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P32722

- PORD_PSEAE

UniProt

P32722 - PORD_PSEAE

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Protein

Porin D

Gene

oprD

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Porin with a specificity for basic amino acids. Also possesses serine protease activity.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei179 – 17911 Publication
Active sitei231 – 23111 Publication
Active sitei319 – 31911 Publication

GO - Molecular functioni

  1. porin activity Source: UniProtKB
  2. serine-type peptidase activity Source: UniProtKB

GO - Biological processi

  1. basic amino acid transport Source: UniProtKB
  2. transmembrane transport Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Porin, Protease, Serine protease

Keywords - Biological processi

Ion transport, Transport

Protein family/group databases

TCDBi1.B.25.1.1. the outer membrane porin (opr) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Porin D (EC:3.4.21.-)
Alternative name(s):
Imipenem/basic amino acid-specific outer membrane pore
Outer membrane protein D2
Gene namesi
Name:oprD
Ordered Locus Names:PA0958
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438: Chromosome

Organism-specific databases

PseudoCAPiPA0958.

Subcellular locationi

Cell outer membrane 1 Publication; Multi-pass membrane protein 1 Publication

GO - Cellular componenti

  1. integral component of cell outer membrane Source: UniProtKB
  2. outer membrane Source: PseudoCAP
  3. pore complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi179 – 1791H → Q: Loss of protease activity. No effect on porin activity. 1 Publication
Mutagenesisi231 – 2311D → N: Loss of protease activity. No effect on porin activity. 1 Publication
Mutagenesisi319 – 3191S → A: Loss of protease activity. No effect on porin activity. 1 Publication
Mutagenesisi390 – 3901H → Q: No effect on protease activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23232 PublicationsAdd
BLAST
Chaini24 – 443420Porin DPRO_0000027336Add
BLAST

Proteomic databases

PRIDEiP32722.

Interactioni

Protein-protein interaction databases

DIPiDIP-29627N.
STRINGi208964.PA0958.

Structurei

Secondary structure

1
443
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi30 – 323
Turni36 – 394
Beta strandi41 – 5414
Beta strandi62 – 7413
Beta strandi79 – 9820
Beta strandi119 – 13113
Beta strandi134 – 1418
Beta strandi153 – 1564
Beta strandi160 – 1678
Beta strandi173 – 18210
Beta strandi194 – 1974
Turni198 – 2014
Beta strandi202 – 21716
Beta strandi220 – 22910
Turni230 – 2323
Beta strandi233 – 24715
Beta strandi250 – 26314
Beta strandi273 – 28513
Beta strandi288 – 30114
Beta strandi306 – 3083
Beta strandi337 – 34610
Turni348 – 3514
Beta strandi355 – 36713
Helixi377 – 3815
Beta strandi386 – 40015
Turni405 – 4084
Beta strandi410 – 42011
Helixi422 – 4243
Beta strandi429 – 43911

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ODJX-ray2.90A/B26-443[»]
3SY7X-ray2.15A24-443[»]
4FOZX-ray2.40A24-443[»]
ProteinModelPortaliP32722.
SMRiP32722. Positions 24-443.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32722.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiNOG14525.
HOGENOMiHOG000220145.
InParanoidiP32722.
KOiK18093.
OMAiHTFTLAY.
OrthoDBiEOG63Z742.
PhylomeDBiP32722.

Family and domain databases

Gene3Di2.40.160.10. 1 hit.
InterProiIPR005318. OM_porin_bac.
IPR023614. Porin_dom.
[Graphical view]
PfamiPF03573. OprD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32722-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVMKWSAIA LAVSAGSTQF AVADAFVSDQ AEAKGFIEDS SLDLLLRNYY
60 70 80 90 100
FNRDGKSGSG DRVDWTQGFL TTYESGFTQG TVGFGVDAFG YLGLKLDGTS
110 120 130 140 150
DKTGTGNLPV MNDGKPRDDY SRAGGAVKVR ISKTMLKWGE MQPTAPVFAA
160 170 180 190 200
GGSRLFPQTA TGFQLQSSEF EGLDLEAGHF TEGKEPTTVK SRGELYATYA
210 220 230 240 250
GETAKSADFI GGRYAITDNL SASLYGAELE DIYRQYYLNS NYTIPLASDQ
260 270 280 290 300
SLGFDFNIYR TNDEGKAKAG DISNTTWSLA AAYTLDAHTF TLAYQKVHGD
310 320 330 340 350
QPFDYIGFGR NGSGAGGDSI FLANSVQYSD FNGPGEKSWQ ARYDLNLASY
360 370 380 390 400
GVPGLTFMVR YINGKDIDGT KMSDNNVGYK NYGYGEDGKH HETNLEAKYV
410 420 430 440
VQSGPAKDLS FRIRQAWHRA NADQGEGDQN EFRLIVDYPL SIL
Length:443
Mass (Da):48,360
Last modified:October 1, 1993 - v1
Checksum:iE083FFE074DCFB64
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti44 – 441L → Y AA sequence (PubMed:1427017)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X63152 Genomic DNA. Translation: CAA44855.1.
Z14065 Genomic DNA. Translation: CAA78448.1.
AE004091 Genomic DNA. Translation: AAG04347.1.
PIRiS23771.
RefSeqiNP_249649.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG04347; AAG04347; PA0958.
GeneIDi881970.
KEGGipae:PA0958.
PATRICi19836216. VBIPseAer58763_0996.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X63152 Genomic DNA. Translation: CAA44855.1 .
Z14065 Genomic DNA. Translation: CAA78448.1 .
AE004091 Genomic DNA. Translation: AAG04347.1 .
PIRi S23771.
RefSeqi NP_249649.1. NC_002516.2.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2ODJ X-ray 2.90 A/B 26-443 [» ]
3SY7 X-ray 2.15 A 24-443 [» ]
4FOZ X-ray 2.40 A 24-443 [» ]
ProteinModelPortali P32722.
SMRi P32722. Positions 24-443.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-29627N.
STRINGi 208964.PA0958.

Protein family/group databases

TCDBi 1.B.25.1.1. the outer membrane porin (opr) family.

Proteomic databases

PRIDEi P32722.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG04347 ; AAG04347 ; PA0958 .
GeneIDi 881970.
KEGGi pae:PA0958.
PATRICi 19836216. VBIPseAer58763_0996.

Organism-specific databases

PseudoCAPi PA0958.

Phylogenomic databases

eggNOGi NOG14525.
HOGENOMi HOG000220145.
InParanoidi P32722.
KOi K18093.
OMAi HTFTLAY.
OrthoDBi EOG63Z742.
PhylomeDBi P32722.

Miscellaneous databases

EvolutionaryTracei P32722.

Family and domain databases

Gene3Di 2.40.160.10. 1 hit.
InterProi IPR005318. OM_porin_bac.
IPR023614. Porin_dom.
[Graphical view ]
Pfami PF03573. OprD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the protein D2 gene of Pseudomonas aeruginosa."
    Yoneyama H., Yoshihara E., Nakae T.
    Antimicrob. Agents Chemother. 36:1791-1793(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Analysis of two gene regions involved in the expression of the imipenem-specific, outer membrane porin protein OprD of Pseudomonas aeruginosa."
    Huang H., Siehnel R.J., Bellido F., Rawling E., Hancock R.E.W.
    FEMS Microbiol. Lett. 76:267-274(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 24-40.
    Strain: ATCC 15692 / PAO1 / H103.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  4. "Two-dimensional polyacrylamide gel electrophoresis isolation and microsequencing of Pseudomonas aeruginosa proteins."
    Michea-Hamzehpour M., Sanchez J.-C., Epp S.F., Paquet N., Hughes G.J., Hochstrasser D.F., Pechere J.-C.
    Enzyme Protein 47:1-8(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-48.
  5. "Protein D2 channel of the Pseudomonas aeruginosa outer membrane has a binding site for basic amino acids and peptides."
    Trias J., Nikaido H.
    J. Biol. Chem. 265:15680-15684(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. "Protein D2 porin of the Pseudomonas aeruginosa outer membrane bears the protease activity."
    Yoshihara E., Gotoh N., Nishino T., Nakae T.
    FEBS Lett. 394:179-182(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A SERINE PROTEASE.
  7. "Identification of the catalytic triad of the protein D2 protease in Pseudomonas aeruginosa."
    Yoshihara E., Yoneyama H., Ono T., Nakae T.
    Biochem. Biophys. Res. Commun. 247:142-145(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF HIS-179; ASP-231; SER-319 AND HIS-390, ACTIVE SITES.

Entry informationi

Entry nameiPORD_PSEAE
AccessioniPrimary (citable) accession number: P32722
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: October 29, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3