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P32722

- PORD_PSEAE

UniProt

P32722 - PORD_PSEAE

Protein

Porin D

Gene

oprD

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Porin with a specificity for basic amino acids. Also possesses serine protease activity.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei179 – 17911 Publication
    Active sitei231 – 23111 Publication
    Active sitei319 – 31911 Publication

    GO - Molecular functioni

    1. porin activity Source: UniProtKB
    2. serine-type peptidase activity Source: UniProtKB

    GO - Biological processi

    1. basic amino acid transport Source: UniProtKB
    2. transmembrane transport Source: GOC

    Keywords - Molecular functioni

    Hydrolase, Porin, Protease, Serine protease

    Keywords - Biological processi

    Ion transport, Transport

    Protein family/group databases

    TCDBi1.B.25.1.1. the outer membrane porin (opr) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Porin D (EC:3.4.21.-)
    Alternative name(s):
    Imipenem/basic amino acid-specific outer membrane pore
    Outer membrane protein D2
    Gene namesi
    Name:oprD
    Ordered Locus Names:PA0958
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002438: Chromosome

    Organism-specific databases

    PseudoCAPiPA0958.

    Subcellular locationi

    Cell outer membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of cell outer membrane Source: UniProtKB
    2. outer membrane Source: PseudoCAP
    3. pore complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell outer membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi179 – 1791H → Q: Loss of protease activity. No effect on porin activity. 1 Publication
    Mutagenesisi231 – 2311D → N: Loss of protease activity. No effect on porin activity. 1 Publication
    Mutagenesisi319 – 3191S → A: Loss of protease activity. No effect on porin activity. 1 Publication
    Mutagenesisi390 – 3901H → Q: No effect on protease activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 23232 PublicationsAdd
    BLAST
    Chaini24 – 443420Porin DPRO_0000027336Add
    BLAST

    Proteomic databases

    PRIDEiP32722.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-29627N.
    STRINGi208964.PA0958.

    Structurei

    Secondary structure

    1
    443
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi30 – 323
    Turni36 – 394
    Beta strandi41 – 5414
    Beta strandi62 – 7413
    Beta strandi79 – 9820
    Beta strandi119 – 13113
    Beta strandi134 – 1418
    Beta strandi153 – 1564
    Beta strandi160 – 1678
    Beta strandi173 – 18210
    Beta strandi194 – 1974
    Turni198 – 2014
    Beta strandi202 – 21716
    Beta strandi220 – 22910
    Turni230 – 2323
    Beta strandi233 – 24715
    Beta strandi250 – 26314
    Beta strandi273 – 28513
    Beta strandi288 – 30114
    Beta strandi306 – 3083
    Beta strandi337 – 34610
    Turni348 – 3514
    Beta strandi355 – 36713
    Helixi377 – 3815
    Beta strandi386 – 40015
    Turni405 – 4084
    Beta strandi410 – 42011
    Helixi422 – 4243
    Beta strandi429 – 43911

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2ODJX-ray2.90A/B26-443[»]
    3SY7X-ray2.15A24-443[»]
    4FOZX-ray2.40A24-443[»]
    ProteinModelPortaliP32722.
    SMRiP32722. Positions 24-443.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP32722.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane beta strand

    Phylogenomic databases

    eggNOGiNOG14525.
    HOGENOMiHOG000220145.
    KOiK18093.
    OMAiHTFTLAY.
    OrthoDBiEOG63Z742.
    PhylomeDBiP32722.

    Family and domain databases

    Gene3Di2.40.160.10. 1 hit.
    InterProiIPR005318. OM_porin_bac.
    IPR023614. Porin_dom.
    [Graphical view]
    PfamiPF03573. OprD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P32722-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKVMKWSAIA LAVSAGSTQF AVADAFVSDQ AEAKGFIEDS SLDLLLRNYY    50
    FNRDGKSGSG DRVDWTQGFL TTYESGFTQG TVGFGVDAFG YLGLKLDGTS 100
    DKTGTGNLPV MNDGKPRDDY SRAGGAVKVR ISKTMLKWGE MQPTAPVFAA 150
    GGSRLFPQTA TGFQLQSSEF EGLDLEAGHF TEGKEPTTVK SRGELYATYA 200
    GETAKSADFI GGRYAITDNL SASLYGAELE DIYRQYYLNS NYTIPLASDQ 250
    SLGFDFNIYR TNDEGKAKAG DISNTTWSLA AAYTLDAHTF TLAYQKVHGD 300
    QPFDYIGFGR NGSGAGGDSI FLANSVQYSD FNGPGEKSWQ ARYDLNLASY 350
    GVPGLTFMVR YINGKDIDGT KMSDNNVGYK NYGYGEDGKH HETNLEAKYV 400
    VQSGPAKDLS FRIRQAWHRA NADQGEGDQN EFRLIVDYPL SIL 443
    Length:443
    Mass (Da):48,360
    Last modified:October 1, 1993 - v1
    Checksum:iE083FFE074DCFB64
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti44 – 441L → Y AA sequence (PubMed:1427017)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X63152 Genomic DNA. Translation: CAA44855.1.
    Z14065 Genomic DNA. Translation: CAA78448.1.
    AE004091 Genomic DNA. Translation: AAG04347.1.
    PIRiS23771.
    RefSeqiNP_249649.1. NC_002516.2.

    Genome annotation databases

    EnsemblBacteriaiAAG04347; AAG04347; PA0958.
    GeneIDi881970.
    KEGGipae:PA0958.
    PATRICi19836216. VBIPseAer58763_0996.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X63152 Genomic DNA. Translation: CAA44855.1 .
    Z14065 Genomic DNA. Translation: CAA78448.1 .
    AE004091 Genomic DNA. Translation: AAG04347.1 .
    PIRi S23771.
    RefSeqi NP_249649.1. NC_002516.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2ODJ X-ray 2.90 A/B 26-443 [» ]
    3SY7 X-ray 2.15 A 24-443 [» ]
    4FOZ X-ray 2.40 A 24-443 [» ]
    ProteinModelPortali P32722.
    SMRi P32722. Positions 24-443.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-29627N.
    STRINGi 208964.PA0958.

    Protein family/group databases

    TCDBi 1.B.25.1.1. the outer membrane porin (opr) family.

    Proteomic databases

    PRIDEi P32722.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG04347 ; AAG04347 ; PA0958 .
    GeneIDi 881970.
    KEGGi pae:PA0958.
    PATRICi 19836216. VBIPseAer58763_0996.

    Organism-specific databases

    PseudoCAPi PA0958.

    Phylogenomic databases

    eggNOGi NOG14525.
    HOGENOMi HOG000220145.
    KOi K18093.
    OMAi HTFTLAY.
    OrthoDBi EOG63Z742.
    PhylomeDBi P32722.

    Miscellaneous databases

    EvolutionaryTracei P32722.

    Family and domain databases

    Gene3Di 2.40.160.10. 1 hit.
    InterProi IPR005318. OM_porin_bac.
    IPR023614. Porin_dom.
    [Graphical view ]
    Pfami PF03573. OprD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the protein D2 gene of Pseudomonas aeruginosa."
      Yoneyama H., Yoshihara E., Nakae T.
      Antimicrob. Agents Chemother. 36:1791-1793(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Analysis of two gene regions involved in the expression of the imipenem-specific, outer membrane porin protein OprD of Pseudomonas aeruginosa."
      Huang H., Siehnel R.J., Bellido F., Rawling E., Hancock R.E.W.
      FEMS Microbiol. Lett. 76:267-274(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 24-40.
      Strain: ATCC 15692 / PAO1 / H103.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
    4. "Two-dimensional polyacrylamide gel electrophoresis isolation and microsequencing of Pseudomonas aeruginosa proteins."
      Michea-Hamzehpour M., Sanchez J.-C., Epp S.F., Paquet N., Hughes G.J., Hochstrasser D.F., Pechere J.-C.
      Enzyme Protein 47:1-8(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 24-48.
    5. "Protein D2 channel of the Pseudomonas aeruginosa outer membrane has a binding site for basic amino acids and peptides."
      Trias J., Nikaido H.
      J. Biol. Chem. 265:15680-15684(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    6. "Protein D2 porin of the Pseudomonas aeruginosa outer membrane bears the protease activity."
      Yoshihara E., Gotoh N., Nishino T., Nakae T.
      FEBS Lett. 394:179-182(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A SERINE PROTEASE.
    7. "Identification of the catalytic triad of the protein D2 protease in Pseudomonas aeruginosa."
      Yoshihara E., Yoneyama H., Ono T., Nakae T.
      Biochem. Biophys. Res. Commun. 247:142-145(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF HIS-179; ASP-231; SER-319 AND HIS-390, ACTIVE SITES.

    Entry informationi

    Entry nameiPORD_PSEAE
    AccessioniPrimary (citable) accession number: P32722
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3