P32753 (CHLE_SHEEP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cholinesterase
EC=3.1.1.8

Alternative name(s):
Acylcholine acylhydrolase
Butyrylcholine esterase
Choline esterase II
Pseudocholinesterase

Gene names

Name:

BCHE

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	141 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters By similarity.
Catalytic activity	An acylcholine + H2O = choline + a carboxylate.
Subunit structure	Homotetramer; disulfide-linked. Dimer of dimers By similarity.
Subcellular location	Secreted By similarity.
Tissue specificity	Present in most cells except erythrocytes.
Sequence similarities	Belongs to the type-B carboxylesterase/lipase family.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Hydrolase Serine esterase
PTM	Disulfide bond Glycoprotein Phosphoprotein
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	acetylcholinesterase activity Inferred from sequence or structural similarity. Source: UniProtKB carboxylesterase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›141	›141	Cholinesterase		PRO_0000070288
Regions
	Region	49 – 50	2	Substrate binding By similarity
Sites
	Active site	131	1	Acyl-ester intermediate By similarity
Amino acid modifications
	Modified residue	131	1	Phosphoserine By similarity
	Glycosylation	39	1	N-linked (GlcNAc...) Potential
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	141	1

Sequences

	Sequence		Length	Mass (Da)
	P32753 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 5FDFAA11730564D3	FASTA	141	15,234
	10 20 30 40 50 60 NTDQSFPGFL GSEMWNPNTD LSEDCLYLNV WIPTPKPKNA TVMIWIYGGS FQTGTSSLHV 70 80 90 100 110 120 YDGKFLARVE RVIVVSMNYR VGALGFLALP GNPEAPGNVG LFDQQLALQW VQKNIAAFGG 130 140 NPKSVTLFGE SAGAASVSLH L

References

[1]

"Use of the polymerase chain reaction for homology probing of butyrylcholinesterase from several vertebrates." Arpagaus M., Chatonnet A., Masson P., Newton M., Vaughan T.A., Bartels C.F., Nogueira C.P., la Du B.N., Lockridge O. J. Biol. Chem. 266:6966-6974(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M62780 Genomic DNA. Translation: AAA31509.1.
PIR	B39768.
3D structure databases
ProteinModelPortal	P32753.
SMR	P32753. Positions 1-141.
ModBase	Search...
Protein family/group databases
MEROPS	S09.980.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG106132.
Family and domain databases
InterPro	IPR002018. CarbesteraseB. IPR019826. Carboxylesterase_B_AS. IPR019819. Carboxylesterase_B_CS. IPR000997. Cholinesterase. [Graphical view]
Pfam	PF00135. COesterase. 1 hit. [Graphical view]
PRINTS	PR00878. CHOLNESTRASE.
PROSITE	PS00122. CARBOXYLESTERASE_B_1. 1 hit. PS00941. CARBOXYLESTERASE_B_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHLE_SHEEP

Accession

Primary (citable) accession number: P32753

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	April 3, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program