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Protein

Choline O-acetyltransferase

Gene

cha-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses (By similarity). Required in SIA sublateral cholinergic motor neurons for a left-right turning behavior that occurs during the lethargus phase of the normal sleep process called "flipping" (PubMed:28244369). During "flipping" animals rotate 180 degrees about their longitudinal axis (PubMed:28244369).By similarity1 Publication

Catalytic activityi

Acetyl-CoA + choline = CoA + O-acetylcholine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei341Proton acceptorBy similarity1
Binding sitei456Coenzyme ABy similarity1
Binding sitei559Coenzyme ABy similarity1

GO - Molecular functioni

  • choline O-acetyltransferase activity Source: WormBase

GO - Biological processi

  • acetylcholine biosynthetic process Source: WormBase

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processNeurotransmitter biosynthesis

Enzyme and pathway databases

ReactomeiR-CEL-1483191. Synthesis of PC.
R-CEL-264642. Acetylcholine Neurotransmitter Release Cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Choline O-acetyltransferase (EC:2.3.1.6By similarity)
Short name:
CHOACTase
Short name:
ChAT
Short name:
Choline acetylase
Gene namesi
Name:cha-1Imported
ORF Names:ZC416.8Imported
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiZC416.8b; CE17308; WBGene00000481; cha-1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: WormBase
  • excitatory synapse Source: WormBase
  • neuronal cell body Source: WormBase
  • neuron projection Source: WormBase
  • organelle membrane Source: WormBase
  • plasma membrane Source: WormBase
  • synapse Source: WormBase

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown in SIA sublateral motor neurons results in abnormal sleep behavior whereby larvae display a defect in "flipping," which is a left-right turning behavior that occurs during sleep.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002101531 – 627Choline O-acetyltransferaseAdd BLAST627

Proteomic databases

EPDiP32756.
PaxDbiP32756.
PeptideAtlasiP32756.

Expressioni

Tissue specificityi

Expressed in SIA, SIB and SMB sublateral motor neurons.1 Publication

Gene expression databases

BgeeiWBGene00000481.

Interactioni

Protein-protein interaction databases

STRINGi6239.ZC416.8b.

Structurei

3D structure databases

ProteinModelPortaliP32756.
SMRiP32756.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni418 – 430Coenzyme A bindingBy similarityAdd BLAST13

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3717. Eukaryota.
ENOG410XNZ9. LUCA.
GeneTreeiENSGT00760000119220.
HOGENOMiHOG000233845.
InParanoidiP32756.
KOiK00623.
OMAiDKPMQFV.
OrthoDBiEOG091G038F.
PhylomeDBiP32756.

Family and domain databases

InterProiView protein in InterPro
IPR000542. Carn_acyl_trans.
PANTHERiPTHR22589. PTHR22589. 1 hit.
PfamiView protein in Pfam
PF00755. Carn_acyltransf. 1 hit.
PROSITEiView protein in PROSITE
PS00439. ACYLTRANSF_C_1. 1 hit.
PS00440. ACYLTRANSF_C_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P32756-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKEKVDELP PNDNWYETAL PKPPVPSLEA TLDRYLEYAA VVAVGQKASL
60 70 80 90 100
ATTHDAAHKF VRQATPLQEQ LLEIAEKSPN WATKFWLPEM YMRVRMPTPV
110 120 130 140 150
NSNPGYIFPK VKFETKEDHI KYTALLTRGL LEYKNLIDTK QVCREKSTGA
160 170 180 190 200
QKLQMCMEQY DRVLSCYREP GVGEDTQIRK QKTNDGNEHV LVMCRNQTFL
210 220 230 240 250
LHSRINGALV SYADVEYQLA QIEEISKINQ NNTANIGASG VGPRDNAALF
260 270 280 290 300
WQDMLTVEQN SKSYEWVKSA LFVVCLDMED PIDYGKNDTM SISEKEKEFV
310 320 330 340 350
ARGYSTLTGH GSSKFGLNRW YDATIQLVVS SSGVNGLCIE HSTAEGIVII
360 370 380 390 400
NMAETAIRYA QKYFKSKMVW NDVRNVHPKS LTWHFSENSR NILKKQAEVF
410 420 430 440 450
DELANELELE VLIFNEFGKD SIKNWRVSPD GFIQLIMQLA HYKTHGHLVS
460 470 480 490 500
TYESASVRRF GAGRVDNIRA NTQEALEWVT AMASKKESKE RKLELFKKAV
510 520 530 540 550
LKQVKVTLEN ISGYGVDNHL CALFCLARER EETTGEDIPS LFLDPLWSEV
560 570 580 590 600
MRFPLSTSQV TTSLDIPDCY LTYGAVVRDG YGCPYNIQPD RVIFAPTAFR
610 620
SDPRTDLQHF KKSLAGAMRD VKELLSN
Length:627
Mass (Da):71,317
Last modified:October 1, 1993 - v1
Checksum:i59F52175E6F16494
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08969 mRNA. Translation: AAA53659.1.
L08970 Genomic DNA. Translation: AAA53660.1.
FO080977 Genomic DNA. Translation: CCD68242.1.
PIRiT37293.
RefSeqiNP_001023603.1. NM_001028432.2.
UniGeneiCel.16989.

Genome annotation databases

EnsemblMetazoaiZC416.8b; ZC416.8b; WBGene00000481.
GeneIDi24105307.
KEGGicel:CELE_cha-1.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiCLAT_CAEEL
AccessioniPrimary (citable) accession number: P32756
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: July 5, 2017
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families