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Protein

Choline O-acetyltransferase

Gene

cha-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

Catalytic activityi

Acetyl-CoA + choline = CoA + O-acetylcholine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei341 – 3411Proton acceptorBy similarity
Binding sitei456 – 4561Coenzyme ABy similarity
Binding sitei559 – 5591Coenzyme ABy similarity

GO - Molecular functioni

  • choline O-acetyltransferase activity Source: WormBase

GO - Biological processi

  • acetylcholine biosynthetic process Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Neurotransmitter biosynthesis

Enzyme and pathway databases

ReactomeiREACT_287639. Synthesis of PC.
REACT_324251. Acetylcholine Neurotransmitter Release Cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Choline O-acetyltransferase (EC:2.3.1.6)
Short name:
CHOACTase
Short name:
ChAT
Short name:
Choline acetylase
Gene namesi
Name:cha-1
ORF Names:ZC416.8
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiZC416.8b; CE17308; WBGene00000481; cha-1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: WormBase
  • excitatory synapse Source: WormBase
  • neuronal cell body Source: WormBase
  • neuron projection Source: WormBase
  • organelle membrane Source: WormBase
  • plasma membrane Source: WormBase
  • synapse Source: WormBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 627627Choline O-acetyltransferasePRO_0000210153Add
BLAST

Proteomic databases

PaxDbiP32756.
PRIDEiP32756.

Interactioni

Protein-protein interaction databases

STRINGi6239.ZC416.8b.

Structurei

3D structure databases

ProteinModelPortaliP32756.
SMRiP32756. Positions 16-627.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni418 – 43013Coenzyme A bindingBy similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG70127.
GeneTreeiENSGT00760000119220.
HOGENOMiHOG000233845.
InParanoidiP32756.
OMAiRRFRYGR.
PhylomeDBiP32756.

Family and domain databases

InterProiIPR000542. Carn_acyl_trans.
[Graphical view]
PANTHERiPTHR22589. PTHR22589. 1 hit.
PfamiPF00755. Carn_acyltransf. 1 hit.
[Graphical view]
PROSITEiPS00439. ACYLTRANSF_C_1. 1 hit.
PS00440. ACYLTRANSF_C_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32756-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKEKVDELP PNDNWYETAL PKPPVPSLEA TLDRYLEYAA VVAVGQKASL
60 70 80 90 100
ATTHDAAHKF VRQATPLQEQ LLEIAEKSPN WATKFWLPEM YMRVRMPTPV
110 120 130 140 150
NSNPGYIFPK VKFETKEDHI KYTALLTRGL LEYKNLIDTK QVCREKSTGA
160 170 180 190 200
QKLQMCMEQY DRVLSCYREP GVGEDTQIRK QKTNDGNEHV LVMCRNQTFL
210 220 230 240 250
LHSRINGALV SYADVEYQLA QIEEISKINQ NNTANIGASG VGPRDNAALF
260 270 280 290 300
WQDMLTVEQN SKSYEWVKSA LFVVCLDMED PIDYGKNDTM SISEKEKEFV
310 320 330 340 350
ARGYSTLTGH GSSKFGLNRW YDATIQLVVS SSGVNGLCIE HSTAEGIVII
360 370 380 390 400
NMAETAIRYA QKYFKSKMVW NDVRNVHPKS LTWHFSENSR NILKKQAEVF
410 420 430 440 450
DELANELELE VLIFNEFGKD SIKNWRVSPD GFIQLIMQLA HYKTHGHLVS
460 470 480 490 500
TYESASVRRF GAGRVDNIRA NTQEALEWVT AMASKKESKE RKLELFKKAV
510 520 530 540 550
LKQVKVTLEN ISGYGVDNHL CALFCLARER EETTGEDIPS LFLDPLWSEV
560 570 580 590 600
MRFPLSTSQV TTSLDIPDCY LTYGAVVRDG YGCPYNIQPD RVIFAPTAFR
610 620
SDPRTDLQHF KKSLAGAMRD VKELLSN
Length:627
Mass (Da):71,317
Last modified:October 1, 1993 - v1
Checksum:i59F52175E6F16494
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08969 mRNA. Translation: AAA53659.1.
L08970 Genomic DNA. Translation: AAA53660.1.
FO080977 Genomic DNA. Translation: CCD68242.1.
PIRiT37293.
RefSeqiNP_001023603.1. NM_001028432.2.
UniGeneiCel.16989.

Genome annotation databases

EnsemblMetazoaiZC416.8b; ZC416.8b; WBGene00000481.
GeneIDi24105307.
KEGGicel:cha-1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08969 mRNA. Translation: AAA53659.1.
L08970 Genomic DNA. Translation: AAA53660.1.
FO080977 Genomic DNA. Translation: CCD68242.1.
PIRiT37293.
RefSeqiNP_001023603.1. NM_001028432.2.
UniGeneiCel.16989.

3D structure databases

ProteinModelPortaliP32756.
SMRiP32756. Positions 16-627.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.ZC416.8b.

Proteomic databases

PaxDbiP32756.
PRIDEiP32756.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiZC416.8b; ZC416.8b; WBGene00000481.
GeneIDi24105307.
KEGGicel:cha-1.

Organism-specific databases

WormBaseiZC416.8b; CE17308; WBGene00000481; cha-1.

Phylogenomic databases

eggNOGiNOG70127.
GeneTreeiENSGT00760000119220.
HOGENOMiHOG000233845.
InParanoidiP32756.
OMAiRRFRYGR.
PhylomeDBiP32756.

Enzyme and pathway databases

ReactomeiREACT_287639. Synthesis of PC.
REACT_324251. Acetylcholine Neurotransmitter Release Cycle.

Miscellaneous databases

NextBioi895448.
PROiP32756.

Family and domain databases

InterProiIPR000542. Carn_acyl_trans.
[Graphical view]
PANTHERiPTHR22589. PTHR22589. 1 hit.
PfamiPF00755. Carn_acyltransf. 1 hit.
[Graphical view]
PROSITEiPS00439. ACYLTRANSF_C_1. 1 hit.
PS00440. ACYLTRANSF_C_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the choline acetyltransferase structural gene (cha-1) from C. elegans."
    Alfonso A., Grundahl K., McManus J.R., Rand J.B.
    J. Neurosci. 14:2290-2300(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiCLAT_CAEEL
AccessioniPrimary (citable) accession number: P32756
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: July 22, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.