SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P32756

- CLAT_CAEEL

UniProt

P32756 - CLAT_CAEEL

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Choline O-acetyltransferase

Gene
cha-1, ZC416.8
Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

Catalytic activityi

Acetyl-CoA + choline = CoA + O-acetylcholine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei341 – 3411Proton acceptor By similarity
Binding sitei456 – 4561Coenzyme A By similarity
Binding sitei559 – 5591Coenzyme A By similarity

GO - Molecular functioni

  1. choline O-acetyltransferase activity Source: WormBase

GO - Biological processi

  1. acetylcholine biosynthetic process Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Neurotransmitter biosynthesis

Enzyme and pathway databases

ReactomeiREACT_183754. Synthesis of PC.

Names & Taxonomyi

Protein namesi
Recommended name:
Choline O-acetyltransferase (EC:2.3.1.6)
Short name:
CHOACTase
Short name:
ChAT
Short name:
Choline acetylase
Gene namesi
Name:cha-1
ORF Names:ZC416.8
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome IV

Organism-specific databases

WormBaseiZC416.8b; CE17308; WBGene00000481; cha-1.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: WormBase
  2. excitatory synapse Source: WormBase
  3. neuronal cell body Source: WormBase
  4. neuron projection Source: WormBase
  5. organelle membrane Source: WormBase
  6. plasma membrane Source: WormBase
  7. synapse Source: WormBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 627627Choline O-acetyltransferasePRO_0000210153Add
BLAST

Proteomic databases

PaxDbiP32756.
PRIDEiP32756.

Interactioni

Protein-protein interaction databases

STRINGi6239.ZC416.8b.

Structurei

3D structure databases

ProteinModelPortaliP32756.
SMRiP32756. Positions 16-627.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni418 – 43013Coenzyme A binding By similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG70127.
GeneTreeiENSGT00740000115446.
HOGENOMiHOG000233845.
OMAiFCLARER.
PhylomeDBiP32756.

Family and domain databases

InterProiIPR000542. Carn_acyl_trans.
[Graphical view]
PANTHERiPTHR22589. PTHR22589. 1 hit.
PfamiPF00755. Carn_acyltransf. 1 hit.
[Graphical view]
PROSITEiPS00439. ACYLTRANSF_C_1. 1 hit.
PS00440. ACYLTRANSF_C_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32756-1 [UniParc]FASTAAdd to Basket

« Hide

MEKEKVDELP PNDNWYETAL PKPPVPSLEA TLDRYLEYAA VVAVGQKASL    50
ATTHDAAHKF VRQATPLQEQ LLEIAEKSPN WATKFWLPEM YMRVRMPTPV 100
NSNPGYIFPK VKFETKEDHI KYTALLTRGL LEYKNLIDTK QVCREKSTGA 150
QKLQMCMEQY DRVLSCYREP GVGEDTQIRK QKTNDGNEHV LVMCRNQTFL 200
LHSRINGALV SYADVEYQLA QIEEISKINQ NNTANIGASG VGPRDNAALF 250
WQDMLTVEQN SKSYEWVKSA LFVVCLDMED PIDYGKNDTM SISEKEKEFV 300
ARGYSTLTGH GSSKFGLNRW YDATIQLVVS SSGVNGLCIE HSTAEGIVII 350
NMAETAIRYA QKYFKSKMVW NDVRNVHPKS LTWHFSENSR NILKKQAEVF 400
DELANELELE VLIFNEFGKD SIKNWRVSPD GFIQLIMQLA HYKTHGHLVS 450
TYESASVRRF GAGRVDNIRA NTQEALEWVT AMASKKESKE RKLELFKKAV 500
LKQVKVTLEN ISGYGVDNHL CALFCLARER EETTGEDIPS LFLDPLWSEV 550
MRFPLSTSQV TTSLDIPDCY LTYGAVVRDG YGCPYNIQPD RVIFAPTAFR 600
SDPRTDLQHF KKSLAGAMRD VKELLSN 627
Length:627
Mass (Da):71,317
Last modified:October 1, 1993 - v1
Checksum:i59F52175E6F16494
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L08969 mRNA. Translation: AAA53659.1.
L08970 Genomic DNA. Translation: AAA53660.1.
FO080977 Genomic DNA. Translation: CCD68242.1.
PIRiT37293.
RefSeqiNP_001023603.1. NM_001028432.2.
UniGeneiCel.16989.

Genome annotation databases

EnsemblMetazoaiZC416.8b; ZC416.8b; WBGene00000481.
GeneIDi13192683.
KEGGicel:CELE_ZC416.8b.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L08969 mRNA. Translation: AAA53659.1 .
L08970 Genomic DNA. Translation: AAA53660.1 .
FO080977 Genomic DNA. Translation: CCD68242.1 .
PIRi T37293.
RefSeqi NP_001023603.1. NM_001028432.2.
UniGenei Cel.16989.

3D structure databases

ProteinModelPortali P32756.
SMRi P32756. Positions 16-627.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 6239.ZC416.8b.

Proteomic databases

PaxDbi P32756.
PRIDEi P32756.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai ZC416.8b ; ZC416.8b ; WBGene00000481 .
GeneIDi 13192683.
KEGGi cel:CELE_ZC416.8b.

Organism-specific databases

CTDi 13192683.
WormBasei ZC416.8b ; CE17308 ; WBGene00000481 ; cha-1.

Phylogenomic databases

eggNOGi NOG70127.
GeneTreei ENSGT00740000115446.
HOGENOMi HOG000233845.
OMAi FCLARER.
PhylomeDBi P32756.

Enzyme and pathway databases

Reactomei REACT_183754. Synthesis of PC.

Miscellaneous databases

NextBioi 895448.
PROi P32756.

Family and domain databases

InterProi IPR000542. Carn_acyl_trans.
[Graphical view ]
PANTHERi PTHR22589. PTHR22589. 1 hit.
Pfami PF00755. Carn_acyltransf. 1 hit.
[Graphical view ]
PROSITEi PS00439. ACYLTRANSF_C_1. 1 hit.
PS00440. ACYLTRANSF_C_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the choline acetyltransferase structural gene (cha-1) from C. elegans."
    Alfonso A., Grundahl K., McManus J.R., Rand J.B.
    J. Neurosci. 14:2290-2300(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiCLAT_CAEEL
AccessioniPrimary (citable) accession number: P32756
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: September 3, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi