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P32756

- CLAT_CAEEL

UniProt

P32756 - CLAT_CAEEL

Protein

Choline O-acetyltransferase

Gene

cha-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

    Catalytic activityi

    Acetyl-CoA + choline = CoA + O-acetylcholine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei341 – 3411Proton acceptorBy similarity
    Binding sitei456 – 4561Coenzyme ABy similarity
    Binding sitei559 – 5591Coenzyme ABy similarity

    GO - Molecular functioni

    1. choline O-acetyltransferase activity Source: WormBase

    GO - Biological processi

    1. acetylcholine biosynthetic process Source: WormBase

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Neurotransmitter biosynthesis

    Enzyme and pathway databases

    ReactomeiREACT_183754. Synthesis of PC.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Choline O-acetyltransferase (EC:2.3.1.6)
    Short name:
    CHOACTase
    Short name:
    ChAT
    Short name:
    Choline acetylase
    Gene namesi
    Name:cha-1
    ORF Names:ZC416.8
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome IV

    Organism-specific databases

    WormBaseiZC416.8b; CE17308; WBGene00000481; cha-1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: WormBase
    2. excitatory synapse Source: WormBase
    3. neuronal cell body Source: WormBase
    4. neuron projection Source: WormBase
    5. organelle membrane Source: WormBase
    6. plasma membrane Source: WormBase
    7. synapse Source: WormBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 627627Choline O-acetyltransferasePRO_0000210153Add
    BLAST

    Proteomic databases

    PaxDbiP32756.
    PRIDEiP32756.

    Interactioni

    Protein-protein interaction databases

    STRINGi6239.ZC416.8b.

    Structurei

    3D structure databases

    ProteinModelPortaliP32756.
    SMRiP32756. Positions 16-627.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni418 – 43013Coenzyme A bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG70127.
    GeneTreeiENSGT00740000115446.
    HOGENOMiHOG000233845.
    OMAiFCLARER.
    PhylomeDBiP32756.

    Family and domain databases

    InterProiIPR000542. Carn_acyl_trans.
    [Graphical view]
    PANTHERiPTHR22589. PTHR22589. 1 hit.
    PfamiPF00755. Carn_acyltransf. 1 hit.
    [Graphical view]
    PROSITEiPS00439. ACYLTRANSF_C_1. 1 hit.
    PS00440. ACYLTRANSF_C_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32756-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEKEKVDELP PNDNWYETAL PKPPVPSLEA TLDRYLEYAA VVAVGQKASL    50
    ATTHDAAHKF VRQATPLQEQ LLEIAEKSPN WATKFWLPEM YMRVRMPTPV 100
    NSNPGYIFPK VKFETKEDHI KYTALLTRGL LEYKNLIDTK QVCREKSTGA 150
    QKLQMCMEQY DRVLSCYREP GVGEDTQIRK QKTNDGNEHV LVMCRNQTFL 200
    LHSRINGALV SYADVEYQLA QIEEISKINQ NNTANIGASG VGPRDNAALF 250
    WQDMLTVEQN SKSYEWVKSA LFVVCLDMED PIDYGKNDTM SISEKEKEFV 300
    ARGYSTLTGH GSSKFGLNRW YDATIQLVVS SSGVNGLCIE HSTAEGIVII 350
    NMAETAIRYA QKYFKSKMVW NDVRNVHPKS LTWHFSENSR NILKKQAEVF 400
    DELANELELE VLIFNEFGKD SIKNWRVSPD GFIQLIMQLA HYKTHGHLVS 450
    TYESASVRRF GAGRVDNIRA NTQEALEWVT AMASKKESKE RKLELFKKAV 500
    LKQVKVTLEN ISGYGVDNHL CALFCLARER EETTGEDIPS LFLDPLWSEV 550
    MRFPLSTSQV TTSLDIPDCY LTYGAVVRDG YGCPYNIQPD RVIFAPTAFR 600
    SDPRTDLQHF KKSLAGAMRD VKELLSN 627
    Length:627
    Mass (Da):71,317
    Last modified:October 1, 1993 - v1
    Checksum:i59F52175E6F16494
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L08969 mRNA. Translation: AAA53659.1.
    L08970 Genomic DNA. Translation: AAA53660.1.
    FO080977 Genomic DNA. Translation: CCD68242.1.
    PIRiT37293.
    RefSeqiNP_001023603.1. NM_001028432.2.
    UniGeneiCel.16989.

    Genome annotation databases

    EnsemblMetazoaiZC416.8b; ZC416.8b; WBGene00000481.
    GeneIDi13192683.
    KEGGicel:CELE_ZC416.8b.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L08969 mRNA. Translation: AAA53659.1 .
    L08970 Genomic DNA. Translation: AAA53660.1 .
    FO080977 Genomic DNA. Translation: CCD68242.1 .
    PIRi T37293.
    RefSeqi NP_001023603.1. NM_001028432.2.
    UniGenei Cel.16989.

    3D structure databases

    ProteinModelPortali P32756.
    SMRi P32756. Positions 16-627.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 6239.ZC416.8b.

    Proteomic databases

    PaxDbi P32756.
    PRIDEi P32756.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai ZC416.8b ; ZC416.8b ; WBGene00000481 .
    GeneIDi 13192683.
    KEGGi cel:CELE_ZC416.8b.

    Organism-specific databases

    CTDi 13192683.
    WormBasei ZC416.8b ; CE17308 ; WBGene00000481 ; cha-1.

    Phylogenomic databases

    eggNOGi NOG70127.
    GeneTreei ENSGT00740000115446.
    HOGENOMi HOG000233845.
    OMAi FCLARER.
    PhylomeDBi P32756.

    Enzyme and pathway databases

    Reactomei REACT_183754. Synthesis of PC.

    Miscellaneous databases

    NextBioi 895448.
    PROi P32756.

    Family and domain databases

    InterProi IPR000542. Carn_acyl_trans.
    [Graphical view ]
    PANTHERi PTHR22589. PTHR22589. 1 hit.
    Pfami PF00755. Carn_acyltransf. 1 hit.
    [Graphical view ]
    PROSITEi PS00439. ACYLTRANSF_C_1. 1 hit.
    PS00440. ACYLTRANSF_C_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the choline acetyltransferase structural gene (cha-1) from C. elegans."
      Alfonso A., Grundahl K., McManus J.R., Rand J.B.
      J. Neurosci. 14:2290-2300(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: Bristol N2.
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.

    Entry informationi

    Entry nameiCLAT_CAEEL
    AccessioniPrimary (citable) accession number: P32756
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3