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P32749

- CHLE_BOVIN

UniProt

P32749 - CHLE_BOVIN

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Protein

Cholinesterase

Gene

BCHE

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters (By similarity).By similarity

Catalytic activityi

An acylcholine + H2O = choline + a carboxylate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei226 – 2261Acyl-ester intermediatePROSITE-ProRule annotation
Active sitei353 – 3531Charge relay systemBy similarity
Active sitei466 – 4661Charge relay systemBy similarity

GO - Molecular functioni

  1. acetylcholinesterase activity Source: UniProtKB
  2. cholinesterase activity Source: UniProtKB

GO - Biological processi

  1. choline metabolic process Source: RefGenome
  2. synaptic transmission, cholinergic Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Enzyme and pathway databases

ReactomeiREACT_223503. Synthesis, secretion, and deacylation of Ghrelin.

Protein family/group databases

MEROPSiS09.980.

Names & Taxonomyi

Protein namesi
Recommended name:
Cholinesterase (EC:3.1.1.8)
Alternative name(s):
Acylcholine acylhydrolase
Butyrylcholine esterase
Choline esterase II
Pseudocholinesterase
Gene namesi
Name:BCHE
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 1

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. blood microparticle Source: Ensembl
  2. endoplasmic reticulum lumen Source: RefGenome
  3. extracellular space Source: RefGenome
  4. nuclear envelope lumen Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence AnalysisAdd
BLAST
Chaini29 – 602574CholinesterasePRO_0000070283Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi85 – 851N-linked (GlcNAc...)Sequence Analysis
Glycosylationi134 – 1341N-linked (GlcNAc...)Sequence Analysis
Modified residuei226 – 2261PhosphoserineBy similarity
Glycosylationi269 – 2691N-linked (GlcNAc...)Sequence Analysis
Glycosylationi284 – 2841N-linked (GlcNAc...)Sequence Analysis
Glycosylationi369 – 3691N-linked (GlcNAc...)Sequence Analysis
Glycosylationi483 – 4831N-linked (GlcNAc...)Sequence Analysis
Glycosylationi509 – 5091N-linked (GlcNAc...)Sequence Analysis
Glycosylationi513 – 5131N-linked (GlcNAc...)Sequence Analysis
Glycosylationi514 – 5141N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiP32749.

Expressioni

Tissue specificityi

Present in most cells except erythrocytes.

Interactioni

Subunit structurei

Homotetramer; disulfide-linked. Dimer of dimers (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000014794.

Structurei

3D structure databases

ProteinModelPortaliP32749.
SMRiP32749. Positions 32-562, 564-593.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni144 – 1452Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2272.
GeneTreeiENSGT00760000118946.
HOGENOMiHOG000091866.
HOVERGENiHBG008839.
InParanoidiP32749.
KOiK01050.
OMAiWIPTPKP.
OrthoDBiEOG789C9R.
TreeFamiTF315470.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR014788. AChE_tetra.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
[Graphical view]
PfamiPF08674. AChE_tetra. 1 hit.
PF00135. COesterase. 1 hit.
[Graphical view]
PRINTSiPR00878. CHOLNESTRASE.
ProDomiPD415333. AChE_tetra. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32749-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQSRSTVIYI RFVLWFLLLW VLFEKSHTEE DIIITTKNGK VRGMHLPVLG
60 70 80 90 100
GTVTAFLGIP YAQPPLGRLR FKKPQSLTKW PDIWNATKYA NSCYQNTDQS
110 120 130 140 150
FPGFLGSEMW NPNTDLSEDC LYLNVWIPTP KPKNATVMIW IYGGSFQTGT
160 170 180 190 200
SSLHVYDGKF LARVERVIVV SMNYRVGALG FLALPGNPEA PGNVGLFDQQ
210 220 230 240 250
LALQWVQKNI AAFGGNPKSV TLFGESAGAA SVSLHLLSPE SHPLFTRAIL
260 270 280 290 300
QSGSSNAPWA VTSRYEARNR TLTLAKFIGC SRENDTEIIK CLRNKDPQEI
310 320 330 340 350
LRHEVFVVPY GTLLSVNFGP TVDGDFLTDM PDTLLQLGQF KKTQILVGVN
360 370 380 390 400
KDEGTAFLVY GAPGFSKDNN SIITRKEFQE GLKIFFPGVS EFGKESILFH
410 420 430 440 450
YMDWLDDQRA EKYREALDDV VGDYNIICPA LEFTKKFSDM GNNAFFYYFE
460 470 480 490 500
HRSSKLPWPE WMGVMHGYEI EFVFGLPLER RVNYTKAEEI FSRSIMKRWA
510 520 530 540 550
NFAKYGNPNG TQNNSTRWPV FKSNEQKYFT LNTESPKVNT KLRAQQCRFW
560 570 580 590 600
TLFFPKVLEI TGNIDEVERE WKAGFHRWNN YMMDWKNQFN DYTSKKESCA

