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P32749 (CHLE_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cholinesterase

EC=3.1.1.8
Alternative name(s):
Acylcholine acylhydrolase
Butyrylcholine esterase
Choline esterase II
Pseudocholinesterase
Gene names
Name:BCHE
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length602 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters By similarity.

Catalytic activity

An acylcholine + H2O = choline + a carboxylate.

Subunit structure

Homotetramer; disulfide-linked. Dimer of dimers By similarity.

Subcellular location

Secreted By similarity.

Tissue specificity

Present in most cells except erythrocytes.

Sequence similarities

Belongs to the type-B carboxylesterase/lipase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Potential
Chain29 – 602574Cholinesterase
PRO_0000070283

Regions

Region144 – 1452Substrate binding By similarity

Sites

Active site2261Acyl-ester intermediate By similarity
Active site3531Charge relay system By similarity
Active site4661Charge relay system By similarity

Amino acid modifications

Modified residue2261Phosphoserine By similarity
Glycosylation851N-linked (GlcNAc...) Potential
Glycosylation1341N-linked (GlcNAc...) Potential
Glycosylation2691N-linked (GlcNAc...) Potential
Glycosylation2841N-linked (GlcNAc...) Potential
Glycosylation3691N-linked (GlcNAc...) Potential
Glycosylation4831N-linked (GlcNAc...) Potential
Glycosylation5091N-linked (GlcNAc...) Potential
Glycosylation5131N-linked (GlcNAc...) Potential
Glycosylation5141N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P32749 [UniParc].

Last modified April 3, 2007. Version 2.
Checksum: CDFB404E64C46935

FASTA60268,867
        10         20         30         40         50         60 
MQSRSTVIYI RFVLWFLLLW VLFEKSHTEE DIIITTKNGK VRGMHLPVLG GTVTAFLGIP 

        70         80         90        100        110        120 
YAQPPLGRLR FKKPQSLTKW PDIWNATKYA NSCYQNTDQS FPGFLGSEMW NPNTDLSEDC 

       130        140        150        160        170        180 
LYLNVWIPTP KPKNATVMIW IYGGSFQTGT SSLHVYDGKF LARVERVIVV SMNYRVGALG 

       190        200        210        220        230        240 
FLALPGNPEA PGNVGLFDQQ LALQWVQKNI AAFGGNPKSV TLFGESAGAA SVSLHLLSPE 

       250        260        270        280        290        300 
SHPLFTRAIL QSGSSNAPWA VTSRYEARNR TLTLAKFIGC SRENDTEIIK CLRNKDPQEI 

       310        320        330        340        350        360 
LRHEVFVVPY GTLLSVNFGP TVDGDFLTDM PDTLLQLGQF KKTQILVGVN KDEGTAFLVY 

       370        380        390        400        410        420 
GAPGFSKDNN SIITRKEFQE GLKIFFPGVS EFGKESILFH YMDWLDDQRA EKYREALDDV 

       430        440        450        460        470        480 
VGDYNIICPA LEFTKKFSDM GNNAFFYYFE HRSSKLPWPE WMGVMHGYEI EFVFGLPLER 

       490        500        510        520        530        540 
RVNYTKAEEI FSRSIMKRWA NFAKYGNPNG TQNNSTRWPV FKSNEQKYFT LNTESPKVNT 

       550        560        570        580        590        600 
KLRAQQCRFW TLFFPKVLEI TGNIDEVERE WKAGFHRWNN YMMDWKNQFN DYTSKKESCA 


GL 

« Hide

References

« Hide 'large scale' references
[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Fetal cerebellum.
[2]"Use of the polymerase chain reaction for homology probing of butyrylcholinesterase from several vertebrates."
Arpagaus M., Chatonnet A., Masson P., Newton M., Vaughan T.A., Bartels C.F., Nogueira C.P., la Du B.N., Lockridge O.
J. Biol. Chem. 266:6966-6974(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 96-236.
Tissue: Lung.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC123600 mRNA. Translation: AAI23601.1.
M62410 Genomic DNA. Translation: AAA51412.1.
PIRF39768.
RefSeqNP_001070374.1. NM_001076906.1.
UniGeneBt.42172.

3D structure databases

ProteinModelPortalP32749.
SMRP32749. Positions 32-562, 564-593.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000014794.

Protein family/group databases

MEROPSS09.980.

Proteomic databases

PRIDEP32749.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000014794; ENSBTAP00000014794; ENSBTAG00000011139.
GeneID534616.
KEGGbta:534616.

Organism-specific databases

CTD590.

Phylogenomic databases

eggNOGCOG2272.
GeneTreeENSGT00740000115241.
HOGENOMHOG000091866.
HOVERGENHBG008839.
InParanoidP32749.
KOK01050.
OMAWIPTPKP.
OrthoDBEOG789C9R.
TreeFamTF315470.

Family and domain databases

Gene3D3.40.50.1820. 1 hit.
InterProIPR029058. AB_hydrolase.
IPR014788. AChE_tetra.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
[Graphical view]
PfamPF08674. AChE_tetra. 1 hit.
PF00135. COesterase. 1 hit.
[Graphical view]
PRINTSPR00878. CHOLNESTRASE.
ProDomPD415333. AChE_tetra. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF53474. SSF53474. 1 hit.
PROSITEPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20876472.

Entry information

Entry nameCHLE_BOVIN
AccessionPrimary (citable) accession number: P32749
Secondary accession number(s): Q08DR6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: April 3, 2007
Last modified: June 11, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families