P32722 (PORD_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Porin D
EC=3.4.21.-

Alternative name(s):
Imipenem/basic amino acid-specific outer membrane pore
Outer membrane protein D2

Gene names

Name:	oprD
Ordered Locus Names:	PA0958

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Porin with a specificity for basic amino acids. Also possesses serine protease activity. Ref.5 Ref.6
Subcellular location	Cell outer membrane; Multi-pass membrane protein Ref.5.
Sequence similarities	Belongs to the outer membrane porin (Opr) (TC 1.B.25) family. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell membrane Cell outer membrane Membrane
Domain	Signal Transmembrane Transmembrane beta strand
Molecular function	Hydrolase Porin Protease Serine protease
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	basic amino acid transport Inferred from direct assay Ref.5. Source: UniProtKB proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to cell outer membrane Inferred from direct assay Ref.4. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW pore complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	porin activity Inferred from direct assay Ref.5. Source: UniProtKB serine-type peptidase activity Inferred from direct assay Ref.6. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 23

23

Ref.2 Ref.4

Chain

24 – 443

420

Porin D

PRO_0000027336

Sites

Active site

179

1

Ref.7

Active site

231

1

Ref.7

Active site

319

1

Ref.7

Experimental info

Mutagenesis

179

1

H → Q: Loss of protease activity. No effect on porin activity. Ref.7

Mutagenesis

231

1

D → N: Loss of protease activity. No effect on porin activity. Ref.7

Mutagenesis

319

1

S → A: Loss of protease activity. No effect on porin activity. Ref.7

Mutagenesis

390

1

H → Q: No effect on protease activity. Ref.7

Sequence conflict

44

1

L → Y AA sequence Ref.2

Secondary structure

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443

Helix Strand Turn

Details...

Helix

30 – 32

3

Turn

36 – 39

4

Beta strand

41 – 54

14

Beta strand

62 – 74

13

Beta strand

79 – 98

20

Beta strand

119 – 131

13

Beta strand

134 – 141

8

Beta strand

153 – 156

4

Beta strand

160 – 167

8

Beta strand

173 – 182

10

Beta strand

194 – 197

4

Turn

198 – 201

4

Beta strand

202 – 217

16

Beta strand

220 – 229

10

Turn

230 – 232

3

Beta strand

233 – 247

15

Beta strand

250 – 263

14

Beta strand

273 – 285

13

Beta strand

288 – 301

14

Beta strand

306 – 308

3

Beta strand

337 – 346

10

Turn

348 – 351

4

Beta strand

355 – 367

13

Helix

377 – 381

5

Beta strand

386 – 400

15

Turn

405 – 408

4

Beta strand

410 – 420

11

Helix

422 – 424

3

Beta strand

429 – 439

11

Sequences

	Sequence		Length	Mass (Da)
	P32722 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: E083FFE074DCFB64	FASTA	443	48,360
	10 20 30 40 50 60 MKVMKWSAIA LAVSAGSTQF AVADAFVSDQ AEAKGFIEDS SLDLLLRNYY FNRDGKSGSG 70 80 90 100 110 120 DRVDWTQGFL TTYESGFTQG TVGFGVDAFG YLGLKLDGTS DKTGTGNLPV MNDGKPRDDY 130 140 150 160 170 180 SRAGGAVKVR ISKTMLKWGE MQPTAPVFAA GGSRLFPQTA TGFQLQSSEF EGLDLEAGHF 190 200 210 220 230 240 TEGKEPTTVK SRGELYATYA GETAKSADFI GGRYAITDNL SASLYGAELE DIYRQYYLNS 250 260 270 280 290 300 NYTIPLASDQ SLGFDFNIYR TNDEGKAKAG DISNTTWSLA AAYTLDAHTF TLAYQKVHGD 310 320 330 340 350 360 QPFDYIGFGR NGSGAGGDSI FLANSVQYSD FNGPGEKSWQ ARYDLNLASY GVPGLTFMVR 370 380 390 400 410 420 YINGKDIDGT KMSDNNVGYK NYGYGEDGKH HETNLEAKYV VQSGPAKDLS FRIRQAWHRA 430 440 NADQGEGDQN EFRLIVDYPL SIL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the protein D2 gene of Pseudomonas aeruginosa." Yoneyama H., Yoshihara E., Nakae T. Antimicrob. Agents Chemother. 36:1791-1793(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of two gene regions involved in the expression of the imipenem-specific, outer membrane porin protein OprD of Pseudomonas aeruginosa." Huang H., Siehnel R.J., Bellido F., Rawling E., Hancock R.E.W. FEMS Microbiol. Lett. 76:267-274(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 24-40. Strain: ATCC 15692 / PAO1 / H103.
[3]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[4]	"Two-dimensional polyacrylamide gel electrophoresis isolation and microsequencing of Pseudomonas aeruginosa proteins." Michea-Hamzehpour M., Sanchez J.-C., Epp S.F., Paquet N., Hughes G.J., Hochstrasser D.F., Pechere J.-C. Enzyme Protein 47:1-8(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 24-48.
[5]	"Protein D2 channel of the Pseudomonas aeruginosa outer membrane has a binding site for basic amino acids and peptides." Trias J., Nikaido H. J. Biol. Chem. 265:15680-15684(1990) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"Protein D2 porin of the Pseudomonas aeruginosa outer membrane bears the protease activity." Yoshihara E., Gotoh N., Nishino T., Nakae T. FEBS Lett. 394:179-182(1996) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A SERINE PROTEASE.
[7]	"Identification of the catalytic triad of the protein D2 protease in Pseudomonas aeruginosa." Yoshihara E., Yoneyama H., Ono T., Nakae T. Biochem. Biophys. Res. Commun. 247:142-145(1998) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF HIS-179; ASP-231; SER-319 AND HIS-390, ACTIVE SITES.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X63152 Genomic DNA. Translation: CAA44855.1.
Z14065 Genomic DNA. Translation: CAA78448.1.
AE004091 Genomic DNA. Translation: AAG04347.1.

PIR

S23771.

RefSeq

NP_249649.1. NC_002516.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2ODJ	X-ray	2.90	A/B	26-443	[»]
3SY7	X-ray	2.15	A	24-443	[»]
4FOZ	X-ray	2.40	A	24-443	[»]

ProteinModelPortal

P32722.

SMR

P32722. Positions 24-443.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-29627N.

STRING

208964.PA0958.

Protein family/group databases

TCDB

1.B.25.1.1. outer membrane porin (Opr) family.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

881970.

KEGG

pae:PA0958.

PATRIC

19836216. VBIPseAer58763_0996.

Organism-specific databases

PseudoCAP

PA0958.

Phylogenomic databases

eggNOG

NOG14525.

HOGENOM

HOG000220145.

OMA

HTFTLAY.

ProtClustDB

CLSK866247.

Family and domain databases

Gene3D

2.40.160.10. 1 hit.

InterPro

IPR005318. OM_porin_bac.
IPR023614. Porin_dom.
[Graphical view]

Pfam

PF03573. OprD. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P32722.

Entry information

Entry name

PORD_PSEAE

Accession

Primary (citable) accession number: P32722

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program