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P32749

- CHLE_BOVIN

UniProt

P32749 - CHLE_BOVIN

Protein

Cholinesterase

Gene

BCHE

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 2 (03 Apr 2007)
      Previous versions | rss
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    Functioni

    Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters By similarity.By similarity

    Catalytic activityi

    An acylcholine + H2O = choline + a carboxylate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei226 – 2261Acyl-ester intermediatePROSITE-ProRule annotation
    Active sitei353 – 3531Charge relay systemBy similarity
    Active sitei466 – 4661Charge relay systemBy similarity

    GO - Molecular functioni

    1. acetylcholinesterase activity Source: UniProtKB
    2. cholinesterase activity Source: UniProtKB

    GO - Biological processi

    1. choline metabolic process Source: RefGenome
    2. synaptic transmission, cholinergic Source: RefGenome

    Keywords - Molecular functioni

    Hydrolase, Serine esterase

    Enzyme and pathway databases

    ReactomeiREACT_223503. Synthesis, secretion, and deacylation of Ghrelin.

    Protein family/group databases

    MEROPSiS09.980.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cholinesterase (EC:3.1.1.8)
    Alternative name(s):
    Acylcholine acylhydrolase
    Butyrylcholine esterase
    Choline esterase II
    Pseudocholinesterase
    Gene namesi
    Name:BCHE
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 1

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum lumen Source: RefGenome
    2. extracellular space Source: RefGenome
    3. nuclear envelope lumen Source: Ensembl

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2828Sequence AnalysisAdd
    BLAST
    Chaini29 – 602574CholinesterasePRO_0000070283Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi85 – 851N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi134 – 1341N-linked (GlcNAc...)Sequence Analysis
    Modified residuei226 – 2261PhosphoserineBy similarity
    Glycosylationi269 – 2691N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi284 – 2841N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi369 – 3691N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi483 – 4831N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi509 – 5091N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi513 – 5131N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi514 – 5141N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PRIDEiP32749.

    Expressioni

    Tissue specificityi

    Present in most cells except erythrocytes.

    Interactioni

    Subunit structurei

    Homotetramer; disulfide-linked. Dimer of dimers By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000014794.

    Structurei

    3D structure databases

    ProteinModelPortaliP32749.
    SMRiP32749. Positions 32-562, 564-593.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni144 – 1452Substrate bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2272.
    GeneTreeiENSGT00740000115241.
    HOGENOMiHOG000091866.
    HOVERGENiHBG008839.
    InParanoidiP32749.
    KOiK01050.
    OMAiWIPTPKP.
    OrthoDBiEOG789C9R.
    TreeFamiTF315470.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR014788. AChE_tetra.
    IPR002018. CarbesteraseB.
    IPR019826. Carboxylesterase_B_AS.
    IPR019819. Carboxylesterase_B_CS.
    IPR000997. Cholinesterase.
    [Graphical view]
    PfamiPF08674. AChE_tetra. 1 hit.
    PF00135. COesterase. 1 hit.
    [Graphical view]
    PRINTSiPR00878. CHOLNESTRASE.
    ProDomiPD415333. AChE_tetra. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF53474. SSF53474. 1 hit.
    PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
    PS00941. CARBOXYLESTERASE_B_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P32749-1 [UniParc]FASTAAdd to Basket

