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Protein

Cholinesterase

Gene

BCHE

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters (By similarity).By similarity

Catalytic activityi

An acylcholine + H2O = choline + a carboxylate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei226 – 2261Acyl-ester intermediatePROSITE-ProRule annotation
Active sitei353 – 3531Charge relay systemBy similarity
Active sitei466 – 4661Charge relay systemBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Enzyme and pathway databases

ReactomeiREACT_280970. Synthesis, secretion, and deacylation of Ghrelin.

Protein family/group databases

ESTHERibovin-BCHE. BCHE.
MEROPSiS09.980.

Names & Taxonomyi

Protein namesi
Recommended name:
Cholinesterase (EC:3.1.1.8)
Alternative name(s):
Acylcholine acylhydrolase
Butyrylcholine esterase
Choline esterase II
Pseudocholinesterase
Gene namesi
Name:BCHE
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 1

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence AnalysisAdd
BLAST
Chaini29 – 602574CholinesterasePRO_0000070283Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi85 – 851N-linked (GlcNAc...)Sequence Analysis
Glycosylationi134 – 1341N-linked (GlcNAc...)Sequence Analysis
Modified residuei226 – 2261PhosphoserineBy similarity
Glycosylationi269 – 2691N-linked (GlcNAc...)Sequence Analysis
Glycosylationi284 – 2841N-linked (GlcNAc...)Sequence Analysis
Glycosylationi369 – 3691N-linked (GlcNAc...)Sequence Analysis
Glycosylationi483 – 4831N-linked (GlcNAc...)Sequence Analysis
Glycosylationi509 – 5091N-linked (GlcNAc...)Sequence Analysis
Glycosylationi513 – 5131N-linked (GlcNAc...)Sequence Analysis
Glycosylationi514 – 5141N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiP32749.

Expressioni

Tissue specificityi

Present in most cells except erythrocytes.

Interactioni

Subunit structurei

Homotetramer; disulfide-linked. Dimer of dimers (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000014794.

Structurei

3D structure databases

ProteinModelPortaliP32749.
SMRiP32749. Positions 32-562, 564-593.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni144 – 1452Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2272.
GeneTreeiENSGT00760000118946.
HOGENOMiHOG000091866.
HOVERGENiHBG008839.
InParanoidiP32749.
KOiK01050.
OMAiAGFYRWN.
OrthoDBiEOG789C9R.
TreeFamiTF315470.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR014788. AChE_tetra.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
[Graphical view]
PfamiPF08674. AChE_tetra. 1 hit.
PF00135. COesterase. 1 hit.
[Graphical view]
PRINTSiPR00878. CHOLNESTRASE.
ProDomiPD415333. AChE_tetra. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32749-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSRSTVIYI RFVLWFLLLW VLFEKSHTEE DIIITTKNGK VRGMHLPVLG
60 70 80 90 100
GTVTAFLGIP YAQPPLGRLR FKKPQSLTKW PDIWNATKYA NSCYQNTDQS
110 120 130 140 150
FPGFLGSEMW NPNTDLSEDC LYLNVWIPTP KPKNATVMIW IYGGSFQTGT
160 170 180 190 200
SSLHVYDGKF LARVERVIVV SMNYRVGALG FLALPGNPEA PGNVGLFDQQ
210 220 230 240 250
LALQWVQKNI AAFGGNPKSV TLFGESAGAA SVSLHLLSPE SHPLFTRAIL
260 270 280 290 300
QSGSSNAPWA VTSRYEARNR TLTLAKFIGC SRENDTEIIK CLRNKDPQEI
310 320 330 340 350
LRHEVFVVPY GTLLSVNFGP TVDGDFLTDM PDTLLQLGQF KKTQILVGVN
360 370 380 390 400
KDEGTAFLVY GAPGFSKDNN SIITRKEFQE GLKIFFPGVS EFGKESILFH
410 420 430 440 450
YMDWLDDQRA EKYREALDDV VGDYNIICPA LEFTKKFSDM GNNAFFYYFE
460 470 480 490 500
HRSSKLPWPE WMGVMHGYEI EFVFGLPLER RVNYTKAEEI FSRSIMKRWA
510 520 530 540 550
NFAKYGNPNG TQNNSTRWPV FKSNEQKYFT LNTESPKVNT KLRAQQCRFW
560 570 580 590 600
TLFFPKVLEI TGNIDEVERE WKAGFHRWNN YMMDWKNQFN DYTSKKESCA

GL
Length:602
Mass (Da):68,867
Last modified:April 3, 2007 - v2
Checksum:iCDFB404E64C46935
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC123600 mRNA. Translation: AAI23601.1.
M62410 Genomic DNA. Translation: AAA51412.1.
PIRiF39768.
RefSeqiNP_001070374.1. NM_001076906.1.
UniGeneiBt.42172.

Genome annotation databases

EnsembliENSBTAT00000014794; ENSBTAP00000014794; ENSBTAG00000011139.
GeneIDi534616.
KEGGibta:534616.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC123600 mRNA. Translation: AAI23601.1.
M62410 Genomic DNA. Translation: AAA51412.1.
PIRiF39768.
RefSeqiNP_001070374.1. NM_001076906.1.
UniGeneiBt.42172.

3D structure databases

ProteinModelPortaliP32749.
SMRiP32749. Positions 32-562, 564-593.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000014794.

Protein family/group databases

ESTHERibovin-BCHE. BCHE.
MEROPSiS09.980.

Proteomic databases

PRIDEiP32749.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000014794; ENSBTAP00000014794; ENSBTAG00000011139.
GeneIDi534616.
KEGGibta:534616.

Organism-specific databases

CTDi590.

Phylogenomic databases

eggNOGiCOG2272.
GeneTreeiENSGT00760000118946.
HOGENOMiHOG000091866.
HOVERGENiHBG008839.
InParanoidiP32749.
KOiK01050.
OMAiAGFYRWN.
OrthoDBiEOG789C9R.
TreeFamiTF315470.

Enzyme and pathway databases

ReactomeiREACT_280970. Synthesis, secretion, and deacylation of Ghrelin.

Miscellaneous databases

NextBioi20876472.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR014788. AChE_tetra.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
[Graphical view]
PfamiPF08674. AChE_tetra. 1 hit.
PF00135. COesterase. 1 hit.
[Graphical view]
PRINTSiPR00878. CHOLNESTRASE.
ProDomiPD415333. AChE_tetra. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal cerebellum.
  2. "Use of the polymerase chain reaction for homology probing of butyrylcholinesterase from several vertebrates."
    Arpagaus M., Chatonnet A., Masson P., Newton M., Vaughan T.A., Bartels C.F., Nogueira C.P., la Du B.N., Lockridge O.
    J. Biol. Chem. 266:6966-6974(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 96-236.
    Tissue: Lung.

Entry informationi

Entry nameiCHLE_BOVIN
AccessioniPrimary (citable) accession number: P32749
Secondary accession number(s): Q08DR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: April 3, 2007
Last modified: July 22, 2015
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.