GL
Length:602
Mass (Da):68,867
Last modified:April 3, 2007 - v2
Checksum:iCDFB404E64C46935
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC123600 mRNA. Translation: AAI23601.1.
M62410 Genomic DNA. Translation: AAA51412.1.
PIRiF39768.
RefSeqiNP_001070374.1. NM_001076906.1.
UniGeneiBt.42172.

Genome annotation databases

EnsembliENSBTAT00000014794; ENSBTAP00000014794; ENSBTAG00000011139.
GeneIDi534616.
KEGGibta:534616.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC123600 mRNA. Translation: AAI23601.1 .
M62410 Genomic DNA. Translation: AAA51412.1 .
PIRi F39768.
RefSeqi NP_001070374.1. NM_001076906.1.
UniGenei Bt.42172.

3D structure databases

ProteinModelPortali P32749.
SMRi P32749. Positions 32-562, 564-593.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000014794.

Protein family/group databases

MEROPSi S09.980.

Proteomic databases

PRIDEi P32749.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000014794 ; ENSBTAP00000014794 ; ENSBTAG00000011139 .
GeneIDi 534616.
KEGGi bta:534616.

Organism-specific databases

CTDi 590.

Phylogenomic databases

eggNOGi COG2272.
GeneTreei ENSGT00760000118946.
HOGENOMi HOG000091866.
HOVERGENi HBG008839.
InParanoidi P32749.
KOi K01050.
OMAi WIPTPKP.
OrthoDBi EOG789C9R.
TreeFami TF315470.

Enzyme and pathway databases

Reactomei REACT_223503. Synthesis, secretion, and deacylation of Ghrelin.

Miscellaneous databases

NextBioi 20876472.

Family and domain databases

Gene3Di 3.40.50.1820. 1 hit.
InterProi IPR029058. AB_hydrolase.
IPR014788. AChE_tetra.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
[Graphical view ]
Pfami PF08674. AChE_tetra. 1 hit.
PF00135. COesterase. 1 hit.
[Graphical view ]
PRINTSi PR00878. CHOLNESTRASE.
ProDomi PD415333. AChE_tetra. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF53474. SSF53474. 1 hit.
PROSITEi PS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal cerebellum.
  2. "Use of the polymerase chain reaction for homology probing of butyrylcholinesterase from several vertebrates."
    Arpagaus M., Chatonnet A., Masson P., Newton M., Vaughan T.A., Bartels C.F., Nogueira C.P., la Du B.N., Lockridge O.
    J. Biol. Chem. 266:6966-6974(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 96-236.
    Tissue: Lung.

Entry informationi

Entry nameiCHLE_BOVIN
AccessioniPrimary (citable) accession number: P32749
Secondary accession number(s): Q08DR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: April 3, 2007
Last modified: October 29, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3