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    MQSRSTVIYI RFVLWFLLLW VLFEKSHTEE DIIITTKNGK VRGMHLPVLG    50
    GTVTAFLGIP YAQPPLGRLR FKKPQSLTKW PDIWNATKYA NSCYQNTDQS 100
    FPGFLGSEMW NPNTDLSEDC LYLNVWIPTP KPKNATVMIW IYGGSFQTGT 150
    SSLHVYDGKF LARVERVIVV SMNYRVGALG FLALPGNPEA PGNVGLFDQQ 200
    LALQWVQKNI AAFGGNPKSV TLFGESAGAA SVSLHLLSPE SHPLFTRAIL 250
    QSGSSNAPWA VTSRYEARNR TLTLAKFIGC SRENDTEIIK CLRNKDPQEI 300
    LRHEVFVVPY GTLLSVNFGP TVDGDFLTDM PDTLLQLGQF KKTQILVGVN 350
    KDEGTAFLVY GAPGFSKDNN SIITRKEFQE GLKIFFPGVS EFGKESILFH 400
    YMDWLDDQRA EKYREALDDV VGDYNIICPA LEFTKKFSDM GNNAFFYYFE 450
    HRSSKLPWPE WMGVMHGYEI EFVFGLPLER RVNYTKAEEI FSRSIMKRWA 500
    NFAKYGNPNG TQNNSTRWPV FKSNEQKYFT LNTESPKVNT KLRAQQCRFW 550
    TLFFPKVLEI TGNIDEVERE WKAGFHRWNN YMMDWKNQFN DYTSKKESCA 600
    GL 602
    Length:602
    Mass (Da):68,867
    Last modified:April 3, 2007 - v2
    Checksum:iCDFB404E64C46935
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC123600 mRNA. Translation: AAI23601.1.
    M62410 Genomic DNA. Translation: AAA51412.1.
    PIRiF39768.
    RefSeqiNP_001070374.1. NM_001076906.1.
    UniGeneiBt.42172.

    Genome annotation databases

    EnsembliENSBTAT00000014794; ENSBTAP00000014794; ENSBTAG00000011139.
    GeneIDi534616.
    KEGGibta:534616.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC123600 mRNA. Translation: AAI23601.1 .
    M62410 Genomic DNA. Translation: AAA51412.1 .
    PIRi F39768.
    RefSeqi NP_001070374.1. NM_001076906.1.
    UniGenei Bt.42172.

    3D structure databases

    ProteinModelPortali P32749.
    SMRi P32749. Positions 32-562, 564-593.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000014794.

    Protein family/group databases

    MEROPSi S09.980.

    Proteomic databases

    PRIDEi P32749.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000014794 ; ENSBTAP00000014794 ; ENSBTAG00000011139 .
    GeneIDi 534616.
    KEGGi bta:534616.

    Organism-specific databases

    CTDi 590.

    Phylogenomic databases

    eggNOGi COG2272.
    GeneTreei ENSGT00740000115241.
    HOGENOMi HOG000091866.
    HOVERGENi HBG008839.
    InParanoidi P32749.
    KOi K01050.
    OMAi WIPTPKP.
    OrthoDBi EOG789C9R.
    TreeFami TF315470.

    Enzyme and pathway databases

    Reactomei REACT_223503. Synthesis, secretion, and deacylation of Ghrelin.

    Miscellaneous databases

    NextBioi 20876472.

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR014788. AChE_tetra.
    IPR002018. CarbesteraseB.
    IPR019826. Carboxylesterase_B_AS.
    IPR019819. Carboxylesterase_B_CS.
    IPR000997. Cholinesterase.
    [Graphical view ]
    Pfami PF08674. AChE_tetra. 1 hit.
    PF00135. COesterase. 1 hit.
    [Graphical view ]
    PRINTSi PR00878. CHOLNESTRASE.
    ProDomi PD415333. AChE_tetra. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF53474. SSF53474. 1 hit.
    PROSITEi PS00122. CARBOXYLESTERASE_B_1. 1 hit.
    PS00941. CARBOXYLESTERASE_B_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Fetal cerebellum.
    2. "Use of the polymerase chain reaction for homology probing of butyrylcholinesterase from several vertebrates."
      Arpagaus M., Chatonnet A., Masson P., Newton M., Vaughan T.A., Bartels C.F., Nogueira C.P., la Du B.N., Lockridge O.
      J. Biol. Chem. 266:6966-6974(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 96-236.
      Tissue: Lung.

    Entry informationi

    Entry nameiCHLE_BOVIN
    AccessioniPrimary (citable) accession number: P32749
    Secondary accession number(s): Q08DR6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: April 3, 2007
    Last modified: October 1, 2014
    This is version 101 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3