Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Porin D

Gene

oprD

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Porin with a specificity for basic amino acids. Also possesses serine protease activity.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1791 Publication1
Active sitei2311 Publication1
Active sitei3191 Publication1

GO - Molecular functioni

  • porin activity Source: UniProtKB
  • serine-type peptidase activity Source: UniProtKB

GO - Biological processi

  • basic amino acid transport Source: UniProtKB
  • ion transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Porin, Protease, Serine protease

Keywords - Biological processi

Ion transport, Transport

Protein family/group databases

TCDBi1.B.25.1.1. the outer membrane porin (opr) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Porin D (EC:3.4.21.-)
Alternative name(s):
Imipenem/basic amino acid-specific outer membrane pore
Outer membrane protein D2
Gene namesi
Name:oprD
Ordered Locus Names:PA0958
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA0958.

Subcellular locationi

  • Cell outer membrane 1 Publication; Multi-pass membrane protein 1 Publication

GO - Cellular componenti

  • integral component of cell outer membrane Source: UniProtKB
  • outer membrane Source: PseudoCAP
  • pore complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi179H → Q: Loss of protease activity. No effect on porin activity. 1 Publication1
Mutagenesisi231D → N: Loss of protease activity. No effect on porin activity. 1 Publication1
Mutagenesisi319S → A: Loss of protease activity. No effect on porin activity. 1 Publication1
Mutagenesisi390H → Q: No effect on protease activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 232 PublicationsAdd BLAST23
ChainiPRO_000002733624 – 443Porin DAdd BLAST420

Proteomic databases

PaxDbiP32722.
PRIDEiP32722.

Interactioni

Protein-protein interaction databases

DIPiDIP-29627N.
STRINGi208964.PA0958.

Structurei

Secondary structure

1443
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi30 – 32Combined sources3
Turni36 – 39Combined sources4
Beta strandi41 – 54Combined sources14
Beta strandi62 – 74Combined sources13
Beta strandi79 – 98Combined sources20
Beta strandi119 – 131Combined sources13
Beta strandi134 – 141Combined sources8
Beta strandi153 – 156Combined sources4
Beta strandi160 – 167Combined sources8
Beta strandi173 – 182Combined sources10
Beta strandi194 – 197Combined sources4
Turni198 – 201Combined sources4
Beta strandi202 – 217Combined sources16
Beta strandi220 – 229Combined sources10
Turni230 – 232Combined sources3
Beta strandi233 – 247Combined sources15
Beta strandi250 – 263Combined sources14
Beta strandi273 – 285Combined sources13
Beta strandi288 – 301Combined sources14
Beta strandi306 – 308Combined sources3
Beta strandi337 – 346Combined sources10
Turni348 – 351Combined sources4
Beta strandi355 – 367Combined sources13
Helixi377 – 381Combined sources5
Beta strandi386 – 400Combined sources15
Turni405 – 408Combined sources4
Beta strandi410 – 420Combined sources11
Helixi422 – 424Combined sources3
Beta strandi429 – 439Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ODJX-ray2.90A/B26-443[»]
3SY7X-ray2.15A24-443[»]
4FOZX-ray2.40A24-443[»]
ProteinModelPortaliP32722.
SMRiP32722.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32722.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105J5J. Bacteria.
ENOG410XSR4. LUCA.
HOGENOMiHOG000220145.
InParanoidiP32722.
KOiK18093.
OMAiHTFTLAY.
PhylomeDBiP32722.

Family and domain databases

Gene3Di2.40.160.10. 1 hit.
InterProiIPR005318. OM_porin_bac.
IPR023614. Porin_dom.
[Graphical view]
PfamiPF03573. OprD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32722-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVMKWSAIA LAVSAGSTQF AVADAFVSDQ AEAKGFIEDS SLDLLLRNYY
60 70 80 90 100
FNRDGKSGSG DRVDWTQGFL TTYESGFTQG TVGFGVDAFG YLGLKLDGTS
110 120 130 140 150
DKTGTGNLPV MNDGKPRDDY SRAGGAVKVR ISKTMLKWGE MQPTAPVFAA
160 170 180 190 200
GGSRLFPQTA TGFQLQSSEF EGLDLEAGHF TEGKEPTTVK SRGELYATYA
210 220 230 240 250
GETAKSADFI GGRYAITDNL SASLYGAELE DIYRQYYLNS NYTIPLASDQ
260 270 280 290 300
SLGFDFNIYR TNDEGKAKAG DISNTTWSLA AAYTLDAHTF TLAYQKVHGD
310 320 330 340 350
QPFDYIGFGR NGSGAGGDSI FLANSVQYSD FNGPGEKSWQ ARYDLNLASY
360 370 380 390 400
GVPGLTFMVR YINGKDIDGT KMSDNNVGYK NYGYGEDGKH HETNLEAKYV
410 420 430 440
VQSGPAKDLS FRIRQAWHRA NADQGEGDQN EFRLIVDYPL SIL
Length:443
Mass (Da):48,360
Last modified:October 1, 1993 - v1
Checksum:iE083FFE074DCFB64
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti44L → Y AA sequence (PubMed:1427017).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63152 Genomic DNA. Translation: CAA44855.1.
Z14065 Genomic DNA. Translation: CAA78448.1.
AE004091 Genomic DNA. Translation: AAG04347.1.
PIRiS23771.
RefSeqiNP_249649.1. NC_002516.2.
WP_003112576.1. NZ_ASJY01000132.1.

Genome annotation databases

EnsemblBacteriaiAAG04347; AAG04347; PA0958.
GeneIDi881970.
KEGGipae:PA0958.
PATRICi19836216. VBIPseAer58763_0996.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63152 Genomic DNA. Translation: CAA44855.1.
Z14065 Genomic DNA. Translation: CAA78448.1.
AE004091 Genomic DNA. Translation: AAG04347.1.
PIRiS23771.
RefSeqiNP_249649.1. NC_002516.2.
WP_003112576.1. NZ_ASJY01000132.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ODJX-ray2.90A/B26-443[»]
3SY7X-ray2.15A24-443[»]
4FOZX-ray2.40A24-443[»]
ProteinModelPortaliP32722.
SMRiP32722.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29627N.
STRINGi208964.PA0958.

Protein family/group databases

TCDBi1.B.25.1.1. the outer membrane porin (opr) family.

Proteomic databases

PaxDbiP32722.
PRIDEiP32722.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG04347; AAG04347; PA0958.
GeneIDi881970.
KEGGipae:PA0958.
PATRICi19836216. VBIPseAer58763_0996.

Organism-specific databases

PseudoCAPiPA0958.

Phylogenomic databases

eggNOGiENOG4105J5J. Bacteria.
ENOG410XSR4. LUCA.
HOGENOMiHOG000220145.
InParanoidiP32722.
KOiK18093.
OMAiHTFTLAY.
PhylomeDBiP32722.

Miscellaneous databases

EvolutionaryTraceiP32722.

Family and domain databases

Gene3Di2.40.160.10. 1 hit.
InterProiIPR005318. OM_porin_bac.
IPR023614. Porin_dom.
[Graphical view]
PfamiPF03573. OprD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPORD_PSEAE
AccessioniPrimary (citable) accession number: P32722
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 2, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.