graphical view]
PANTHERiPTHR11413. PTHR11413. 1 hit.
PfamiPF00031. Cystatin. 1 hit.
[Graphical view]
SMARTiSM00043. CY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure, alternative splicing and chromosomal localization of the cystatin-related epididymal spermatogenic gene."
    Cornwall G.A., Hsia N., Sutton H.G.
    Biochem. J. 340:85-93(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: C3H/HeJ and CD-1.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  4. "The CRES gene: a unique testis-regulated gene related to the cystatin family is highly restricted in its expression to the proximal region of the mouse epididymis."
    Cornwall G.A., Orgebin-Crist M.-C., Hann S.R.
    Mol. Endocrinol. 6:1653-1664(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-142.
    Tissue: Epididymis.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.

Entry informationi

Entry nameiCST8_MOUSE
AccessioniPrimary (citable) accession number: P32766
Secondary accession number(s): O89102, Q543A7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: May 30, 2000
Last modified: June 8, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.